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The map label for this gene is ndhF [H]

Identifier: 78778537

GI number: 78778537

Start: 145257

End: 147278

Strand: Direct

Name: ndhF [H]

Synonym: PMT9312_0151

Alternate gene names: 78778537

Gene position: 145257-147278 (Clockwise)

Preceding gene: 78778536

Following gene: 78778538

Centisome position: 8.5

GC content: 36.6

Gene sequence:

>2022_bases
ATGCCTCAAGCTTCAGAAATTGCCTGGTTAATTCCTGTTTTCCCACTTATTGGAGCAGTGCTTTCTGGTTTAGGACTTAT
AAGTATCAATAAGAAAATTAATAATTCTAGAGAAATAGTTTCTGTAGGTCTGATTTCTTTTGTTGGCATTTCTGCGGTAA
TTAGTTATAAAGCTCTGATTGAACAAGTGAATGGTTATCAATCTGTAGAAAAATTATTTGTATGGGCTAGTGCTGGCGAT
TTCACAATTCCGATGGGTTTTGTCCTTGACCCTTTGGGCAGTGTAATGCTTGCTCTAGTAACCACAATAACTTTGCTGGT
TATGATTTACTCTCATGGTTATATGGCGCATGATAAAGGTTATGTCAGATTTTTTACATATTTAGCTCTTTTTAGTAGTT
CAATGATGGGATTAATAATTAGTCCAAATTTGTTAGAAATTTACGTTTTTTGGGAATTAGTGGGAATGTGTTCATACTTA
TTGGTTGGTTTTTGGTACGACAGAGATGGTGCTGCCCACGCTGCACAAAAAGCATTCGTTGTTAATAGGGTGGGAGACTT
TGGCTTATTACTAGGTATTCTTGGTCTATTTTGGGCAACTAATAGTTTTGATTTTAATGAAATAGCTATTGGAATTTCTC
AATCAATATCTGACAATTCAATACCTATTTGGGCTGCTTTACTACTTTGTTTTTTGGTTTTTTTAGGTCCAATGGCCAAA
TCTGCACAGTTTCCTCTTCATGTATGGTTACCTGATGCAATGGAGGGGCCGACACCGATCTCTGCACTTATCCATGCAGC
TACGATGGTTGCCGCAGGGATATTTCTAGTGGCGAGGCTGCAACCTTTGTATTCAATATTCCCCTATATTCAGTTTATTA
TTGCTTTAGTTGGTACCATTACTTGTTTTTTAGGCGCTTCTATAGCTTTGACCCAAATGGATTTAAAAAAGGGGTTAGCG
TACAGTACTGTCTCCCAACTGGGTTATATGATGCTTGCGATGGGTTGTGGAGCTCCAGTAGCAGGAATATTCCATTTGGT
TACTCATGCTTGCTTCAAGGCAATGTTATTTTTGGGATCCGGTTCTGTAATACATGCTATGGAAGAAGTCGTTGGTCATC
AGCCTATATTGGCTCAAGATATGAGATTGATGGGCGGTCTAAGAAAAAAAATGCCATATACTTCCGCAACATTTCTAATA
GGTTGTGTGGCAATTAGTGGTATTCCCCCATTGGCAGGTTTTTGGAGTAAAGACGAGATACTAGGAAATGCATTTATATC
ATTTCCAGCTTTTTGGTTTGTAGGGCTTTTAACAGCTGGCATGACTGCATTTTATATGTTTAGGCTTTATTTCTTGACAT
TTGAAGGAGATTTCAGAGGGGAGAATAAAGAATTACAAAAGGAGCTTTTAATCGCTTCCAACGTAAATTTAGATGAGGAA
AATGTAGAAGAGCATGAAGAGCATGGCTCTATTCATGAGTCTCCCTGGTCAATGACATTTCCTTTGGTTTTTCTGGCTGT
GCCATCTTTAATTATAGGTTTTATGGGACTTCCATGGGATAGTAAATTTGCAAATTTACTAGATCCTGAAGAAGCAGAGA
TTGCTGCAAAAACCTTCGAGATAAAAGAATTTATGCCTTTAGCGATAGCTTCAGTTCTTATTGCATCAGCTGGGATCGTT
ATTGCCTATCAGGCATATTTCTTGAAAAAAATTAATTTGTCAGTTTTATTTGCCAAGAAGTTTCCTTCTATTAATCAATT
CTTGTCAAATAAATGGTATCTAGATGATATTAATGAAAAACTTTTTGTTAAGGGTAGTAGAAAACTTGCTAAAGAAGTTT
TAGAAGTTGATTCTAAGGTTGTTGATGGCGTTGTTAATCTTACTGGACTCGTAACTTTAGGCAGTGGAGAAGGTTTAAAA
TATTTTGAGACTGGTAGAGCTCAATTTTACGCTCTTATTATTTTTGGAGGAGTTATTCTATTAGTTGCTATATTTGGTTT
TCAATCTCCTCAAGTAACTTAA

Upstream 100 bases:

>100_bases
ATCAGTACATAAATATTGCTGTTAAAACATTTAATTCATCATTTTTGTCTGAATTTTTCAATTGACAGTTTAAAATCTAA
AATATAAGTTTTTTAAAAAT

Downstream 100 bases:

>100_bases
TTACAATTGTGTGTCTTCACTGTCCATTGGGATGAAAAAATACAGATAATTTCTAGACTTTATTTAAGAGAAACTTCTTA
TTAAATTGAGTAATTATTAT

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MPQASEIAWLIPVFPLIGAVLSGLGLISINKKINNSREIVSVGLISFVGISAVISYKALIEQVNGYQSVEKLFVWASAGD
FTIPMGFVLDPLGSVMLALVTTITLLVMIYSHGYMAHDKGYVRFFTYLALFSSSMMGLIISPNLLEIYVFWELVGMCSYL
LVGFWYDRDGAAHAAQKAFVVNRVGDFGLLLGILGLFWATNSFDFNEIAIGISQSISDNSIPIWAALLLCFLVFLGPMAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGIFLVARLQPLYSIFPYIQFIIALVGTITCFLGASIALTQMDLKKGLA
YSTVSQLGYMMLAMGCGAPVAGIFHLVTHACFKAMLFLGSGSVIHAMEEVVGHQPILAQDMRLMGGLRKKMPYTSATFLI
GCVAISGIPPLAGFWSKDEILGNAFISFPAFWFVGLLTAGMTAFYMFRLYFLTFEGDFRGENKELQKELLIASNVNLDEE
NVEEHEEHGSIHESPWSMTFPLVFLAVPSLIIGFMGLPWDSKFANLLDPEEAEIAAKTFEIKEFMPLAIASVLIASAGIV
IAYQAYFLKKINLSVLFAKKFPSINQFLSNKWYLDDINEKLFVKGSRKLAKEVLEVDSKVVDGVVNLTGLVTLGSGEGLK
YFETGRAQFYALIIFGGVILLVAIFGFQSPQVT

Sequences:

>Translated_673_residues
MPQASEIAWLIPVFPLIGAVLSGLGLISINKKINNSREIVSVGLISFVGISAVISYKALIEQVNGYQSVEKLFVWASAGD
FTIPMGFVLDPLGSVMLALVTTITLLVMIYSHGYMAHDKGYVRFFTYLALFSSSMMGLIISPNLLEIYVFWELVGMCSYL
LVGFWYDRDGAAHAAQKAFVVNRVGDFGLLLGILGLFWATNSFDFNEIAIGISQSISDNSIPIWAALLLCFLVFLGPMAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGIFLVARLQPLYSIFPYIQFIIALVGTITCFLGASIALTQMDLKKGLA
YSTVSQLGYMMLAMGCGAPVAGIFHLVTHACFKAMLFLGSGSVIHAMEEVVGHQPILAQDMRLMGGLRKKMPYTSATFLI
GCVAISGIPPLAGFWSKDEILGNAFISFPAFWFVGLLTAGMTAFYMFRLYFLTFEGDFRGENKELQKELLIASNVNLDEE
NVEEHEEHGSIHESPWSMTFPLVFLAVPSLIIGFMGLPWDSKFANLLDPEEAEIAAKTFEIKEFMPLAIASVLIASAGIV
IAYQAYFLKKINLSVLFAKKFPSINQFLSNKWYLDDINEKLFVKGSRKLAKEVLEVDSKVVDGVVNLTGLVTLGSGEGLK
YFETGRAQFYALIIFGGVILLVAIFGFQSPQVT
>Mature_672_residues
PQASEIAWLIPVFPLIGAVLSGLGLISINKKINNSREIVSVGLISFVGISAVISYKALIEQVNGYQSVEKLFVWASAGDF
TIPMGFVLDPLGSVMLALVTTITLLVMIYSHGYMAHDKGYVRFFTYLALFSSSMMGLIISPNLLEIYVFWELVGMCSYLL
VGFWYDRDGAAHAAQKAFVVNRVGDFGLLLGILGLFWATNSFDFNEIAIGISQSISDNSIPIWAALLLCFLVFLGPMAKS
AQFPLHVWLPDAMEGPTPISALIHAATMVAAGIFLVARLQPLYSIFPYIQFIIALVGTITCFLGASIALTQMDLKKGLAY
STVSQLGYMMLAMGCGAPVAGIFHLVTHACFKAMLFLGSGSVIHAMEEVVGHQPILAQDMRLMGGLRKKMPYTSATFLIG
CVAISGIPPLAGFWSKDEILGNAFISFPAFWFVGLLTAGMTAFYMFRLYFLTFEGDFRGENKELQKELLIASNVNLDEEN
VEEHEEHGSIHESPWSMTFPLVFLAVPSLIIGFMGLPWDSKFANLLDPEEAEIAAKTFEIKEFMPLAIASVLIASAGIVI
AYQAYFLKKINLSVLFAKKFPSINQFLSNKWYLDDINEKLFVKGSRKLAKEVLEVDSKVVDGVVNLTGLVTLGSGEGLKY
FETGRAQFYALIIFGGVILLVAIFGFQSPQVT

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=459, Percent_Identity=36.8191721132898, Blast_Score=268, Evalue=1e-71,
Organism=Escherichia coli, GI1788614, Length=648, Percent_Identity=37.1913580246914, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1788829, Length=400, Percent_Identity=30, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788827, Length=423, Percent_Identity=28.3687943262411, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788831, Length=361, Percent_Identity=30.7479224376731, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788613, Length=366, Percent_Identity=26.775956284153, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI2367154, Length=402, Percent_Identity=25.8706467661692, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 73715; Mature: 73584

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQASEIAWLIPVFPLIGAVLSGLGLISINKKINNSREIVSVGLISFVGISAVISYKALI
CCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQVNGYQSVEKLFVWASAGDFTIPMGFVLDPLGSVMLALVTTITLLVMIYSHGYMAHDKG
HHHCCHHHHHHHEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
YVRFFTYLALFSSSMMGLIISPNLLEIYVFWELVGMCSYLLVGFWYDRDGAAHAAQKAFV
HHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
VNRVGDFGLLLGILGLFWATNSFDFNEIAIGISQSISDNSIPIWAALLLCFLVFLGPMAK
HHHCCHHHHHHHHHHHHHCCCCCCHHHHEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGIFLVARLQPLYSIFPYIQFIIALVGTI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCFLGASIALTQMDLKKGLAYSTVSQLGYMMLAMGCGAPVAGIFHLVTHACFKAMLFLGS
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
GSVIHAMEEVVGHQPILAQDMRLMGGLRKKMPYTSATFLIGCVAISGIPPLAGFWSKDEI
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHH
LGNAFISFPAFWFVGLLTAGMTAFYMFRLYFLTFEGDFRGENKELQKELLIASNVNLDEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCCCCHH
NVEEHEEHGSIHESPWSMTFPLVFLAVPSLIIGFMGLPWDSKFANLLDPEEAEIAAKTFE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHH
IKEFMPLAIASVLIASAGIVIAYQAYFLKKINLSVLFAKKFPSINQFLSNKWYLDDINEK
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHCCCCEEECCCCC
LFVKGSRKLAKEVLEVDSKVVDGVVNLTGLVTLGSGEGLKYFETGRAQFYALIIFGGVIL
EEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHH
LVAIFGFQSPQVT
HHHHHCCCCCCCC
>Mature Secondary Structure 
PQASEIAWLIPVFPLIGAVLSGLGLISINKKINNSREIVSVGLISFVGISAVISYKALI
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQVNGYQSVEKLFVWASAGDFTIPMGFVLDPLGSVMLALVTTITLLVMIYSHGYMAHDKG
HHHCCHHHHHHHEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
YVRFFTYLALFSSSMMGLIISPNLLEIYVFWELVGMCSYLLVGFWYDRDGAAHAAQKAFV
HHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
VNRVGDFGLLLGILGLFWATNSFDFNEIAIGISQSISDNSIPIWAALLLCFLVFLGPMAK
HHHCCHHHHHHHHHHHHHCCCCCCHHHHEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGIFLVARLQPLYSIFPYIQFIIALVGTI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCFLGASIALTQMDLKKGLAYSTVSQLGYMMLAMGCGAPVAGIFHLVTHACFKAMLFLGS
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
GSVIHAMEEVVGHQPILAQDMRLMGGLRKKMPYTSATFLIGCVAISGIPPLAGFWSKDEI
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHH
LGNAFISFPAFWFVGLLTAGMTAFYMFRLYFLTFEGDFRGENKELQKELLIASNVNLDEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCCCCHH
NVEEHEEHGSIHESPWSMTFPLVFLAVPSLIIGFMGLPWDSKFANLLDPEEAEIAAKTFE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHH
IKEFMPLAIASVLIASAGIVIAYQAYFLKKINLSVLFAKKFPSINQFLSNKWYLDDINEK
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHCCCCEEECCCCC
LFVKGSRKLAKEVLEVDSKVVDGVVNLTGLVTLGSGEGLKYFETGRAQFYALIIFGGVIL
EEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHH
LVAIFGFQSPQVT
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]