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The map label for this gene is fcl [H]

Identifier: 78355472

GI number: 78355472

Start: 423350

End: 424390

Strand: Direct

Name: fcl [H]

Synonym: Dde_0425

Alternate gene names: 78355472

Gene position: 423350-424390 (Clockwise)

Preceding gene: 78355471

Following gene: 78355473

Centisome position: 11.35

GC content: 60.23

Gene sequence:

>1041_bases
ATGACCATTGATATGGATGCTTCGATTTTTGTAGCCGGCCACCGGGGACTTGTGGGCAGCGCCATTGTCCGCTGTCTGCA
GGGGCACGGTGCACGCAATCTGCTGCTGAAGACTCATGCCGAGCTGGACCTGACCGACCAGCATGCCGTTGCCGGTTTTT
TTGCACGTCACCGGCCTCAGGTGGTGTTTCTGGCGGCGGCCAGAGTGGGCGGCATTCTGGCAAACGATACATATCCTGCT
GACTTTATACACATCAATCTGGCAATTCAGAACAATGTGCTGCATCAGGCGTATGTGCACGGGGCGCGCAGGCTGCTTTT
TCTGGGCAGTTCGTGCATTTACCCGCGGCTTGCTCCGCAGCCGCTGCGCGAAGAAAGCCTGATGACCGGACCGCTGGAGC
AGACCAATTCCGCCTATGCCGTGGCCAAAATAGCGGGCATTGAAATGTGCCGCGCCTATAACCGGCAATATGGTACGTCA
TTTGTGCCTGTCATGCCGACAAACCTTTACGGGCCGGGCGATTCCTTCAGTCTTGAGTCGTCGCATGTGCTGCCTGCGCT
GCTGCGCAAATGCCATCTGGCGCGGATGGTCATGCAGGGCGATATGCAGGCCGTGGAACGGGATGAGCAGGTGTTCGGTC
CGCTGCCCGCCGACGTGCGGACGCATCTGGGGCTGGACGGTGCGGGGACTGCGCCGCGCATCGCCGTGTGGGGATCGGGC
AATGCGCTTCGTGAGTTTCTGCATGTGGATGATCTGGCGGCGGCGTGCGTGCATCTGGTGTTCCGCACGCAGGAAACGGC
CCTTGTGAACATAGGTACGGGCGAAGACCTTACCATCCGTGAACTGGCGGAACTTGTCCGCAGTGTGGTGGGTGTGGATG
CGCCGCTGGTTTTTGATGCTGCAAAACCCGACGGCACACCGCGCAAGGTGCTTGACGTCTCGCGGATGCATTCGCTGGGC
TGGCGGCCTTCCATCGGGCTGCGTCAGGGCATTGCACAGGTGTACCGCTGGTATCTTGACCGGCTGGAAAGCGCGGCATG
A

Upstream 100 bases:

>100_bases
GGACCGGTTTTTGCTTGCCACAGGGGTGCGGCCGACGCAGCCGCGGCGCCGGGTGTGCGGACGTCATTTTTTTGGGCTTT
TGTATATGTAAGGGTATGTC

Downstream 100 bases:

>100_bases
GGCATGCCGCAGACGGGCTGCCGTGCAGGCCGCAACGGAGAATCCGATGACACAGGAATACGGCGGGCTGAAGCTGTCAG
TAATTATTCCCACGCATAAC

Product: GDP-fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTIDMDASIFVAGHRGLVGSAIVRCLQGHGARNLLLKTHAELDLTDQHAVAGFFARHRPQVVFLAAARVGGILANDTYPA
DFIHINLAIQNNVLHQAYVHGARRLLFLGSSCIYPRLAPQPLREESLMTGPLEQTNSAYAVAKIAGIEMCRAYNRQYGTS
FVPVMPTNLYGPGDSFSLESSHVLPALLRKCHLARMVMQGDMQAVERDEQVFGPLPADVRTHLGLDGAGTAPRIAVWGSG
NALREFLHVDDLAAACVHLVFRTQETALVNIGTGEDLTIRELAELVRSVVGVDAPLVFDAAKPDGTPRKVLDVSRMHSLG
WRPSIGLRQGIAQVYRWYLDRLESAA

Sequences:

>Translated_346_residues
MTIDMDASIFVAGHRGLVGSAIVRCLQGHGARNLLLKTHAELDLTDQHAVAGFFARHRPQVVFLAAARVGGILANDTYPA
DFIHINLAIQNNVLHQAYVHGARRLLFLGSSCIYPRLAPQPLREESLMTGPLEQTNSAYAVAKIAGIEMCRAYNRQYGTS
FVPVMPTNLYGPGDSFSLESSHVLPALLRKCHLARMVMQGDMQAVERDEQVFGPLPADVRTHLGLDGAGTAPRIAVWGSG
NALREFLHVDDLAAACVHLVFRTQETALVNIGTGEDLTIRELAELVRSVVGVDAPLVFDAAKPDGTPRKVLDVSRMHSLG
WRPSIGLRQGIAQVYRWYLDRLESAA
>Mature_345_residues
TIDMDASIFVAGHRGLVGSAIVRCLQGHGARNLLLKTHAELDLTDQHAVAGFFARHRPQVVFLAAARVGGILANDTYPAD
FIHINLAIQNNVLHQAYVHGARRLLFLGSSCIYPRLAPQPLREESLMTGPLEQTNSAYAVAKIAGIEMCRAYNRQYGTSF
VPVMPTNLYGPGDSFSLESSHVLPALLRKCHLARMVMQGDMQAVERDEQVFGPLPADVRTHLGLDGAGTAPRIAVWGSGN
ALREFLHVDDLAAACVHLVFRTQETALVNIGTGEDLTIRELAELVRSVVGVDAPLVFDAAKPDGTPRKVLDVSRMHSLGW
RPSIGLRQGIAQVYRWYLDRLESAA

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=345, Percent_Identity=30.1449275362319, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI1788365, Length=341, Percent_Identity=50.1466275659824, Blast_Score=342, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17554428, Length=346, Percent_Identity=26.3005780346821, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922778, Length=343, Percent_Identity=27.4052478134111, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 37743; Mature: 37612

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDMDASIFVAGHRGLVGSAIVRCLQGHGARNLLLKTHAELDLTDQHAVAGFFARHRPQ
CEEECCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCC
VVFLAAARVGGILANDTYPADFIHINLAIQNNVLHQAYVHGARRLLFLGSSCIYPRLAPQ
EEEEEEHHHCCEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHEEHCCCCCCCCCCCCC
PLREESLMTGPLEQTNSAYAVAKIAGIEMCRAYNRQYGTSFVPVMPTNLYGPGDSFSLES
CCCHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCEECEECCCCCCCCCCCCCCCC
SHVLPALLRKCHLARMVMQGDMQAVERDEQVFGPLPADVRTHLGLDGAGTAPRIAVWGSG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEECCC
NALREFLHVDDLAAACVHLVFRTQETALVNIGTGEDLTIRELAELVRSVVGVDAPLVFDA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEC
AKPDGTPRKVLDVSRMHSLGWRPSIGLRQGIAQVYRWYLDRLESAA
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIDMDASIFVAGHRGLVGSAIVRCLQGHGARNLLLKTHAELDLTDQHAVAGFFARHRPQ
EEECCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCC
VVFLAAARVGGILANDTYPADFIHINLAIQNNVLHQAYVHGARRLLFLGSSCIYPRLAPQ
EEEEEEHHHCCEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHEEHCCCCCCCCCCCCC
PLREESLMTGPLEQTNSAYAVAKIAGIEMCRAYNRQYGTSFVPVMPTNLYGPGDSFSLES
CCCHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCEECEECCCCCCCCCCCCCCCC
SHVLPALLRKCHLARMVMQGDMQAVERDEQVFGPLPADVRTHLGLDGAGTAPRIAVWGSG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEECCC
NALREFLHVDDLAAACVHLVFRTQETALVNIGTGEDLTIRELAELVRSVVGVDAPLVFDA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEC
AKPDGTPRKVLDVSRMHSLGWRPSIGLRQGIAQVYRWYLDRLESAA
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]