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The map label for this gene is cls2 [H]

Identifier: 78224270

GI number: 78224270

Start: 3475062

End: 3476252

Strand: Reverse

Name: cls2 [H]

Synonym: Gmet_3078

Alternate gene names: 78224270

Gene position: 3476252-3475062 (Counterclockwise)

Preceding gene: 78224271

Following gene: 78224269

Centisome position: 86.96

GC content: 64.06

Gene sequence:

>1191_bases
ATGTCCATCGTGAGACGAAAAAAGATAATCGGTACCCACCGTGCCCCCCGTTTCCTCCGCATCTTCCGGAGAAATGCCGA
GGCGTCCCATTCCCGGGGGAACAGGGTGAAGCTCTACGATTCCGGTGCGGAGTTCTTCCCGGCCATGCTGTCGGCTCTGG
CCGAGGCCCGTCACCACATCCACGCCGAGTTCTACATCGTGCGCGACGACGCCACCGGATCCGCCTTTGCGGAGGCGCTT
CTGGCCGCCGCTTCCCGCAGGGTCGACGTTTCCCTCATCTACGATTACATCGGCTGTTTCGATACTCCCTCCTCCTATTT
CCGGCGCCTGGAGCAGGGGGGAGTCCGGTGCCTCGCCTTCAATCCTCCCCCATTCCGGCGGGGACTCGCCTGGTTCGACA
AGCGGGACCACCGGAAGATGGCGGTCATCGACGGAGAAACCGCCTTCACCGGCGGAATCAACATCGGCGACGAGTACTCG
GGGTTCGGGAGGGGAACCGACCGCTGGCGGGACGTGGGGGTGAGAATCGACGGGCCCGCCGTCACGGACCTCGTGCGGCT
CTTCCGCGAGTTCTGGCTGGAGGAAAAGGGGAGCCCTTCTCCGGCCTGGTTTGCGGAGCATATTCCCGTGGCAGGCATGG
GAGATGCCGACGTGATGATCGTGAGCGACGGCCCCCACCACGCCCGTTCCTTCATTCGCAACGCCTTCCGCATCGCCATG
GCCGGAGCGGGAGAATCGATCCGGATCATGAACCCCTACTTCGTGCCGGGTCCGCGGGTGGTCCGCTCTCTGCTGCGGGC
CGTGCGGCGTGGGGTAAACGTTCAATTGATCCTCCCCGCCAAGAGTGACGTCCCCATCGTCCGGCTCGTGGGGCGCGGTT
ACTACGCCCCCCTCCTTCGGGCCGGCGTCGAGATCTACGAGCGCCAGGGGGAGTTTCTCCATGCCAAGGTCATGCTCATC
GACGACACCTGGGGGGTGGTCGGCTCGGCAAATCTCGACCAGCGCAGCTTCCATCGCAACTACGAAGTCAACACCATTGT
TGCCAGCCGCGAGTTCGGCCGGCAGATGGCGGAGATGTTCGCCGACGACCTCAGGGGGTCCCGCCGTATCGTTCTAGAGG
AGCATGAGCGCCGGGGCTCGCTGGTGCGGCTTCTGGAGCGGATTTGCAATTCGGTGAGCTGGTTTCTGTGA

Upstream 100 bases:

>100_bases
GAGGCGCGGGGGAGACTTCGCGCCTCTTCGCTTTTCCCCTCCATCATCTCCCTTGCCCTCCTTGCCCGCCTGCGTTACCC
TTGATGCAGGAGTCCCGGGC

Downstream 100 bases:

>100_bases
TTCGGTTTTGTGTTTGAAAAGTGACCACTTTTTGTGTAGAGTTGTAACTACTTAAAGTATGTCTCGTTCCGGTGAGGTGA
TGCCATGATAGCGGTAGGGA

Product: phospholipase D/transphosphatidylase

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSIVRRKKIIGTHRAPRFLRIFRRNAEASHSRGNRVKLYDSGAEFFPAMLSALAEARHHIHAEFYIVRDDATGSAFAEAL
LAAASRRVDVSLIYDYIGCFDTPSSYFRRLEQGGVRCLAFNPPPFRRGLAWFDKRDHRKMAVIDGETAFTGGINIGDEYS
GFGRGTDRWRDVGVRIDGPAVTDLVRLFREFWLEEKGSPSPAWFAEHIPVAGMGDADVMIVSDGPHHARSFIRNAFRIAM
AGAGESIRIMNPYFVPGPRVVRSLLRAVRRGVNVQLILPAKSDVPIVRLVGRGYYAPLLRAGVEIYERQGEFLHAKVMLI
DDTWGVVGSANLDQRSFHRNYEVNTIVASREFGRQMAEMFADDLRGSRRIVLEEHERRGSLVRLLERICNSVSWFL

Sequences:

>Translated_396_residues
MSIVRRKKIIGTHRAPRFLRIFRRNAEASHSRGNRVKLYDSGAEFFPAMLSALAEARHHIHAEFYIVRDDATGSAFAEAL
LAAASRRVDVSLIYDYIGCFDTPSSYFRRLEQGGVRCLAFNPPPFRRGLAWFDKRDHRKMAVIDGETAFTGGINIGDEYS
GFGRGTDRWRDVGVRIDGPAVTDLVRLFREFWLEEKGSPSPAWFAEHIPVAGMGDADVMIVSDGPHHARSFIRNAFRIAM
AGAGESIRIMNPYFVPGPRVVRSLLRAVRRGVNVQLILPAKSDVPIVRLVGRGYYAPLLRAGVEIYERQGEFLHAKVMLI
DDTWGVVGSANLDQRSFHRNYEVNTIVASREFGRQMAEMFADDLRGSRRIVLEEHERRGSLVRLLERICNSVSWFL
>Mature_395_residues
SIVRRKKIIGTHRAPRFLRIFRRNAEASHSRGNRVKLYDSGAEFFPAMLSALAEARHHIHAEFYIVRDDATGSAFAEALL
AAASRRVDVSLIYDYIGCFDTPSSYFRRLEQGGVRCLAFNPPPFRRGLAWFDKRDHRKMAVIDGETAFTGGINIGDEYSG
FGRGTDRWRDVGVRIDGPAVTDLVRLFREFWLEEKGSPSPAWFAEHIPVAGMGDADVMIVSDGPHHARSFIRNAFRIAMA
GAGESIRIMNPYFVPGPRVVRSLLRAVRRGVNVQLILPAKSDVPIVRLVGRGYYAPLLRAGVEIYERQGEFLHAKVMLID
DTWGVVGSANLDQRSFHRNYEVNTIVASREFGRQMAEMFADDLRGSRRIVLEEHERRGSLVRLLERICNSVSWFL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=346, Percent_Identity=33.8150289017341, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787502, Length=383, Percent_Identity=30.5483028720627, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87081826, Length=385, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 44810; Mature: 44679

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVRRKKIIGTHRAPRFLRIFRRNAEASHSRGNRVKLYDSGAEFFPAMLSALAEARHHI
CCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHE
HAEFYIVRDDATGSAFAEALLAAASRRVDVSLIYDYIGCFDTPSSYFRRLEQGGVRCLAF
EEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEE
NPPPFRRGLAWFDKRDHRKMAVIDGETAFTGGINIGDEYSGFGRGTDRWRDVGVRIDGPA
CCCCHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCHHHHCCEEECCHH
VTDLVRLFREFWLEEKGSPSPAWFAEHIPVAGMGDADVMIVSDGPHHARSFIRNAFRIAM
HHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHEEE
AGAGESIRIMNPYFVPGPRVVRSLLRAVRRGVNVQLILPAKSDVPIVRLVGRGYYAPLLR
ECCCCEEEEECCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCHHHHHHH
AGVEIYERQGEFLHAKVMLIDDTWGVVGSANLDQRSFHRNYEVNTIVASREFGRQMAEMF
HHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCHHHHHCCCCEEEEEECHHHHHHHHHHH
ADDLRGSRRIVLEEHERRGSLVRLLERICNSVSWFL
HHHCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIVRRKKIIGTHRAPRFLRIFRRNAEASHSRGNRVKLYDSGAEFFPAMLSALAEARHHI
CCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHE
HAEFYIVRDDATGSAFAEALLAAASRRVDVSLIYDYIGCFDTPSSYFRRLEQGGVRCLAF
EEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEE
NPPPFRRGLAWFDKRDHRKMAVIDGETAFTGGINIGDEYSGFGRGTDRWRDVGVRIDGPA
CCCCHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCHHHHCCEEECCHH
VTDLVRLFREFWLEEKGSPSPAWFAEHIPVAGMGDADVMIVSDGPHHARSFIRNAFRIAM
HHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHEEE
AGAGESIRIMNPYFVPGPRVVRSLLRAVRRGVNVQLILPAKSDVPIVRLVGRGYYAPLLR
ECCCCEEEEECCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCHHHHHHH
AGVEIYERQGEFLHAKVMLIDDTWGVVGSANLDQRSFHRNYEVNTIVASREFGRQMAEMF
HHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCHHHHHCCCCEEEEEECHHHHHHHHHHH
ADDLRGSRRIVLEEHERRGSLVRLLERICNSVSWFL
HHHCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]