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The map label for this gene is hemC

Identifier: 78357663

GI number: 78357663

Start: 2636103

End: 2637053

Strand: Direct

Name: hemC

Synonym: Dde_2621

Alternate gene names: 78357663

Gene position: 2636103-2637053 (Clockwise)

Preceding gene: 78357662

Following gene: 78357668

Centisome position: 70.67

GC content: 60.78

Gene sequence:

>951_bases
ATGAAAAAAATCGTCATCGCTACCCGCGGCAGCAAGCTCGCTCTCTGGCAGGCAGAACATATCAAAGAACGCATCATGCA
GCAGTACCCCGACAGTTTTGAAGTCGAACTGCTGGTTCTGAAAACCAAAGGCGACATCATTCTTGACGTGCCCCTGGCCA
AAGTGGGCGGCAAGGGTCTTTTTGTCAAAGAAATCGAAGAAGCGCTGCTGGACGGCCGCGCAGACATCGCCGTGCACAGC
ATGAAAGATGTTCCCATGGAACTGCCTCAGGGACTGACTCTGGGAGCCATTCCCGAGAGGGAAGTCCCCACGGACTCGCT
GCTTTCGGCCAGATACGACGGGCTGGACGCCCTGCCGCAGGGTGCCGTGGTGGGCACAAGCAGTCTGCGCCGCCAGTCAC
AGATTCTCGCCATGCGTCCCGACCTTGAAGTCAAATGGCTGCGCGGCAACGTGCAGACCCGCATACGCAAACTGATGGAC
GGAGAATACGACGCCATCATCATGGCCACATCAGGGCTCAAGCGTCTGGGCGAAACGGCTCCCTTTCATTCCGAACTGGG
ACCGCCCCTGTTCATGCCCGCCGTGGGACAGGGAGCACTGGGTCTTGAATACCGCAGTGACAGGCAGGACCTTGCCGAGA
TGCTGGCATTTCTTGACCACCGCCCCTCGCGCATATGCGTCGAGGCGGAACGCGGCTTTCTTTTCGGGCTGCAGGGCGGC
TGTCAGGTGCCCATAGCCGGATACGCCGTCATGACCGGCGACAACAGCTTTGAGCTGACGGGACTTGTGGCCGAACTGGA
CGGCACCGCCGTCATCCGGCGCACTGCCGCGGGCACCGAAGCAGACGCCCGCGATACCGGTCTGACGCTTGCAGCACAGG
TAAAGGCCGCCGGTGCTGACGCCATTCTGCAGCGCGTCTATGAAAACGGCCACGCCTCGCCGCAGGCCTGA

Upstream 100 bases:

>100_bases
GCCGGCATCATCCGGCGGCTGTTCCGGCTGTTCCGGCGGCAACTGCTCTTCCTGCAAATAACGCCGCCCCGCCAATTCTC
TAACTCTCACAGGCGCGCAC

Downstream 100 bases:

>100_bases
GTCTGACAAACGCCATACAAAACGGGCGGCGCAGATAATATCTGCGCCGCCCGTTTCTTTCAGAAAGAAACTCCGGAAAA
TCAGGGCACCATTTCCACCT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKKIVIATRGSKLALWQAEHIKERIMQQYPDSFEVELLVLKTKGDIILDVPLAKVGGKGLFVKEIEEALLDGRADIAVHS
MKDVPMELPQGLTLGAIPEREVPTDSLLSARYDGLDALPQGAVVGTSSLRRQSQILAMRPDLEVKWLRGNVQTRIRKLMD
GEYDAIIMATSGLKRLGETAPFHSELGPPLFMPAVGQGALGLEYRSDRQDLAEMLAFLDHRPSRICVEAERGFLFGLQGG
CQVPIAGYAVMTGDNSFELTGLVAELDGTAVIRRTAAGTEADARDTGLTLAAQVKAAGADAILQRVYENGHASPQA

Sequences:

>Translated_316_residues
MKKIVIATRGSKLALWQAEHIKERIMQQYPDSFEVELLVLKTKGDIILDVPLAKVGGKGLFVKEIEEALLDGRADIAVHS
MKDVPMELPQGLTLGAIPEREVPTDSLLSARYDGLDALPQGAVVGTSSLRRQSQILAMRPDLEVKWLRGNVQTRIRKLMD
GEYDAIIMATSGLKRLGETAPFHSELGPPLFMPAVGQGALGLEYRSDRQDLAEMLAFLDHRPSRICVEAERGFLFGLQGG
CQVPIAGYAVMTGDNSFELTGLVAELDGTAVIRRTAAGTEADARDTGLTLAAQVKAAGADAILQRVYENGHASPQA
>Mature_316_residues
MKKIVIATRGSKLALWQAEHIKERIMQQYPDSFEVELLVLKTKGDIILDVPLAKVGGKGLFVKEIEEALLDGRADIAVHS
MKDVPMELPQGLTLGAIPEREVPTDSLLSARYDGLDALPQGAVVGTSSLRRQSQILAMRPDLEVKWLRGNVQTRIRKLMD
GEYDAIIMATSGLKRLGETAPFHSELGPPLFMPAVGQGALGLEYRSDRQDLAEMLAFLDHRPSRICVEAERGFLFGLQGG
CQVPIAGYAVMTGDNSFELTGLVAELDGTAVIRRTAAGTEADARDTGLTLAAQVKAAGADAILQRVYENGHASPQA

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=289, Percent_Identity=43.2525951557093, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI66933009, Length=285, Percent_Identity=43.5087719298246, Blast_Score=206, Evalue=3e-53,
Organism=Escherichia coli, GI48994974, Length=307, Percent_Identity=54.3973941368078, Blast_Score=305, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6319996, Length=319, Percent_Identity=36.9905956112853, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI20130425, Length=286, Percent_Identity=40.9090909090909, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_DESDG (Q30Y29)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389112.1
- HSSP:   P06983
- ProteinModelPortal:   Q30Y29
- SMR:   Q30Y29
- STRING:   Q30Y29
- GeneID:   3757643
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2621
- NMPDR:   fig|207559.3.peg.2808
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   DDES207559:DDE_2621-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34171; Mature: 34171

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVIATRGSKLALWQAEHIKERIMQQYPDSFEVELLVLKTKGDIILDVPLAKVGGKGL
CCEEEEEECCCEEEEEEHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEECHHHHCCCCE
FVKEIEEALLDGRADIAVHSMKDVPMELPQGLTLGAIPEREVPTDSLLSARYDGLDALPQ
EHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCEECCCCCCCCCHHHHHHHHCCCCCCCCC
GAVVGTSSLRRQSQILAMRPDLEVKWLRGNVQTRIRKLMDGEYDAIIMATSGLKRLGETA
CCEECCHHHHHHHHEEEECCCCEEEEECCCHHHHHHHHHCCCCCEEEEECCCHHHHCCCC
PFHSELGPPLFMPAVGQGALGLEYRSDRQDLAEMLAFLDHRPSRICVEAERGFLFGLQGG
CCHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCC
CQVPIAGYAVMTGDNSFELTGLVAELDGTAVIRRTAAGTEADARDTGLTLAAQVKAAGAD
CCCCCCCEEEEECCCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCCEEEEEEEHHCCHH
AILQRVYENGHASPQA
HHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKIVIATRGSKLALWQAEHIKERIMQQYPDSFEVELLVLKTKGDIILDVPLAKVGGKGL
CCEEEEEECCCEEEEEEHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEECHHHHCCCCE
FVKEIEEALLDGRADIAVHSMKDVPMELPQGLTLGAIPEREVPTDSLLSARYDGLDALPQ
EHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCEECCCCCCCCCHHHHHHHHCCCCCCCCC
GAVVGTSSLRRQSQILAMRPDLEVKWLRGNVQTRIRKLMDGEYDAIIMATSGLKRLGETA
CCEECCHHHHHHHHEEEECCCCEEEEECCCHHHHHHHHHCCCCCEEEEECCCHHHHCCCC
PFHSELGPPLFMPAVGQGALGLEYRSDRQDLAEMLAFLDHRPSRICVEAERGFLFGLQGG
CCHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCC
CQVPIAGYAVMTGDNSFELTGLVAELDGTAVIRRTAAGTEADARDTGLTLAAQVKAAGAD
CCCCCCCEEEEECCCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCCEEEEEEEHHCCHH
AILQRVYENGHASPQA
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA