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The map label for this gene is braC [H]

Identifier: 78355305

GI number: 78355305

Start: 242466

End: 243647

Strand: Direct

Name: braC [H]

Synonym: Dde_0258

Alternate gene names: 78355305

Gene position: 242466-243647 (Clockwise)

Preceding gene: 304570576

Following gene: 78355306

Centisome position: 6.5

GC content: 58.46

Gene sequence:

>1182_bases
ATGGCATCAGGCCGCGTACGCATGACGCCCGCATACCCGCGGGCAGGAGGTTTTTTCATGAGCAGAAAAATGACGGCTTT
TGTTCTGGGTGTTGTTGCCACGGTGCTGATGGCCGGCACTGCCATGGCCGGTACCGTTAAAATCGGTCTTATGTGTCCGC
TGACCGGTTCGTGGGCCAGCGAAGGTCAGGACATGCGCAACATTGTTCTGATGCTGGCCGAACAGACCAACGCTCAGGGC
GGCATTAACGGCGACATGGTGGAGATAGTGGTCGAGGACGATGCCGGGGACCCCAAAACTGCCGCACTGGCAGCCCAGCG
TCTTTCCACCATGGGTGTTTCGGCCGTCATCGGCTCATACGGTTCGGCCATTACCGAAGCCTCGCAGGCCATCTATGACG
AGATGGACATCGTGCAGGTGGCTACCGGTTCCACGGCCATCCGGCTTACCGAAAAAGGTCTGCCGTTGTTTTTCCGCACC
TGTCCGCGCGACGACGAACAGGGCCGCGTGGCCGCAGCCGTGCTGAAGGAAAAAGGCTATAAAAAGATCGCCATCCTGCA
CGACAACACGTCGTATGCCAAAGGGTTGGCCGAAGAAACGCTGACTCTGCTGAAAAGCGCAGGCACCGAAGTGGTGTTCT
ATGATGCGCTCACTCCCAACGAACGTGATTATTCCGCAATACTTACCAAGCTGAAGGCCGCAGGGCCCGACGCCATATTC
TTTACCGGCTACTACCCCGAGGCAGGCATGCTGCTGCGGCAGAAGCAGGAAATGAAGTGGGACGTGCCCATGATGGGCGG
CGACGCCACCAACAACCTTGATCTGGTGAAAATTGCGGGCAAATCCGCTGCCGGATTCCTGTTCATCAGCCCGCCCACCC
TGCAGGACCTGCCCGACAAGCAGGCAAAGGACTTTGCCGCCGCGTACAAGGCCCGCTACGGTACCATGCCTGCTTCCGTG
TGGTCTGTGCTGGCCGGCGATGCCTTTATCGCTCTGAAGGCCGCCATCACCGGTGCCGGTTCCACCGACCCGCAGGATAT
TGCCGCGTACATGAAAAAAGATCTCAAGGATCTGCCGGGTCTTACCGGTTCGCTTTCGTTCAACGAAAAAGGCGACCGTG
TGGGCGACCTGTACCGCCTGTACCGCGTGGATGAAAACGGCAACTTTGTACTGCAGCCGTAA

Upstream 100 bases:

>100_bases
TCTGTCTGGCCGGTATATGCTCCTCACGGGATACCCGTCTCCGCAGACGCATACGGCGCACGGAGGCTCCGGCGGCAACA
GTGGCGGACGTTGAGGAATG

Downstream 100 bases:

>100_bases
CCACCTTACCCCATTGGCAAACACGGACGGGGGGTGTATGCTCCCCGTCCTGCCGCATAGCCGGATCATGCCGGTCAGAC
CGGTGTGACGCACCGCACTT

Product: amino acid ABC transporter periplasmic-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MASGRVRMTPAYPRAGGFFMSRKMTAFVLGVVATVLMAGTAMAGTVKIGLMCPLTGSWASEGQDMRNIVLMLAEQTNAQG
GINGDMVEIVVEDDAGDPKTAALAAQRLSTMGVSAVIGSYGSAITEASQAIYDEMDIVQVATGSTAIRLTEKGLPLFFRT
CPRDDEQGRVAAAVLKEKGYKKIAILHDNTSYAKGLAEETLTLLKSAGTEVVFYDALTPNERDYSAILTKLKAAGPDAIF
FTGYYPEAGMLLRQKQEMKWDVPMMGGDATNNLDLVKIAGKSAAGFLFISPPTLQDLPDKQAKDFAAAYKARYGTMPASV
WSVLAGDAFIALKAAITGAGSTDPQDIAAYMKKDLKDLPGLTGSLSFNEKGDRVGDLYRLYRVDENGNFVLQP

Sequences:

>Translated_393_residues
MASGRVRMTPAYPRAGGFFMSRKMTAFVLGVVATVLMAGTAMAGTVKIGLMCPLTGSWASEGQDMRNIVLMLAEQTNAQG
GINGDMVEIVVEDDAGDPKTAALAAQRLSTMGVSAVIGSYGSAITEASQAIYDEMDIVQVATGSTAIRLTEKGLPLFFRT
CPRDDEQGRVAAAVLKEKGYKKIAILHDNTSYAKGLAEETLTLLKSAGTEVVFYDALTPNERDYSAILTKLKAAGPDAIF
FTGYYPEAGMLLRQKQEMKWDVPMMGGDATNNLDLVKIAGKSAAGFLFISPPTLQDLPDKQAKDFAAAYKARYGTMPASV
WSVLAGDAFIALKAAITGAGSTDPQDIAAYMKKDLKDLPGLTGSLSFNEKGDRVGDLYRLYRVDENGNFVLQP
>Mature_392_residues
ASGRVRMTPAYPRAGGFFMSRKMTAFVLGVVATVLMAGTAMAGTVKIGLMCPLTGSWASEGQDMRNIVLMLAEQTNAQGG
INGDMVEIVVEDDAGDPKTAALAAQRLSTMGVSAVIGSYGSAITEASQAIYDEMDIVQVATGSTAIRLTEKGLPLFFRTC
PRDDEQGRVAAAVLKEKGYKKIAILHDNTSYAKGLAEETLTLLKSAGTEVVFYDALTPNERDYSAILTKLKAAGPDAIFF
TGYYPEAGMLLRQKQEMKWDVPMMGGDATNNLDLVKIAGKSAAGFLFISPPTLQDLPDKQAKDFAAAYKARYGTMPASVW
SVLAGDAFIALKAAITGAGSTDPQDIAAYMKKDLKDLPGLTGSLSFNEKGDRVGDLYRLYRVDENGNFVLQP

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=370, Percent_Identity=32.972972972973, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789867, Length=370, Percent_Identity=31.6216216216216, Blast_Score=171, Evalue=6e-44,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41958; Mature: 41826

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGRVRMTPAYPRAGGFFMSRKMTAFVLGVVATVLMAGTAMAGTVKIGLMCPLTGSWAS
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECEEEEEEECCCCCCHHH
EGQDMRNIVLMLAEQTNAQGGINGDMVEIVVEDDAGDPKTAALAAQRLSTMGVSAVIGSY
CCHHHHHHHEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
GSAITEASQAIYDEMDIVQVATGSTAIRLTEKGLPLFFRTCPRDDEQGRVAAAVLKEKGY
HHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHCCC
KKIAILHDNTSYAKGLAEETLTLLKSAGTEVVFYDALTPNERDYSAILTKLKAAGPDAIF
EEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEE
FTGYYPEAGMLLRQKQEMKWDVPMMGGDATNNLDLVKIAGKSAAGFLFISPPTLQDLPDK
EEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHCCCCH
QAKDFAAAYKARYGTMPASVWSVLAGDAFIALKAAITGAGSTDPQDIAAYMKKDLKDLPG
HHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
LTGSLSFNEKGDRVGDLYRLYRVDENGNFVLQP
CCCEECCCCCCCHHHHEEEEEEECCCCCEEECC
>Mature Secondary Structure 
ASGRVRMTPAYPRAGGFFMSRKMTAFVLGVVATVLMAGTAMAGTVKIGLMCPLTGSWAS
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECEEEEEEECCCCCCHHH
EGQDMRNIVLMLAEQTNAQGGINGDMVEIVVEDDAGDPKTAALAAQRLSTMGVSAVIGSY
CCHHHHHHHEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
GSAITEASQAIYDEMDIVQVATGSTAIRLTEKGLPLFFRTCPRDDEQGRVAAAVLKEKGY
HHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHCCC
KKIAILHDNTSYAKGLAEETLTLLKSAGTEVVFYDALTPNERDYSAILTKLKAAGPDAIF
EEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEE
FTGYYPEAGMLLRQKQEMKWDVPMMGGDATNNLDLVKIAGKSAAGFLFISPPTLQDLPDK
EEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHCCCCH
QAKDFAAAYKARYGTMPASVWSVLAGDAFIALKAAITGAGSTDPQDIAAYMKKDLKDLPG
HHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
LTGSLSFNEKGDRVGDLYRLYRVDENGNFVLQP
CCCEECCCCCCCHHHHEEEEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]