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The map label for this gene is tapB [H]

Identifier: 78222605

GI number: 78222605

Start: 1559620

End: 1561326

Strand: Direct

Name: tapB [H]

Synonym: Gmet_1393

Alternate gene names: 78222605

Gene position: 1559620-1561326 (Clockwise)

Preceding gene: 78222604

Following gene: 78222606

Centisome position: 39.02

GC content: 60.11

Gene sequence:

>1707_bases
ATGCAGGCCAGCAGACTGGGAGAACTCCTGGTTCGAAATAACGTCATCACCAAAGAGCAGCTCGCCAAGGCCCTTGAGGA
GCAGAAGAGCGCAGACGGGCAGCAGCGCCTCGGCTCCATTCTCATCAAGAACGGTCTCATCTCCGAACCTGATCTTACCT
CGTTCCTTTCCAAGCAGTACGGTGTTCCCTCCATCAACCTGAGCGAATTCGAGGCCGAGCAGGCGGTCGTCAAGATCATC
CCCGCCGATGTGGCCCAGAAGTACCAGATCGTCCCGGTGAACAGGGCCGGTTCCACCCTGATCATCGCCATGGCCGACCC
ATCCAATATCTTCGCCATCGATGACATCAAGTTCATGACCGGCTACAACGTGGAGGTGGTGGTGGCCTCCGAGTCGGCCA
TCAAGGCGGCCATCGACAAGTACTACGACCAGTCTGCGTCCCTGGCCGATGTCATGGGCGACCTGGAGATGGACGATCTC
GAGGTGATCGACACCGACGACGAGGTGGATGTATCGTCCCTGGAGCGGGCCACCGAAGACGCTCCGGTCGTGAAGCTCGT
GAACCTGATCCTGACCGACGCCATCAAGCGGAAGGCCAGCGATATCCATATCGAGCCCTACGAGCGGTCGTTCCGGGTCC
GTTACCGGATTGACGGGGTCCTCTACGAGGTGATGAAACCCCCCCTGAAGCTCAAGAACGCCATCACATCCCGAATCAAG
ATCATGGCCGAGCTGGACATCGCCGAACGGCGCCTCCCCCAGGACGGCCGGATCAAGATTAAGCTTGGCGGCGGCCAGGA
CATGGACTACAGGGTATCGGTTCTTCCGACCCTCTTCGGGGAGAAGGTAGTCCTGCGGCTCCTGGATAAGTCGAACTTGC
AGCTGGACATGACCAAGCTGGGTTACGAGCCCGATGCGCTCCACTATTTCAAGGAGGCGATCCACAAGCCCTTCGGCATG
GTGCTCGTCACCGGTCCCACGGGGAGCGGCAAGACGGTCTCCCTATACTCGGCCTTGGGAGAGCTCAACAAGACCACTGA
AAACATCTCCACTGCCGAAGACCCGGTAGAGTTCAACTTTGCCGGCATCAACCAGGTGCAGATGCACGAGGATATCGGCC
TCAACTTCGCCGCTGCTCTCCGCTCATTTCTGCGCCAGGACCCTGACATTATCATGATCGGCGAGATCCGTGACTTCGAG
ACCGCCGAGATCGCCATCAAGGCGGCCCTCACCGGGCACCTGGTCCTCTCCACCCTTCACACCAACGATGCCCCGGCCAC
CATCAACCGCCTCCTCAACATGGGGGTCGAGCCGTTCCTGGTGGCGTCGGCCGTGAACCTGATTACCGCCCAGCGTCTTG
CCCGCCGGGTCTGCTCCGAGTGCAAACAGCCGGAAGAGATCCCGATCCAGGCCCTCATCGATGCCGGTGTTTCCCCCGAC
GAGGCGCCGAGCTACGTCTGCTACAAGGGGACCGGCTGCGTCAAGTGCAACAACACCGGCTACAAGGGACGGGTTGGCTT
CTATCAGGTCATGCCGATGCTGGAGGAGATCCGGGAGTTGATCCTTAACGGCGCCAATACCGCGGAGATCAAGCGGGAGT
CCATGAGGCTCGGGATCAAGACCATGCGCCAGTCGGGGCTTACCAAGCTGAAAGAGGGCGTTACGTCCTTTGAGGAGGTG
CTGCGGGTCACCGTGGCCGACGATTAA

Upstream 100 bases:

>100_bases
CATGAGGAGACGTGTTCTGGCAGCCCTGAAGCGCTAACCACTTCTTGACATTGGGGTTTTGCCACCCTACCATTACGGCG
ACTTTTTCCGCGGAGAATCT

Downstream 100 bases:

>100_bases
ACACAATGTCATATTTGAGCGATCACGGCGTTGGCTCGGTCTCGGCTTCCTCAGCGTACTCTCAGTACGCCTCCGGAGCC
TCAACCTCGCCGCCTTGTGC

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MQASRLGELLVRNNVITKEQLAKALEEQKSADGQQRLGSILIKNGLISEPDLTSFLSKQYGVPSINLSEFEAEQAVVKII
PADVAQKYQIVPVNRAGSTLIIAMADPSNIFAIDDIKFMTGYNVEVVVASESAIKAAIDKYYDQSASLADVMGDLEMDDL
EVIDTDDEVDVSSLERATEDAPVVKLVNLILTDAIKRKASDIHIEPYERSFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
IMAELDIAERRLPQDGRIKIKLGGGQDMDYRVSVLPTLFGEKVVLRLLDKSNLQLDMTKLGYEPDALHYFKEAIHKPFGM
VLVTGPTGSGKTVSLYSALGELNKTTENISTAEDPVEFNFAGINQVQMHEDIGLNFAAALRSFLRQDPDIIMIGEIRDFE
TAEIAIKAALTGHLVLSTLHTNDAPATINRLLNMGVEPFLVASAVNLITAQRLARRVCSECKQPEEIPIQALIDAGVSPD
EAPSYVCYKGTGCVKCNNTGYKGRVGFYQVMPMLEEIRELILNGANTAEIKRESMRLGIKTMRQSGLTKLKEGVTSFEEV
LRVTVADD

Sequences:

>Translated_568_residues
MQASRLGELLVRNNVITKEQLAKALEEQKSADGQQRLGSILIKNGLISEPDLTSFLSKQYGVPSINLSEFEAEQAVVKII
PADVAQKYQIVPVNRAGSTLIIAMADPSNIFAIDDIKFMTGYNVEVVVASESAIKAAIDKYYDQSASLADVMGDLEMDDL
EVIDTDDEVDVSSLERATEDAPVVKLVNLILTDAIKRKASDIHIEPYERSFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
IMAELDIAERRLPQDGRIKIKLGGGQDMDYRVSVLPTLFGEKVVLRLLDKSNLQLDMTKLGYEPDALHYFKEAIHKPFGM
VLVTGPTGSGKTVSLYSALGELNKTTENISTAEDPVEFNFAGINQVQMHEDIGLNFAAALRSFLRQDPDIIMIGEIRDFE
TAEIAIKAALTGHLVLSTLHTNDAPATINRLLNMGVEPFLVASAVNLITAQRLARRVCSECKQPEEIPIQALIDAGVSPD
EAPSYVCYKGTGCVKCNNTGYKGRVGFYQVMPMLEEIRELILNGANTAEIKRESMRLGIKTMRQSGLTKLKEGVTSFEEV
LRVTVADD
>Mature_568_residues
MQASRLGELLVRNNVITKEQLAKALEEQKSADGQQRLGSILIKNGLISEPDLTSFLSKQYGVPSINLSEFEAEQAVVKII
PADVAQKYQIVPVNRAGSTLIIAMADPSNIFAIDDIKFMTGYNVEVVVASESAIKAAIDKYYDQSASLADVMGDLEMDDL
EVIDTDDEVDVSSLERATEDAPVVKLVNLILTDAIKRKASDIHIEPYERSFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
IMAELDIAERRLPQDGRIKIKLGGGQDMDYRVSVLPTLFGEKVVLRLLDKSNLQLDMTKLGYEPDALHYFKEAIHKPFGM
VLVTGPTGSGKTVSLYSALGELNKTTENISTAEDPVEFNFAGINQVQMHEDIGLNFAAALRSFLRQDPDIIMIGEIRDFE
TAEIAIKAALTGHLVLSTLHTNDAPATINRLLNMGVEPFLVASAVNLITAQRLARRVCSECKQPEEIPIQALIDAGVSPD
EAPSYVCYKGTGCVKCNNTGYKGRVGFYQVMPMLEEIRELILNGANTAEIKRESMRLGIKTMRQSGLTKLKEGVTSFEEV
LRVTVADD

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=421, Percent_Identity=45.8432304038005, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=46.5968586387435, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI87082188, Length=251, Percent_Identity=34.2629482071713, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62659; Mature: 62659

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASRLGELLVRNNVITKEQLAKALEEQKSADGQQRLGSILIKNGLISEPDLTSFLSKQY
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHC
GVPSINLSEFEAEQAVVKIIPADVAQKYQIVPVNRAGSTLIIAMADPSNIFAIDDIKFMT
CCCCCCHHHHHHHHHHHEEECHHHHCCEEEEEECCCCCEEEEEEECCCCEEEEECEEEEC
GYNVEVVVASESAIKAAIDKYYDQSASLADVMGDLEMDDLEVIDTDDEVDVSSLERATED
CCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEECCCCCCCHHHHHHHCCC
APVVKLVNLILTDAIKRKASDIHIEPYERSFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
CHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHH
IMAELDIAERRLPQDGRIKIKLGGGQDMDYRVSVLPTLFGEKVVLRLLDKSNLQLDMTKL
HHHHCCHHHHHCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEHHHC
GYEPDALHYFKEAIHKPFGMVLVTGPTGSGKTVSLYSALGELNKTTENISTAEDPVEFNF
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEEE
AGINQVQMHEDIGLNFAAALRSFLRQDPDIIMIGEIRDFETAEIAIKAALTGHLVLSTLH
CCCCEEEEHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHEEEEHHHHHHEEEEEEC
TNDAPATINRLLNMGVEPFLVASAVNLITAQRLARRVCSECKQPEEIPIQALIDAGVSPD
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCC
EAPSYVCYKGTGCVKCNNTGYKGRVGFYQVMPMLEEIRELILNGANTAEIKRESMRLGIK
CCCCEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TMRQSGLTKLKEGVTSFEEVLRVTVADD
HHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQASRLGELLVRNNVITKEQLAKALEEQKSADGQQRLGSILIKNGLISEPDLTSFLSKQY
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHC
GVPSINLSEFEAEQAVVKIIPADVAQKYQIVPVNRAGSTLIIAMADPSNIFAIDDIKFMT
CCCCCCHHHHHHHHHHHEEECHHHHCCEEEEEECCCCCEEEEEEECCCCEEEEECEEEEC
GYNVEVVVASESAIKAAIDKYYDQSASLADVMGDLEMDDLEVIDTDDEVDVSSLERATED
CCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEECCCCCCCHHHHHHHCCC
APVVKLVNLILTDAIKRKASDIHIEPYERSFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
CHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHH
IMAELDIAERRLPQDGRIKIKLGGGQDMDYRVSVLPTLFGEKVVLRLLDKSNLQLDMTKL
HHHHCCHHHHHCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEHHHC
GYEPDALHYFKEAIHKPFGMVLVTGPTGSGKTVSLYSALGELNKTTENISTAEDPVEFNF
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEEE
AGINQVQMHEDIGLNFAAALRSFLRQDPDIIMIGEIRDFETAEIAIKAALTGHLVLSTLH
CCCCEEEEHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHEEEEHHHHHHEEEEEEC
TNDAPATINRLLNMGVEPFLVASAVNLITAQRLARRVCSECKQPEEIPIQALIDAGVSPD
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCC
EAPSYVCYKGTGCVKCNNTGYKGRVGFYQVMPMLEEIRELILNGANTAEIKRESMRLGIK
CCCCEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TMRQSGLTKLKEGVTSFEEVLRVTVADD
HHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]