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The map label for this gene is dpnM [H]

Identifier: 78224331

GI number: 78224331

Start: 3535829

End: 3537745

Strand: Reverse

Name: dpnM [H]

Synonym: Gmet_3140

Alternate gene names: 78224331

Gene position: 3537745-3535829 (Counterclockwise)

Preceding gene: 78224333

Following gene: 78224330

Centisome position: 88.5

GC content: 49.45

Gene sequence:

>1917_bases
ATGACAAAATCAAAAACAAACAGAAATATCAGTGAGTTGTGCGATTTGTCTGGTTGCCGACCTCTTTTGAAGTGGGCAGG
CGGCAAGTCGCAACTGCTTGGGCAACTGTTGCCCAAGATGCCGAAGAAGTACGGTCGCTATATCGAGCCATTCTTCGGCG
GCGGAGCATTGTTCTTTGCGGTCAAGCCGGAAGATGCCATTATTGCCGACAGCAACCCGGAACTGGTCAACCTGTACCAA
TCGGTTGCCGACAATGTTGACAATGTTATCCGGTGCCTGAAACGCCACAATAACACGGAAGACGATTTCTATAAGGTCAG
GGCGCAGGACTGGACCAAGCTTTCGCCAGCGTCCGCAGCGGCTCGGACAATCTTCTTGAACCGGACCTGCTTCAATGGAT
TGTACCGGGTCAACAAAAGCGGTCAGTTCAACTCGCCCTATGGTCGGTACGTCAATCCCCGCATTCTTGACGAAGAAGGA
TTGCGTGCCGCTTCTTACCTGTTGAGCAAGGCAACGATAGTTCTTGGCGACTATAAGGACGTACTACGGGAACACGCACG
TTCCGGTGATTTTGTCTTCCTTGACCCGCCATATCTCCCGGTATCGGCGTATGCTGATTTCAAGCGATACACCAAAGAAC
AGTTCTACGAGGAAGACCATGTGGAACTTGCTACGGAAATCGCCCGCCTGCATGAACTAGGGTGCTATGTCATATTGACT
AACAGCAACAATCCCTTGGTGCATGAACTATACGGGCGTTATGAAATGTACGTCGTTGAGACCAAGCGCCATATCAGTTG
TAATGGACAAGGACGCACAGGGCAAGATGTTATCGTTGACATCAAGCCCAAGGCACGTCTGAACCTGAAACTTGTGCCGG
AACCACTTCCGGAACAGGCTTTGCTGTATCCGCCTACCCGTTATATGGGAAGCAAGAACAAACTCCTGAAAGACATTTGG
GCAGTAGCGTCCCAGTTTGACTTCGATACCGCCGTTGACCTTTTTTCCGGTAGCGGGATTGTCGGCTATATGTTCAAGTC
TCATGGCAAGGCGGTCATATCGAATGACTACATGCACATGTCGGCGTGCTTTACAAAAGCAATGGTCGAGAATAATTCCG
TTATTCTTCCGCTTGAAGAAGCACGGAGACTGTTGGAACCAAAGCACAAGTCTGACGGCTTCGTGGCGGAGAAGTTCAAG
GGTCTTTACTTCGAGGATGCCGATAATCGCCTGATTGATGTCTTGAGGTCAAATATTTGGGCATTGAAGAATCAATATAA
GAAGGCAATCGCCCTTTCGGCGCTTATCAGAGCCTGCACCAAGAAGCGTCCACGCGGGATATTCACTTATACCGGTCTTG
GTCGCTATGACGACGGGCGGCGTGATCTTCACCTGTCATTTGCGGACCAGTTCCTAGAAGCTGTGGAAGCGATTAACAGT
GCCGTTTTTGATAATGGGCATGAAAACAAATCACGCCACGGCGACGCTTTGACCGTACCGGTCGTAGAAAATGCACTGGT
CTACATTGACCCGCCATATTACAGCCCGCTGTCGGACAACGAGTATGTCCGCCGGTATCACTTCGTTGAAGGGCTGGCAA
GGAACTGGAAGGGTGTGGAGATACAGGAACACACCCAGACCAAGAAATTCAAATCCTATCCGACACCATTTTCCACAAGA
AAGGGTGCTGCGGACGCCTTTGACCACTTGTTTAAGCGGTTCAAAAACAATATTCTGATAGTATCATACTCGTCCAACAG
CCAGCCCACGCAGGACGAGATGCTTGAAATAATGGGCAAGTACAAGACCAATGTTGCGGTCATTCCGGTGGACTACAAGT
ATTCATTCGGAACACATGGGCACAAGGTCGGAGATAACAATAACGACGTGCAAGAATACTTATTTGTTGGGTATTGA

