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The map label for this gene is pheS

Identifier: 78222627

GI number: 78222627

Start: 1582689

End: 1583705

Strand: Direct

Name: pheS

Synonym: Gmet_1415

Alternate gene names: 78222627

Gene position: 1582689-1583705 (Clockwise)

Preceding gene: 78222626

Following gene: 78222628

Centisome position: 39.59

GC content: 56.15

Gene sequence:

>1017_bases
ATGAAAGAACAACTCGACGGACTTCTTCAGTCCGCCCTTAAAGAGATTGATGGGGCTGGGAGCGAAGAGGCTCTCCAGGA
GCTGCGGATAAAATATCTTGGCAAGAAGGGCGCCTTGACAGCCGTCATGAAGGGACTTGGTGCTCTCTCCGCCGAAGAGC
GGCCTGTGGTGGGACAATTGGCCAATACGGTTAAAGACCAACTCGAGGCACACCTTGATGAGACTCTTGCCCAGGTACGC
GACGCCAGCAAGCGCGAGCGGCTTCAGCGCGAGCGTCTTGATGTCACGCTGCCAGGTCGAAGGCTCCCCCATGGCACGAA
ACACCCCATTACGCTGGTCATCGAGGAGATATCGGAGATTTTTGCCGGTCTCGGTTTCCGGGTGGCCGAGGGGCCTGAGG
TTGAACTCGATTTCTATAATTTTGAAGCCCTCAACTTCCCCAAGGACCACCCCGCCAGGGATATGCAGGACACGTTCTTC
GTGGATGATTCCATCCTGCTGAGAACCCATACGTCGCCGGTTCAGGTGCGAACTATGCTCAAACATGCTCCGCCGGTGAG
GATCATTTCTCCGGGGACGGTTTATCGCTGCGACTCCGATGCGACCCATTCCCCCATGTTCCACCAGATCGAGGGATTGA
TGGTCGATACTGGCGTGACCTTCGGTGATCTCAAGGGGATTCTGACTAACTTCGTGAATCAGTATTTCGGCAAGGGAATA
GGGGTCCGTTTGCGGCCGAGCTTTTTCCCCTTCACGGAGCCCAGCGCCGAGGTCGACATTGCCTGCGTTATGTGCAAGGG
GAAAGGCTGTCGCGTCTGCAAAGAGAGCGGATGGCTGGAGATTCTCGGCGCCGGCATGATCGATCCCGAGGTGTACCGTC
ATGTGGGGTATGACCCGGAAACCATTTCCGGTTTCGCTTTCGGCATGGGGGTTGAGCGGGTCGCCATGCTCAAGTACGGC
ATCGGTGATCTGCGTCTTTTCTTTGATAACGATGTCCGTTTCCTGCAGCAATTCTGA

Upstream 100 bases:

>100_bases
TTCTGTCGCCAAAACACAGATTTAGTGGAAATTTCAGCCGGATAAAAAAAGAAATGGGATGCTCAGCGTTCCATTTCTTT
TTTTGTAAAGGGTTCCCGTT

Downstream 100 bases:

>100_bases
AATTACAGTGCATCGGGATTATTCGTTCCAGGTAGAGCAATACTGATTATGTCCCGGATAGGGTGAGAATATGATCGTTA
CGTACAACTGGCTCAAGGAA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKEQLDGLLQSALKEIDGAGSEEALQELRIKYLGKKGALTAVMKGLGALSAEERPVVGQLANTVKDQLEAHLDETLAQVR
DASKRERLQRERLDVTLPGRRLPHGTKHPITLVIEEISEIFAGLGFRVAEGPEVELDFYNFEALNFPKDHPARDMQDTFF
VDDSILLRTHTSPVQVRTMLKHAPPVRIISPGTVYRCDSDATHSPMFHQIEGLMVDTGVTFGDLKGILTNFVNQYFGKGI
GVRLRPSFFPFTEPSAEVDIACVMCKGKGCRVCKESGWLEILGAGMIDPEVYRHVGYDPETISGFAFGMGVERVAMLKYG
IGDLRLFFDNDVRFLQQF

Sequences:

>Translated_338_residues
MKEQLDGLLQSALKEIDGAGSEEALQELRIKYLGKKGALTAVMKGLGALSAEERPVVGQLANTVKDQLEAHLDETLAQVR
DASKRERLQRERLDVTLPGRRLPHGTKHPITLVIEEISEIFAGLGFRVAEGPEVELDFYNFEALNFPKDHPARDMQDTFF
VDDSILLRTHTSPVQVRTMLKHAPPVRIISPGTVYRCDSDATHSPMFHQIEGLMVDTGVTFGDLKGILTNFVNQYFGKGI
GVRLRPSFFPFTEPSAEVDIACVMCKGKGCRVCKESGWLEILGAGMIDPEVYRHVGYDPETISGFAFGMGVERVAMLKYG
IGDLRLFFDNDVRFLQQF
>Mature_338_residues
MKEQLDGLLQSALKEIDGAGSEEALQELRIKYLGKKGALTAVMKGLGALSAEERPVVGQLANTVKDQLEAHLDETLAQVR
DASKRERLQRERLDVTLPGRRLPHGTKHPITLVIEEISEIFAGLGFRVAEGPEVELDFYNFEALNFPKDHPARDMQDTFF
VDDSILLRTHTSPVQVRTMLKHAPPVRIISPGTVYRCDSDATHSPMFHQIEGLMVDTGVTFGDLKGILTNFVNQYFGKGI
GVRLRPSFFPFTEPSAEVDIACVMCKGKGCRVCKESGWLEILGAGMIDPEVYRHVGYDPETISGFAFGMGVERVAMLKYG
IGDLRLFFDNDVRFLQQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=279, Percent_Identity=32.258064516129, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI5729820, Length=289, Percent_Identity=31.1418685121107, Blast_Score=121, Evalue=8e-28,
Organism=Escherichia coli, GI1788007, Length=335, Percent_Identity=51.3432835820895, Blast_Score=352, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=31.021897810219, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=31.021897810219, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32566635, Length=294, Percent_Identity=30.2721088435374, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321087, Length=274, Percent_Identity=30.2919708029197, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=327, Percent_Identity=26.2996941896024, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI18858079, Length=269, Percent_Identity=32.3420074349442, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137424, Length=293, Percent_Identity=29.0102389078498, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_GEOMG (Q39VS5)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384374.1
- ProteinModelPortal:   Q39VS5
- SMR:   Q39VS5
- STRING:   Q39VS5
- GeneID:   3738942
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1415
- NMPDR:   fig|269799.3.peg.1256
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q39VS5
- ProtClustDB:   PRK00488
- BioCyc:   GMET269799:GMET_1415-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37647; Mature: 37647

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEQLDGLLQSALKEIDGAGSEEALQELRIKYLGKKGALTAVMKGLGALSAEERPVVGQL
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHH
ANTVKDQLEAHLDETLAQVRDASKRERLQRERLDVTLPGRRLPHGTKHPITLVIEEISEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHHHH
FAGLGFRVAEGPEVELDFYNFEALNFPKDHPARDMQDTFFVDDSILLRTHTSPVQVRTML
HHCCCCEECCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHH
KHAPPVRIISPGTVYRCDSDATHSPMFHQIEGLMVDTGVTFGDLKGILTNFVNQYFGKGI
HCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCC
GVRLRPSFFPFTEPSAEVDIACVMCKGKGCRVCKESGWLEILGAGMIDPEVYRHVGYDPE
CEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHCCCEEEECCCCCCHHHHHHCCCCCC
TISGFAFGMGVERVAMLKYGIGDLRLFFDNDVRFLQQF
HHCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCC
>Mature Secondary Structure
MKEQLDGLLQSALKEIDGAGSEEALQELRIKYLGKKGALTAVMKGLGALSAEERPVVGQL
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHH
ANTVKDQLEAHLDETLAQVRDASKRERLQRERLDVTLPGRRLPHGTKHPITLVIEEISEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHHHH
FAGLGFRVAEGPEVELDFYNFEALNFPKDHPARDMQDTFFVDDSILLRTHTSPVQVRTML
HHCCCCEECCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHH
KHAPPVRIISPGTVYRCDSDATHSPMFHQIEGLMVDTGVTFGDLKGILTNFVNQYFGKGI
HCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCC
GVRLRPSFFPFTEPSAEVDIACVMCKGKGCRVCKESGWLEILGAGMIDPEVYRHVGYDPE
CEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHCCCEEEECCCCCCHHHHHHCCCCCC
TISGFAFGMGVERVAMLKYGIGDLRLFFDNDVRFLQQF
HHCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA