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The map label for this gene is pilT [H]

Identifier: 78222606

GI number: 78222606

Start: 1561466

End: 1562563

Strand: Direct

Name: pilT [H]

Synonym: Gmet_1394

Alternate gene names: 78222606

Gene position: 1561466-1562563 (Clockwise)

Preceding gene: 78222605

Following gene: 78222607

Centisome position: 39.06

GC content: 61.75

Gene sequence:

>1098_bases
ATGGCCAACATGCATCAGCTGCTCACGGAGCTCGTCAATCGTGGAGGGTCGGACCTCCATCTCACCACCAACTCTCCTCC
CCAGATCCGGATCGACGGGAAACTGCTGCCCCTCGACATGCCACCCCTGAATGCGGTCGATACGAAGCAGCTCTGCTACA
GCATCCTTACCGAGCAGCAGAAGCACAAGTTCGAGGAGAACAACGAGTTGGACCTCTCCTTCGGCATCAAGGGACTGTCA
CGTTTCAGGGGTAACGTCTTTGTCCAGCGGGGGGCCGTGGCCGGGGTGTTCCGGGTTATTCCCTACAAGATCCTCTCCTT
CGAGGAACTGGGGCTCCCGCCGGTGGTGAGGGAGTTGGCCGAGAAGCCCCGGGGCCTTGTCCTCGTCACCGGTCCCACCG
GCAGCGGCAAGTCCACGACCCTGGCCGCCATTATCGACAAGATCAACACCGACCGCCACGAGCACATCGTCACCGTCGAG
GATCCCATCGAGTACCTCCACCCCCACAAGAGCTGCGTGGTGAATCAGCGGGAGGTGGGAGCCGACACCAAGAGCTTCAA
AAACGCCCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTTCTCGTGGGGGAACTCCGGGACCTGGAGACCATCGAGG
CGGCCCTGACCCTGGCCGAAACCGGTCACCTCTGCTTTGCCACGCTCCATACGAACTCCGCGGTCCAGACCATCAACCGG
ATCGTGGATGTGTTCCCCTCCTACCAGCAGCCCCAGGTTCGCGCCCAGCTTTCCTTTGTCCTCGAAGGTGTTCTCTCCCA
AACCCTTCTTCCCAAAGCCAGCGGCACGGGGAGGGTGCTCGCCATCGAGGTCATGGTGCCGAACCCGGCCATCCGCAACC
TGATCCGCGAGGACAAGATCCATCAGATCTACTCCCAGATGCAGGTGGGGCAGGAGAAGTTCGGCATGATGACCATGAAC
CAGTGTCTGTACGGCCTGTTGCAGAAGCGGCACATCACCATGGACGTCGGCATGGGGCGCTCGCCTGATCCCGACGAGCT
CAAGCAGATGCTTACCAGCGGTGTGCGGCCCCAGGCGCCGCGCCCCCCCATGAGATAA

Upstream 100 bases:

>100_bases
TCTCGGCTTCCTCAGCGTACTCTCAGTACGCCTCCGGAGCCTCAACCTCGCCGCCTTGTGCTCATCTCACATCTGACATT
GTGGAATGGAGAACACCATA

Downstream 100 bases:

>100_bases
ACAGGATTCGGAAGGAAGCAGGAGGATAGCAATGCCGAAATTCAGTTGGGAAGCGCGAAGCAGGACCGGAGCGGCTCAGA
AGGGGGTCATGGAGGCCGCG

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MANMHQLLTELVNRGGSDLHLTTNSPPQIRIDGKLLPLDMPPLNAVDTKQLCYSILTEQQKHKFEENNELDLSFGIKGLS
RFRGNVFVQRGAVAGVFRVIPYKILSFEELGLPPVVRELAEKPRGLVLVTGPTGSGKSTTLAAIIDKINTDRHEHIVTVE
DPIEYLHPHKSCVVNQREVGADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCFATLHTNSAVQTINR
IVDVFPSYQQPQVRAQLSFVLEGVLSQTLLPKASGTGRVLAIEVMVPNPAIRNLIREDKIHQIYSQMQVGQEKFGMMTMN
QCLYGLLQKRHITMDVGMGRSPDPDELKQMLTSGVRPQAPRPPMR

Sequences:

>Translated_365_residues
MANMHQLLTELVNRGGSDLHLTTNSPPQIRIDGKLLPLDMPPLNAVDTKQLCYSILTEQQKHKFEENNELDLSFGIKGLS
RFRGNVFVQRGAVAGVFRVIPYKILSFEELGLPPVVRELAEKPRGLVLVTGPTGSGKSTTLAAIIDKINTDRHEHIVTVE
DPIEYLHPHKSCVVNQREVGADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCFATLHTNSAVQTINR
IVDVFPSYQQPQVRAQLSFVLEGVLSQTLLPKASGTGRVLAIEVMVPNPAIRNLIREDKIHQIYSQMQVGQEKFGMMTMN
QCLYGLLQKRHITMDVGMGRSPDPDELKQMLTSGVRPQAPRPPMR
>Mature_364_residues
ANMHQLLTELVNRGGSDLHLTTNSPPQIRIDGKLLPLDMPPLNAVDTKQLCYSILTEQQKHKFEENNELDLSFGIKGLSR
FRGNVFVQRGAVAGVFRVIPYKILSFEELGLPPVVRELAEKPRGLVLVTGPTGSGKSTTLAAIIDKINTDRHEHIVTVED
PIEYLHPHKSCVVNQREVGADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCFATLHTNSAVQTINRI
VDVFPSYQQPQVRAQLSFVLEGVLSQTLLPKASGTGRVLAIEVMVPNPAIRNLIREDKIHQIYSQMQVGQEKFGMMTMNQ
CLYGLLQKRHITMDVGMGRSPDPDELKQMLTSGVRPQAPRPPMR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=319, Percent_Identity=42.9467084639498, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1789723, Length=282, Percent_Identity=34.0425531914894, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786296, Length=288, Percent_Identity=34.375, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40662; Mature: 40531

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANMHQLLTELVNRGGSDLHLTTNSPPQIRIDGKLLPLDMPPLNAVDTKQLCYSILTEQQ
CCCHHHHHHHHHHCCCCEEEEECCCCCEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHH
KHKFEENNELDLSFGIKGLSRFRGNVFVQRGAVAGVFRVIPYKILSFEELGLPPVVRELA
HHHCCCCCCEEEECCHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EKPRGLVLVTGPTGSGKSTTLAAIIDKINTDRHEHIVTVEDPIEYLHPHKSCVVNQREVG
HCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCHHHCCCHHHCC
ADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCFATLHTNSAVQTINR
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
IVDVFPSYQQPQVRAQLSFVLEGVLSQTLLPKASGTGRVLAIEVMVPNPAIRNLIREDKI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
HQIYSQMQVGQEKFGMMTMNQCLYGLLQKRHITMDVGMGRSPDPDELKQMLTSGVRPQAP
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHCCCCCCCC
RPPMR
CCCCC
>Mature Secondary Structure 
ANMHQLLTELVNRGGSDLHLTTNSPPQIRIDGKLLPLDMPPLNAVDTKQLCYSILTEQQ
CCHHHHHHHHHHCCCCEEEEECCCCCEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHH
KHKFEENNELDLSFGIKGLSRFRGNVFVQRGAVAGVFRVIPYKILSFEELGLPPVVRELA
HHHCCCCCCEEEECCHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EKPRGLVLVTGPTGSGKSTTLAAIIDKINTDRHEHIVTVEDPIEYLHPHKSCVVNQREVG
HCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCHHHCCCHHHCC
ADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCFATLHTNSAVQTINR
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
IVDVFPSYQQPQVRAQLSFVLEGVLSQTLLPKASGTGRVLAIEVMVPNPAIRNLIREDKI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
HQIYSQMQVGQEKFGMMTMNQCLYGLLQKRHITMDVGMGRSPDPDELKQMLTSGVRPQAP
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHCCCCCCCC
RPPMR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]