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The map label for this gene is menE [H]

Identifier: 78778562

GI number: 78778562

Start: 168212

End: 169426

Strand: Reverse

Name: menE [H]

Synonym: PMT9312_0176

Alternate gene names: 78778562

Gene position: 169426-168212 (Counterclockwise)

Preceding gene: 78778563

Following gene: 78778559

Centisome position: 9.91

GC content: 27.08

Gene sequence:

>1215_bases
ATGGAATCAATTATGAAAAATAAAATTCATATTATTGAAGTTGAAAATAATGAAACTCAATCTATAAAGAAAATTATTCA
GAAAATTAGAGAGAATAAAATAATTTATATAAGAAACAAATTTTATAAAAACGAAGAGGTGCTAGATACGATTCATAAAA
ATGGGCCAGCAATTATCTTAAACAGCAGTGGGAGCAGTGGTAAACCTAGAAAATGTATTCACCATTTAGATAATCTCAAA
TTATCTGCCGCTTCTTCAGGACAATGGCTAAAAGAGCAAGGATTTGAATTGCAAAACTGCTTAATTTTCAATACTTTACC
CCTTAACCATATAAGTGGATTAATGCCAATTTTTAGAAGTCAAACTTGGGGATGTAATTGTATTAATATTTCTCCGAATT
TAATAAAAAAAACTCGAGAACTTTTACTTTTTACAATTAAATATAAAAAAAACAAAAAGCACTTGATTACGTCATTAGTA
CCTACCCAATTAAACAGACTTTTATCTGAAAAAGATGGTATTAATTGGCTAAAAATTTTTGATTTAATTTGGGTAGGTGG
AGCTTCAATTACCCGGGAAACTGCAGAAAAATGTATACAAGAACAAATAAAATTAGCACCTTGTTACGGCTCAACTGAAA
CAGCAGCAATGGTTACGAGTTTAAAACCAAAAGAATTCTTAATGGGTTTTGAAAACGTTGGAGAAATACTACCTGATACA
AAAATAAGAATTAACAAACAAGGATTAATCGAGATAAAATCAGCCAGAATTGGAATCGAAATAATAGAATCTTCAAAAAC
TGAAAATTTCAAAAATAAAAATGGGTGGTGGCAAACTGGTGATTTAGGTGAAATTAATCAAATCAATAATTCTTTATATT
TAAAGTTTATTGGAAGAATTGATAACGCTTTTAATTCAGGAGGAGAAATCGTTTTCCCAGAAGTAATTGAATCTCGATTA
AATGATTTCATTATGAAAGAAAATATCCCAATTAATAAATTCAATATTTCAAAAGTACCCGACAAATTATGGGGAAATAA
AATTAGTATAATTGTTGAATTTAGAGAACTTACAAATCATAAAAATATTGAAAATTCATTAAATTTATTAAAAAACTTTT
CTCAAAGTTGGCCTAAACATGAAAAACCAGAAAAGTGGATTATTAAAAACAAAAATACAAGTACAGAAAAAATAAACTAT
CAATTTAAAAAATAA

Upstream 100 bases:

>100_bases
TATCATCTCTGCAGTTACAAGGACCAACACCAAAAGTTCCTGGTTTAGCAATGAATAAATTCCCTAACTCTTTCCTATTT
ACAAATGAAGCACAAAAGAT

Downstream 100 bases:

>100_bases
TAAATAAAAATTAGCACTCTTTTAAATTTGTACTTATATTTGAAGCCTCTATCCATCTTTCTAGCTGATCGGGAAGTTCT
ATTTTATTTCTTGAATCAAC

Product: putative O-succinylbenzoic acid--CoA ligase (OSB-CoA synthetase)

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MESIMKNKIHIIEVENNETQSIKKIIQKIRENKIIYIRNKFYKNEEVLDTIHKNGPAIILNSSGSSGKPRKCIHHLDNLK
LSAASSGQWLKEQGFELQNCLIFNTLPLNHISGLMPIFRSQTWGCNCINISPNLIKKTRELLLFTIKYKKNKKHLITSLV
PTQLNRLLSEKDGINWLKIFDLIWVGGASITRETAEKCIQEQIKLAPCYGSTETAAMVTSLKPKEFLMGFENVGEILPDT
KIRINKQGLIEIKSARIGIEIIESSKTENFKNKNGWWQTGDLGEINQINNSLYLKFIGRIDNAFNSGGEIVFPEVIESRL
NDFIMKENIPINKFNISKVPDKLWGNKISIIVEFRELTNHKNIENSLNLLKNFSQSWPKHEKPEKWIIKNKNTSTEKINY
QFKK

Sequences:

>Translated_404_residues
MESIMKNKIHIIEVENNETQSIKKIIQKIRENKIIYIRNKFYKNEEVLDTIHKNGPAIILNSSGSSGKPRKCIHHLDNLK
LSAASSGQWLKEQGFELQNCLIFNTLPLNHISGLMPIFRSQTWGCNCINISPNLIKKTRELLLFTIKYKKNKKHLITSLV
PTQLNRLLSEKDGINWLKIFDLIWVGGASITRETAEKCIQEQIKLAPCYGSTETAAMVTSLKPKEFLMGFENVGEILPDT
KIRINKQGLIEIKSARIGIEIIESSKTENFKNKNGWWQTGDLGEINQINNSLYLKFIGRIDNAFNSGGEIVFPEVIESRL
NDFIMKENIPINKFNISKVPDKLWGNKISIIVEFRELTNHKNIENSLNLLKNFSQSWPKHEKPEKWIIKNKNTSTEKINY
QFKK
>Mature_404_residues
MESIMKNKIHIIEVENNETQSIKKIIQKIRENKIIYIRNKFYKNEEVLDTIHKNGPAIILNSSGSSGKPRKCIHHLDNLK
LSAASSGQWLKEQGFELQNCLIFNTLPLNHISGLMPIFRSQTWGCNCINISPNLIKKTRELLLFTIKYKKNKKHLITSLV
PTQLNRLLSEKDGINWLKIFDLIWVGGASITRETAEKCIQEQIKLAPCYGSTETAAMVTSLKPKEFLMGFENVGEILPDT
KIRINKQGLIEIKSARIGIEIIESSKTENFKNKNGWWQTGDLGEINQINNSLYLKFIGRIDNAFNSGGEIVFPEVIESRL
NDFIMKENIPINKFNISKVPDKLWGNKISIIVEFRELTNHKNIENSLNLLKNFSQSWPKHEKPEKWIIKNKNTSTEKINY
QFKK

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=332, Percent_Identity=25, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32563687, Length=331, Percent_Identity=26.5861027190332, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=351, Percent_Identity=24.7863247863248, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 46535; Mature: 46535

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIMKNKIHIIEVENNETQSIKKIIQKIRENKIIYIRNKFYKNEEVLDTIHKNGPAIIL
CCCHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCEEEE
NSSGSSGKPRKCIHHLDNLKLSAASSGQWLKEQGFELQNCLIFNTLPLNHISGLMPIFRS
ECCCCCCCHHHHHHHHHCCEEECCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCC
QTWGCNCINISPNLIKKTRELLLFTIKYKKNKKHLITSLVPTQLNRLLSEKDGINWLKIF
CCCCCEEEECCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DLIWVGGASITRETAEKCIQEQIKLAPCYGSTETAAMVTSLKPKEFLMGFENVGEILPDT
HHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHCCCHHHHHHCHHHHHHHCCCC
KIRINKQGLIEIKSARIGIEIIESSKTENFKNKNGWWQTGDLGEINQINNSLYLKFIGRI
EEEECCCCCEEEECCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEHHHHHH
DNAFNSGGEIVFPEVIESRLNDFIMKENIPINKFNISKVPDKLWGNKISIIVEFRELTNH
HHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCEEEEEEEEHHHCCC
KNIENSLNLLKNFSQSWPKHEKPEKWIIKNKNTSTEKINYQFKK
CCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEEECC
>Mature Secondary Structure
MESIMKNKIHIIEVENNETQSIKKIIQKIRENKIIYIRNKFYKNEEVLDTIHKNGPAIIL
CCCHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCEEEE
NSSGSSGKPRKCIHHLDNLKLSAASSGQWLKEQGFELQNCLIFNTLPLNHISGLMPIFRS
ECCCCCCCHHHHHHHHHCCEEECCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCC
QTWGCNCINISPNLIKKTRELLLFTIKYKKNKKHLITSLVPTQLNRLLSEKDGINWLKIF
CCCCCEEEECCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DLIWVGGASITRETAEKCIQEQIKLAPCYGSTETAAMVTSLKPKEFLMGFENVGEILPDT
HHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHCCCHHHHHHCHHHHHHHCCCC
KIRINKQGLIEIKSARIGIEIIESSKTENFKNKNGWWQTGDLGEINQINNSLYLKFIGRI
EEEECCCCCEEEECCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEHHHHHH
DNAFNSGGEIVFPEVIESRLNDFIMKENIPINKFNISKVPDKLWGNKISIIVEFRELTNH
HHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCEEEEEEEEHHHCCC
KNIENSLNLLKNFSQSWPKHEKPEKWIIKNKNTSTEKINYQFKK
CCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA