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The map label for this gene is bioB

Identifier: 78357697

GI number: 78357697

Start: 2669096

End: 2670070

Strand: Direct

Name: bioB

Synonym: Dde_2655

Alternate gene names: 78357697

Gene position: 2669096-2670070 (Clockwise)

Preceding gene: 78357695

Following gene: 78357698

Centisome position: 71.55

GC content: 63.08

Gene sequence:

>975_bases
ATGACCATAAACCCTGACGACCTGTGCCGCCGCATTATGGCGGACCCTGAAGCCTGTGCCACGGAAGATGAGGCACTGCG
GCTTCTGGAACTGCCCGCCGAGCAGACTCTGCCGCTTATCGCCTGTGCCCAGCGCATACGCACGGCACATGCCGGACCGG
CGTTCACCTGTGCCATTGTCAACGCCCGTTCCGGACGCTGCCCCGAAAACTGTTCATTCTGCGCACAGTCGGCCCATTAT
GCCACAGGTGCGCCCGAACACCCCATGCTGAACACCGCCGCACTGGTGGAGCACGCCCTGAAGCTGGCCGACGCCGGTGC
GGACAGATTCGGCATTGTGACCAGCGGCACACGCCCCGCCCCCCGCGAACTGGAAACAGTATGCGAAGCCGTGGTCCGCA
TACGGGCGCGCACGCACCTTTCGCCCTGCGCATCACTGGGCCAGCTCAGCCCGCAGGCAGCTGCAATGCTGCGGCAGGCG
GGACTGGAGCGCTACCACCATAATCTGGAAACCGCACGCAGTTTCTTTGCATCGGTATGCACAACGCATGACTACGACGA
AGACATATATACCGTCAAACTGGCGCGCGAGGCCGGACTGGCTGTGTGCTGCGGAGGCATACTGGGGCTGGGCGAAAGCC
GCGCGCAACGGGCGGAGCTTTCCGGTCAGATAAAACGGTGCAGGGCAGATTCGGTGCCGGTCAACCTGCTGACCCCTGTG
GCAGGCACGCCGCTGGAGCGCATGGATCCGCTGCCGCCGTTTGAAGCGCTGCGCACCATAGCCGTATTCAGATTCATGCA
TCCGCAGGCCGACATACTGGTGGCCGGAGGCCGCGAACATGTGCTGGGCGAATACCGTTCGTGGACATTCATCAGCGGCG
CCAACGGGCTGATGGTGGGCAATTATCTGACAACCGCGGGCCGCAGCACCGCTGACGACTACACCATGCTGCGCCATATG
GGAGTACTCCGATGA

Upstream 100 bases:

>100_bases
AAAAAGTTGACAATAAGCCGTCACGCAGGCTAGGAGTCGGCCGTTGACCGCGCCGGGCGGTCTTTCCTGACATGCTGCCA
CGCTGCAAGGATACACGCCC

Downstream 100 bases:

>100_bases
ACGCACTTCAACCCGACACGGAACGGCTGCGCGCACTGGACACGGCCCATGTATGGCACCCCTTCACCCAGATGGCGACG
TGGCACGAAGACGCGCCGCT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTINPDDLCRRIMADPEACATEDEALRLLELPAEQTLPLIACAQRIRTAHAGPAFTCAIVNARSGRCPENCSFCAQSAHY
ATGAPEHPMLNTAALVEHALKLADAGADRFGIVTSGTRPAPRELETVCEAVVRIRARTHLSPCASLGQLSPQAAAMLRQA
GLERYHHNLETARSFFASVCTTHDYDEDIYTVKLAREAGLAVCCGGILGLGESRAQRAELSGQIKRCRADSVPVNLLTPV
AGTPLERMDPLPPFEALRTIAVFRFMHPQADILVAGGREHVLGEYRSWTFISGANGLMVGNYLTTAGRSTADDYTMLRHM
GVLR

Sequences:

>Translated_324_residues
MTINPDDLCRRIMADPEACATEDEALRLLELPAEQTLPLIACAQRIRTAHAGPAFTCAIVNARSGRCPENCSFCAQSAHY
ATGAPEHPMLNTAALVEHALKLADAGADRFGIVTSGTRPAPRELETVCEAVVRIRARTHLSPCASLGQLSPQAAAMLRQA
GLERYHHNLETARSFFASVCTTHDYDEDIYTVKLAREAGLAVCCGGILGLGESRAQRAELSGQIKRCRADSVPVNLLTPV
AGTPLERMDPLPPFEALRTIAVFRFMHPQADILVAGGREHVLGEYRSWTFISGANGLMVGNYLTTAGRSTADDYTMLRHM
GVLR
>Mature_323_residues
TINPDDLCRRIMADPEACATEDEALRLLELPAEQTLPLIACAQRIRTAHAGPAFTCAIVNARSGRCPENCSFCAQSAHYA
TGAPEHPMLNTAALVEHALKLADAGADRFGIVTSGTRPAPRELETVCEAVVRIRARTHLSPCASLGQLSPQAAAMLRQAG
LERYHHNLETARSFFASVCTTHDYDEDIYTVKLAREAGLAVCCGGILGLGESRAQRAELSGQIKRCRADSVPVNLLTPVA
GTPLERMDPLPPFEALRTIAVFRFMHPQADILVAGGREHVLGEYRSWTFISGANGLMVGNYLTTAGRSTADDYTMLRHMG
VLR

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=301, Percent_Identity=33.8870431893688, Blast_Score=178, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321725, Length=278, Percent_Identity=32.3741007194245, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_DESDG (Q30XZ5)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389146.1
- ProteinModelPortal:   Q30XZ5
- SMR:   Q30XZ5
- STRING:   Q30XZ5
- GeneID:   3757678
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2655
- NMPDR:   fig|207559.3.peg.2910
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   LEICSGG
- ProtClustDB:   CLSK705864
- BioCyc:   DDES207559:DDE_2655-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35067; Mature: 34935

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINPDDLCRRIMADPEACATEDEALRLLELPAEQTLPLIACAQRIRTAHAGPAFTCAIV
CCCCHHHHHHHHHCCCHHHCCHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCEEEEEE
NARSGRCPENCSFCAQSAHYATGAPEHPMLNTAALVEHALKLADAGADRFGIVTSGTRPA
ECCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
PRELETVCEAVVRIRARTHLSPCASLGQLSPQAAAMLRQAGLERYHHNLETARSFFASVC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTHDYDEDIYTVKLAREAGLAVCCGGILGLGESRAQRAELSGQIKRCRADSVPVNLLTPV
HCCCCCCCEEEEEEHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCC
AGTPLERMDPLPPFEALRTIAVFRFMHPQADILVAGGREHVLGEYRSWTFISGANGLMVG
CCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCEEEEECCCCEEEC
NYLTTAGRSTADDYTMLRHMGVLR
CCHHHCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TINPDDLCRRIMADPEACATEDEALRLLELPAEQTLPLIACAQRIRTAHAGPAFTCAIV
CCCHHHHHHHHHCCCHHHCCHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCEEEEEE
NARSGRCPENCSFCAQSAHYATGAPEHPMLNTAALVEHALKLADAGADRFGIVTSGTRPA
ECCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
PRELETVCEAVVRIRARTHLSPCASLGQLSPQAAAMLRQAGLERYHHNLETARSFFASVC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTHDYDEDIYTVKLAREAGLAVCCGGILGLGESRAQRAELSGQIKRCRADSVPVNLLTPV
HCCCCCCCEEEEEEHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCC
AGTPLERMDPLPPFEALRTIAVFRFMHPQADILVAGGREHVLGEYRSWTFISGANGLMVG
CCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCEEEEECCCCEEEC
NYLTTAGRSTADDYTMLRHMGVLR
CCHHHCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA