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The map label for this gene is purF [H]

Identifier: 78778389

GI number: 78778389

Start: 4459

End: 5919

Strand: Direct

Name: purF [H]

Synonym: PMT9312_0004

Alternate gene names: 78778389

Gene position: 4459-5919 (Clockwise)

Preceding gene: 78778388

Following gene: 78778393

Centisome position: 0.26

GC content: 32.31

Gene sequence:

>1461_bases
ATGTGCGGAATAGTTGGAATCGTTTCTTCAGATGATGTAAATCAACAAATTTACGATAGCCTTTTGCTTCTACAGCATAG
AGGTCAAGATTCAACTGGTATAGCTACAATGGAAAATACTATTTTCCATATACATAAGGCTAAAGGTCAGGTGAATACTG
CTTATAGAACCAGAGATATGAGGAATTTAATCGGAAAAATTGGATTAGGTCATGTTAGGTATGCAACAAAGGGATCAGCA
GAAAGTGTAGAAGAAGCTCAACCTTTTTACGTTAATGCTCCTTATGGAATTGTTTTGATACATAATGGTAATTTGACGAA
TACCAGAGATTTAGAAAAACAATTATTTAATATTGATAAGCGGCATACAAATTCTTCAAGTGATACTGAAATGCTATTAA
ATGTACTTGCTACAGAATTGCAAGAACAAATTCATAATCAAGAATTAGAACCTGATATTATTTTTGATGCTGTAAATTCT
TTACATAAAAGAATTCAGGGATCATATGCTTCAATTGCATTACTTTCAGGACATGGTTTATTAGCATTCAGAGATCCTTT
TGGTATTAGACCTTTAGTCATAGGCAAAAGACTTTCATCAAAGACAAAAAAAGAAGAGTGGATGGTAGCTAGCGAATCTC
TAGTGCTTGAGAATAACGATTATCAAGTAGTGAGAGATGTAGATCCTGGAGAAGCTATTTTTATAAATCTTAATGGCGAA
TTTTTTTCTAAGCAATGTTCCGAAAATCCAATCTTATGTCCTTGTGCTTTTGAATATGTTTATTTGGCTAGGCCAGATTC
AATTATGAATGGAATTTCCGTTTATAAAGCTCGTTTAAAAATGGGAGATTATTTGTCTGAAACTATAAAAGAATCAATTA
ATACTGGAGATGTTGATGTTGTTATGCCTATTCCGGATTCTTCTCGACCTGCAGCTATGCAAGTTGCAAGACAGTTAGGT
ATAGAGTACAGAGAAGGTTTTTTTAAAAATAGATATGTTGGAAGAACATTCATAATGCCTGGTCAGCAGAAACGCAAGAA
ATCTGTAAGACAAAAATTAAATGCTATGAGTACAGAGTTTAAAAATAAAAATGTATTAATTGTTGATGACTCAATAGTGA
GAGGTACTACTTCAAAAGAAATTGTCCAGATGGCTAAAGATGCAGGAGCAAATAAAGTTTTTTTTACATCAGCAGCACCT
CCTGTGCGTTTTCCTCACGTTTATGGAATTAATATGCCTAATAGATGTGAATTAATAGCTCATGATAGAACAATAAGTGA
AATTGCTAATCAACTTGAAATTGATAACCTCGTTTATCAAAGTGTTGAAAATTTACGCAAATCTATAATAAGCGAATCTC
CTATTGAAGATTTAGAGATGAGTTGCTTTACTGGTATTTATGTAACAGGAAAAGTAAATCAAGAATATTTAAATTGGGTT
GAAAATGAATATAAATCTTAG

Upstream 100 bases:

>100_bases
GTGTTGAGGAATTAACCGAAAAATTTAATAATAGTATTTCAGGTTACTTTTAAATATGAAATATACTTCTCAACTATCAA
AATTTTTCAGTAATTAAGTT

Downstream 100 bases:

>100_bases
TCAAGAAAGTTTTCGAGTTGAAAACAATTTTCAATATATTCCTCATCGTCTAATTTTAAAAAGTCAATTAAAAAATTTGT
TTTCTTAGATTTAAAATTCA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MCGIVGIVSSDDVNQQIYDSLLLLQHRGQDSTGIATMENTIFHIHKAKGQVNTAYRTRDMRNLIGKIGLGHVRYATKGSA
ESVEEAQPFYVNAPYGIVLIHNGNLTNTRDLEKQLFNIDKRHTNSSSDTEMLLNVLATELQEQIHNQELEPDIIFDAVNS
LHKRIQGSYASIALLSGHGLLAFRDPFGIRPLVIGKRLSSKTKKEEWMVASESLVLENNDYQVVRDVDPGEAIFINLNGE
FFSKQCSENPILCPCAFEYVYLARPDSIMNGISVYKARLKMGDYLSETIKESINTGDVDVVMPIPDSSRPAAMQVARQLG
IEYREGFFKNRYVGRTFIMPGQQKRKKSVRQKLNAMSTEFKNKNVLIVDDSIVRGTTSKEIVQMAKDAGANKVFFTSAAP
PVRFPHVYGINMPNRCELIAHDRTISEIANQLEIDNLVYQSVENLRKSIISESPIEDLEMSCFTGIYVTGKVNQEYLNWV
ENEYKS

Sequences:

>Translated_486_residues
MCGIVGIVSSDDVNQQIYDSLLLLQHRGQDSTGIATMENTIFHIHKAKGQVNTAYRTRDMRNLIGKIGLGHVRYATKGSA
ESVEEAQPFYVNAPYGIVLIHNGNLTNTRDLEKQLFNIDKRHTNSSSDTEMLLNVLATELQEQIHNQELEPDIIFDAVNS
LHKRIQGSYASIALLSGHGLLAFRDPFGIRPLVIGKRLSSKTKKEEWMVASESLVLENNDYQVVRDVDPGEAIFINLNGE
FFSKQCSENPILCPCAFEYVYLARPDSIMNGISVYKARLKMGDYLSETIKESINTGDVDVVMPIPDSSRPAAMQVARQLG
IEYREGFFKNRYVGRTFIMPGQQKRKKSVRQKLNAMSTEFKNKNVLIVDDSIVRGTTSKEIVQMAKDAGANKVFFTSAAP
PVRFPHVYGINMPNRCELIAHDRTISEIANQLEIDNLVYQSVENLRKSIISESPIEDLEMSCFTGIYVTGKVNQEYLNWV
ENEYKS
>Mature_486_residues
MCGIVGIVSSDDVNQQIYDSLLLLQHRGQDSTGIATMENTIFHIHKAKGQVNTAYRTRDMRNLIGKIGLGHVRYATKGSA
ESVEEAQPFYVNAPYGIVLIHNGNLTNTRDLEKQLFNIDKRHTNSSSDTEMLLNVLATELQEQIHNQELEPDIIFDAVNS
LHKRIQGSYASIALLSGHGLLAFRDPFGIRPLVIGKRLSSKTKKEEWMVASESLVLENNDYQVVRDVDPGEAIFINLNGE
FFSKQCSENPILCPCAFEYVYLARPDSIMNGISVYKARLKMGDYLSETIKESINTGDVDVVMPIPDSSRPAAMQVARQLG
IEYREGFFKNRYVGRTFIMPGQQKRKKSVRQKLNAMSTEFKNKNVLIVDDSIVRGTTSKEIVQMAKDAGANKVFFTSAAP
PVRFPHVYGINMPNRCELIAHDRTISEIANQLEIDNLVYQSVENLRKSIISESPIEDLEMSCFTGIYVTGKVNQEYLNWV
ENEYKS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=475, Percent_Identity=37.4736842105263, Blast_Score=269, Evalue=5e-72,
Organism=Escherichia coli, GI1788651, Length=483, Percent_Identity=53.6231884057971, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1790167, Length=225, Percent_Identity=29.7777777777778, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554892, Length=486, Percent_Identity=32.9218106995885, Blast_Score=228, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6323958, Length=495, Percent_Identity=46.6666666666667, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24659598, Length=507, Percent_Identity=34.5167652859961, Blast_Score=258, Evalue=9e-69,
Organism=Drosophila melanogaster, GI28573187, Length=507, Percent_Identity=33.9250493096647, Blast_Score=255, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24659604, Length=482, Percent_Identity=35.2697095435685, Blast_Score=252, Evalue=4e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54734; Mature: 54734

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVSSDDVNQQIYDSLLLLQHRGQDSTGIATMENTIFHIHKAKGQVNTAYRTRDM
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHH
RNLIGKIGLGHVRYATKGSAESVEEAQPFYVNAPYGIVLIHNGNLTNTRDLEKQLFNIDK
HHHHHHCCCCEEEEECCCCHHHHHHCCCEEEECCEEEEEEECCCCCCHHHHHHHHHCCHH
RHTNSSSDTEMLLNVLATELQEQIHNQELEPDIIFDAVNSLHKRIQGSYASIALLSGHGL
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCE
LAFRDPFGIRPLVIGKRLSSKTKKEEWMVASESLVLENNDYQVVRDVDPGEAIFINLNGE
EEECCCCCCCHHEECCHHHCCCHHHHHEEECCEEEEECCCEEEEEECCCCCEEEEEECCH
FFSKQCSENPILCPCAFEYVYLARPDSIMNGISVYKARLKMGDYLSETIKESINTGDVDV
HHHHHCCCCCEEEEEHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VMPIPDSSRPAAMQVARQLGIEYREGFFKNRYVGRTFIMPGQQKRKKSVRQKLNAMSTEF
EEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
KNKNVLIVDDSIVRGTTSKEIVQMAKDAGANKVFFTSAAPPVRFPHVYGINMPNRCELIA
CCCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCEEEEE
HDRTISEIANQLEIDNLVYQSVENLRKSIISESPIEDLEMSCFTGIYVTGKVNQEYLNWV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEEECCCHHHHHHH
ENEYKS
HHHHCC
>Mature Secondary Structure
MCGIVGIVSSDDVNQQIYDSLLLLQHRGQDSTGIATMENTIFHIHKAKGQVNTAYRTRDM
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHH
RNLIGKIGLGHVRYATKGSAESVEEAQPFYVNAPYGIVLIHNGNLTNTRDLEKQLFNIDK
HHHHHHCCCCEEEEECCCCHHHHHHCCCEEEECCEEEEEEECCCCCCHHHHHHHHHCCHH
RHTNSSSDTEMLLNVLATELQEQIHNQELEPDIIFDAVNSLHKRIQGSYASIALLSGHGL
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCE
LAFRDPFGIRPLVIGKRLSSKTKKEEWMVASESLVLENNDYQVVRDVDPGEAIFINLNGE
EEECCCCCCCHHEECCHHHCCCHHHHHEEECCEEEEECCCEEEEEECCCCCEEEEEECCH
FFSKQCSENPILCPCAFEYVYLARPDSIMNGISVYKARLKMGDYLSETIKESINTGDVDV
HHHHHCCCCCEEEEEHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VMPIPDSSRPAAMQVARQLGIEYREGFFKNRYVGRTFIMPGQQKRKKSVRQKLNAMSTEF
EEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
KNKNVLIVDDSIVRGTTSKEIVQMAKDAGANKVFFTSAAPPVRFPHVYGINMPNRCELIA
CCCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCEEEEE
HDRTISEIANQLEIDNLVYQSVENLRKSIISESPIEDLEMSCFTGIYVTGKVNQEYLNWV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEEECCCHHHHHHH
ENEYKS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10873488; 11248100 [H]