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The map label for this gene is ilvB [H]

Identifier: 78355445

GI number: 78355445

Start: 391085

End: 392902

Strand: Direct

Name: ilvB [H]

Synonym: Dde_0398

Alternate gene names: 78355445

Gene position: 391085-392902 (Clockwise)

Preceding gene: 78355444

Following gene: 78355446

Centisome position: 10.48

GC content: 62.38

Gene sequence:

>1818_bases
ATGAGCCGTTCCCGATCACGCGACCATGCCGCCGAAGCGCGGCCGTCTGCCGGAAAGGCTGCCATGCACGGCAGCAAAAC
GCAGTGTCCGGCGCCGGAAAAACAGCAGCCTGCCGCCCGGCCGGAGCGCATGACAGGTGCAGCAGCCGTTGTGCGGATGC
TTGAGCGACAGGGGGTGCGGCATGTGGCCGGCATTCCGGGCGGTGCCAGCCTGCCGCTGTACGATGCACTGGCAGCACGC
GGCAGCATCCGGCACATTCTCACCCGTCATGAACAGGCTGCCGGCTTCATTGCGCAGGGCATGGCCCGTGCCACCGGTGT
TCCTGCCGTATGTCTTGCCACTTCCGGTCCCGGCGCCACCAATATCCTTACCGCACTGGCCGATGCCAAGCTTGATTCGG
TTCCGGTTGTCTGCATCACGGGGCAGGTACCCACCACACTCATAGGTACCGATGCCTTTCAGGAGGTGGATACCTATGGC
ATGAGCATTCCTGTGACCAAACATAATTTTCTGGTCCGCACGGCGCAGGAACTGCTCTCGGTCATTCCCGAAGCATTCCG
CATTGCGGCTTCCGGCCGTCCTGGGCCGGTGCTTGTGGATATTCCGCGTGATGTGCAGCTGGCCACGCTGGAGATAGACA
GCTGGCCCGGACCGGGGCTGCCCGATGCTCCCCTGATGCCGGATATGGAAGACGTGGTGGGTGCCGCCGCCATGATAGAC
GAGGCGGAGCGTCCACTGATGTATCTGGGCGGCGGGGTCATCAGTTCCGGTGCCGCACCGGCGGCAAGGCGCATAGCCGA
AAAGGCGGGCATACCGGTGGCCATGACGCTTATGGGACTCGGGGCCGTTCCTTCGGAGCATCCTCTTTCACTCGGCATGC
TGGGCATGCACGCCGCGCGCTGCACCAACATGGCTATGGAAGAGTGCGACCTGCTTATTGTGGCGGGTGCCCGTTTTGAC
GACAGGGCCACAGGGCGTGTGGACAGCTTCTGCCCGCGGGCGCGGGTGATTCATATCGACATCGATGCCAGCGAACTGGA
TAAAATCCGCTCTGCCCATCTGGGTATTCAGGGCGATGTGCGGGCAACTCTGGAGGCGCTGGAGCCGCTGGTTGCCCCGT
GCGCCCGCGAAAACTGGCTGCACCGCGTGGAAGAGCTGCGCAACGGCTGGGGGCTGGATTTGCCGCGCACCGACGACCCC
GTAAGTCCTTACGGCATGATTTTACGGACGGCGGAAATAGTGGGTCCGGAAGCCGTGGTGACCACCGATGTGGGCCAGCA
TCAGATGCGCACGGCACAGGCATATCCTTTCAGCAGACCCCGGCAGTGGCTTACCTCCGGCGGGCTGGGCACCATGGGGT
TCGGTCTGCCCGCCGCCATCGGGGCCGCGCTTGCCTGTCCGGAAAGAACGGTGGTCTGCTTCACGGGTGACGGCAGCCTG
CAGATGAATATTCAGGAACTGGCCACCGCCGTGGAGCAGCGCTGCAACATCAAAATAATTCTTGCCAATAACAACGCTCT
GGGACTGGTGCAGCAGTTGCAGAATCTGTTTTTCGAGCGGCGGGTTTTCGCCACGGATTATTCGGTACAGGTTGATTTTG
TGCGTATTGCGGAAGGATTCGGCATGCCTGCCGTTGATCTGGGCGCCAGCCGTGATCCGCTGGGCATGCTGGAGGCCGCA
CTGACCACGGCGGGGCCTTGTCTTGTGAACATTCCCGTGGCGGTTTCGGAAAGTGTTTTTCCCATGGTGCCCCCCGGCGC
CGCCAACACAGAAATGATAGAAGGAGACGGCCATGACCGTACAGAAGCAGCAGCCTGA

Upstream 100 bases:

>100_bases
ACAGTATTCAGTCCAACCCCCGCCGGCGCAAGCCGGCGGGGGTTGTCTTGTTGCAGCCGGTCGCCGGAAGCCGCCCGGGG
AAAGCGAAGGAGGAGTCTCC

Downstream 100 bases:

>100_bases
CAGCGCCGCGGGGCTTATTGCCCTGCGCCTTACCGTGCGTAACCACCCCGGTGTCATGTCGCATGTGTGCGGTCTTTTTG
CCCGCCGCGCGTTCAACGTG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MSRSRSRDHAAEARPSAGKAAMHGSKTQCPAPEKQQPAARPERMTGAAAVVRMLERQGVRHVAGIPGGASLPLYDALAAR
GSIRHILTRHEQAAGFIAQGMARATGVPAVCLATSGPGATNILTALADAKLDSVPVVCITGQVPTTLIGTDAFQEVDTYG
MSIPVTKHNFLVRTAQELLSVIPEAFRIAASGRPGPVLVDIPRDVQLATLEIDSWPGPGLPDAPLMPDMEDVVGAAAMID
EAERPLMYLGGGVISSGAAPAARRIAEKAGIPVAMTLMGLGAVPSEHPLSLGMLGMHAARCTNMAMEECDLLIVAGARFD
DRATGRVDSFCPRARVIHIDIDASELDKIRSAHLGIQGDVRATLEALEPLVAPCARENWLHRVEELRNGWGLDLPRTDDP
VSPYGMILRTAEIVGPEAVVTTDVGQHQMRTAQAYPFSRPRQWLTSGGLGTMGFGLPAAIGAALACPERTVVCFTGDGSL
QMNIQELATAVEQRCNIKIILANNNALGLVQQLQNLFFERRVFATDYSVQVDFVRIAEGFGMPAVDLGASRDPLGMLEAA
LTTAGPCLVNIPVAVSESVFPMVPPGAANTEMIEGDGHDRTEAAA

Sequences:

>Translated_605_residues
MSRSRSRDHAAEARPSAGKAAMHGSKTQCPAPEKQQPAARPERMTGAAAVVRMLERQGVRHVAGIPGGASLPLYDALAAR
GSIRHILTRHEQAAGFIAQGMARATGVPAVCLATSGPGATNILTALADAKLDSVPVVCITGQVPTTLIGTDAFQEVDTYG
MSIPVTKHNFLVRTAQELLSVIPEAFRIAASGRPGPVLVDIPRDVQLATLEIDSWPGPGLPDAPLMPDMEDVVGAAAMID
EAERPLMYLGGGVISSGAAPAARRIAEKAGIPVAMTLMGLGAVPSEHPLSLGMLGMHAARCTNMAMEECDLLIVAGARFD
DRATGRVDSFCPRARVIHIDIDASELDKIRSAHLGIQGDVRATLEALEPLVAPCARENWLHRVEELRNGWGLDLPRTDDP
VSPYGMILRTAEIVGPEAVVTTDVGQHQMRTAQAYPFSRPRQWLTSGGLGTMGFGLPAAIGAALACPERTVVCFTGDGSL
QMNIQELATAVEQRCNIKIILANNNALGLVQQLQNLFFERRVFATDYSVQVDFVRIAEGFGMPAVDLGASRDPLGMLEAA
LTTAGPCLVNIPVAVSESVFPMVPPGAANTEMIEGDGHDRTEAAA
>Mature_604_residues
SRSRSRDHAAEARPSAGKAAMHGSKTQCPAPEKQQPAARPERMTGAAAVVRMLERQGVRHVAGIPGGASLPLYDALAARG
SIRHILTRHEQAAGFIAQGMARATGVPAVCLATSGPGATNILTALADAKLDSVPVVCITGQVPTTLIGTDAFQEVDTYGM
SIPVTKHNFLVRTAQELLSVIPEAFRIAASGRPGPVLVDIPRDVQLATLEIDSWPGPGLPDAPLMPDMEDVVGAAAMIDE
AERPLMYLGGGVISSGAAPAARRIAEKAGIPVAMTLMGLGAVPSEHPLSLGMLGMHAARCTNMAMEECDLLIVAGARFDD
RATGRVDSFCPRARVIHIDIDASELDKIRSAHLGIQGDVRATLEALEPLVAPCARENWLHRVEELRNGWGLDLPRTDDPV
SPYGMILRTAEIVGPEAVVTTDVGQHQMRTAQAYPFSRPRQWLTSGGLGTMGFGLPAAIGAALACPERTVVCFTGDGSLQ
MNIQELATAVEQRCNIKIILANNNALGLVQQLQNLFFERRVFATDYSVQVDFVRIAEGFGMPAVDLGASRDPLGMLEAAL
TTAGPCLVNIPVAVSESVFPMVPPGAANTEMIEGDGHDRTEAAA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=490, Percent_Identity=27.7551020408163, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI21361361, Length=511, Percent_Identity=27.2015655577299, Blast_Score=149, Evalue=8e-36,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=58.7703435804702, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=42.9328621908127, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1786717, Length=535, Percent_Identity=34.9532710280374, Blast_Score=308, Evalue=1e-84,
Organism=Escherichia coli, GI1787096, Length=554, Percent_Identity=28.5198555956679, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1788716, Length=480, Percent_Identity=26.4583333333333, Blast_Score=139, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=542, Percent_Identity=25.4612546125461, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=481, Percent_Identity=28.0665280665281, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=26.9090909090909, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=38.8316151202749, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6320816, Length=487, Percent_Identity=25.4620123203285, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19922626, Length=568, Percent_Identity=27.6408450704225, Blast_Score=176, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63867; Mature: 63736

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSRSRDHAAEARPSAGKAAMHGSKTQCPAPEKQQPAARPERMTGAAAVVRMLERQGVR
CCCCCCCCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
HVAGIPGGASLPLYDALAARGSIRHILTRHEQAAGFIAQGMARATGVPAVCLATSGPGAT
EECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
NILTALADAKLDSVPVVCITGQVPTTLIGTDAFQEVDTYGMSIPVTKHNFLVRTAQELLS
HHHHHHHHCCCCCCCEEEEECCCCCEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VIPEAFRIAASGRPGPVLVDIPRDVQLATLEIDSWPGPGLPDAPLMPDMEDVVGAAAMID
HHHHHHEECCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
EAERPLMYLGGGVISSGAAPAARRIAEKAGIPVAMTLMGLGAVPSEHPLSLGMLGMHAAR
HCCCCEEEECCCHHCCCCCHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCHHHHHHHHHH
CTNMAMEECDLLIVAGARFDDRATGRVDSFCPRARVIHIDIDASELDKIRSAHLGIQGDV
HHHHHHHHCCEEEEECCCCCCCCCCCHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCHH
RATLEALEPLVAPCARENWLHRVEELRNGWGLDLPRTDDPVSPYGMILRTAEIVGPEAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
TTDVGQHQMRTAQAYPFSRPRQWLTSGGLGTMGFGLPAAIGAALACPERTVVCFTGDGSL
EECCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCE
QMNIQELATAVEQRCNIKIILANNNALGLVQQLQNLFFERRVFATDYSVQVDFVRIAEGF
EEEHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCEEEEEEEEEECCC
GMPAVDLGASRDPLGMLEAALTTAGPCLVNIPVAVSESVFPMVPPGAANTEMIEGDGHDR
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCCCCCCCEEECCCCCC
TEAAA
CCCCC
>Mature Secondary Structure 
SRSRSRDHAAEARPSAGKAAMHGSKTQCPAPEKQQPAARPERMTGAAAVVRMLERQGVR
CCCCCCCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
HVAGIPGGASLPLYDALAARGSIRHILTRHEQAAGFIAQGMARATGVPAVCLATSGPGAT
EECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
NILTALADAKLDSVPVVCITGQVPTTLIGTDAFQEVDTYGMSIPVTKHNFLVRTAQELLS
HHHHHHHHCCCCCCCEEEEECCCCCEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VIPEAFRIAASGRPGPVLVDIPRDVQLATLEIDSWPGPGLPDAPLMPDMEDVVGAAAMID
HHHHHHEECCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
EAERPLMYLGGGVISSGAAPAARRIAEKAGIPVAMTLMGLGAVPSEHPLSLGMLGMHAAR
HCCCCEEEECCCHHCCCCCHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCHHHHHHHHHH
CTNMAMEECDLLIVAGARFDDRATGRVDSFCPRARVIHIDIDASELDKIRSAHLGIQGDV
HHHHHHHHCCEEEEECCCCCCCCCCCHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCHH
RATLEALEPLVAPCARENWLHRVEELRNGWGLDLPRTDDPVSPYGMILRTAEIVGPEAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
TTDVGQHQMRTAQAYPFSRPRQWLTSGGLGTMGFGLPAAIGAALACPERTVVCFTGDGSL
EECCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCE
QMNIQELATAVEQRCNIKIILANNNALGLVQQLQNLFFERRVFATDYSVQVDFVRIAEGF
EEEHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCEEEEEEEEEECCC
GMPAVDLGASRDPLGMLEAALTTAGPCLVNIPVAVSESVFPMVPPGAANTEMIEGDGHDR
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCCCCCCCEEECCCCCC
TEAAA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]