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The map label for this gene is opuAB [H]

Identifier: 78355721

GI number: 78355721

Start: 691016

End: 691855

Strand: Direct

Name: opuAB [H]

Synonym: Dde_0674

Alternate gene names: 78355721

Gene position: 691016-691855 (Clockwise)

Preceding gene: 78355720

Following gene: 78355722

Centisome position: 18.52

GC content: 59.05

Gene sequence:

>840_bases
ATGATTGATTATCGCATTCCCGTTGGCGCTACGCTTGAAAGCGTCATTGATTTTCTGGTCGTGCACTGCTCCGGCGCAAC
CCGTGCGTTTTCCGCGGTTACCGATGCCGCTCTTTCCGTGCTGGAACACGGTCTTGCCTTCATACCGTGGTGGCTGTTCA
TTCCTGCCGTCGCCCTGCTGACCTGGCGCGGCACCGGCAGCAGAGGGCTGGGGCTTTTTGCCCTGCTGGGGCTGGGACTT
GTGTATAACATGGGGCTGTGGCCCGCCACGGTCAGCACCATTGCTCTGGTGCTTGTGGCCACGCTGCTTGCGGTGCTTAT
CGGTCTGCCGCTGGGTGTTTTTGCCGCTATCAATAAAGTGGCATACCGTGCTATCATGCCGGTACTCGATGTAATGCAGA
CCATGCCCGCCTTTGTTTATCTTATTCCCGCCATTCCGTTTTTCGGGCTGGGCAAGGTTGCGGCCATTTTTTCCACTGTG
GTGTTCGCCATGCCGCCGGTCATCAGGCTTACCTGCCTTGGTATCCGGCAGGTTCCGGTAGAGCTGGTGGAGTGCGCCGA
AGCTTTCGGCACCAACCGCTGGCAGCGGCTGCGCAAGCTCGAACTGCCTCTGGCGGCGCCCACCATCATGGCGGGTGTCA
ACCAGACTGTCATGCTGGCGCTGTCCATGGTGGTCATTGCAGCCATGATCGGTGCAAAGGGTCTTGGCGGCGAAGTATGG
AAAGCCATTCAGCGCCTTGAGATGGGCCGCGGTTTTGAAGCCGGTATCGGCATTGTCATCGTTGCCATCTGCCTTGACCG
GCTGCTGCAGAAAATCGGCTCGCGCTCAGCCAGACGCTAG

Upstream 100 bases:

>100_bases
CCCATGCACTGCCTGTTGTGGACGAGAAAAACCGCATGAAAGGTGTCATTGTGCGCGGCCTTCTGCTGGGGGCGCTGGCT
GAAAGAGGAGGTGACCAGTA

Downstream 100 bases:

>100_bases
CCGCAGGTGCGGGCCGGATTCCGCAGTGGTGTCCGGCCTTTCATCAGGATGCCGGCAGGGTATTCTGTTTCCGTAAGAAT
GTAATTCCGGAGGAACACAT

Product: glycine/betaine/L-proline ABC transporter permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MIDYRIPVGATLESVIDFLVVHCSGATRAFSAVTDAALSVLEHGLAFIPWWLFIPAVALLTWRGTGSRGLGLFALLGLGL
VYNMGLWPATVSTIALVLVATLLAVLIGLPLGVFAAINKVAYRAIMPVLDVMQTMPAFVYLIPAIPFFGLGKVAAIFSTV
VFAMPPVIRLTCLGIRQVPVELVECAEAFGTNRWQRLRKLELPLAAPTIMAGVNQTVMLALSMVVIAAMIGAKGLGGEVW
KAIQRLEMGRGFEAGIGIVIVAICLDRLLQKIGSRSARR

Sequences:

>Translated_279_residues
MIDYRIPVGATLESVIDFLVVHCSGATRAFSAVTDAALSVLEHGLAFIPWWLFIPAVALLTWRGTGSRGLGLFALLGLGL
VYNMGLWPATVSTIALVLVATLLAVLIGLPLGVFAAINKVAYRAIMPVLDVMQTMPAFVYLIPAIPFFGLGKVAAIFSTV
VFAMPPVIRLTCLGIRQVPVELVECAEAFGTNRWQRLRKLELPLAAPTIMAGVNQTVMLALSMVVIAAMIGAKGLGGEVW
KAIQRLEMGRGFEAGIGIVIVAICLDRLLQKIGSRSARR
>Mature_279_residues
MIDYRIPVGATLESVIDFLVVHCSGATRAFSAVTDAALSVLEHGLAFIPWWLFIPAVALLTWRGTGSRGLGLFALLGLGL
VYNMGLWPATVSTIALVLVATLLAVLIGLPLGVFAAINKVAYRAIMPVLDVMQTMPAFVYLIPAIPFFGLGKVAAIFSTV
VFAMPPVIRLTCLGIRQVPVELVECAEAFGTNRWQRLRKLELPLAAPTIMAGVNQTVMLALSMVVIAAMIGAKGLGGEVW
KAIQRLEMGRGFEAGIGIVIVAICLDRLLQKIGSRSARR

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=274, Percent_Identity=45.985401459854, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1788449, Length=181, Percent_Identity=30.3867403314917, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788451, Length=198, Percent_Identity=28.2828282828283, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29840; Mature: 29840

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure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HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]