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The map label for this gene is glnA [H]

Identifier: 78355151

GI number: 78355151

Start: 93668

End: 95017

Strand: Direct

Name: glnA [H]

Synonym: Dde_0104

Alternate gene names: 78355151

Gene position: 93668-95017 (Clockwise)

Preceding gene: 78355149

Following gene: 78355152

Centisome position: 2.51

GC content: 55.41

Gene sequence:

>1350_bases
ATGAGTACAAACTATCCCGTCTTCAACTGCAAAAACGGCGATGATGTCATCAGAGCCGTGAAGGACTACAATGTCAGCTT
TGTGCAGTTCTGGTTTGTGGATATTCTGGGCAACCTCAAAAGTTTTCAGGTAACCCCTGACGAACTGGAAGCAGCCTTTG
AAGAAGGAATGGGGTTTGACGGCTCCTCAATTCTCGGGTTCACCCGCATTGAAGAAAGCGACATGATCGCGTTTCCTGAC
GCTTCAAGCTTTCAGATATGCTCGTGGCGTCCCATGGACCGCCCCGTGGCCCGCATGTTCTGCGATATCCGCACGCCCGA
CGGCAACCCCTACGAAGGCGACCCCAGATATGTGCTGCGCAGGCTGGTGGATAAGGCCGCACAGAAGGGGTACACCTATT
ATGTAGGCCCGGAACTGGAATTTTTCCTTTTCGCTGACTCCACATGCCCCAGACCTCTTGATTCCGGAGGGTACTTTGAC
GCGCCCCCGCTGGATCTGGGCAATGAAGTGCGCCGTGACATTATTTTTGCCCTGCAGAAAATGGGCATAGATGTGGAATA
CAGCCATCATGAGGTGGCTCCTTCGCAGCACGAAATAGACCTGCGGTACAACGAAGCCCTGAAAATGGCTGATATAGCCA
TGACCTACAAGGTTGTGGTCAAAGAGATTGCCCGTAAACACGGCGTGTACGCCACGTTTATGCCCAAGCCCATTTTCGGC
GAAAACGGCTCGGGCATGCATGTGCACCAGTCTCTTTTCAAGAACGGTAAAAACGCTTTCTTTGATGCCAACGACCCGCA
TCACCTGTCGGCCGAAGCCCGCCATTACATCGCCGGTCTGCTGACCCATGCCAGTGAGATTACCTGCATCACTAACCAGT
GGGTGAACTCATACAAGCGGCTGGTGCCCGGATATGAGGCCCCGGTGTATATCGCGTGGGCGCAGCGCAACCGTTCGTCG
CTTATCCGTGTGCCCATGTACAAACCCGGAAAGGAAGCGGCAACCCGTATCGAGCTGCGCAACCCCGACCCCGCCTGCAA
CCCGTACCTTGCCTTTGCCGCCATGCTGGGCGCAGGTCTGGAAGGCATAGAAAAAGGGTACGAAGTGCCGCGCGCGGTGG
AAGAGAATATCTTTGCCATGGAGGCCGAAGACCTGAGCGCCAAGGGCATTGATTCGCTGCCCGGTTCGCTGTACGAGGCC
GCGGTGGCCCTGCGGGACAGCGCCCTGATGAAAGAAGTGCTGGGCGAACATACGCACCAGAATCTTGTGGGCAACAAGCT
CATTGAATGGGATGCCTACCGCACCCATGTTTCCGAGTTTGAGCTTAAGCGGTATCTGCCGGTCCTGTAG

Upstream 100 bases:

>100_bases
GCCGCCATGCCCGTCCGCTTGCGGGGCGGAACGTCGTGTCTGCCTCCTCAGCGGCGCCTTGTCTCATGCACCGCAACCAA
CCACCGAGAGGAAATAGCGT

Downstream 100 bases:

>100_bases
CCGGTGCACCGTCTGCCTCTCCGTGGCGGCGGTTTACGCGTAATGACAAACCCCGTCTTTTCCGGACGGGGTTTTATTTT
CGGTAATGCCAGCCGCAGAG

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSTNYPVFNCKNGDDVIRAVKDYNVSFVQFWFVDILGNLKSFQVTPDELEAAFEEGMGFDGSSILGFTRIEESDMIAFPD
ASSFQICSWRPMDRPVARMFCDIRTPDGNPYEGDPRYVLRRLVDKAAQKGYTYYVGPELEFFLFADSTCPRPLDSGGYFD
APPLDLGNEVRRDIIFALQKMGIDVEYSHHEVAPSQHEIDLRYNEALKMADIAMTYKVVVKEIARKHGVYATFMPKPIFG
ENGSGMHVHQSLFKNGKNAFFDANDPHHLSAEARHYIAGLLTHASEITCITNQWVNSYKRLVPGYEAPVYIAWAQRNRSS
LIRVPMYKPGKEAATRIELRNPDPACNPYLAFAAMLGAGLEGIEKGYEVPRAVEENIFAMEAEDLSAKGIDSLPGSLYEA
AVALRDSALMKEVLGEHTHQNLVGNKLIEWDAYRTHVSEFELKRYLPVL

Sequences:

>Translated_449_residues
MSTNYPVFNCKNGDDVIRAVKDYNVSFVQFWFVDILGNLKSFQVTPDELEAAFEEGMGFDGSSILGFTRIEESDMIAFPD
ASSFQICSWRPMDRPVARMFCDIRTPDGNPYEGDPRYVLRRLVDKAAQKGYTYYVGPELEFFLFADSTCPRPLDSGGYFD
APPLDLGNEVRRDIIFALQKMGIDVEYSHHEVAPSQHEIDLRYNEALKMADIAMTYKVVVKEIARKHGVYATFMPKPIFG
ENGSGMHVHQSLFKNGKNAFFDANDPHHLSAEARHYIAGLLTHASEITCITNQWVNSYKRLVPGYEAPVYIAWAQRNRSS
LIRVPMYKPGKEAATRIELRNPDPACNPYLAFAAMLGAGLEGIEKGYEVPRAVEENIFAMEAEDLSAKGIDSLPGSLYEA
AVALRDSALMKEVLGEHTHQNLVGNKLIEWDAYRTHVSEFELKRYLPVL
>Mature_448_residues
STNYPVFNCKNGDDVIRAVKDYNVSFVQFWFVDILGNLKSFQVTPDELEAAFEEGMGFDGSSILGFTRIEESDMIAFPDA
SSFQICSWRPMDRPVARMFCDIRTPDGNPYEGDPRYVLRRLVDKAAQKGYTYYVGPELEFFLFADSTCPRPLDSGGYFDA
PPLDLGNEVRRDIIFALQKMGIDVEYSHHEVAPSQHEIDLRYNEALKMADIAMTYKVVVKEIARKHGVYATFMPKPIFGE
NGSGMHVHQSLFKNGKNAFFDANDPHHLSAEARHYIAGLLTHASEITCITNQWVNSYKRLVPGYEAPVYIAWAQRNRSSL
IRVPMYKPGKEAATRIELRNPDPACNPYLAFAAMLGAGLEGIEKGYEVPRAVEENIFAMEAEDLSAKGIDSLPGSLYEAA
VALRDSALMKEVLGEHTHQNLVGNKLIEWDAYRTHVSEFELKRYLPVL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=351, Percent_Identity=27.3504273504274, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1790301, Length=425, Percent_Identity=40.9411764705882, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI87081870, Length=420, Percent_Identity=28.3333333333333, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50644; Mature: 50513

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNYPVFNCKNGDDVIRAVKDYNVSFVQFWFVDILGNLKSFQVTPDELEAAFEEGMGFD
CCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
GSSILGFTRIEESDMIAFPDASSFQICSWRPMDRPVARMFCDIRTPDGNPYEGDPRYVLR
CCCEEEEEEECCCCEEECCCCCCCEEEECCCCCCHHHHHEEEEECCCCCCCCCCHHHHHH
RLVDKAAQKGYTYYVGPELEFFLFADSTCPRPLDSGGYFDAPPLDLGNEVRRDIIFALQK
HHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
MGIDVEYSHHEVAPSQHEIDLRYNEALKMADIAMTYKVVVKEIARKHGVYATFMPKPIFG
HCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
ENGSGMHVHQSLFKNGKNAFFDANDPHHLSAEARHYIAGLLTHASEITCITNQWVNSYKR
CCCCCHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHH
LVPGYEAPVYIAWAQRNRSSLIRVPMYKPGKEAATRIELRNPDPACNPYLAFAAMLGAGL
HCCCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHEEEECCCCCCCCHHHHHHHHHHCCH
EGIEKGYEVPRAVEENIFAMEAEDLSAKGIDSLPGSLYEAAVALRDSALMKEVLGEHTHQ
HHHHHHHCCCHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NLVGNKLIEWDAYRTHVSEFELKRYLPVL
HHHHCCEEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STNYPVFNCKNGDDVIRAVKDYNVSFVQFWFVDILGNLKSFQVTPDELEAAFEEGMGFD
CCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
GSSILGFTRIEESDMIAFPDASSFQICSWRPMDRPVARMFCDIRTPDGNPYEGDPRYVLR
CCCEEEEEEECCCCEEECCCCCCCEEEECCCCCCHHHHHEEEEECCCCCCCCCCHHHHHH
RLVDKAAQKGYTYYVGPELEFFLFADSTCPRPLDSGGYFDAPPLDLGNEVRRDIIFALQK
HHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
MGIDVEYSHHEVAPSQHEIDLRYNEALKMADIAMTYKVVVKEIARKHGVYATFMPKPIFG
HCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
ENGSGMHVHQSLFKNGKNAFFDANDPHHLSAEARHYIAGLLTHASEITCITNQWVNSYKR
CCCCCHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHH
LVPGYEAPVYIAWAQRNRSSLIRVPMYKPGKEAATRIELRNPDPACNPYLAFAAMLGAGL
HCCCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHEEEECCCCCCCCHHHHHHHHHHCCH
EGIEKGYEVPRAVEENIFAMEAEDLSAKGIDSLPGSLYEAAVALRDSALMKEVLGEHTHQ
HHHHHHHCCCHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NLVGNKLIEWDAYRTHVSEFELKRYLPVL
HHHHCCEEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]