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The map label for this gene is appA [H]

Identifier: 78356608

GI number: 78356608

Start: 1587525

End: 1588670

Strand: Direct

Name: appA [H]

Synonym: Dde_1565

Alternate gene names: 78356608

Gene position: 1587525-1588670 (Clockwise)

Preceding gene: 78356606

Following gene: 78356609

Centisome position: 42.56

GC content: 53.32

Gene sequence:

>1146_bases
ATGAATCGTGTTTATGTTGTTTTTGTCAGCATGCTGTTTTTGCTGACTGTCACGGCATGTGACAACAGTGCCGGTGACAA
GCCTTCCGGCGGAACCGCCGGAAACGCCACTGTGGTTTCTGCCGTCACAGGTTCCCCCTCCGGAACGCAGAATGACGGCG
AACCAGCCTACGGTGGCCGTATAATCATAGGGTCCATAGGCGAACCCACCAACCTTATCAGCGCGCTTGCGTCAGACAGT
GTCTCGCGCGAGATAACCAGCTGGCTTTACATAGGCCTGCTGCAATACGACAAAAATCTGGAGATTGTTCCGTGGGCTGC
CGAATCCTATGACGTGGAAGAAGGCGGAACGCTGTTGCGTTTTCGACTCCGTAAAGGAATAAAATGGCAGGACGGTACAG
AACTGACAGCAGACGACGTCGAATTTACCTACAGGCTGATGATTGACCCCAAAACCCCCACGGCGTACGCCAGTGATTTT
CTGGCGGTAAAGGAATTCCGTAAAGTAGACCGGTACACCGTAGAGGTGCGCTACGAAAAACCTTTTGCGCGTTCCCTCAT
CACATGGATGAGTGACATACTGCCCAGGCACCGGCTGGAGGGGCAGGATCTGCTGACGACGCCGTTATTGCGAAAGCCCG
TGGGGGCTGGGCCCTACAGGCTGAAAGAATGGGTACCCGGAAGCAGGGTGGTGCTGGAAGCCAGCGACACCTACTTTGAA
GGGCGCCCGTATATTGATGAGGTGGTTTACCGCAACATTCCTGATTTGGCTACCATGTTCCTTGAATTAAAAGCGGGCAG
GCTCGATATGATGTCGCTTACCCCGCAGCAGTATAAATTTCAGACAACCGGCGGTGACTGGGACACACAGTGGCATAAAT
TCCGCTACCTGTCTTTCGGCTATGCTTTTCTGGGGTATAATATGGAGCGCAGCCCCTTTGACGAAGCACCTGTGCGCCGT
GCGCTTTCCTGCGCCATAGACAGGAATGCGCTTATCGCGGGTGTGCTGTTGGGGGAAGGTCGTGCCACCGTAGGGCCTTA
CAAACCGGGAACATGGGTATATAATGACAAGCTTGCGGATTACCCCTATCAGGGTGTTGAAAAATTCCCATCCTTGCTCC
TGGACATAAAATTGACTTCGTCATGA

Upstream 100 bases:

>100_bases
ACGGAACTTTGGTAGATGTTTATCATAGGTCGTGCGACCTGTGCCCCATCGACCGAGAATACCGTATCCGCCGGAATTGG
CAATAGCCTGCGAGGTTTTC

Downstream 100 bases:

>100_bases
ACCGTCCTGTAGTTTCTTTGGAAGGAAACATGCTATTGATTAAATTGAGCTGACCCAGGAGATAAAAATGTCCGACCTTA
TTTGCTATTGTTTTGGTTAC

Product: oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MNRVYVVFVSMLFLLTVTACDNSAGDKPSGGTAGNATVVSAVTGSPSGTQNDGEPAYGGRIIIGSIGEPTNLISALASDS
VSREITSWLYIGLLQYDKNLEIVPWAAESYDVEEGGTLLRFRLRKGIKWQDGTELTADDVEFTYRLMIDPKTPTAYASDF
LAVKEFRKVDRYTVEVRYEKPFARSLITWMSDILPRHRLEGQDLLTTPLLRKPVGAGPYRLKEWVPGSRVVLEASDTYFE
GRPYIDEVVYRNIPDLATMFLELKAGRLDMMSLTPQQYKFQTTGGDWDTQWHKFRYLSFGYAFLGYNMERSPFDEAPVRR
ALSCAIDRNALIAGVLLGEGRATVGPYKPGTWVYNDKLADYPYQGVEKFPSLLLDIKLTSS

Sequences:

>Translated_381_residues
MNRVYVVFVSMLFLLTVTACDNSAGDKPSGGTAGNATVVSAVTGSPSGTQNDGEPAYGGRIIIGSIGEPTNLISALASDS
VSREITSWLYIGLLQYDKNLEIVPWAAESYDVEEGGTLLRFRLRKGIKWQDGTELTADDVEFTYRLMIDPKTPTAYASDF
LAVKEFRKVDRYTVEVRYEKPFARSLITWMSDILPRHRLEGQDLLTTPLLRKPVGAGPYRLKEWVPGSRVVLEASDTYFE
GRPYIDEVVYRNIPDLATMFLELKAGRLDMMSLTPQQYKFQTTGGDWDTQWHKFRYLSFGYAFLGYNMERSPFDEAPVRR
ALSCAIDRNALIAGVLLGEGRATVGPYKPGTWVYNDKLADYPYQGVEKFPSLLLDIKLTSS
>Mature_381_residues
MNRVYVVFVSMLFLLTVTACDNSAGDKPSGGTAGNATVVSAVTGSPSGTQNDGEPAYGGRIIIGSIGEPTNLISALASDS
VSREITSWLYIGLLQYDKNLEIVPWAAESYDVEEGGTLLRFRLRKGIKWQDGTELTADDVEFTYRLMIDPKTPTAYASDF
LAVKEFRKVDRYTVEVRYEKPFARSLITWMSDILPRHRLEGQDLLTTPLLRKPVGAGPYRLKEWVPGSRVVLEASDTYFE
GRPYIDEVVYRNIPDLATMFLELKAGRLDMMSLTPQQYKFQTTGGDWDTQWHKFRYLSFGYAFLGYNMERSPFDEAPVRR
ALSCAIDRNALIAGVLLGEGRATVGPYKPGTWVYNDKLADYPYQGVEKFPSLLLDIKLTSS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=342, Percent_Identity=28.9473684210526, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787762, Length=316, Percent_Identity=25.6329113924051, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787052, Length=322, Percent_Identity=25.4658385093168, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787551, Length=342, Percent_Identity=26.6081871345029, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787495, Length=285, Percent_Identity=28.7719298245614, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789397, Length=298, Percent_Identity=24.496644295302, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789887, Length=264, Percent_Identity=23.4848484848485, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081878, Length=303, Percent_Identity=24.4224422442244, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87082063, Length=288, Percent_Identity=23.9583333333333, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 42683; Mature: 42683

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVYVVFVSMLFLLTVTACDNSAGDKPSGGTAGNATVVSAVTGSPSGTQNDGEPAYGGR
CCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCE
IIIGSIGEPTNLISALASDSVSREITSWLYIGLLQYDKNLEIVPWAAESYDVEEGGTLLR
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCEEE
FRLRKGIKWQDGTELTADDVEFTYRLMIDPKTPTAYASDFLAVKEFRKVDRYTVEVRYEK
EHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
PFARSLITWMSDILPRHRLEGQDLLTTPLLRKPVGAGPYRLKEWVPGSRVVLEASDTYFE
HHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCCCCHHHHHCCCCCEEEEEECCCEEC
GRPYIDEVVYRNIPDLATMFLELKAGRLDMMSLTPQQYKFQTTGGDWDTQWHKFRYLSFG
CCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCHHEEEEHEEE
YAFLGYNMERSPFDEAPVRRALSCAIDRNALIAGVLLGEGRATVGPYKPGTWVYNDKLAD
EHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCEEECCCCCC
YPYQGVEKFPSLLLDIKLTSS
CCHHHHHHHHHEEEEEEEECC
>Mature Secondary Structure
MNRVYVVFVSMLFLLTVTACDNSAGDKPSGGTAGNATVVSAVTGSPSGTQNDGEPAYGGR
CCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCE
IIIGSIGEPTNLISALASDSVSREITSWLYIGLLQYDKNLEIVPWAAESYDVEEGGTLLR
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCEEE
FRLRKGIKWQDGTELTADDVEFTYRLMIDPKTPTAYASDFLAVKEFRKVDRYTVEVRYEK
EHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
PFARSLITWMSDILPRHRLEGQDLLTTPLLRKPVGAGPYRLKEWVPGSRVVLEASDTYFE
HHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCCCCHHHHHCCCCCEEEEEECCCEEC
GRPYIDEVVYRNIPDLATMFLELKAGRLDMMSLTPQQYKFQTTGGDWDTQWHKFRYLSFG
CCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCHHEEEEHEEE
YAFLGYNMERSPFDEAPVRRALSCAIDRNALIAGVLLGEGRATVGPYKPGTWVYNDKLAD
EHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCEEECCCCCC
YPYQGVEKFPSLLLDIKLTSS
CCHHHHHHHHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]