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The map label for this gene is pheT

Identifier: 78222628

GI number: 78222628

Start: 1583776

End: 1586181

Strand: Direct

Name: pheT

Synonym: Gmet_1416

Alternate gene names: 78222628

Gene position: 1583776-1586181 (Clockwise)

Preceding gene: 78222627

Following gene: 78222629

Centisome position: 39.62

GC content: 55.32

Gene sequence:

>2406_bases
ATGATCGTTACGTACAACTGGCTCAAGGAATTCGTTGATTTCGACCTTTCGCCGCAGGAGCTTTCGGATCTTCTGACCAT
GCTTGGTCTCGAAGTGGAAGGAGTTCGCCATGTTGGGGGCGGACTTGATGAGGTCGTCGTTGCCGTTGTGGAGGAGAGGT
CCCAGCATCCCAACGCGGATAAACTTTCCCTCTGCAGGGTGAACAACGGTAAAGAGACGCTTGCCATCGTGTGTGGCGCC
CAGAACTTCAAGGCCGGAGATAAAGTTGCCCTGGCCCAGATAGGTGCAGTTCTGCCGGGCGATTTCAAAATCAAGCGTTC
CAAGATCAGGGGCGAGGAGTCCTGCGGGATGCTTTGCTCCGAGAAGGAACTTGGTCTGGCCGCTGAGTCCGAGGGGATCA
TCATTCTTCCGACAGACCTTCCCCTTGGCGTTCCGGTATTTGATGCCCTTGGCCTCAAAGATACCATATTCGAGATTGGA
CTAACGCCTAACAGAGCTGATTGTCTGAGCGTTGTGGGTATTGCTCGTGAGATTGCCGCGAAGCTCGGCACCACCGTCAA
GTATGCCCGTCCCTCGGTGAAGGAATCTGTCGTCCCTATCAGTGAGCGCGCTCAGGTGATTGTGGAAGACTCCGATCTGT
GCCCGCGCTATACGGCCCGTTACATTGCCGGCTGCTCCATCGGGCCGTCACCGGCGTGGCTTGTAAGGCGCCTTGAGGCA
GTGGGAATGCGTTCCATCAATAATGTGGTGGATGTCACCAACTATGTCCTCATGGAGTACGGCCATCCCCTCCACGCGTT
CGATGCCGATCTCCTTGCCGGCGGTAAGATTGTTGTGCGTCGTGCCGCAGCAGGGGAGCGCTTCACCACCCTTGACGGCC
AGGAGCGTGTTCTGACGGAGTCCGACCTGACGATTCGGGACGGCGAGAAAGGGGTTGCCCTGGCCGGGATCATGGGGGGA
GAGAACTCGGAGATTCGTCAGGAGACCTCCAACATCCTTCTGGAGAGCGCTTATTTCAATCCGTCTGCGATCCGTAGAAC
CTCGAAGCGTCTCGGTCTCCACACAGAGTCATCCCACCGTTTCGAGCGCGGTGCTGATGTCGACATCCTTGTTACGGCCC
TTGACCGTGCCGCCTCGTTGATTGCTGAGCTTGCGGGGGGGAGAGTTGCCACGGGGACCATTGATGTCTATCCCCGTCCT
TTGCCCCGCCGCACCGTCAGGTTTCGCGTCGACCAGTGCAACAGGTTGCTGGGCATTCAGTTAACTGCCGATGAGATGGC
AGCTATCTTTAATCGCCTTGAGTTTAAAGTTAACGTGGTTGAACCGGAGCTCTTTGACATCGACGTTCCCTCCTGCCGGG
TCGATATCGAGCGGGAGATCGATCTTGTTGAGGAGGTGGCACGGCTTAACGGCTATAACAACATTCCGGTCACCATGCCC
AAGGCCCGGGTCTTTTCCGACCGTCCCACACGCCACCAGCGCCTCGAAAAACAGCTGAGGAATGTCATGGTGGGGCAAGG
CTTCAGCGAGGTGATCACCTTTAGCTTCATGGCTCCCGGGATACTTGACAAGCTTCTCTTGGCCCATGACGACCCCAGGC
GCTCCGTTGTGCGGTTGCGCAACCCCCTTGTGGAAGAGCAGTCGGTGATGCGCACGACGCTTCTTCCCGGACTTCTCGAT
GTAGCTGCAAGAAACATCAATTATCGCATGCTCACGCTTCGGCTCTTTGAGTTGCGGCGCGTCTATCTGCCGGCCGAGGG
GCAGGAGTTGCCCCGGGAGCCCCTCCACCTTGCAGGAATCATGACCGGCGTTCGCTATCGTGAGGGGTGGAATCAGGAGC
GGCATCAGGTTGATTTCTATGACGTAAAGGGTGTTATCGAACATATCCTTGACGAGTTTGCCATCGGATCTGTTGTGTTT
GTCCAGGATCGTCTCGATCCTTATTTTCACCCGGGCAAGGCATGCTCGATCCTCTGTGGCAACGAACTGCTCGGCTCGTT
TGGTGAGCTCCATCCCGATATTCATGACAATTTCGGCATTGATCAGTCCGTCTACTATCTTGACCTCGATTTTGAGAAGC
TCGCTATGGTGAGTTCGGACAACGTTGCCATTAAGCCGCCCTCCCGGTTCCCTGATACGTTCCGCGACATTGCCATGCTT
ATCAAAGATGAAACTCCGGCATCGACGATCGTGGAGTGCATCAGTGGACTGAGAATACGTGAGGTGGAATGCGCCGAGAT
ATTTGACCACTATAAGGGATCTCATGTTCCTGAGGGATTCAAGAGCATTGCGGTTAGAATCAGGTACCGTTCCCATGAAA
GAACACTTGTCGACAACGAGGTTACACCGCTGCATCAACGTATTGTCGATACTCTGGTTAAAAAGTTAGCGGTCACGATA
CGTTAA

Upstream 100 bases:

>100_bases
AACGATGTCCGTTTCCTGCAGCAATTCTGAAATTACAGTGCATCGGGATTATTCGTTCCAGGTAGAGCAATACTGATTAT
GTCCCGGATAGGGTGAGAAT

Downstream 100 bases:

>100_bases
AAACAGGTTGATTATCTGGAACTGCTGTGTTATAAATCCTGTCGCCTTCGGTGGACGCGATATATTTGATAACGTATTGA
TTTTGAGGGAGTATATGACC

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MIVTYNWLKEFVDFDLSPQELSDLLTMLGLEVEGVRHVGGGLDEVVVAVVEERSQHPNADKLSLCRVNNGKETLAIVCGA
QNFKAGDKVALAQIGAVLPGDFKIKRSKIRGEESCGMLCSEKELGLAAESEGIIILPTDLPLGVPVFDALGLKDTIFEIG
LTPNRADCLSVVGIAREIAAKLGTTVKYARPSVKESVVPISERAQVIVEDSDLCPRYTARYIAGCSIGPSPAWLVRRLEA
VGMRSINNVVDVTNYVLMEYGHPLHAFDADLLAGGKIVVRRAAAGERFTTLDGQERVLTESDLTIRDGEKGVALAGIMGG
ENSEIRQETSNILLESAYFNPSAIRRTSKRLGLHTESSHRFERGADVDILVTALDRAASLIAELAGGRVATGTIDVYPRP
LPRRTVRFRVDQCNRLLGIQLTADEMAAIFNRLEFKVNVVEPELFDIDVPSCRVDIEREIDLVEEVARLNGYNNIPVTMP
KARVFSDRPTRHQRLEKQLRNVMVGQGFSEVITFSFMAPGILDKLLLAHDDPRRSVVRLRNPLVEEQSVMRTTLLPGLLD
VAARNINYRMLTLRLFELRRVYLPAEGQELPREPLHLAGIMTGVRYREGWNQERHQVDFYDVKGVIEHILDEFAIGSVVF
VQDRLDPYFHPGKACSILCGNELLGSFGELHPDIHDNFGIDQSVYYLDLDFEKLAMVSSDNVAIKPPSRFPDTFRDIAML
IKDETPASTIVECISGLRIREVECAEIFDHYKGSHVPEGFKSIAVRIRYRSHERTLVDNEVTPLHQRIVDTLVKKLAVTI
R

Sequences:

>Translated_801_residues
MIVTYNWLKEFVDFDLSPQELSDLLTMLGLEVEGVRHVGGGLDEVVVAVVEERSQHPNADKLSLCRVNNGKETLAIVCGA
QNFKAGDKVALAQIGAVLPGDFKIKRSKIRGEESCGMLCSEKELGLAAESEGIIILPTDLPLGVPVFDALGLKDTIFEIG
LTPNRADCLSVVGIAREIAAKLGTTVKYARPSVKESVVPISERAQVIVEDSDLCPRYTARYIAGCSIGPSPAWLVRRLEA
VGMRSINNVVDVTNYVLMEYGHPLHAFDADLLAGGKIVVRRAAAGERFTTLDGQERVLTESDLTIRDGEKGVALAGIMGG
ENSEIRQETSNILLESAYFNPSAIRRTSKRLGLHTESSHRFERGADVDILVTALDRAASLIAELAGGRVATGTIDVYPRP
LPRRTVRFRVDQCNRLLGIQLTADEMAAIFNRLEFKVNVVEPELFDIDVPSCRVDIEREIDLVEEVARLNGYNNIPVTMP
KARVFSDRPTRHQRLEKQLRNVMVGQGFSEVITFSFMAPGILDKLLLAHDDPRRSVVRLRNPLVEEQSVMRTTLLPGLLD
VAARNINYRMLTLRLFELRRVYLPAEGQELPREPLHLAGIMTGVRYREGWNQERHQVDFYDVKGVIEHILDEFAIGSVVF
VQDRLDPYFHPGKACSILCGNELLGSFGELHPDIHDNFGIDQSVYYLDLDFEKLAMVSSDNVAIKPPSRFPDTFRDIAML
IKDETPASTIVECISGLRIREVECAEIFDHYKGSHVPEGFKSIAVRIRYRSHERTLVDNEVTPLHQRIVDTLVKKLAVTI
R
>Mature_801_residues
MIVTYNWLKEFVDFDLSPQELSDLLTMLGLEVEGVRHVGGGLDEVVVAVVEERSQHPNADKLSLCRVNNGKETLAIVCGA
QNFKAGDKVALAQIGAVLPGDFKIKRSKIRGEESCGMLCSEKELGLAAESEGIIILPTDLPLGVPVFDALGLKDTIFEIG
LTPNRADCLSVVGIAREIAAKLGTTVKYARPSVKESVVPISERAQVIVEDSDLCPRYTARYIAGCSIGPSPAWLVRRLEA
VGMRSINNVVDVTNYVLMEYGHPLHAFDADLLAGGKIVVRRAAAGERFTTLDGQERVLTESDLTIRDGEKGVALAGIMGG
ENSEIRQETSNILLESAYFNPSAIRRTSKRLGLHTESSHRFERGADVDILVTALDRAASLIAELAGGRVATGTIDVYPRP
LPRRTVRFRVDQCNRLLGIQLTADEMAAIFNRLEFKVNVVEPELFDIDVPSCRVDIEREIDLVEEVARLNGYNNIPVTMP
KARVFSDRPTRHQRLEKQLRNVMVGQGFSEVITFSFMAPGILDKLLLAHDDPRRSVVRLRNPLVEEQSVMRTTLLPGLLD
VAARNINYRMLTLRLFELRRVYLPAEGQELPREPLHLAGIMTGVRYREGWNQERHQVDFYDVKGVIEHILDEFAIGSVVF
VQDRLDPYFHPGKACSILCGNELLGSFGELHPDIHDNFGIDQSVYYLDLDFEKLAMVSSDNVAIKPPSRFPDTFRDIAML
IKDETPASTIVECISGLRIREVECAEIFDHYKGSHVPEGFKSIAVRIRYRSHERTLVDNEVTPLHQRIVDTLVKKLAVTI
R

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=391, Percent_Identity=26.3427109974425, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1788006, Length=806, Percent_Identity=39.0818858560794, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17533403, Length=380, Percent_Identity=25, Blast_Score=95, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323089, Length=406, Percent_Identity=22.6600985221675, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355211, Length=382, Percent_Identity=25.130890052356, Blast_Score=94, Evalue=5e-19,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_GEOMG (Q39VS4)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384375.1
- ProteinModelPortal:   Q39VS4
- SMR:   Q39VS4
- STRING:   Q39VS4
- GeneID:   3738943
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1416
- NMPDR:   fig|269799.3.peg.1257
- eggNOG:   COG0072
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- PhylomeDB:   Q39VS4
- ProtClustDB:   PRK00629
- BioCyc:   GMET269799:GMET_1416-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 89220; Mature: 89220

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTYNWLKEFVDFDLSPQELSDLLTMLGLEVEGVRHVGGGLDEVVVAVVEERSQHPNAD
CEEEHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCC
KLSLCRVNNGKETLAIVCGAQNFKAGDKVALAQIGAVLPGDFKIKRSKIRGEESCGMLCS
CEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCEEEHHHCCCCHHCCCEEC
EKELGLAAESEGIIILPTDLPLGVPVFDALGLKDTIFEIGLTPNRADCLSVVGIAREIAA
CCCCCCEECCCCEEEEECCCCCCCCHHHHCCCHHHHHEECCCCCHHHHHHHHHHHHHHHH
KLGTTVKYARPSVKESVVPISERAQVIVEDSDLCPRYTARYIAGCSIGPSPAWLVRRLEA
HHCCCEECCCCCHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
VGMRSINNVVDVTNYVLMEYGHPLHAFDADLLAGGKIVVRRAAAGERFTTLDGQERVLTE
HHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHCCCCEEEEECCCCCCEEECCCCCCEEEC
SDLTIRDGEKGVALAGIMGGENSEIRQETSNILLESAYFNPSAIRRTSKRLGLHTESSHR
CCCEEECCCCCEEEEEEECCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCCCCC
FERGADVDILVTALDRAASLIAELAGGRVATGTIDVYPRPLPRRTVRFRVDQCNRLLGIQ
CCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHEEEHHHCCCEEEEE
LTADEMAAIFNRLEFKVNVVEPELFDIDVPSCRVDIEREIDLVEEVARLNGYNNIPVTMP
ECHHHHHHHHHHHEEEEEEECCEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCCCEECC
KARVFSDRPTRHQRLEKQLRNVMVGQGFSEVITFSFMAPGILDKLLLAHDDPRRSVVRLR
CHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHH
NPLVEEQSVMRTTLLPGLLDVAARNINYRMLTLRLFELRRVYLPAEGQELPREPLHLAGI
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
MTGVRYREGWNQERHQVDFYDVKGVIEHILDEFAIGSVVFVQDRLDPYFHPGKACSILCG
HHHHHHHCCCCCHHHEECHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEC
NELLGSFGELHPDIHDNFGIDQSVYYLDLDFEKLAMVSSDNVAIKPPSRFPDTFRDIAML
HHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHEECCCCEEECCCCCCCHHHHHHHHH
IKDETPASTIVECISGLRIREVECAEIFDHYKGSHVPEGFKSIAVRIRYRSHERTLVDNE
HCCCCCHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCEECCCC
VTPLHQRIVDTLVKKLAVTIR
CCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIVTYNWLKEFVDFDLSPQELSDLLTMLGLEVEGVRHVGGGLDEVVVAVVEERSQHPNAD
CEEEHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCC
KLSLCRVNNGKETLAIVCGAQNFKAGDKVALAQIGAVLPGDFKIKRSKIRGEESCGMLCS
CEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCEEEHHHCCCCHHCCCEEC
EKELGLAAESEGIIILPTDLPLGVPVFDALGLKDTIFEIGLTPNRADCLSVVGIAREIAA
CCCCCCEECCCCEEEEECCCCCCCCHHHHCCCHHHHHEECCCCCHHHHHHHHHHHHHHHH
KLGTTVKYARPSVKESVVPISERAQVIVEDSDLCPRYTARYIAGCSIGPSPAWLVRRLEA
HHCCCEECCCCCHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
VGMRSINNVVDVTNYVLMEYGHPLHAFDADLLAGGKIVVRRAAAGERFTTLDGQERVLTE
HHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHCCCCEEEEECCCCCCEEECCCCCCEEEC
SDLTIRDGEKGVALAGIMGGENSEIRQETSNILLESAYFNPSAIRRTSKRLGLHTESSHR
CCCEEECCCCCEEEEEEECCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCCCCC
FERGADVDILVTALDRAASLIAELAGGRVATGTIDVYPRPLPRRTVRFRVDQCNRLLGIQ
CCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHEEEHHHCCCEEEEE
LTADEMAAIFNRLEFKVNVVEPELFDIDVPSCRVDIEREIDLVEEVARLNGYNNIPVTMP
ECHHHHHHHHHHHEEEEEEECCEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCCCEECC
KARVFSDRPTRHQRLEKQLRNVMVGQGFSEVITFSFMAPGILDKLLLAHDDPRRSVVRLR
CHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHH
NPLVEEQSVMRTTLLPGLLDVAARNINYRMLTLRLFELRRVYLPAEGQELPREPLHLAGI
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
MTGVRYREGWNQERHQVDFYDVKGVIEHILDEFAIGSVVFVQDRLDPYFHPGKACSILCG
HHHHHHHCCCCCHHHEECHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEC
NELLGSFGELHPDIHDNFGIDQSVYYLDLDFEKLAMVSSDNVAIKPPSRFPDTFRDIAML
HHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHEECCCCEEECCCCCCCHHHHHHHHH
IKDETPASTIVECISGLRIREVECAEIFDHYKGSHVPEGFKSIAVRIRYRSHERTLVDNE
HCCCCCHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCEECCCC
VTPLHQRIVDTLVKKLAVTIR
CCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA