Click here to switch to the map view.

The map label for this gene is aprN [H]

Identifier: 78222661

GI number: 78222661

Start: 1619388

End: 1620728

Strand: Reverse

Name: aprN [H]

Synonym: Gmet_1449

Alternate gene names: 78222661

Gene position: 1620728-1619388 (Counterclockwise)

Preceding gene: 78222662

Following gene: 78222641

Centisome position: 40.54

GC content: 63.83

Gene sequence:

>1341_bases
ATGCCCGCACATCGTCTTCCCGTCATCCGGAAAATCCTCGCCGCCACATTCCTTGCCGCCCTTTTCAGTCAAAGCCCCGC
GTCAGTCGCTGCCGCTGCGCCCGTTGAACCACCGGGCATGCGGCAACGGCTTTCGTCCGGCGCTCCCCAGGAGATGCTGA
TCCTGCTCGATGAGGGTGCCATTGCCAAATCCCACGTTATCGGCGCACTCAGGGCCGAGGGGGGAGAGGTGGTAACAGAT
TACTCCCACCTGCCGATGCTCTTCGCCAGAATTCGATCCACAAAGGCCCTTGACCGTCTCCTGACGGCTCCCGGCATCCA
GGCTGTGTACGAAAACCGCGTCCTCCGGCCCCATCTCGCCGAGAGCCTCCCCCTCATCGGCCAGCCCCCACTCGCCGCCA
TCGGGAAAACCGGCAGCGGCACAACCGTGGCGATAATCGACAGCGGGATAAACTATACGCTGCCGGCCTTCGGCTCCTGC
ACCGCGCCGGGGGTTCCGGCCGACTGCAAGGTGGCGGCATCGGTGGATATTGCCACCAACGACAACGCCCTGGACGACCT
GGGCCACGGCACCAACGTGGCCGGTATTGTCGCCGGTGTCGCTCCCGGTGCGAAACTAGCCGTTCTCGACGTGTTCAATC
CCGACGGCACCTCTTCCGATGCCCTGGTTATCGCAGGAATCAACTGGGCCATTTCAAACCGGGCAACGTACAATATTGTG
GCCATCAACATGAGCCTGGGGGATGGTAGCCGCAACACCGCAGCATGCAGCAACAGGGGGACCAATCCCTACGTGACCCC
CATCAATAACGCCAAGAGCGCGGGAATCGTGACAGTCATCTCGGCAGGGAACGAAGGGTATACAGACGGCATCTCACGCC
CCGCCTGCACCCCGGCCGCGGTGTCGGTGGGTGCGGTGTACGACGCGAACGTGGGAGGGATCAAGTGGACCGCCTGCACC
GACGCGACCAGCGGTGCCGACAAGGTCCCCTGCATGTCGAACACCGCCACGTTCCTCAACATGCTGGCGCCGGGGGCATT
CATCACTGCCGCCGGCTCGACCATGGGCGGGACGTCCCAGGCGGCGCCCCACGTGGCGGGTTCCGTGGCGGTACTGAAGG
CCCAATACCCCAATGAAACGCCGGACCAGCTCATCTCCCGTCTCATTTCCTCGGGAAAACCGGTGACCGACCAGCGCACC
GGCGGGGTCTTCCCGCGGCTCGATCTTTTTTCGTCAATGGGGTTGCCGGACTCGAATTTCGTGCCGGCGATGTCACCTTG
GGGTATCGGCCTCTCGGCGGTCTTTCTCGGCGGAGCCCTGGCGGTGCGCAGACGGTCTTGA

Upstream 100 bases:

>100_bases
CACGCACATTCACACACTTTCGGCCCCGTCGTAACACCGAAGAGAACTTCATCTGCCATTCTTAAGCATGAATCAATCCT
CCCGCCAAATGGAGACTGTC

Downstream 100 bases:

>100_bases
TACCCCATGGCACAGGATGAACCACAGCAGAAACGGCAGGAGATATCCGAGGATTTCATGAATGGCCACGGAGGCAAGGT
AGGCCTCCCGGATGCCGAAA

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MPAHRLPVIRKILAATFLAALFSQSPASVAAAAPVEPPGMRQRLSSGAPQEMLILLDEGAIAKSHVIGALRAEGGEVVTD
YSHLPMLFARIRSTKALDRLLTAPGIQAVYENRVLRPHLAESLPLIGQPPLAAIGKTGSGTTVAIIDSGINYTLPAFGSC
TAPGVPADCKVAASVDIATNDNALDDLGHGTNVAGIVAGVAPGAKLAVLDVFNPDGTSSDALVIAGINWAISNRATYNIV
AINMSLGDGSRNTAACSNRGTNPYVTPINNAKSAGIVTVISAGNEGYTDGISRPACTPAAVSVGAVYDANVGGIKWTACT
DATSGADKVPCMSNTATFLNMLAPGAFITAAGSTMGGTSQAAPHVAGSVAVLKAQYPNETPDQLISRLISSGKPVTDQRT
GGVFPRLDLFSSMGLPDSNFVPAMSPWGIGLSAVFLGGALAVRRRS

Sequences:

>Translated_446_residues
MPAHRLPVIRKILAATFLAALFSQSPASVAAAAPVEPPGMRQRLSSGAPQEMLILLDEGAIAKSHVIGALRAEGGEVVTD
YSHLPMLFARIRSTKALDRLLTAPGIQAVYENRVLRPHLAESLPLIGQPPLAAIGKTGSGTTVAIIDSGINYTLPAFGSC
TAPGVPADCKVAASVDIATNDNALDDLGHGTNVAGIVAGVAPGAKLAVLDVFNPDGTSSDALVIAGINWAISNRATYNIV
AINMSLGDGSRNTAACSNRGTNPYVTPINNAKSAGIVTVISAGNEGYTDGISRPACTPAAVSVGAVYDANVGGIKWTACT
DATSGADKVPCMSNTATFLNMLAPGAFITAAGSTMGGTSQAAPHVAGSVAVLKAQYPNETPDQLISRLISSGKPVTDQRT
GGVFPRLDLFSSMGLPDSNFVPAMSPWGIGLSAVFLGGALAVRRRS
>Mature_445_residues
PAHRLPVIRKILAATFLAALFSQSPASVAAAAPVEPPGMRQRLSSGAPQEMLILLDEGAIAKSHVIGALRAEGGEVVTDY
SHLPMLFARIRSTKALDRLLTAPGIQAVYENRVLRPHLAESLPLIGQPPLAAIGKTGSGTTVAIIDSGINYTLPAFGSCT
APGVPADCKVAASVDIATNDNALDDLGHGTNVAGIVAGVAPGAKLAVLDVFNPDGTSSDALVIAGINWAISNRATYNIVA
INMSLGDGSRNTAACSNRGTNPYVTPINNAKSAGIVTVISAGNEGYTDGISRPACTPAAVSVGAVYDANVGGIKWTACTD
ATSGADKVPCMSNTATFLNMLAPGAFITAAGSTMGGTSQAAPHVAGSVAVLKAQYPNETPDQLISRLISSGKPVTDQRTG
GVFPRLDLFSSMGLPDSNFVPAMSPWGIGLSAVFLGGALAVRRRS

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320775, Length=279, Percent_Identity=27.5985663082437, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324576, Length=253, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 45434; Mature: 45303

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHRLPVIRKILAATFLAALFSQSPASVAAAAPVEPPGMRQRLSSGAPQEMLILLDEGA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHCCCCCEEEEEECCCC
IAKSHVIGALRAEGGEVVTDYSHLPMLFARIRSTKALDRLLTAPGIQAVYENRVLRPHLA
CHHHHHHEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHH
ESLPLIGQPPLAAIGKTGSGTTVAIIDSGINYTLPAFGSCTAPGVPADCKVAASVDIATN
HCCCCCCCCCHHHHCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEEEEEEEEECC
DNALDDLGHGTNVAGIVAGVAPGAKLAVLDVFNPDGTSSDALVIAGINWAISNRATYNIV
CCHHHHCCCCCCHHHHEEECCCCCEEEEEEEECCCCCCCCEEEEEECCEEECCCCEEEEE
AINMSLGDGSRNTAACSNRGTNPYVTPINNAKSAGIVTVISAGNEGYTDGISRPACTPAA
EEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHH
VSVGAVYDANVGGIKWTACTDATSGADKVPCMSNTATFLNMLAPGAFITAAGSTMGGTSQ
EEECEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCC
AAPHVAGSVAVLKAQYPNETPDQLISRLISSGKPVTDQRTGGVFPRLDLFSSMGLPDSNF
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
VPAMSPWGIGLSAVFLGGALAVRRRS
CCCCCCCCCCHHHHHHCCHHEEEECC
>Mature Secondary Structure 
PAHRLPVIRKILAATFLAALFSQSPASVAAAAPVEPPGMRQRLSSGAPQEMLILLDEGA
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHCCCCCEEEEEECCCC
IAKSHVIGALRAEGGEVVTDYSHLPMLFARIRSTKALDRLLTAPGIQAVYENRVLRPHLA
CHHHHHHEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHH
ESLPLIGQPPLAAIGKTGSGTTVAIIDSGINYTLPAFGSCTAPGVPADCKVAASVDIATN
HCCCCCCCCCHHHHCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEEEEEEEEECC
DNALDDLGHGTNVAGIVAGVAPGAKLAVLDVFNPDGTSSDALVIAGINWAISNRATYNIV
CCHHHHCCCCCCHHHHEEECCCCCEEEEEEEECCCCCCCCEEEEEECCEEECCCCEEEEE
AINMSLGDGSRNTAACSNRGTNPYVTPINNAKSAGIVTVISAGNEGYTDGISRPACTPAA
EEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHH
VSVGAVYDANVGGIKWTACTDATSGADKVPCMSNTATFLNMLAPGAFITAAGSTMGGTSQ
EEECEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCC
AAPHVAGSVAVLKAQYPNETPDQLISRLISSGKPVTDQRTGGVFPRLDLFSSMGLPDSNF
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
VPAMSPWGIGLSAVFLGGALAVRRRS
CCCCCCCCCCHHHHHHCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]