Upstream 100 bases:

>100_bases
CATTTATAGCAGCGAAAATATACAGCAGGGTCCATGGAATTACTTTGCATTGTGGCTAAATTTTAGAGTAGATTGTACCC
TTTGAACATCGCTATCTATT

Downstream 100 bases:

>100_bases
GAATGATTTCCCAAAGCGACTTTCCGCAAGCAGACAGACTTGAAAAAGTCGGTGAAGTCGCTATTGCTGTATCACAAGGG
AACTTTTCGGATGTCAGCAT

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.DpnIIA; Adenine-specific methyltransferase DpnIIA; M.DpnII 1 [H]

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MTKSKTNRNISELCDLSGCRPLLKWAGGKSQLLGQLLPKMPKKYGRYIEPFFGGGALFFAVKPEDAIIADSNPELVNLYQ
SVADNVDNVIRCLKRHNNTEDDFYKVRAQDWTKLSPASAAARTIFLNRTCFNGLYRVNKSGQFNSPYGRYVNPRILDEEG
LRAASYLLSKATIVLGDYKDVLREHARSGDFVFLDPPYLPVSAYADFKRYTKEQFYEEDHVELATEIARLHELGCYVILT
NSNNPLVHELYGRYEMYVVETKRHISCNGQGRTGQDVIVDIKPKARLNLKLVPEPLPEQALLYPPTRYMGSKNKLLKDIW
AVASQFDFDTAVDLFSGSGIVGYMFKSHGKAVISNDYMHMSACFTKAMVENNSVILPLEEARRLLEPKHKSDGFVAEKFK
GLYFEDADNRLIDVLRSNIWALKNQYKKAIALSALIRACTKKRPRGIFTYTGLGRYDDGRRDLHLSFADQFLEAVEAINS
AVFDNGHENKSRHGDALTVPVVENALVYIDPPYYSPLSDNEYVRRYHFVEGLARNWKGVEIQEHTQTKKFKSYPTPFSTR
KGAADAFDHLFKRFKNNILIVSYSSNSQPTQDEMLEIMGKYKTNVAVIPVDYKYSFGTHGHKVGDNNNDVQEYLFVGY

Sequences:

>Translated_638_residues
MTKSKTNRNISELCDLSGCRPLLKWAGGKSQLLGQLLPKMPKKYGRYIEPFFGGGALFFAVKPEDAIIADSNPELVNLYQ
SVADNVDNVIRCLKRHNNTEDDFYKVRAQDWTKLSPASAAARTIFLNRTCFNGLYRVNKSGQFNSPYGRYVNPRILDEEG
LRAASYLLSKATIVLGDYKDVLREHARSGDFVFLDPPYLPVSAYADFKRYTKEQFYEEDHVELATEIARLHELGCYVILT
NSNNPLVHELYGRYEMYVVETKRHISCNGQGRTGQDVIVDIKPKARLNLKLVPEPLPEQALLYPPTRYMGSKNKLLKDIW
AVASQFDFDTAVDLFSGSGIVGYMFKSHGKAVISNDYMHMSACFTKAMVENNSVILPLEEARRLLEPKHKSDGFVAEKFK
GLYFEDADNRLIDVLRSNIWALKNQYKKAIALSALIRACTKKRPRGIFTYTGLGRYDDGRRDLHLSFADQFLEAVEAINS
AVFDNGHENKSRHGDALTVPVVENALVYIDPPYYSPLSDNEYVRRYHFVEGLARNWKGVEIQEHTQTKKFKSYPTPFSTR
KGAADAFDHLFKRFKNNILIVSYSSNSQPTQDEMLEIMGKYKTNVAVIPVDYKYSFGTHGHKVGDNNNDVQEYLFVGY
>Mature_637_residues
TKSKTNRNISELCDLSGCRPLLKWAGGKSQLLGQLLPKMPKKYGRYIEPFFGGGALFFAVKPEDAIIADSNPELVNLYQS
VADNVDNVIRCLKRHNNTEDDFYKVRAQDWTKLSPASAAARTIFLNRTCFNGLYRVNKSGQFNSPYGRYVNPRILDEEGL
RAASYLLSKATIVLGDYKDVLREHARSGDFVFLDPPYLPVSAYADFKRYTKEQFYEEDHVELATEIARLHELGCYVILTN
SNNPLVHELYGRYEMYVVETKRHISCNGQGRTGQDVIVDIKPKARLNLKLVPEPLPEQALLYPPTRYMGSKNKLLKDIWA
VASQFDFDTAVDLFSGSGIVGYMFKSHGKAVISNDYMHMSACFTKAMVENNSVILPLEEARRLLEPKHKSDGFVAEKFKG
LYFEDADNRLIDVLRSNIWALKNQYKKAIALSALIRACTKKRPRGIFTYTGLGRYDDGRRDLHLSFADQFLEAVEAINSA
VFDNGHENKSRHGDALTVPVVENALVYIDPPYYSPLSDNEYVRRYHFVEGLARNWKGVEIQEHTQTKKFKSYPTPFSTRK
GAADAFDHLFKRFKNNILIVSYSSNSQPTQDEMLEIMGKYKTNVAVIPVDYKYSFGTHGHKVGDNNNDVQEYLFVGY

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the DpnII endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=268, Percent_Identity=32.8358208955224, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 72657; Mature: 72526

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSKTNRNISELCDLSGCRPLLKWAGGKSQLLGQLLPKMPKKYGRYIEPFFGGGALFFA
CCCCCCCCCHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEE
VKPEDAIIADSNPELVNLYQSVADNVDNVIRCLKRHNNTEDDFYKVRAQDWTKLSPASAA
ECCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCHHHH
ARTIFLNRTCFNGLYRVNKSGQFNSPYGRYVNPRILDEEGLRAASYLLSKATIVLGDYKD
HHEEEEECHHHHHHEEECCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHEEEECCHHH
VLREHARSGDFVFLDPPYLPVSAYADFKRYTKEQFYEEDHVELATEIARLHELGCYVILT
HHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEE
NSNNPLVHELYGRYEMYVVETKRHISCNGQGRTGQDVIVDIKPKARLNLKLVPEPLPEQA
CCCCCHHHHHCCCEEEEEEECCCEEEECCCCCCCCEEEEEECCCCEEEEEECCCCCCCCC
LLYPPTRYMGSKNKLLKDIWAVASQFDFDTAVDLFSGSGIVGYMFKSHGKAVISNDYMHM
EECCCHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCEEEECCCHHH
SACFTKAMVENNSVILPLEEARRLLEPKHKSDGFVAEKFKGLYFEDADNRLIDVLRSNIW
HHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCHHHHHCCCEEECCCHHHHHHHHHHHH
ALKNQYKKAIALSALIRACTKKRPRGIFTYTGLGRYDDGRRDLHLSFADQFLEAVEAINS
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
AVFDNGHENKSRHGDALTVPVVENALVYIDPPYYSPLSDNEYVRRYHFVEGLARNWKGVE
HHHCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
IQEHTQTKKFKSYPTPFSTRKGAADAFDHLFKRFKNNILIVSYSSNSQPTQDEMLEIMGK
EHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHH
YKTNVAVIPVDYKYSFGTHGHKVGDNNNDVQEYLFVGY
HCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHEEECC
>Mature Secondary Structure 
TKSKTNRNISELCDLSGCRPLLKWAGGKSQLLGQLLPKMPKKYGRYIEPFFGGGALFFA
CCCCCCCCHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEE
VKPEDAIIADSNPELVNLYQSVADNVDNVIRCLKRHNNTEDDFYKVRAQDWTKLSPASAA
ECCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCHHHH
ARTIFLNRTCFNGLYRVNKSGQFNSPYGRYVNPRILDEEGLRAASYLLSKATIVLGDYKD
HHEEEEECHHHHHHEEECCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHEEEECCHHH
VLREHARSGDFVFLDPPYLPVSAYADFKRYTKEQFYEEDHVELATEIARLHELGCYVILT
HHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEE
NSNNPLVHELYGRYEMYVVETKRHISCNGQGRTGQDVIVDIKPKARLNLKLVPEPLPEQA
CCCCCHHHHHCCCEEEEEEECCCEEEECCCCCCCCEEEEEECCCCEEEEEECCCCCCCCC
LLYPPTRYMGSKNKLLKDIWAVASQFDFDTAVDLFSGSGIVGYMFKSHGKAVISNDYMHM
EECCCHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCEEEECCCHHH
SACFTKAMVENNSVILPLEEARRLLEPKHKSDGFVAEKFKGLYFEDADNRLIDVLRSNIW
HHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCHHHHHCCCEEECCCHHHHHHHHHHHH
ALKNQYKKAIALSALIRACTKKRPRGIFTYTGLGRYDDGRRDLHLSFADQFLEAVEAINS
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
AVFDNGHENKSRHGDALTVPVVENALVYIDPPYYSPLSDNEYVRRYHFVEGLARNWKGVE
HHHCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
IQEHTQTKKFKSYPTPFSTRKGAADAFDHLFKRFKNNILIVSYSSNSQPTQDEMLEIMGK
EHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHH
YKTNVAVIPVDYKYSFGTHGHKVGDNNNDVQEYLFVGY
HCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989823; 3019562; 2824782; 9862809 [H]