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The map label for this gene is nfnB [H]

Identifier: 78355133

GI number: 78355133

Start: 79628

End: 80284

Strand: Direct

Name: nfnB [H]

Synonym: Dde_0086

Alternate gene names: 78355133

Gene position: 79628-80284 (Clockwise)

Preceding gene: 78355131

Following gene: 78355135

Centisome position: 2.13

GC content: 51.75

Gene sequence:

>657_bases
ATGCATAGCTATATTGAAGCTCTTACCACCAGATACACCACAAAAAAGTTTGATCCGGACCGCAGGCTGGACGCCGAAAC
CGTGCAGGCGGTGAAGGATATTCTGCAACTCAGTCCTTCTTCGACCAACTCTCAGCCGTGGCATTTTGTCATTGCCGGTA
CGGATGAAGGCAGGCAGCGCGTGGCAAAGGCGGCACACGGGGTTTACGCATTTAACGCATCAAAAATCTGCGATGCTTCT
CATGTTGTGGTGCTGTGCACAAAAACGGATATCGACACGGAATACACCACACAGGTGCTTGAGCAGGAAGAGCAGGACGG
TCGTTTTCCTTCTGAAGAAACGAAGGCCGCCAACAGAAAAGGCCGTGAGTTTTTTGCCATGCTGCACCGTAACGAGTTGC
AGGACGCTTTTCACTGGATGGAGAAGCAGACGTTTATCGCCCTTGGTAATCTGTTGTCCGGTGCGGCACATCTGGGCATA
CACGCATGCCCCATGGAAGGCTTTGATCACGAAGTGCTGGAAAAAGAGCTGGGATTGACGGAGAAAGGGTACAAGCCCTC
GGTCATTGTGGCTCTGGGGTTTAGTGCCGCTGATGATTTCAATGCGGTGACACCCAAATCCCGCTGGCCTCAGGAACGGA
TAATAACCGAAATATGA

Upstream 100 bases:

>100_bases
TAATTAAAGCGGCTGTAAATAGTAATTTTACTTCAGGCGCATGACCGGCCATTATTACATCGTAACTTCAGGAACCGCAG
ACCATCGCAGGGAGGCAGTC

Downstream 100 bases:

>100_bases
CGTGCGGCAGCAATGCCGCAGTCCCCTGCACCGCGTGCCGGTGCAGGGGATTTTTGTGTGCGGGTAAAGCTGTGCGGCCG
TATGCCCTGCGGGCTATGCC

Product: dihydropteridine reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MHSYIEALTTRYTTKKFDPDRRLDAETVQAVKDILQLSPSSTNSQPWHFVIAGTDEGRQRVAKAAHGVYAFNASKICDAS
HVVVLCTKTDIDTEYTTQVLEQEEQDGRFPSEETKAANRKGREFFAMLHRNELQDAFHWMEKQTFIALGNLLSGAAHLGI
HACPMEGFDHEVLEKELGLTEKGYKPSVIVALGFSAADDFNAVTPKSRWPQERIITEI

Sequences:

>Translated_218_residues
MHSYIEALTTRYTTKKFDPDRRLDAETVQAVKDILQLSPSSTNSQPWHFVIAGTDEGRQRVAKAAHGVYAFNASKICDAS
HVVVLCTKTDIDTEYTTQVLEQEEQDGRFPSEETKAANRKGREFFAMLHRNELQDAFHWMEKQTFIALGNLLSGAAHLGI
HACPMEGFDHEVLEKELGLTEKGYKPSVIVALGFSAADDFNAVTPKSRWPQERIITEI
>Mature_218_residues
MHSYIEALTTRYTTKKFDPDRRLDAETVQAVKDILQLSPSSTNSQPWHFVIAGTDEGRQRVAKAAHGVYAFNASKICDAS
HVVVLCTKTDIDTEYTTQVLEQEEQDGRFPSEETKAANRKGREFFAMLHRNELQDAFHWMEKQTFIALGNLLSGAAHLGI
HACPMEGFDHEVLEKELGLTEKGYKPSVIVALGFSAADDFNAVTPKSRWPQERIITEI

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=214, Percent_Identity=51.4018691588785, Blast_Score=237, Evalue=3e-64,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.-; 1.5.1.34

Molecular weight: Translated: 24513; Mature: 24513

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSYIEALTTRYTTKKFDPDRRLDAETVQAVKDILQLSPSSTNSQPWHFVIAGTDEGRQR
CHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHH
VAKAAHGVYAFNASKICDASHVVVLCTKTDIDTEYTTQVLEQEEQDGRFPSEETKAANRK
HHHHHCCEEEECCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GREFFAMLHRNELQDAFHWMEKQTFIALGNLLSGAAHLGIHACPMEGFDHEVLEKELGLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCC
EKGYKPSVIVALGFSAADDFNAVTPKSRWPQERIITEI
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MHSYIEALTTRYTTKKFDPDRRLDAETVQAVKDILQLSPSSTNSQPWHFVIAGTDEGRQR
CHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHH
VAKAAHGVYAFNASKICDASHVVVLCTKTDIDTEYTTQVLEQEEQDGRFPSEETKAANRK
HHHHHCCEEEECCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GREFFAMLHRNELQDAFHWMEKQTFIALGNLLSGAAHLGIHACPMEGFDHEVLEKELGLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCC
EKGYKPSVIVALGFSAADDFNAVTPKSRWPQERIITEI
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179862; 11677609 [H]