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The map label for this gene is ispG

Identifier: 78222565

GI number: 78222565

Start: 1514414

End: 1515481

Strand: Direct

Name: ispG

Synonym: Gmet_1353

Alternate gene names: 78222565

Gene position: 1514414-1515481 (Clockwise)

Preceding gene: 78222564

Following gene: 78222566

Centisome position: 37.88

GC content: 63.48

Gene sequence:

>1068_bases
ATGATACGCGAAACCAGGCAGATTCACGTAGGAAATGTCGCCATCGGCGGGGGAGCCCCCTGTTCGGTGCAGTCCATGTG
CAACACCGATACCCGCGACGTGGCAGCCACCCTTTCCCAGGTTCTCTCCCTTTCATCGGCGGGTTGTGAGATCGTCCGCT
GCGCCGTGCCCGACATGGCGGCGGCCGAAGCGCTGGGTGAGATCAAGCGGCAGAGCCCGATCCCGGTCATCGCCGACATC
CACTTCGATTATCGCCTTGCCCTGCGGGTGCTCGAAGGGGGGATTGACGGCCTGCGCCTCAACCCCGGCAATATCGGCGA
GCGGTGGAAGGTGGAAGAGGTGGTGAAGGCGGCCCGGGAGCGCCTCGTCCCGATCCGGATCGGTGTCAATGCCGGTTCCC
TGGAGAAGGAGCTCCTCGAAAAGTACGGCCATCCCACCGCCGAGGCCATGGTTGAGTCGGCCCTGGGCCATGTCCGCATC
CTCGAGGATATCGGCTACGACCAGATCAAGATCTCCCTCAAGGCCTCCGACGTCATGAAGACCGTGGAAGCCTACCGGCT
CCTGGCTCAGAAGGTCGATTACCCTCTCCACATCGGCATCACCGAAGCCGGCACCATCTTCTCCGGCACCATCAAGTCCT
CCGTGGGGCTCGGCATCCTCCTGGCCGACGGCATCGGCGACACCATGCGCGTATCGCTCACCGGCGATCCCGTGGACGAA
GTGCGGGTCGGCTTCGAGATTCTCAAGGCCCTGGGGTTGCGGCAGCGGGGAATCAATTTCGTCTCCTGTCCCACCTGCGG
CCGTTGCCAGATCAATCTGATCGGCGTCGCCCAGGAGGTGGAGAACCGCCTCGCCGCCATCGATACTCCTCTGACCGTGG
CTGTCATGGGGTGTGTGGTGAACGGTCCGGGCGAGGCGCGGGAGGCCGATGTGGGGATCGCCGGAGGGAAGGGTGAAGGA
CTCCTTTTCCGGCATGGCGAGATCGTCCGCAAGGTCCCCGAAGCCGAGATGGCCGATGCCTTGATCGCCGAGGTGGAGCA
AATGGCCAAGGAAAAAACACACTCATAG

Upstream 100 bases:

>100_bases
AGGCGGGAGCGATCCCGCCTTTTTTTTATCCTGTAGCCCCATGTTATGAAGCCAAACCGTTCTTGACTTTCCGCGGCCGT
TCCGGCTACTATGCTCCGCT

Downstream 100 bases:

>100_bases
AGGTTCGTTTAATGCGCTATTCCCAGTATTTCATCCCCACGGTCAAGGAGACTCCTTCCGACGCCGAGGTCGTTTCCCAC
AAGCTCATGCTCCGTGCTGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MIRETRQIHVGNVAIGGGAPCSVQSMCNTDTRDVAATLSQVLSLSSAGCEIVRCAVPDMAAAEALGEIKRQSPIPVIADI
HFDYRLALRVLEGGIDGLRLNPGNIGERWKVEEVVKAARERLVPIRIGVNAGSLEKELLEKYGHPTAEAMVESALGHVRI
LEDIGYDQIKISLKASDVMKTVEAYRLLAQKVDYPLHIGITEAGTIFSGTIKSSVGLGILLADGIGDTMRVSLTGDPVDE
VRVGFEILKALGLRQRGINFVSCPTCGRCQINLIGVAQEVENRLAAIDTPLTVAVMGCVVNGPGEAREADVGIAGGKGEG
LLFRHGEIVRKVPEAEMADALIAEVEQMAKEKTHS

Sequences:

>Translated_355_residues
MIRETRQIHVGNVAIGGGAPCSVQSMCNTDTRDVAATLSQVLSLSSAGCEIVRCAVPDMAAAEALGEIKRQSPIPVIADI
HFDYRLALRVLEGGIDGLRLNPGNIGERWKVEEVVKAARERLVPIRIGVNAGSLEKELLEKYGHPTAEAMVESALGHVRI
LEDIGYDQIKISLKASDVMKTVEAYRLLAQKVDYPLHIGITEAGTIFSGTIKSSVGLGILLADGIGDTMRVSLTGDPVDE
VRVGFEILKALGLRQRGINFVSCPTCGRCQINLIGVAQEVENRLAAIDTPLTVAVMGCVVNGPGEAREADVGIAGGKGEG
LLFRHGEIVRKVPEAEMADALIAEVEQMAKEKTHS
>Mature_355_residues
MIRETRQIHVGNVAIGGGAPCSVQSMCNTDTRDVAATLSQVLSLSSAGCEIVRCAVPDMAAAEALGEIKRQSPIPVIADI
HFDYRLALRVLEGGIDGLRLNPGNIGERWKVEEVVKAARERLVPIRIGVNAGSLEKELLEKYGHPTAEAMVESALGHVRI
LEDIGYDQIKISLKASDVMKTVEAYRLLAQKVDYPLHIGITEAGTIFSGTIKSSVGLGILLADGIGDTMRVSLTGDPVDE
VRVGFEILKALGLRQRGINFVSCPTCGRCQINLIGVAQEVENRLAAIDTPLTVAVMGCVVNGPGEAREADVGIAGGKGEG
LLFRHGEIVRKVPEAEMADALIAEVEQMAKEKTHS

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=349, Percent_Identity=53.5816618911175, Blast_Score=390, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 37847; Mature: 37847

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRETRQIHVGNVAIGGGAPCSVQSMCNTDTRDVAATLSQVLSLSSAGCEIVRCAVPDMA
CCCCCCEEEECEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
AAEALGEIKRQSPIPVIADIHFDYRLALRVLEGGIDGLRLNPGNIGERWKVEEVVKAARE
HHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHH
RLVPIRIGVNAGSLEKELLEKYGHPTAEAMVESALGHVRILEDIGYDQIKISLKASDVMK
CCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEHHHCCCCEEEEEEEHHHHHH
TVEAYRLLAQKVDYPLHIGITEAGTIFSGTIKSSVGLGILLADGIGDTMRVSLTGDPVDE
HHHHHHHHHHHCCCCEEEEEECCCCEEECCHHHCCCEEEEEECCCCCEEEEEECCCCHHH
VRVGFEILKALGLRQRGINFVSCPTCGRCQINLIGVAQEVENRLAAIDTPLTVAVMGCVV
HHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHC
NGPGEAREADVGIAGGKGEGLLFRHGEIVRKVPEAEMADALIAEVEQMAKEKTHS
CCCCCCCCCCCEEECCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRETRQIHVGNVAIGGGAPCSVQSMCNTDTRDVAATLSQVLSLSSAGCEIVRCAVPDMA
CCCCCCEEEECEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
AAEALGEIKRQSPIPVIADIHFDYRLALRVLEGGIDGLRLNPGNIGERWKVEEVVKAARE
HHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHH
RLVPIRIGVNAGSLEKELLEKYGHPTAEAMVESALGHVRILEDIGYDQIKISLKASDVMK
CCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEHHHCCCCEEEEEEEHHHHHH
TVEAYRLLAQKVDYPLHIGITEAGTIFSGTIKSSVGLGILLADGIGDTMRVSLTGDPVDE
HHHHHHHHHHHCCCCEEEEEECCCCEEECCHHHCCCEEEEEECCCCCEEEEEECCCCHHH
VRVGFEILKALGLRQRGINFVSCPTCGRCQINLIGVAQEVENRLAAIDTPLTVAVMGCVV
HHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHC
NGPGEAREADVGIAGGKGEGLLFRHGEIVRKVPEAEMADALIAEVEQMAKEKTHS
CCCCCCCCCCCEEECCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA