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The map label for this gene is ilvD

Identifier: 78355163

GI number: 78355163

Start: 108514

End: 110181

Strand: Direct

Name: ilvD

Synonym: Dde_0116

Alternate gene names: 78355163

Gene position: 108514-110181 (Clockwise)

Preceding gene: 78355162

Following gene: 78355166

Centisome position: 2.91

GC content: 60.37

Gene sequence:

>1668_bases
ATGAGAAGCAAGAAAATGACACACGGGCTTGAAAAAGCCCCGCATCGTTCGCTGCTGTACGCCCTGGGCCTGACCCGTGA
AGAATTGTCCAGACCGCTTGTGGGGGTGGTCAATTCCGCCAACGAGGTGGTACCGGGACACATCCACCTGCACACCATTG
CCGAAGCAGTGAAGGCCGGAGTGCGCGCCGCGGGTGGCACACCCATGGAGTTTCCCGCCATAGCGGTATGCGACGGGCTG
GCCATGAACCATGAAGGCATGCGTTTTTCGTTGCCCAGCCGCGAAATCATTGCAGATTCCATTGAGATAATGGCCACGGC
TCATCCTTTTGATGCGCTGGTCTTCATACCCAACTGTGATAAGTCGGTTCCCGGCATGCTTATGGCCATGCTGCGTATGA
ACGTGCCGTCCATTCTTATCAGCGGCGGCCCCATGATGGCAGGCACGGGCAACGGCAAGGCCGCAGACCTCATCACCGTA
TTTGAAGGTGTGGGCATGGTGCGGCAGGGCAGAATGACGGAAGACGAACTGGAGCAGCTTTCCGAGACGGCATGTCCCGG
CTGCGGGTCGTGCGCGGGCATGTTCACGGCAAACTCCATGAACTGTCTTTCCGAAACCATAGGGCTTGCGCTGCCGGGCA
ACGGTACCACGCCGGCCAATTCCGCAGCGCGGGTGCGGCTGGCCAAACATGCCGGCATGCGCGTCATGGAGCTGCTGGAG
CGCGATATAAAGCCGCGTGATATCGTGACCGAAAAGGCCGTGGCCAACGCTGTTGCCGCGGATATGGCGCTGGGCTGTTC
CACAAACACGGTACTGCATCTGCCCGCTGTATTTGCCGAGGCAGGGCTTGATCTTACTCTGGGCATTTTTGACGAGGTAA
GCCGCAAGACCCCCAACCTGTGCAAGCTCTCGCCCGCCGGTGACCAGCATATACAGGACCTGCACCGTGCAGGGGGTATT
CCCGCGGTCATGGCCGAACTGGACAAGGCCGGGCGCATACACCGCGACGCACTGACCGTCACAGGCCGTACCGTGGGCGA
AAACCTTGATGCACTGGGCGCCGCGGTGCGTGACCACGATGTCATCAGGCCCATGGAAAAGCCGTATTCGCCGCAGGGCG
GTATTGCCATTCTGTACGGTTCGCTTGCGCCGGAAGGGGCCGTGGTCAAGCAGTCTGCCGTTGCACCGGAAATGATGCAG
CGTACAGCGCGTGCCCGTGTGTTTGAATCAGAAGAAGCTGCCTTTGAAGCCATTATGGCGCAGCGGATTGAAAAGGGCGA
TGCCATCATCATACGCTACGAAGGGCCGAAAGGCGGTCCCGGTATGCGCGAAATGCTGTCTCCCACCGCAGCCATTGCCG
GCATAGGGCTGGGGGCCGATGTGGCGTTGATTACCGACGGGCGTTTTTCCGGCGGAACCAGAGGTGCCGCCATAGGGCAT
GTATCACCCGAAGCTGCTGACGGCGGTCCCATAGGGCTGGTGCGTGAAGGGGACATGATCGAGATAGACATCCCCGCCCG
CAAGCTGGACCTGCTGGTTGACGAAGCGGAAATGGAGCGCCGGCGTGCTGCATTTACGCCCGTGGTGAAAGAGGTCACCT
CTCCGCTGCTGAAACGCTACAGCCGTCTGGTGACGTCTGCCGCCAACGGTGCCGTGTACAAGCGCTGA

Upstream 100 bases:

>100_bases
ATTCAGTGCGTTGCCGCAGGGTTTCCGGTAGCTTTCCGGCGGGTGTCTTTCCTTCCGGCCGGCTGCGCGGGCTTTGACGT
CAGCGCGCACAGAGGACAAC

Downstream 100 bases:

>100_bases
CCCGTTTTTCTGTCCTGAATGACCCCGGGCACCGGCATAGTTCCGGTGCCCGTTTTTTTATGCATCACAGTTCCGAAACA
GAAAGAGCCTCCGGCAGGGC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MRSKKMTHGLEKAPHRSLLYALGLTREELSRPLVGVVNSANEVVPGHIHLHTIAEAVKAGVRAAGGTPMEFPAIAVCDGL
AMNHEGMRFSLPSREIIADSIEIMATAHPFDALVFIPNCDKSVPGMLMAMLRMNVPSILISGGPMMAGTGNGKAADLITV
FEGVGMVRQGRMTEDELEQLSETACPGCGSCAGMFTANSMNCLSETIGLALPGNGTTPANSAARVRLAKHAGMRVMELLE
RDIKPRDIVTEKAVANAVAADMALGCSTNTVLHLPAVFAEAGLDLTLGIFDEVSRKTPNLCKLSPAGDQHIQDLHRAGGI
PAVMAELDKAGRIHRDALTVTGRTVGENLDALGAAVRDHDVIRPMEKPYSPQGGIAILYGSLAPEGAVVKQSAVAPEMMQ
RTARARVFESEEAAFEAIMAQRIEKGDAIIIRYEGPKGGPGMREMLSPTAAIAGIGLGADVALITDGRFSGGTRGAAIGH
VSPEAADGGPIGLVREGDMIEIDIPARKLDLLVDEAEMERRRAAFTPVVKEVTSPLLKRYSRLVTSAANGAVYKR

Sequences:

>Translated_555_residues
MRSKKMTHGLEKAPHRSLLYALGLTREELSRPLVGVVNSANEVVPGHIHLHTIAEAVKAGVRAAGGTPMEFPAIAVCDGL
AMNHEGMRFSLPSREIIADSIEIMATAHPFDALVFIPNCDKSVPGMLMAMLRMNVPSILISGGPMMAGTGNGKAADLITV
FEGVGMVRQGRMTEDELEQLSETACPGCGSCAGMFTANSMNCLSETIGLALPGNGTTPANSAARVRLAKHAGMRVMELLE
RDIKPRDIVTEKAVANAVAADMALGCSTNTVLHLPAVFAEAGLDLTLGIFDEVSRKTPNLCKLSPAGDQHIQDLHRAGGI
PAVMAELDKAGRIHRDALTVTGRTVGENLDALGAAVRDHDVIRPMEKPYSPQGGIAILYGSLAPEGAVVKQSAVAPEMMQ
RTARARVFESEEAAFEAIMAQRIEKGDAIIIRYEGPKGGPGMREMLSPTAAIAGIGLGADVALITDGRFSGGTRGAAIGH
VSPEAADGGPIGLVREGDMIEIDIPARKLDLLVDEAEMERRRAAFTPVVKEVTSPLLKRYSRLVTSAANGAVYKR
>Mature_555_residues
MRSKKMTHGLEKAPHRSLLYALGLTREELSRPLVGVVNSANEVVPGHIHLHTIAEAVKAGVRAAGGTPMEFPAIAVCDGL
AMNHEGMRFSLPSREIIADSIEIMATAHPFDALVFIPNCDKSVPGMLMAMLRMNVPSILISGGPMMAGTGNGKAADLITV
FEGVGMVRQGRMTEDELEQLSETACPGCGSCAGMFTANSMNCLSETIGLALPGNGTTPANSAARVRLAKHAGMRVMELLE
RDIKPRDIVTEKAVANAVAADMALGCSTNTVLHLPAVFAEAGLDLTLGIFDEVSRKTPNLCKLSPAGDQHIQDLHRAGGI
PAVMAELDKAGRIHRDALTVTGRTVGENLDALGAAVRDHDVIRPMEKPYSPQGGIAILYGSLAPEGAVVKQSAVAPEMMQ
RTARARVFESEEAAFEAIMAQRIEKGDAIIIRYEGPKGGPGMREMLSPTAAIAGIGLGADVALITDGRFSGGTRGAAIGH
VSPEAADGGPIGLVREGDMIEIDIPARKLDLLVDEAEMERRRAAFTPVVKEVTSPLLKRYSRLVTSAANGAVYKR

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=609, Percent_Identity=44.8275862068966, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1786464, Length=494, Percent_Identity=34.8178137651822, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI2367371, Length=499, Percent_Identity=32.2645290581162, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI1788157, Length=523, Percent_Identity=32.5047801147228, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6322476, Length=545, Percent_Identity=42.0183486238532, Blast_Score=421, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_DESDG (Q317H9)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_386612.1
- STRING:   Q317H9
- GeneID:   3756434
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0116
- NMPDR:   fig|207559.3.peg.772
- eggNOG:   COG0129
- HOGENOM:   HBG671001
- OMA:   QSERWES
- ProtClustDB:   PRK00911
- BioCyc:   DDES207559:DDE_0116-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 58579; Mature: 58579

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKKMTHGLEKAPHRSLLYALGLTREELSRPLVGVVNSANEVVPGHIHLHTIAEAVKAG
CCCCHHHHHHHHCCHHHHHHHHCCCHHHHCCCHHHHHCCCCCCCCCEEHHHHHHHHHHHH
VRAAGGTPMEFPAIAVCDGLAMNHEGMRFSLPSREIIADSIEIMATAHPFDALVFIPNCD
HHCCCCCCCCCCHHHHHCCCEECCCCCEEECCCHHHHHHHHHEEEECCCCEEEEECCCCC
KSVPGMLMAMLRMNVPSILISGGPMMAGTGNGKAADLITVFEGVGMVRQGRMTEDELEQL
CCHHHHHHHHHHCCCCEEEEECCCEEEECCCCCHHHHHHHHHHCCHHHCCCCCHHHHHHH
SETACPGCGSCAGMFTANSMNCLSETIGLALPGNGTTPANSAARVRLAKHAGMRVMELLE
HHHCCCCCCCCHHHHCCCHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHH
RDIKPRDIVTEKAVANAVAADMALGCSTNTVLHLPAVFAEAGLDLTLGIFDEVSRKTPNL
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHCCCCEEEHHHHHHHHCCCCC
CKLSPAGDQHIQDLHRAGGIPAVMAELDKAGRIHRDALTVTGRTVGENLDALGAAVRDHD
EEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEHHEEEEECCHHHCCHHHHHHHHHHHH
VIRPMEKPYSPQGGIAILYGSLAPEGAVVKQSAVAPEMMQRTARARVFESEEAAFEAIMA
HHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHH
QRIEKGDAIIIRYEGPKGGPGMREMLSPTAAIAGIGLGADVALITDGRFSGGTRGAAIGH
HHHCCCCEEEEEECCCCCCCCHHHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCCEECC
VSPEAADGGPIGLVREGDMIEIDIPARKLDLLVDEAEMERRRAAFTPVVKEVTSPLLKRY
CCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLVTSAANGAVYKR
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MRSKKMTHGLEKAPHRSLLYALGLTREELSRPLVGVVNSANEVVPGHIHLHTIAEAVKAG
CCCCHHHHHHHHCCHHHHHHHHCCCHHHHCCCHHHHHCCCCCCCCCEEHHHHHHHHHHHH
VRAAGGTPMEFPAIAVCDGLAMNHEGMRFSLPSREIIADSIEIMATAHPFDALVFIPNCD
HHCCCCCCCCCCHHHHHCCCEECCCCCEEECCCHHHHHHHHHEEEECCCCEEEEECCCCC
KSVPGMLMAMLRMNVPSILISGGPMMAGTGNGKAADLITVFEGVGMVRQGRMTEDELEQL
CCHHHHHHHHHHCCCCEEEEECCCEEEECCCCCHHHHHHHHHHCCHHHCCCCCHHHHHHH
SETACPGCGSCAGMFTANSMNCLSETIGLALPGNGTTPANSAARVRLAKHAGMRVMELLE
HHHCCCCCCCCHHHHCCCHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHH
RDIKPRDIVTEKAVANAVAADMALGCSTNTVLHLPAVFAEAGLDLTLGIFDEVSRKTPNL
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHCCCCEEEHHHHHHHHCCCCC
CKLSPAGDQHIQDLHRAGGIPAVMAELDKAGRIHRDALTVTGRTVGENLDALGAAVRDHD
EEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEHHEEEEECCHHHCCHHHHHHHHHHHH
VIRPMEKPYSPQGGIAILYGSLAPEGAVVKQSAVAPEMMQRTARARVFESEEAAFEAIMA
HHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHH
QRIEKGDAIIIRYEGPKGGPGMREMLSPTAAIAGIGLGADVALITDGRFSGGTRGAAIGH
HHHCCCCEEEEEECCCCCCCCHHHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCCEECC
VSPEAADGGPIGLVREGDMIEIDIPARKLDLLVDEAEMERRRAAFTPVVKEVTSPLLKRY
CCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLVTSAANGAVYKR
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA