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The map label for this gene is emrB [H]

Identifier: 78222586

GI number: 78222586

Start: 1535207

End: 1536760

Strand: Reverse

Name: emrB [H]

Synonym: Gmet_1374

Alternate gene names: 78222586

Gene position: 1536760-1535207 (Counterclockwise)

Preceding gene: 78222587

Following gene: 78222562

Centisome position: 38.44

GC content: 60.94

Gene sequence:

>1554_bases
ATGGACGCACCGGTCAAGGACGTCAACAAGTGGATCGTCACCATCACCGTGATGCTTCCCACCATCATGGAGATCGTAGA
CACGTCGGTGGCCAACGTGGCGCTTCCCCACATGCAGGGGAGCCTCAACGCGGGGACCGACGAAATCACGTGGGTCCTCA
CCTCCTACCTGGTGAGCAATGCCGTGGTCCTTCCCATGACCGGCTGGCTTTCCAGGCTTTTCGGACGCAAACGGTTTCTC
ATCTCCTGCATCACCCTCTTCACTCTGGCCTCGCTTCTCTGCGGCGCGGCCCCCAACCTGGCAAGCCTCATCTTTTTCCG
GGTGCTCCAGGGAGCGGCCGGGGGCGCCCTCATCCCCAGCAGCCAGGCGATCCTCCTGGAAACGTTTCCTCCCCACGAAC
GGGGAATGGCCAATGCCATCTTCGGCGTAGGCGCCATGTTCGGGCCGATCATCGGCCCGGCCCTCGGAGGGTGGATCACC
GACAACCTGAACTGGCGGTGGATCTTCTATATCAACATCCCCCTCGGCATCATCGCCGTCATCATGGCCGCCGCCTTCAT
CTTCGACCCGCCGTACCTGCGCCGGGCCAGGGTGGCCATTGACTGGTGGGGGCTCGGGCTTCTCACCGTCGGTCTCGGAG
CGCTCCAGATCGTCCTCGACAAAGGGCAGCAGGACGACTGGTTCAATTCATCCTTCATCCTGTCCTGCTCGATCCTCTCG
GCCCTGACCCTCCTCGCCCTCGTCTATGTGGAACTGAAACACGAACACCCGATCATCAACCTGCGGCTCTTCAAGGATGT
CTCCTTCTCGGCGGGGAACTTCGTCATGTTCATGCTGGGCTTCAGTCTCTACAGCTCCATCGTGCTGATCCCCCTCTTCC
TCCAGACCCTCATGGGGTACGACGCCACCATGGCGGGGATGGTCCTTGCCCCGGGGGGAGCGGCTACCCTCATCACCATG
CCCTTCGTCGGCGCGGTTATCTCGAAATATGATGGGAGAAAGGTGATACTGGTGGGGCTCATCATCAGCGCCTGGTCCAT
GTTCCTGATGCGGGGCTTCACGCTGGAGGCGTCGTACTGGGACTTCGTCTGGCCCAGGGTCATTCTCGGGGTGGGGCTTG
CCATGCTCTTCGTGCCGCTCTCCACCCTGACCCTCGGCTCGATTCCGAAGGTGGAAATGGGAAATGCCGCCGGCCTTTAC
AATCTGCTGCGCAACATGGGCGGCAGTGTCGGGATCGCCGTGGCGGCAACGATGCTCTCCCGCCAGTCTCAATTTTATCA
GAATGTCCTGGCTGGACAGGTTACACCCTACAGCCCGGTGGCCCGGCAAAAACTTGAGATGGTGAAGCAGGGATTGATGC
TGAGGGGCCTCGATGCCGCCACCGCCGACAAGGCGTCCCTGGCAGCCATCTATGGCATGCTCCAGCGCCAGGCGGGGATG
CTGGCCTACAACCGTATTTTCTGGATCGTGGGATTGACGTTTCTCGGTGTGATGCCGTTTCTGCTCCTGCTCAGAAAGCA
GAAAACCGGCGCTGAAACCGAAGGGGTGCACTAA

Upstream 100 bases:

>100_bases
TCTGCTCCGGGTGGGAATGAGCGTGATCCCCACCGTGAACACCGGGCGCAAACTGGGGGAAATCCTCAGGGATTTCCGGC
CCTTCTAGGACTGCGGGTTC

Downstream 100 bases:

>100_bases
CGCTCGGTGAGAATCTCATCCTGCAACTCGTTCACATCGTCGGCCCTGTGGGGGAAATGCCTGGCCAGTTCGGCGCCGCA
CCCGGCAACGACCGAGCAGA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MDAPVKDVNKWIVTITVMLPTIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPMTGWLSRLFGRKRFL
ISCITLFTLASLLCGAAPNLASLIFFRVLQGAAGGALIPSSQAILLETFPPHERGMANAIFGVGAMFGPIIGPALGGWIT
DNLNWRWIFYINIPLGIIAVIMAAAFIFDPPYLRRARVAIDWWGLGLLTVGLGALQIVLDKGQQDDWFNSSFILSCSILS
ALTLLALVYVELKHEHPIINLRLFKDVSFSAGNFVMFMLGFSLYSSIVLIPLFLQTLMGYDATMAGMVLAPGGAATLITM
PFVGAVISKYDGRKVILVGLIISAWSMFLMRGFTLEASYWDFVWPRVILGVGLAMLFVPLSTLTLGSIPKVEMGNAAGLY
NLLRNMGGSVGIAVAATMLSRQSQFYQNVLAGQVTPYSPVARQKLEMVKQGLMLRGLDAATADKASLAAIYGMLQRQAGM
LAYNRIFWIVGLTFLGVMPFLLLLRKQKTGAETEGVH

Sequences:

>Translated_517_residues
MDAPVKDVNKWIVTITVMLPTIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPMTGWLSRLFGRKRFL
ISCITLFTLASLLCGAAPNLASLIFFRVLQGAAGGALIPSSQAILLETFPPHERGMANAIFGVGAMFGPIIGPALGGWIT
DNLNWRWIFYINIPLGIIAVIMAAAFIFDPPYLRRARVAIDWWGLGLLTVGLGALQIVLDKGQQDDWFNSSFILSCSILS
ALTLLALVYVELKHEHPIINLRLFKDVSFSAGNFVMFMLGFSLYSSIVLIPLFLQTLMGYDATMAGMVLAPGGAATLITM
PFVGAVISKYDGRKVILVGLIISAWSMFLMRGFTLEASYWDFVWPRVILGVGLAMLFVPLSTLTLGSIPKVEMGNAAGLY
NLLRNMGGSVGIAVAATMLSRQSQFYQNVLAGQVTPYSPVARQKLEMVKQGLMLRGLDAATADKASLAAIYGMLQRQAGM
LAYNRIFWIVGLTFLGVMPFLLLLRKQKTGAETEGVH
>Mature_517_residues
MDAPVKDVNKWIVTITVMLPTIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPMTGWLSRLFGRKRFL
ISCITLFTLASLLCGAAPNLASLIFFRVLQGAAGGALIPSSQAILLETFPPHERGMANAIFGVGAMFGPIIGPALGGWIT
DNLNWRWIFYINIPLGIIAVIMAAAFIFDPPYLRRARVAIDWWGLGLLTVGLGALQIVLDKGQQDDWFNSSFILSCSILS
ALTLLALVYVELKHEHPIINLRLFKDVSFSAGNFVMFMLGFSLYSSIVLIPLFLQTLMGYDATMAGMVLAPGGAATLITM
PFVGAVISKYDGRKVILVGLIISAWSMFLMRGFTLEASYWDFVWPRVILGVGLAMLFVPLSTLTLGSIPKVEMGNAAGLY
NLLRNMGGSVGIAVAATMLSRQSQFYQNVLAGQVTPYSPVARQKLEMVKQGLMLRGLDAATADKASLAAIYGMLQRQAGM
LAYNRIFWIVGLTFLGVMPFLLLLRKQKTGAETEGVH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=497, Percent_Identity=36.0160965794769, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1788710, Length=498, Percent_Identity=35.9437751004016, Blast_Score=291, Evalue=1e-79,
Organism=Escherichia coli, GI1790195, Length=407, Percent_Identity=28.992628992629, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87081983, Length=431, Percent_Identity=26.6821345707657, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788392, Length=427, Percent_Identity=28.3372365339578, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87082320, Length=200, Percent_Identity=25.5, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=387, Percent_Identity=26.0981912144703, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=137, Percent_Identity=30.6569343065693, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=526, Percent_Identity=21.1026615969582, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324954, Length=397, Percent_Identity=23.6775818639798, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=132, Percent_Identity=32.5757575757576, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=133, Percent_Identity=30.8270676691729, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56189; Mature: 56189

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAPVKDVNKWIVTITVMLPTIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
AVVLPMTGWLSRLFGRKRFLISCITLFTLASLLCGAAPNLASLIFFRVLQGAAGGALIPS
CEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCC
SQAILLETFPPHERGMANAIFGVGAMFGPIIGPALGGWITDNLNWRWIFYINIPLGIIAV
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEEECCHHHHHH
IMAAAFIFDPPYLRRARVAIDWWGLGLLTVGLGALQIVLDKGQQDDWFNSSFILSCSILS
HHHHHHHCCCHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ALTLLALVYVELKHEHPIINLRLFKDVSFSAGNFVMFMLGFSLYSSIVLIPLFLQTLMGY
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DATMAGMVLAPGGAATLITMPFVGAVISKYDGRKVILVGLIISAWSMFLMRGFTLEASYW
CCHHHCEEECCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCEEECCHH
DFVWPRVILGVGLAMLFVPLSTLTLGSIPKVEMGNAAGLYNLLRNMGGSVGIAVAATMLS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
RQSQFYQNVLAGQVTPYSPVARQKLEMVKQGLMLRGLDAATADKASLAAIYGMLQRQAGM
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHCCH
LAYNRIFWIVGLTFLGVMPFLLLLRKQKTGAETEGVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDAPVKDVNKWIVTITVMLPTIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
AVVLPMTGWLSRLFGRKRFLISCITLFTLASLLCGAAPNLASLIFFRVLQGAAGGALIPS
CEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCC
SQAILLETFPPHERGMANAIFGVGAMFGPIIGPALGGWITDNLNWRWIFYINIPLGIIAV
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEEECCHHHHHH
IMAAAFIFDPPYLRRARVAIDWWGLGLLTVGLGALQIVLDKGQQDDWFNSSFILSCSILS
HHHHHHHCCCHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ALTLLALVYVELKHEHPIINLRLFKDVSFSAGNFVMFMLGFSLYSSIVLIPLFLQTLMGY
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DATMAGMVLAPGGAATLITMPFVGAVISKYDGRKVILVGLIISAWSMFLMRGFTLEASYW
CCHHHCEEECCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCEEECCHH
DFVWPRVILGVGLAMLFVPLSTLTLGSIPKVEMGNAAGLYNLLRNMGGSVGIAVAATMLS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
RQSQFYQNVLAGQVTPYSPVARQKLEMVKQGLMLRGLDAATADKASLAAIYGMLQRQAGM
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHCCH
LAYNRIFWIVGLTFLGVMPFLLLLRKQKTGAETEGVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]