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The map label for this gene is fcl [H]

Identifier: 78222526

GI number: 78222526

Start: 1474121

End: 1475086

Strand: Direct

Name: fcl [H]

Synonym: Gmet_1312

Alternate gene names: 78222526

Gene position: 1474121-1475086 (Clockwise)

Preceding gene: 78222525

Following gene: 78222527

Centisome position: 36.88

GC content: 55.28

Gene sequence:

>966_bases
ATGAATAAGGATTCGCGAATCTTCGTTGCCGGGTCCCATGGGCTCGTGGGTTCCGCACTTGTCAGGCAATTGCGGTCCCG
TGGGTATGTCAATCTCTTGCTTCCCGAGAAGAGGGAACTTGACCTGGCCCATCAGCAGGCCGTGGCGGACTTCTTTGCAA
GTCAAAAACCCGACTACGTATTCCTTGCTGCTGCCAAGGTCGGCGGCATCCATGCCAATAACACCTATCCTGCCGACTTC
ATCTACACCAACCTGATGATTCAGAACAACGTCATTCACCACGCCTGGCTGAACGGCGTCAAACGGCTCCTCTTCCTCGG
CTCATCCTGCATCTATCCGAAGCACGCACCGCAGCCCATGCAAGAGGAGCATCTCCTGACCGGTCCCCTGGAACCCACCA
ACGAGCCCTACGCCATAGCGAAAATTGCCGGCATCAAGATGTGCGAATCCTACAACCGACAATATGGCACCAAATTTGTA
GCAGTCATGCCTACCAATCTCTATGGTCCCGGCGACAACTTCCACCCCGAGAACAGCCATGTCCTCCCCGCGCTGATCCG
GCGGTTCCACGAAGCCAAGAGGCAGAACGCTTCATCGGTTGTTGTCTGGGGAAGCGGCACACCTCGCCGGGAGCTGCTCT
ATGTTGATGACATGGCGGAGGGGTGTCTTCATGTCATGGATTTAACGGATGATCAGCTTGCTTCGGAGCTGTTGAGCTAC
CCCAAGCCGTGTTTCGTGAATCTCGGCACGGGGCATGATGTCACCATCCGTGAACTGGCAGAAACAGTCCGTAATGTAGT
CGGGTTTTCCGGCGACCTGGTATTCGATGCATCAAAACCCGATGGAACTCCCCGCAAGCTTCAAGACATCTCACGGATGC
ACGGTCTAGGATGGCGGCACAGGGTGGAGCTTGAAGAGGGGATTCGGCGCACGTATCAGTGGTATGTGGAAAATTACGCA
AAGTGA

Upstream 100 bases:

>100_bases
AGTTTCAGGGAACTGGTGTCCGAGATGGTGCGCGAGGACCTGAGAGCCGCCGAACGTGATGAGTTGGTAAAGAAACACGG
CTACAAGGCGTTTGACCACC

Downstream 100 bases:

>100_bases
GGAAGTCGAACGTTGCGATCCGATCTTTCTGCATCTGTCGTAGATACCTTGAGGGCGGCGCTCGAGGATGAACCGCTGCT
AGAACTGGCAGTACTGATCG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNKDSRIFVAGSHGLVGSALVRQLRSRGYVNLLLPEKRELDLAHQQAVADFFASQKPDYVFLAAAKVGGIHANNTYPADF
IYTNLMIQNNVIHHAWLNGVKRLLFLGSSCIYPKHAPQPMQEEHLLTGPLEPTNEPYAIAKIAGIKMCESYNRQYGTKFV
AVMPTNLYGPGDNFHPENSHVLPALIRRFHEAKRQNASSVVVWGSGTPRRELLYVDDMAEGCLHVMDLTDDQLASELLSY
PKPCFVNLGTGHDVTIRELAETVRNVVGFSGDLVFDASKPDGTPRKLQDISRMHGLGWRHRVELEEGIRRTYQWYVENYA
K

Sequences:

>Translated_321_residues
MNKDSRIFVAGSHGLVGSALVRQLRSRGYVNLLLPEKRELDLAHQQAVADFFASQKPDYVFLAAAKVGGIHANNTYPADF
IYTNLMIQNNVIHHAWLNGVKRLLFLGSSCIYPKHAPQPMQEEHLLTGPLEPTNEPYAIAKIAGIKMCESYNRQYGTKFV
AVMPTNLYGPGDNFHPENSHVLPALIRRFHEAKRQNASSVVVWGSGTPRRELLYVDDMAEGCLHVMDLTDDQLASELLSY
PKPCFVNLGTGHDVTIRELAETVRNVVGFSGDLVFDASKPDGTPRKLQDISRMHGLGWRHRVELEEGIRRTYQWYVENYA
K
>Mature_321_residues
MNKDSRIFVAGSHGLVGSALVRQLRSRGYVNLLLPEKRELDLAHQQAVADFFASQKPDYVFLAAAKVGGIHANNTYPADF
IYTNLMIQNNVIHHAWLNGVKRLLFLGSSCIYPKHAPQPMQEEHLLTGPLEPTNEPYAIAKIAGIKMCESYNRQYGTKFV
AVMPTNLYGPGDNFHPENSHVLPALIRRFHEAKRQNASSVVVWGSGTPRRELLYVDDMAEGCLHVMDLTDDQLASELLSY
PKPCFVNLGTGHDVTIRELAETVRNVVGFSGDLVFDASKPDGTPRKLQDISRMHGLGWRHRVELEEGIRRTYQWYVENYA
K

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=320, Percent_Identity=31.25, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI7657641, Length=291, Percent_Identity=26.8041237113402, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI42516563, Length=327, Percent_Identity=25.0764525993884, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788365, Length=313, Percent_Identity=64.5367412140575, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI48994969, Length=361, Percent_Identity=26.0387811634349, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=317, Percent_Identity=29.9684542586751, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI19922778, Length=323, Percent_Identity=31.5789473684211, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI21356223, Length=334, Percent_Identity=27.2455089820359, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 36130; Mature: 36130

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDSRIFVAGSHGLVGSALVRQLRSRGYVNLLLPEKRELDLAHQQAVADFFASQKPDYV
CCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEE
FLAAAKVGGIHANNTYPADFIYTNLMIQNNVIHHAWLNGVKRLLFLGSSCIYPKHAPQPM
EEEEEEECCEECCCCCCHHHHEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
QEEHLLTGPLEPTNEPYAIAKIAGIKMCESYNRQYGTKFVAVMPTNLYGPGDNFHPENSH
CCCCCEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCC
VLPALIRRFHEAKRQNASSVVVWGSGTPRRELLYVDDMAEGCLHVMDLTDDQLASELLSY
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHC
PKPCFVNLGTGHDVTIRELAETVRNVVGFSGDLVFDASKPDGTPRKLQDISRMHGLGWRH
CCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCE
RVELEEGIRRTYQWYVENYAK
EECHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKDSRIFVAGSHGLVGSALVRQLRSRGYVNLLLPEKRELDLAHQQAVADFFASQKPDYV
CCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEE
FLAAAKVGGIHANNTYPADFIYTNLMIQNNVIHHAWLNGVKRLLFLGSSCIYPKHAPQPM
EEEEEEECCEECCCCCCHHHHEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
QEEHLLTGPLEPTNEPYAIAKIAGIKMCESYNRQYGTKFVAVMPTNLYGPGDNFHPENSH
CCCCCEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCC
VLPALIRRFHEAKRQNASSVVVWGSGTPRRELLYVDDMAEGCLHVMDLTDDQLASELLSY
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHC
PKPCFVNLGTGHDVTIRELAETVRNVVGFSGDLVFDASKPDGTPRKLQDISRMHGLGWRH
CCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCE
RVELEEGIRRTYQWYVENYAK
EECHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]