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The map label for this gene is dnaN [H]

Identifier: 78778386

GI number: 78778386

Start: 205

End: 1362

Strand: Direct

Name: dnaN [H]

Synonym: PMT9312_0001

Alternate gene names: 78778386

Gene position: 205-1362 (Clockwise)

Preceding gene: NA

Following gene: 78778387

Centisome position: 0.01

GC content: 28.93

Gene sequence:

>1158_bases
ATGGAAATTATTTGTAATCAAAATGAGTTAAACAATGCTATACAACTAGTAAGTAAAGCAGTTGCTTCACGACCAACTCA
TCCAATTCTTGCAAATATACTTTTAACAGCTGACCAAGGAACTAATAAAATTAGTTTGACAGGATTTGATCTTAATTTAG
GAATTCAAACTTCTTTTGATGGAACTGTTAAAAATAGCGGTGCTATTACGATACCTTCAAAACTTTTATCTGAAATAGTA
AACAAATTACCTAATGAAAGTCCTGTTTCATTAGAAGTTGATGAAAATTTAGATAATATTTTAATAAAAAGTGATAGAGG
TTCTTTTAATCTTAAAGGCATACCCTCTGATGATTATCCTAATTTACCATTTGTTGAAAGTGGTACTTCTTTAAATATTG
ACCCTAGTTCTTTCTTGAAAGCTTTAAAAGCTACTATATTTGCTACTAGTAATGATGATTCCAAGCAATTACTTACGGGT
GTCAATTTTACCTTTAAACAAAATTATTTAGAGTCTGCCTCTACAGATGGTCATAGATTAGCGGTTGCGTTAATTGGTAA
TGAAGAACATATTGAAAATAAAGATAAATCACCTTCAATTGAAGATGATTTATCAGTAACTATTCCAACAAGATCATTAA
GAGAAATTGAAAAATTAGTATCTTTGAGAAGCTCAGAAAATTCAATAAAGCTATTCTACGATAAAGGTCAGGTAGTTTTT
ATTTCTTCTAATCAAATAATTACAACAAGAACTCTTGAAGGTGCCTATCCTAATTATTCGCAATTAATTCCTGATACTTT
TTCTAAGATTTTTAATTTTAATACTAAAAAATTAATTGATGCATTAGAAAGAATTGCTGTTTTAGCTGATCAGCAAAGTA
GTGTTGTGAAAATTAAATTGGATAATACAGATTTAGCTTCAATAAGTGCAGATGCACAAGATATTGGAAATGCAAATGAA
TCAATACCTGTTTCTTACTCTGGAGAAGATTTTGATATTGCATTTAACGTTAGATATCTTTTAGAAGGTTTAAAAGTTAT
TGCTTCAAAAAATGTACTATTAAAATGTAATCTTGCTACTACTCCAGCAGTTTTTGTACCAGAAGATAATCTTAATTCTT
TTACTTATCTAGTCATGCCTGTTCAGGTTCGTTCTTAA

Upstream 100 bases:

>100_bases
TATTTTATTTATTTATATTAGAAAAGAGAACAATATAAGTTATTGAATTTAATTTACGAAAAAAGTATATTGTGTATGTG
TTAAAAACTTCCAAATTCCG

Downstream 100 bases:

>100_bases
CTTGAAATTACCTAAAGAAATTTTATTAAGTGAATTACTAAATTATAGTGTTAAGGGTAGTATGTCACTTAATTATGGAA
ATGGTGAAAATGTTTGGATG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MEIICNQNELNNAIQLVSKAVASRPTHPILANILLTADQGTNKISLTGFDLNLGIQTSFDGTVKNSGAITIPSKLLSEIV
NKLPNESPVSLEVDENLDNILIKSDRGSFNLKGIPSDDYPNLPFVESGTSLNIDPSSFLKALKATIFATSNDDSKQLLTG
VNFTFKQNYLESASTDGHRLAVALIGNEEHIENKDKSPSIEDDLSVTIPTRSLREIEKLVSLRSSENSIKLFYDKGQVVF
ISSNQIITTRTLEGAYPNYSQLIPDTFSKIFNFNTKKLIDALERIAVLADQQSSVVKIKLDNTDLASISADAQDIGNANE
SIPVSYSGEDFDIAFNVRYLLEGLKVIASKNVLLKCNLATTPAVFVPEDNLNSFTYLVMPVQVRS

Sequences:

>Translated_385_residues
MEIICNQNELNNAIQLVSKAVASRPTHPILANILLTADQGTNKISLTGFDLNLGIQTSFDGTVKNSGAITIPSKLLSEIV
NKLPNESPVSLEVDENLDNILIKSDRGSFNLKGIPSDDYPNLPFVESGTSLNIDPSSFLKALKATIFATSNDDSKQLLTG
VNFTFKQNYLESASTDGHRLAVALIGNEEHIENKDKSPSIEDDLSVTIPTRSLREIEKLVSLRSSENSIKLFYDKGQVVF
ISSNQIITTRTLEGAYPNYSQLIPDTFSKIFNFNTKKLIDALERIAVLADQQSSVVKIKLDNTDLASISADAQDIGNANE
SIPVSYSGEDFDIAFNVRYLLEGLKVIASKNVLLKCNLATTPAVFVPEDNLNSFTYLVMPVQVRS
>Mature_385_residues
MEIICNQNELNNAIQLVSKAVASRPTHPILANILLTADQGTNKISLTGFDLNLGIQTSFDGTVKNSGAITIPSKLLSEIV
NKLPNESPVSLEVDENLDNILIKSDRGSFNLKGIPSDDYPNLPFVESGTSLNIDPSSFLKALKATIFATSNDDSKQLLTG
VNFTFKQNYLESASTDGHRLAVALIGNEEHIENKDKSPSIEDDLSVTIPTRSLREIEKLVSLRSSENSIKLFYDKGQVVF
ISSNQIITTRTLEGAYPNYSQLIPDTFSKIFNFNTKKLIDALERIAVLADQQSSVVKIKLDNTDLASISADAQDIGNANE
SIPVSYSGEDFDIAFNVRYLLEGLKVIASKNVLLKCNLATTPAVFVPEDNLNSFTYLVMPVQVRS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=384, Percent_Identity=25.78125, Blast_Score=145, Evalue=5e-36,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42137; Mature: 42137

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIICNQNELNNAIQLVSKAVASRPTHPILANILLTADQGTNKISLTGFDLNLGIQTSFD
CEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEEEEEEECEEECCC
GTVKNSGAITIPSKLLSEIVNKLPNESPVSLEVDENLDNILIKSDRGSFNLKGIPSDDYP
CCCCCCCEEEEHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCEEECCCCCCCCC
NLPFVESGTSLNIDPSSFLKALKATIFATSNDDSKQLLTGVNFTFKQNYLESASTDGHRL
CCCEEECCCEECCCHHHHHHHHHHEEEEECCCCHHHHHHCCCEEEHHHHHHHCCCCCCEE
AVALIGNEEHIENKDKSPSIEDDLSVTIPTRSLREIEKLVSLRSSENSIKLFYDKGQVVF
EEEEECCHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCEEEE
ISSNQIITTRTLEGAYPNYSQLIPDTFSKIFNFNTKKLIDALERIAVLADQQSSVVKIKL
EECCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
DNTDLASISADAQDIGNANESIPVSYSGEDFDIAFNVRYLLEGLKVIASKNVLLKCNLAT
CCCCCEEECCCHHHHCCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHHCCCEEEEEECCC
TPAVFVPEDNLNSFTYLVMPVQVRS
CCEEEEECCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MEIICNQNELNNAIQLVSKAVASRPTHPILANILLTADQGTNKISLTGFDLNLGIQTSFD
CEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEEEEEEECEEECCC
GTVKNSGAITIPSKLLSEIVNKLPNESPVSLEVDENLDNILIKSDRGSFNLKGIPSDDYP
CCCCCCCEEEEHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCEEECCCCCCCCC
NLPFVESGTSLNIDPSSFLKALKATIFATSNDDSKQLLTGVNFTFKQNYLESASTDGHRL
CCCEEECCCEECCCHHHHHHHHHHEEEEECCCCHHHHHHCCCEEEHHHHHHHCCCCCCEE
AVALIGNEEHIENKDKSPSIEDDLSVTIPTRSLREIEKLVSLRSSENSIKLFYDKGQVVF
EEEEECCHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCEEEE
ISSNQIITTRTLEGAYPNYSQLIPDTFSKIFNFNTKKLIDALERIAVLADQQSSVVKIKL
EECCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
DNTDLASISADAQDIGNANESIPVSYSGEDFDIAFNVRYLLEGLKVIASKNVLLKCNLAT
CCCCCEEECCCHHHHCCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHHCCCEEEEEECCC
TPAVFVPEDNLNSFTYLVMPVQVRS
CCEEEEECCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8654968 [H]