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The map label for this gene is nifA [H]

Identifier: 78355691

GI number: 78355691

Start: 662940

End: 664991

Strand: Direct

Name: nifA [H]

Synonym: Dde_0644

Alternate gene names: 78355691

Gene position: 662940-664991 (Clockwise)

Preceding gene: 78355690

Following gene: 78355692

Centisome position: 17.77

GC content: 60.96

Gene sequence:

>2052_bases
ATGAATCAGCAAGGGGCAGAAACGGAGCAGGATCTGCGGGCGCGGGTGCCGGACGAGCGCAGAGCCACGCTGGCCGCGCT
GGAGCTGGCAGCCACCCTGAGCCATTTTGACCAGCGGCTTGACCGTATGGAAGGAGTGGACAGCATTCTGCGAGAGACCG
CGGCCAAGGCTCAGGGACTGGTGGAAATGCAGTCATGGGCTTTTTTTCTGGTCGATCCGGAAACCGGAGATTTTACCTGT
GCCCTTACTGACGGGCCGGAAGACCGTCTGCGTTTTGAACGTGAAGTGGACGCACTGATAGAAGACAGAACCTTTGCGTG
GGTTCTGGGCCGCAACAGGGCCGTGCTTGTGCCTTCGGGCATCGGCGCTGCCGGCAGGCTGCTGCTCCATCCGCTGGCGA
CATCTTCGGGGCTGTGGGGCATGTTTGTGGCTTTTACCGGTGAAGGGGCAGAGTCCACAGACCTCGGGTTTTATCTGCTT
ACGGTGGTGTTTTTTTCCTGCGCGTCCATGCTTGAAAGCTATTCTCTGTACCGCCAGCTGCAGCAGGTGAACGAGGGACT
GGAACAGCAGGTGGCCGACCGCACCCGCGAACTGGTGTCCATTAACTCCAGTCTGGAACGCGAGGTGGAGGAACGCAGTC
TGGCAGAGCAGCAGCTGCGGCGGACTCTGAACGAAAAAGAGTCATACCGGCAGCATCTTGAGGCCGTTTTTTCCAGCATA
CAGGATGCCATCATCACCGTAGACCTGAATGGTGTGGTACTGAACATCAATGCCGCTGCTGAAAAAATGCTGGGCATTCC
GGCTGCACAGGCTGTGAGAATGCATCATACCGCCCTGCAGCTGCCCTGTGCCAGAGCATGTCAGGATGTGCTGGGAGCCA
CGCTGGGTTCCGGTACGCCCGTGCGCGAATTCCGCGCCGTGAGTGAAGACGGCGCTCAGGTGATGATTCTGGGCGGCACG
CCGCTTATCTCGCTGGACGGGACGTTTTCCGGCGCCGTTCTTTCCTGCCGCGATATCACGCGGCTTGTGTCGCTGGAGCA
GCAGCTCCGTCAGCGGCATTCATTCAGCAATATCATCGGGCGCAGCAAGGTTATGCAATCGCTGTTCGGCCTGCTGGAAA
ATCTTGCGGCATACGACACCACAGTGCTGGTCACCGGTGAATCCGGTACCGGCAAGGAACTGGTGGCAGAGGCTCTGCAT
TACAACGGGGCGCGCTCGGGCGGACCGCTGGTCAAGGTGAACTGCACTGCCTTGTCTGAAAGCCTGCTGGAAAGCGAATT
GTTCGGCCATGTGCGCGGGGCCTTTACCGGAGCCGTGCGCGACAGGGCCGGACGGTTTGAGACCGCCGAAGGCGGTACTA
TTTTTCTTGATGAGATCGGCGACATCTCCATGCGTATTCAGGTACTGCTGCTGCGCTTTCTCGAGTCAAAAGAATTTGAA
CGGGTGGGTGACACCACTCCGCGGCGGGCTGATGTGCGCATAGTGGCTGCCACCAATGCCGACCTGCACCGGCGCATCAG
TGAAGGTACTTTCCGCGCGGACCTGTTTTACCGGCTCAATGTCACCAGTGTACATCTGCCGCCGCTCAGGGAGAGGCGCG
AAGACATCCCGCTGCTGGTCCAGCATTTTGTGGAGCAATGCAACACCGAACTGGGAACCCGTGTGGCCGGAGTGGATGAA
AAAGCCATGGCTGCGCTGGTCCGTGCTCCGTGGCCGGGCAATGTGCGCGAACTGAAGCATACGCTGGAACATGCCTGCGT
GCTGTGCCGCGACGGCTATGTGGAAGCCGGTCACCTGCCGGCGGAGCTTGCGGCGGTTGAGTCTCCCCCTGTCCGGTTTG
ATGCGGCGGTGTCCGGGCCGGTTTCTGCCGCAGCGGGCGGCGCAGTTCCGCAGCCCGTGGCGGGCGGATTCGGACGGCGG
CAGCTGGACGCCCGCAGCATCACGGCGGCCATCGAACAGGCCGGAGGCAACAAGGCCAGAGCGGCGCGCCTGCTGGGGGT
AGGGCGGGCGACTCTGTACCGCAAACTGCGGGAGCTGGACATGTCCATATAA

Upstream 100 bases:

>100_bases
CGCCTTCGGCTGTGCAGACAATGATGGCGCAGGCGGGCAGGCAGTTTGAGGCCATTTCCGGTGTGTTCTTTGACACCGGT
CAGGAAGCGCGGGACAAGGC

Downstream 100 bases:

>100_bases
CATGTCTCAAGTGTCTCAAGTGAGACATCTTTGTGTCTCAAGTGTCTTGTTTGAGACAGCGATTAGGTGTCTCACTGACA
GGATTATTCTTAGTGGAAAT

Product: Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 683; Mature: 683

Protein sequence:

>683_residues
MNQQGAETEQDLRARVPDERRATLAALELAATLSHFDQRLDRMEGVDSILRETAAKAQGLVEMQSWAFFLVDPETGDFTC
ALTDGPEDRLRFEREVDALIEDRTFAWVLGRNRAVLVPSGIGAAGRLLLHPLATSSGLWGMFVAFTGEGAESTDLGFYLL
TVVFFSCASMLESYSLYRQLQQVNEGLEQQVADRTRELVSINSSLEREVEERSLAEQQLRRTLNEKESYRQHLEAVFSSI
QDAIITVDLNGVVLNINAAAEKMLGIPAAQAVRMHHTALQLPCARACQDVLGATLGSGTPVREFRAVSEDGAQVMILGGT
PLISLDGTFSGAVLSCRDITRLVSLEQQLRQRHSFSNIIGRSKVMQSLFGLLENLAAYDTTVLVTGESGTGKELVAEALH
YNGARSGGPLVKVNCTALSESLLESELFGHVRGAFTGAVRDRAGRFETAEGGTIFLDEIGDISMRIQVLLLRFLESKEFE
RVGDTTPRRADVRIVAATNADLHRRISEGTFRADLFYRLNVTSVHLPPLRERREDIPLLVQHFVEQCNTELGTRVAGVDE
KAMAALVRAPWPGNVRELKHTLEHACVLCRDGYVEAGHLPAELAAVESPPVRFDAAVSGPVSAAAGGAVPQPVAGGFGRR
QLDARSITAAIEQAGGNKARAARLLGVGRATLYRKLRELDMSI

Sequences:

>Translated_683_residues
MNQQGAETEQDLRARVPDERRATLAALELAATLSHFDQRLDRMEGVDSILRETAAKAQGLVEMQSWAFFLVDPETGDFTC
ALTDGPEDRLRFEREVDALIEDRTFAWVLGRNRAVLVPSGIGAAGRLLLHPLATSSGLWGMFVAFTGEGAESTDLGFYLL
TVVFFSCASMLESYSLYRQLQQVNEGLEQQVADRTRELVSINSSLEREVEERSLAEQQLRRTLNEKESYRQHLEAVFSSI
QDAIITVDLNGVVLNINAAAEKMLGIPAAQAVRMHHTALQLPCARACQDVLGATLGSGTPVREFRAVSEDGAQVMILGGT
PLISLDGTFSGAVLSCRDITRLVSLEQQLRQRHSFSNIIGRSKVMQSLFGLLENLAAYDTTVLVTGESGTGKELVAEALH
YNGARSGGPLVKVNCTALSESLLESELFGHVRGAFTGAVRDRAGRFETAEGGTIFLDEIGDISMRIQVLLLRFLESKEFE
RVGDTTPRRADVRIVAATNADLHRRISEGTFRADLFYRLNVTSVHLPPLRERREDIPLLVQHFVEQCNTELGTRVAGVDE
KAMAALVRAPWPGNVRELKHTLEHACVLCRDGYVEAGHLPAELAAVESPPVRFDAAVSGPVSAAAGGAVPQPVAGGFGRR
QLDARSITAAIEQAGGNKARAARLLGVGRATLYRKLRELDMSI
>Mature_683_residues
MNQQGAETEQDLRARVPDERRATLAALELAATLSHFDQRLDRMEGVDSILRETAAKAQGLVEMQSWAFFLVDPETGDFTC
ALTDGPEDRLRFEREVDALIEDRTFAWVLGRNRAVLVPSGIGAAGRLLLHPLATSSGLWGMFVAFTGEGAESTDLGFYLL
TVVFFSCASMLESYSLYRQLQQVNEGLEQQVADRTRELVSINSSLEREVEERSLAEQQLRRTLNEKESYRQHLEAVFSSI
QDAIITVDLNGVVLNINAAAEKMLGIPAAQAVRMHHTALQLPCARACQDVLGATLGSGTPVREFRAVSEDGAQVMILGGT
PLISLDGTFSGAVLSCRDITRLVSLEQQLRQRHSFSNIIGRSKVMQSLFGLLENLAAYDTTVLVTGESGTGKELVAEALH
YNGARSGGPLVKVNCTALSESLLESELFGHVRGAFTGAVRDRAGRFETAEGGTIFLDEIGDISMRIQVLLLRFLESKEFE
RVGDTTPRRADVRIVAATNADLHRRISEGTFRADLFYRLNVTSVHLPPLRERREDIPLLVQHFVEQCNTELGTRVAGVDE
KAMAALVRAPWPGNVRELKHTLEHACVLCRDGYVEAGHLPAELAAVESPPVRFDAAVSGPVSAAAGGAVPQPVAGGFGRR
QLDARSITAAIEQAGGNKARAARLLGVGRATLYRKLRELDMSI

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=340, Percent_Identity=44.4117647058823, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788550, Length=347, Percent_Identity=42.0749279538905, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1790437, Length=344, Percent_Identity=44.1860465116279, Blast_Score=270, Evalue=3e-73,
Organism=Escherichia coli, GI1789087, Length=338, Percent_Identity=41.1242603550296, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI87082117, Length=339, Percent_Identity=39.8230088495575, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1789233, Length=326, Percent_Identity=38.9570552147239, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87082152, Length=336, Percent_Identity=42.2619047619048, Blast_Score=232, Evalue=6e-62,
Organism=Escherichia coli, GI1788905, Length=325, Percent_Identity=41.2307692307692, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87081872, Length=330, Percent_Identity=39.3939393939394, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=345, Percent_Identity=37.6811594202899, Blast_Score=199, Evalue=6e-52,
Organism=Escherichia coli, GI1787583, Length=357, Percent_Identity=34.1736694677871, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI87081858, Length=459, Percent_Identity=27.8867102396514, Blast_Score=166, Evalue=6e-42,
Organism=Escherichia coli, GI1789828, Length=296, Percent_Identity=33.445945945946, Blast_Score=132, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 74500; Mature: 74500

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQGAETEQDLRARVPDERRATLAALELAATLSHFDQRLDRMEGVDSILRETAAKAQGL
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEMQSWAFFLVDPETGDFTCALTDGPEDRLRFEREVDALIEDRTFAWVLGRNRAVLVPSG
CEEECCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECC
IGAAGRLLLHPLATSSGLWGMFVAFTGEGAESTDLGFYLLTVVFFSCASMLESYSLYRQL
CCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QQVNEGLEQQVADRTRELVSINSSLEREVEERSLAEQQLRRTLNEKESYRQHLEAVFSSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDAIITVDLNGVVLNINAAAEKMLGIPAAQAVRMHHTALQLPCARACQDVLGATLGSGTP
HHEEEEEEECCEEEEECHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
VREFRAVSEDGAQVMILGGTPLISLDGTFSGAVLSCRDITRLVSLEQQLRQRHSFSNIIG
HHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSKVMQSLFGLLENLAAYDTTVLVTGESGTGKELVAEALHYNGARSGGPLVKVNCTALSE
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHHHH
SLLESELFGHVRGAFTGAVRDRAGRFETAEGGTIFLDEIGDISMRIQVLLLRFLESKEFE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
RVGDTTPRRADVRIVAATNADLHRRISEGTFRADLFYRLNVTSVHLPPLRERREDIPLLV
HHCCCCCCCCCEEEEEECCHHHHHHHHCCCEEEEEEEEEEEEEEECCCHHHHHCCCHHHH
QHFVEQCNTELGTRVAGVDEKAMAALVRAPWPGNVRELKHTLEHACVLCRDGYVEAGHLP
HHHHHHHCHHHCCEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCC
AELAAVESPPVRFDAAVSGPVSAAAGGAVPQPVAGGFGRRQLDARSITAAIEQAGGNKAR
HHHHHCCCCCCEEECCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHH
AARLLGVGRATLYRKLRELDMSI
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQQGAETEQDLRARVPDERRATLAALELAATLSHFDQRLDRMEGVDSILRETAAKAQGL
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEMQSWAFFLVDPETGDFTCALTDGPEDRLRFEREVDALIEDRTFAWVLGRNRAVLVPSG
CEEECCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECC
IGAAGRLLLHPLATSSGLWGMFVAFTGEGAESTDLGFYLLTVVFFSCASMLESYSLYRQL
CCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QQVNEGLEQQVADRTRELVSINSSLEREVEERSLAEQQLRRTLNEKESYRQHLEAVFSSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDAIITVDLNGVVLNINAAAEKMLGIPAAQAVRMHHTALQLPCARACQDVLGATLGSGTP
HHEEEEEEECCEEEEECHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
VREFRAVSEDGAQVMILGGTPLISLDGTFSGAVLSCRDITRLVSLEQQLRQRHSFSNIIG
HHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSKVMQSLFGLLENLAAYDTTVLVTGESGTGKELVAEALHYNGARSGGPLVKVNCTALSE
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHHHH
SLLESELFGHVRGAFTGAVRDRAGRFETAEGGTIFLDEIGDISMRIQVLLLRFLESKEFE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
RVGDTTPRRADVRIVAATNADLHRRISEGTFRADLFYRLNVTSVHLPPLRERREDIPLLV
HHCCCCCCCCCEEEEEECCHHHHHHHHCCCEEEEEEEEEEEEEEECCCHHHHHCCCHHHH
QHFVEQCNTELGTRVAGVDEKAMAALVRAPWPGNVRELKHTLEHACVLCRDGYVEAGHLP
HHHHHHHCHHHCCEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCC
AELAAVESPPVRFDAAVSGPVSAAAGGAVPQPVAGGFGRRQLDARSITAAIEQAGGNKAR
HHHHHCCCCCCEEECCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHH
AARLLGVGRATLYRKLRELDMSI
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]