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The map label for this gene is glnA [H]

Identifier: 78222564

GI number: 78222564

Start: 1512872

End: 1514284

Strand: Direct

Name: glnA [H]

Synonym: Gmet_1352

Alternate gene names: 78222564

Gene position: 1512872-1514284 (Clockwise)

Preceding gene: 78222563

Following gene: 78222565

Centisome position: 37.85

GC content: 59.59

Gene sequence:

>1413_bases
ATGACACCGCAAGAAGTAGTAAGCTTCGCCAAGGAAAACGGCGCGCTGATGGTTGATTTCAAGTTCATGGATTTCGTGGG
GACATGGCAGCACATTTCCGTACCCATCACGGAGTTCAGCGAAGATACCTTCGAAGAGGGGCAGGGGTTTGACGGCTCTT
CCATCCGTGGCTGGCAGCCGATCCACGCCTCCGACATGATCCTTCTCCCCGATCCCGACACGGCCAAGATGGACCCCTTC
GTGGCGGTGCCGACCCTGTCGCTGATCTGCAATGTCTTCGATCCGATCACCAAGGAAGACTACACCCGCGATCCCCGGAA
CATCGCCCGCAAGGCCGAGGCCTACCTCAAGTCCACCGGCATCGGCGAAATCGCCTTCTTCGGTCCGGAAGCCGAGTTCT
TCATCTTCGACGAGGTCCGTTACGATTCCAGCTCCAATCAGTCCTTCTACATGGTCGATTCCGTGGAAGGTGCCTGGAAC
TCCGGCCGTGAAGAGTTCCCGAACCTGGGCTACAAGCCGCGCCACAAGGAAGGGTACTTCCCCCTCTCCCCCACCGACTC
CCAGAACGATCTCCGCAACGAGATGGTGATGGAGCTCCAGAAGGTCGGCATCCGGGTCGAGTGCCAGCACCACGAGGTTG
CCACTGGCGGCCAGGCCGAGATCGACATGCGTTTCTCTTCCCTGGTGGACATGGCTGACCAGCTCCAGTGGTTCAAGTAT
GTCATCAAGAACGTTGCCTACAGAAACAGCAAGACCGTAACCTTCATGCCGAAGCCCCTGTACGGCGACAACGGTTCCGG
CATGCACTGCCACCAGTCCATCTGGAAGAACGGCCAGAACCTCTTCGCCGGCGACAAGTACGGCGGGCTCTCCCAGATGG
CCCTCTGGTACATCGGCGGCATCATCAAGCACGCCAAGGCCCTCTGTGCCATCACCAACCCGACCACCAACTCCTACAAG
CGTCTGGTGCCGGGCTTCGAGGCCCCGGTTAACATGGCATACTCCAGCCGTAACCGCTCGGCATCCATCCGGATCCCGAT
GCTCTCCACCAACCCGAAGGCCAAGCGGATCGAATACCGGACCCCTGACCCCTCCTGCAACGGCTACCTCGCCTTCGCCG
CCATGCTCATGGCCGGCCTCGACGGCATCGAGAACAAGATTGATCCGGGGCAGCCTCTCGATAAGGATATCTACGGACTT
TCCCCCGAAGAGCTCAAGGACATTCCGTCCGCTCCCGGGACCCTTGAAGACGCCCTCAACGCCCTCAGGGACGACCACGA
GTTTCTTCTCAAGGGTGACGTCTTCACCCCCGACGTCATCGAGAAGTGGATCGAGTACAAGATGGAGGCCGAAGTCAACC
CGGTCCGGATGCGTCCGGTTCCCCTTGAGTTCGCCCTCTACTACGACATCTAG

Upstream 100 bases:

>100_bases
GAGGAAGTGATCCGCATCCGCACCGGTGAGCGGGGCGAGGACGCCCTGTAGAATCACTACTATACGCCAGGTAGTTTTAC
CATTGAAAGGAGACGTTTTG

Downstream 100 bases:

>100_bases
GCCCATAGGCGGTACTGCATCATCACGAAAGGCGGGAGCGATCCCGCCTTTTTTTTATCCTGTAGCCCCATGTTATGAAG
CCAAACCGTTCTTGACTTTC

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTPQEVVSFAKENGALMVDFKFMDFVGTWQHISVPITEFSEDTFEEGQGFDGSSIRGWQPIHASDMILLPDPDTAKMDPF
VAVPTLSLICNVFDPITKEDYTRDPRNIARKAEAYLKSTGIGEIAFFGPEAEFFIFDEVRYDSSSNQSFYMVDSVEGAWN
SGREEFPNLGYKPRHKEGYFPLSPTDSQNDLRNEMVMELQKVGIRVECQHHEVATGGQAEIDMRFSSLVDMADQLQWFKY
VIKNVAYRNSKTVTFMPKPLYGDNGSGMHCHQSIWKNGQNLFAGDKYGGLSQMALWYIGGIIKHAKALCAITNPTTNSYK
RLVPGFEAPVNMAYSSRNRSASIRIPMLSTNPKAKRIEYRTPDPSCNGYLAFAAMLMAGLDGIENKIDPGQPLDKDIYGL
SPEELKDIPSAPGTLEDALNALRDDHEFLLKGDVFTPDVIEKWIEYKMEAEVNPVRMRPVPLEFALYYDI

Sequences:

>Translated_470_residues
MTPQEVVSFAKENGALMVDFKFMDFVGTWQHISVPITEFSEDTFEEGQGFDGSSIRGWQPIHASDMILLPDPDTAKMDPF
VAVPTLSLICNVFDPITKEDYTRDPRNIARKAEAYLKSTGIGEIAFFGPEAEFFIFDEVRYDSSSNQSFYMVDSVEGAWN
SGREEFPNLGYKPRHKEGYFPLSPTDSQNDLRNEMVMELQKVGIRVECQHHEVATGGQAEIDMRFSSLVDMADQLQWFKY
VIKNVAYRNSKTVTFMPKPLYGDNGSGMHCHQSIWKNGQNLFAGDKYGGLSQMALWYIGGIIKHAKALCAITNPTTNSYK
RLVPGFEAPVNMAYSSRNRSASIRIPMLSTNPKAKRIEYRTPDPSCNGYLAFAAMLMAGLDGIENKIDPGQPLDKDIYGL
SPEELKDIPSAPGTLEDALNALRDDHEFLLKGDVFTPDVIEKWIEYKMEAEVNPVRMRPVPLEFALYYDI
>Mature_469_residues
TPQEVVSFAKENGALMVDFKFMDFVGTWQHISVPITEFSEDTFEEGQGFDGSSIRGWQPIHASDMILLPDPDTAKMDPFV
AVPTLSLICNVFDPITKEDYTRDPRNIARKAEAYLKSTGIGEIAFFGPEAEFFIFDEVRYDSSSNQSFYMVDSVEGAWNS
GREEFPNLGYKPRHKEGYFPLSPTDSQNDLRNEMVMELQKVGIRVECQHHEVATGGQAEIDMRFSSLVDMADQLQWFKYV
IKNVAYRNSKTVTFMPKPLYGDNGSGMHCHQSIWKNGQNLFAGDKYGGLSQMALWYIGGIIKHAKALCAITNPTTNSYKR
LVPGFEAPVNMAYSSRNRSASIRIPMLSTNPKAKRIEYRTPDPSCNGYLAFAAMLMAGLDGIENKIDPGQPLDKDIYGLS
PEELKDIPSAPGTLEDALNALRDDHEFLLKGDVFTPDVIEKWIEYKMEAEVNPVRMRPVPLEFALYYDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=375, Percent_Identity=25.0666666666667, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1790301, Length=471, Percent_Identity=55.4140127388535, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI87081870, Length=188, Percent_Identity=30.3191489361702, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52977; Mature: 52845

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQEVVSFAKENGALMVDFKFMDFVGTWQHISVPITEFSEDTFEEGQGFDGSSIRGWQP
CCHHHHHHHHHCCCCEEEEEEHHHHHCCCCEEEEEHHHCCCHHHHHCCCCCCCCCCCCCC
IHASDMILLPDPDTAKMDPFVAVPTLSLICNVFDPITKEDYTRDPRNIARKAEAYLKSTG
CCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
IGEIAFFGPEAEFFIFDEVRYDSSSNQSFYMVDSVEGAWNSGREEFPNLGYKPRHKEGYF
CCEEEEECCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCC
PLSPTDSQNDLRNEMVMELQKVGIRVECQHHEVATGGQAEIDMRFSSLVDMADQLQWFKY
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
VIKNVAYRNSKTVTFMPKPLYGDNGSGMHCHQSIWKNGQNLFAGDKYGGLSQMALWYIGG
HHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHH
IIKHAKALCAITNPTTNSYKRLVPGFEAPVNMAYSSRNRSASIRIPMLSTNPKAKRIEYR
HHHHHHHEEEECCCCCCHHHHHCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCCEEEEC
TPDPSCNGYLAFAAMLMAGLDGIENKIDPGQPLDKDIYGLSPEELKDIPSAPGTLEDALN
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHCCCCCCHHHHHHH
ALRDDHEFLLKGDVFTPDVIEKWIEYKMEAEVNPVRMRPVPLEFALYYDI
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECC
>Mature Secondary Structure 
TPQEVVSFAKENGALMVDFKFMDFVGTWQHISVPITEFSEDTFEEGQGFDGSSIRGWQP
CHHHHHHHHHCCCCEEEEEEHHHHHCCCCEEEEEHHHCCCHHHHHCCCCCCCCCCCCCC
IHASDMILLPDPDTAKMDPFVAVPTLSLICNVFDPITKEDYTRDPRNIARKAEAYLKSTG
CCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
IGEIAFFGPEAEFFIFDEVRYDSSSNQSFYMVDSVEGAWNSGREEFPNLGYKPRHKEGYF
CCEEEEECCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCC
PLSPTDSQNDLRNEMVMELQKVGIRVECQHHEVATGGQAEIDMRFSSLVDMADQLQWFKY
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
VIKNVAYRNSKTVTFMPKPLYGDNGSGMHCHQSIWKNGQNLFAGDKYGGLSQMALWYIGG
HHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHH
IIKHAKALCAITNPTTNSYKRLVPGFEAPVNMAYSSRNRSASIRIPMLSTNPKAKRIEYR
HHHHHHHEEEECCCCCCHHHHHCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCCEEEEC
TPDPSCNGYLAFAAMLMAGLDGIENKIDPGQPLDKDIYGLSPEELKDIPSAPGTLEDALN
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHCCCCCCHHHHHHH
ALRDDHEFLLKGDVFTPDVIEKWIEYKMEAEVNPVRMRPVPLEFALYYDI
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1362348 [H]