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The map label for this gene is nadB [H]

Identifier: 78778489

GI number: 78778489

Start: 104919

End: 106586

Strand: Direct

Name: nadB [H]

Synonym: PMT9312_0104

Alternate gene names: 78778489

Gene position: 104919-106586 (Clockwise)

Preceding gene: 78778488

Following gene: 78778491

Centisome position: 6.14

GC content: 35.25

Gene sequence:

>1668_bases
ATGTTAAGGCCTCCATTTTCGCACGAACCGATTCCATTAAATAATTGGGATGTCATTGTTATAGGTGCTGGAGCTGCAGG
ACTTATGACTTGTCTTGAGTTACCTGCAAATTTAAAAGTGCTACTTTTAAATAGAAACACAAGCAAGGTATCTTCTAGTA
GATGGGCTCAAGGAGGAATAGCATCTGTTGTTAGACAGGACGATTCATTCGATCTTCATGCTGATGATACTTTAAAAGCA
GGAGATGGACTATGTGATTTTCAAGCGGTAGAAATGCTGGTTAAAGAAGCCCCTGGTTGTGTTGACAGGTTGCAGAATTT
AGGGATGATTTTTGATCAAAGTTTTGATCAACTAGCTACTACTTTAGAAGCGGCCCATTCTCGCAGAAGAGTCTTGCATG
TTAAAGATCGTACTGGAAGAGCATTAGTTGAAGTTCTAGAAGATCATATTGAAAATAAAAAAAATATTCTTCACTGTAGG
GGTGTAAGAGTAACTGAACTTCTTATTGAAAATGAAGAATGCAAGGGAGTTCAGGTTCTTGATGGAGCTAATTTGTATTG
GATTAAATCGAGAGCTGTTGTTTTAGCTACAGGTGGAGGTGGGCATTTATTTACTAATACAACCAATCCCGCGCAATCCT
CTGGAGAAGGGATCGCTCTTGCATGGAAAGCAGGTGCTGCTATTGAAGATTTAGAGTTTGTTCAATTTCATCCAACCGCT
TTAAAATTCTATGGCGCCCCTTGTTTCTTAATATCTGAGGCACTTAGAGGAGAAGGCGCAATATTAATCGATAAAAATGG
TGAAAGCCCAGTTAAAAATCTTAAAAATCGTGATTTAGCTACTAGAGATCAGGTAAGTAGAGCAATTATGAAGAATATGT
ATGACAATAATGTGGATCATGTTGGCTTAGATCTTCGTTACATTGATCCAGAAAAACTTGTAGAGCGATTTCCCACAATT
TTAAGTAGATGTCAGGATTATGGCGTTAATCCTTTAAATGAGATTATTCCTGTCGCTCCAGCAGCTCATTATTGGATGGG
AGGCGTTAAAACTGATTTAAGTGCATCTTCTACAAGAAAAGGATTATATGCTGTAGGAGAAGTTGCTTCTACAGGTGTGC
ATGGTGCGAATAGATTGGCAAGTAATTCACTAATGGAATGCCTCGTTTTTGCGAGAAAACTGTCTTCAATTGTTCTGAGT
GATCCTCCTAAATTCGCAAAATTTGATAGATCATTCCAAGAGTTCGATATTAAAGACCCGAAAGAAGATCAAATTTCTCA
AATTGCAGAAAAAATTGATGAATTAAGAAAACTATGTTGGTCAAATCTAGGTGTATCTCGAAATAAAGTAAATATGAGTA
AATTTCTCGATACTATTCAAGATGATATGGCTCAATTACAAAAAAATTCCTTACTAAATAGTCTTGAAAAAATAAATTTT
GATCAAGAAATTAGACTTAGTGAACCTAATAGAAGGGCACTTAATCTTTTACTTGATTTAAAGAATAGACAAATAACAAC
TATAACTTTATTAAAAGCTTGTCTTTTTAGAGAAGAAAGCAGAGGAGGTCATTATAGAGATGATTTTCCAAATAAAGATA
AAAATTGGGTATGCCATACTAGACAACTGTTAGATAAAGAAATTCATAAAAGATTTATTAAAAATTAG

Upstream 100 bases:

>100_bases
CACTCTTAGTTTCTAGTTATGTAATTCAAACTACATTAATTGTTGATGGAATTTTTATTTTTTTAGCAATTCTGCAACCA
AATTTTAGTGGATTGGTTTA

Downstream 100 bases:

>100_bases
AATCTCCTTGATAGCCAGTCGTTATCGCTAAATCATTCAGGTCGAGAGTACCGCTAATAATTTCTCCATTTATTTCCCAA
GAAGGAAATCCACTAATTCC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MLRPPFSHEPIPLNNWDVIVIGAGAAGLMTCLELPANLKVLLLNRNTSKVSSSRWAQGGIASVVRQDDSFDLHADDTLKA
GDGLCDFQAVEMLVKEAPGCVDRLQNLGMIFDQSFDQLATTLEAAHSRRRVLHVKDRTGRALVEVLEDHIENKKNILHCR
GVRVTELLIENEECKGVQVLDGANLYWIKSRAVVLATGGGGHLFTNTTNPAQSSGEGIALAWKAGAAIEDLEFVQFHPTA
LKFYGAPCFLISEALRGEGAILIDKNGESPVKNLKNRDLATRDQVSRAIMKNMYDNNVDHVGLDLRYIDPEKLVERFPTI
LSRCQDYGVNPLNEIIPVAPAAHYWMGGVKTDLSASSTRKGLYAVGEVASTGVHGANRLASNSLMECLVFARKLSSIVLS
DPPKFAKFDRSFQEFDIKDPKEDQISQIAEKIDELRKLCWSNLGVSRNKVNMSKFLDTIQDDMAQLQKNSLLNSLEKINF
DQEIRLSEPNRRALNLLLDLKNRQITTITLLKACLFREESRGGHYRDDFPNKDKNWVCHTRQLLDKEIHKRFIKN

Sequences:

>Translated_555_residues
MLRPPFSHEPIPLNNWDVIVIGAGAAGLMTCLELPANLKVLLLNRNTSKVSSSRWAQGGIASVVRQDDSFDLHADDTLKA
GDGLCDFQAVEMLVKEAPGCVDRLQNLGMIFDQSFDQLATTLEAAHSRRRVLHVKDRTGRALVEVLEDHIENKKNILHCR
GVRVTELLIENEECKGVQVLDGANLYWIKSRAVVLATGGGGHLFTNTTNPAQSSGEGIALAWKAGAAIEDLEFVQFHPTA
LKFYGAPCFLISEALRGEGAILIDKNGESPVKNLKNRDLATRDQVSRAIMKNMYDNNVDHVGLDLRYIDPEKLVERFPTI
LSRCQDYGVNPLNEIIPVAPAAHYWMGGVKTDLSASSTRKGLYAVGEVASTGVHGANRLASNSLMECLVFARKLSSIVLS
DPPKFAKFDRSFQEFDIKDPKEDQISQIAEKIDELRKLCWSNLGVSRNKVNMSKFLDTIQDDMAQLQKNSLLNSLEKINF
DQEIRLSEPNRRALNLLLDLKNRQITTITLLKACLFREESRGGHYRDDFPNKDKNWVCHTRQLLDKEIHKRFIKN
>Mature_555_residues
MLRPPFSHEPIPLNNWDVIVIGAGAAGLMTCLELPANLKVLLLNRNTSKVSSSRWAQGGIASVVRQDDSFDLHADDTLKA
GDGLCDFQAVEMLVKEAPGCVDRLQNLGMIFDQSFDQLATTLEAAHSRRRVLHVKDRTGRALVEVLEDHIENKKNILHCR
GVRVTELLIENEECKGVQVLDGANLYWIKSRAVVLATGGGGHLFTNTTNPAQSSGEGIALAWKAGAAIEDLEFVQFHPTA
LKFYGAPCFLISEALRGEGAILIDKNGESPVKNLKNRDLATRDQVSRAIMKNMYDNNVDHVGLDLRYIDPEKLVERFPTI
LSRCQDYGVNPLNEIIPVAPAAHYWMGGVKTDLSASSTRKGLYAVGEVASTGVHGANRLASNSLMECLVFARKLSSIVLS
DPPKFAKFDRSFQEFDIKDPKEDQISQIAEKIDELRKLCWSNLGVSRNKVNMSKFLDTIQDDMAQLQKNSLLNSLEKINF
DQEIRLSEPNRRALNLLLDLKNRQITTITLLKACLFREESRGGHYRDDFPNKDKNWVCHTRQLLDKEIHKRFIKN

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=508, Percent_Identity=31.6929133858268, Blast_Score=212, Evalue=7e-55,
Organism=Escherichia coli, GI1788928, Length=540, Percent_Identity=35.9259259259259, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1790597, Length=548, Percent_Identity=29.5620437956204, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1786942, Length=586, Percent_Identity=29.8634812286689, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17550100, Length=536, Percent_Identity=31.3432835820896, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17505833, Length=543, Percent_Identity=30.3867403314917, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI71986328, Length=418, Percent_Identity=28.9473684210526, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322416, Length=582, Percent_Identity=30.7560137457045, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6322701, Length=548, Percent_Identity=29.3795620437956, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322511, Length=462, Percent_Identity=27.2727272727273, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320788, Length=472, Percent_Identity=27.5423728813559, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17137288, Length=573, Percent_Identity=30.8900523560209, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24655642, Length=573, Percent_Identity=30.8900523560209, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24655647, Length=573, Percent_Identity=30.8900523560209, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24663005, Length=523, Percent_Identity=29.0630975143403, Blast_Score=180, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 62083; Mature: 62083

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPPFSHEPIPLNNWDVIVIGAGAAGLMTCLELPANLKVLLLNRNTSKVSSSRWAQGGI
CCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCH
ASVVRQDDSFDLHADDTLKAGDGLCDFQAVEMLVKEAPGCVDRLQNLGMIFDQSFDQLAT
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHCCHHHHHH
TLEAAHSRRRVLHVKDRTGRALVEVLEDHIENKKNILHCRGVRVTELLIENEECKGVQVL
HHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCEEEHHEECCCCCCCEEEE
DGANLYWIKSRAVVLATGGGGHLFTNTTNPAQSSGEGIALAWKAGAAIEDLEFVQFHPTA
CCCCEEEEECCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCHHCCHHEEECCHH
LKFYGAPCFLISEALRGEGAILIDKNGESPVKNLKNRDLATRDQVSRAIMKNMYDNNVDH
HHHCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCE
VGLDLRYIDPEKLVERFPTILSRCQDYGVNPLNEIIPVAPAAHYWMGGVKTDLSASSTRK
EEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHCCCCCCCCCCCHHCC
GLYAVGEVASTGVHGANRLASNSLMECLVFARKLSSIVLSDPPKFAKFDRSFQEFDIKDP
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHCCCCCC
KEDQISQIAEKIDELRKLCWSNLGVSRNKVNMSKFLDTIQDDMAQLQKNSLLNSLEKINF
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DQEIRLSEPNRRALNLLLDLKNRQITTITLLKACLFREESRGGHYRDDFPNKDKNWVCHT
CCCEECCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
RQLLDKEIHKRFIKN
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MLRPPFSHEPIPLNNWDVIVIGAGAAGLMTCLELPANLKVLLLNRNTSKVSSSRWAQGGI
CCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCH
ASVVRQDDSFDLHADDTLKAGDGLCDFQAVEMLVKEAPGCVDRLQNLGMIFDQSFDQLAT
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHCCHHHHHH
TLEAAHSRRRVLHVKDRTGRALVEVLEDHIENKKNILHCRGVRVTELLIENEECKGVQVL
HHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCEEEHHEECCCCCCCEEEE
DGANLYWIKSRAVVLATGGGGHLFTNTTNPAQSSGEGIALAWKAGAAIEDLEFVQFHPTA
CCCCEEEEECCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCHHCCHHEEECCHH
LKFYGAPCFLISEALRGEGAILIDKNGESPVKNLKNRDLATRDQVSRAIMKNMYDNNVDH
HHHCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCE
VGLDLRYIDPEKLVERFPTILSRCQDYGVNPLNEIIPVAPAAHYWMGGVKTDLSASSTRK
EEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHCCCCCCCCCCCHHCC
GLYAVGEVASTGVHGANRLASNSLMECLVFARKLSSIVLSDPPKFAKFDRSFQEFDIKDP
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHCCCCCC
KEDQISQIAEKIDELRKLCWSNLGVSRNKVNMSKFLDTIQDDMAQLQKNSLLNSLEKINF
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DQEIRLSEPNRRALNLLLDLKNRQITTITLLKACLFREESRGGHYRDDFPNKDKNWVCHT
CCCEECCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
RQLLDKEIHKRFIKN
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]