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The map label for this gene is gshB [H]

Identifier: 78778566

GI number: 78778566

Start: 172797

End: 173720

Strand: Reverse

Name: gshB [H]

Synonym: PMT9312_0180

Alternate gene names: 78778566

Gene position: 173720-172797 (Counterclockwise)

Preceding gene: 78778567

Following gene: 78778564

Centisome position: 10.16

GC content: 32.36

Gene sequence:

>924_bases
ATGAAATTTCTATTCGTAATAGATCCAATTAAAAATATAAATCCCTTAAAAGATTCAACTGCTGCTTTAATGCAAGCATC
ATCAAAAAAAAATATTGAAATCTGGAGTTGTACTCCTCAAGACCTTGAAGCTAGAGGTGATGAAGTATGGGCATCTTCCG
TAAAAGTTGAAGTAAATCCGTGGATTTCTTTCAAAGAGAATGATTGCATACCTCTCGCCGAATTTAATTGCATTTGGATG
AGAAAAGATCCTCCTGTAAATGAGGCTTATTTATATGCAACCCATCTTTTAGAAGTTGCCGAAAGAAAAGGAGTAAAAGT
AATTAACAAACCCTCATCGTTAAGAGCATGGAATGAAAAGCTAGGTGCTTTGAGATACAGCCACTTAATGGCACCTACAA
TAGTTGCGAGTAAGGTAAAAGATCTTATTAATTTTGCAAATATAAATAATGAAGTAGTCATAAAACCTCTTGGAGGAAAA
GGTGGACAAGGTGTTATAAGGATAAATAAAAATTCTCCAGGAATTAAATCAATCATTGAATTAATCACTTCTCAAGAAGA
ATTACCCGTTATGATGCAAAAATTTATTCCTAAAGTCATAGAGGGTGATAAAAGAATAATTATCGTAAACGGTGAAGCAA
TTGGATCTATAAATAGGATTCCTCAAGGAGGCGATTTTAGGAGTAATTTAGCGTTGGGAGGGAAGGCTGAACCCACATTA
TTAACAGAAAAAGAAAAAAGTATTTGCTCAGAGTTATCTCAACATTTCAAAGATGAAGGTTTGTTTTTTGTAGGTATTGA
TGTAATAAATGGAATGCTTAGCGAGATTAATGTAACCAGTCCAACAGGTTTAAGAGAAATAGAAAATTTATCTAATAAAA
ATGTTTCAGAAGAAGTTATTGAAAAGTTAATAGAAATTATTTAG

Upstream 100 bases:

>100_bases
TACCACAAATCTTTATAGATAATGAGGGTATCGGAGGCTGCGATGATCTCTACGCATTAGAAAATGAAAATAAATTAGAA
GCATTATTAAACTAGATCTT

Downstream 100 bases:

>100_bases
AAGTTTTAGCAAAAACTATTTGTTTTACTAAAGATTCAAATTTTAATCTAATCTCATCTATTTCTTTTTCTAAAACAGAT
CCTGAAACTATCCCTGAACC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKFLFVIDPIKNINPLKDSTAALMQASSKKNIEIWSCTPQDLEARGDEVWASSVKVEVNPWISFKENDCIPLAEFNCIWM
RKDPPVNEAYLYATHLLEVAERKGVKVINKPSSLRAWNEKLGALRYSHLMAPTIVASKVKDLINFANINNEVVIKPLGGK
GGQGVIRINKNSPGIKSIIELITSQEELPVMMQKFIPKVIEGDKRIIIVNGEAIGSINRIPQGGDFRSNLALGGKAEPTL
LTEKEKSICSELSQHFKDEGLFFVGIDVINGMLSEINVTSPTGLREIENLSNKNVSEEVIEKLIEII

Sequences:

>Translated_307_residues
MKFLFVIDPIKNINPLKDSTAALMQASSKKNIEIWSCTPQDLEARGDEVWASSVKVEVNPWISFKENDCIPLAEFNCIWM
RKDPPVNEAYLYATHLLEVAERKGVKVINKPSSLRAWNEKLGALRYSHLMAPTIVASKVKDLINFANINNEVVIKPLGGK
GGQGVIRINKNSPGIKSIIELITSQEELPVMMQKFIPKVIEGDKRIIIVNGEAIGSINRIPQGGDFRSNLALGGKAEPTL
LTEKEKSICSELSQHFKDEGLFFVGIDVINGMLSEINVTSPTGLREIENLSNKNVSEEVIEKLIEII
>Mature_307_residues
MKFLFVIDPIKNINPLKDSTAALMQASSKKNIEIWSCTPQDLEARGDEVWASSVKVEVNPWISFKENDCIPLAEFNCIWM
RKDPPVNEAYLYATHLLEVAERKGVKVINKPSSLRAWNEKLGALRYSHLMAPTIVASKVKDLINFANINNEVVIKPLGGK
GGQGVIRINKNSPGIKSIIELITSQEELPVMMQKFIPKVIEGDKRIIIVNGEAIGSINRIPQGGDFRSNLALGGKAEPTL
LTEKEKSICSELSQHFKDEGLFFVGIDVINGMLSEINVTSPTGLREIENLSNKNVSEEVIEKLIEII

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=293, Percent_Identity=39.9317406143345, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34091; Mature: 34091

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLFVIDPIKNINPLKDSTAALMQASSKKNIEIWSCTPQDLEARGDEVWASSVKVEVNP
CEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCEECCEEEEEECC
WISFKENDCIPLAEFNCIWMRKDPPVNEAYLYATHLLEVAERKGVKVINKPSSLRAWNEK
CEEECCCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHH
LGALRYSHLMAPTIVASKVKDLINFANINNEVVIKPLGGKGGQGVIRINKNSPGIKSIIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHH
LITSQEELPVMMQKFIPKVIEGDKRIIIVNGEAIGSINRIPQGGDFRSNLALGGKAEPTL
HHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCE
LTEKEKSICSELSQHFKDEGLFFVGIDVINGMLSEINVTSPTGLREIENLSNKNVSEEVI
ECHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHH
EKLIEII
HHHHHHC
>Mature Secondary Structure
MKFLFVIDPIKNINPLKDSTAALMQASSKKNIEIWSCTPQDLEARGDEVWASSVKVEVNP
CEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCEECCEEEEEECC
WISFKENDCIPLAEFNCIWMRKDPPVNEAYLYATHLLEVAERKGVKVINKPSSLRAWNEK
CEEECCCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHH
LGALRYSHLMAPTIVASKVKDLINFANINNEVVIKPLGGKGGQGVIRINKNSPGIKSIIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHH
LITSQEELPVMMQKFIPKVIEGDKRIIIVNGEAIGSINRIPQGGDFRSNLALGGKAEPTL
HHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCE
LTEKEKSICSELSQHFKDEGLFFVGIDVINGMLSEINVTSPTGLREIENLSNKNVSEEVI
ECHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHH
EKLIEII
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]