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The map label for this gene is gmd [H]

Identifier: 78222525

GI number: 78222525

Start: 1473046

End: 1474128

Strand: Direct

Name: gmd [H]

Synonym: Gmet_1311

Alternate gene names: 78222525

Gene position: 1473046-1474128 (Clockwise)

Preceding gene: 78222524

Following gene: 78222526

Centisome position: 36.85

GC content: 56.69

Gene sequence:

>1083_bases
ATGAAAAAAGCTCTCATTACCGGTATTACCGGACAGGATGGATCGTACCTCGCAGAACTGTTGCTGGAAAAAGGTTACGA
GGTCCACGGCGTCAAGCGCAGGGCCTCTCTGTTTAACACGGATCGGATTGACCACCTCTACCAGGACCCCCACGAAACAG
GGCGGCGTTTCGTGCTTCACTATGGCGACATGACCGATTCCACCAACCTGATCAGAATCATCCAGTCGGTCCAACCCGAC
GAGATATACAATCTGGCAGCACAATCCCACGTGGCGGTATCGTTTGAAACGCCGGAATACACCGCCAATGCCGATGCCAT
CGGCCCCCTGCGGATCCTCGAAGCGATTCGCATCCTCGGCCTGGAAAAGAAGACCCGCTTTTACCAGGCATCAACGTCCG
AACTGTACGGTCTGGTTCAGGAAATCCCCCAGAAAGAGACGACCCCCTTCTACCCCCGCTCTCCCTATGCCGTTGCCAAG
CTCTACGCCTACTGGATCACCGTCAACTACCGCGAATCATACGGTATCTATGGCTGCAACGGCATCCTGTTCAATCACGA
ATCACCGGTGAGGGGGGAGACCTTCGTCACCCGCAAGATTACCCGCGCCTTGGCTCGCATCAAGCTGGGATTGCAGGAGT
GCCTCTATCTGGGCAACCTTGACGCAAAGCGCGACTGGGGGCATGCCCGGGATTATGTTGAAATGCAGTGGCTCCTGATG
CAGCAGGATGAGCCTGAAGACTGGGTCATTGCCACCGGTATCCAGTACAGCGTCCGTGACTTTGTCAACGCCGCTGCCGA
AGAGTTGGGGATGAAGATAGCGTGGCAAGGGGCGGGAGTCAATGAGACCGGAACCGATCACTCCGGCAAGGTCATGGTCA
AGGTCGATCCGCGCTATTTCCGCCCCACCGAGGTGGAGACCCTCCTGGGCGACGCGGCCAAGGCCAGGGAGAAACTGGGG
TGGGCTCCAAAAATCAGTTTCAGGGAACTGGTGTCCGAGATGGTGCGCGAGGACCTGAGAGCCGCCGAACGTGATGAGTT
GGTAAAGAAACACGGCTACAAGGCGTTTGACCACCATGAATAA

Upstream 100 bases:

>100_bases
CATCTTCACGGGCTTCCCTCAATGGCTGCCATGGAGAAGGCGAGGGCGTTGTTTATCGATTGACTTGAATATGAAAAATT
CAATGGAGATTGGTTACGGA

Downstream 100 bases:

>100_bases
GGATTCGCGAATCTTCGTTGCCGGGTCCCATGGGCTCGTGGGTTCCGCACTTGTCAGGCAATTGCGGTCCCGTGGGTATG
TCAATCTCTTGCTTCCCGAG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKKALITGITGQDGSYLAELLLEKGYEVHGVKRRASLFNTDRIDHLYQDPHETGRRFVLHYGDMTDSTNLIRIIQSVQPD
EIYNLAAQSHVAVSFETPEYTANADAIGPLRILEAIRILGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAK
LYAYWITVNYRESYGIYGCNGILFNHESPVRGETFVTRKITRALARIKLGLQECLYLGNLDAKRDWGHARDYVEMQWLLM
QQDEPEDWVIATGIQYSVRDFVNAAAEELGMKIAWQGAGVNETGTDHSGKVMVKVDPRYFRPTEVETLLGDAAKAREKLG
WAPKISFRELVSEMVREDLRAAERDELVKKHGYKAFDHHE

Sequences:

>Translated_360_residues
MKKALITGITGQDGSYLAELLLEKGYEVHGVKRRASLFNTDRIDHLYQDPHETGRRFVLHYGDMTDSTNLIRIIQSVQPD
EIYNLAAQSHVAVSFETPEYTANADAIGPLRILEAIRILGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAK
LYAYWITVNYRESYGIYGCNGILFNHESPVRGETFVTRKITRALARIKLGLQECLYLGNLDAKRDWGHARDYVEMQWLLM
QQDEPEDWVIATGIQYSVRDFVNAAAEELGMKIAWQGAGVNETGTDHSGKVMVKVDPRYFRPTEVETLLGDAAKAREKLG
WAPKISFRELVSEMVREDLRAAERDELVKKHGYKAFDHHE
>Mature_360_residues
MKKALITGITGQDGSYLAELLLEKGYEVHGVKRRASLFNTDRIDHLYQDPHETGRRFVLHYGDMTDSTNLIRIIQSVQPD
EIYNLAAQSHVAVSFETPEYTANADAIGPLRILEAIRILGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAK
LYAYWITVNYRESYGIYGCNGILFNHESPVRGETFVTRKITRALARIKLGLQECLYLGNLDAKRDWGHARDYVEMQWLLM
QQDEPEDWVIATGIQYSVRDFVNAAAEELGMKIAWQGAGVNETGTDHSGKVMVKVDPRYFRPTEVETLLGDAAKAREKLG
WAPKISFRELVSEMVREDLRAAERDELVKKHGYKAFDHHE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=339, Percent_Identity=63.716814159292, Blast_Score=454, Evalue=1e-128,
Organism=Escherichia coli, GI1788366, Length=363, Percent_Identity=72.1763085399449, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI48994969, Length=346, Percent_Identity=26.0115606936416, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788353, Length=377, Percent_Identity=23.342175066313, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=348, Percent_Identity=65.2298850574713, Blast_Score=479, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI133901790, Length=340, Percent_Identity=64.7058823529412, Blast_Score=473, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17539424, Length=340, Percent_Identity=64.7058823529412, Blast_Score=473, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI133901788, Length=340, Percent_Identity=64.7058823529412, Blast_Score=472, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17539422, Length=340, Percent_Identity=64.7058823529412, Blast_Score=472, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI133901786, Length=340, Percent_Identity=64.7058823529412, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24158427, Length=348, Percent_Identity=62.0689655172414, Blast_Score=461, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 41128; Mature: 41128

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALITGITGQDGSYLAELLLEKGYEVHGVKRRASLFNTDRIDHLYQDPHETGRRFVLH
CCCCEEEECCCCCHHHHHHHHHHCCCCEECHHHHHHHHCHHHHHHHHCCHHHHCCEEEEE
YGDMTDSTNLIRIIQSVQPDEIYNLAAQSHVAVSFETPEYTANADAIGPLRILEAIRILG
ECCCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHC
LEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGIYGCN
CCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEEECCCCEEECC
GILFNHESPVRGETFVTRKITRALARIKLGLQECLYLGNLDAKRDWGHARDYVEMQWLLM
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEE
QQDEPEDWVIATGIQYSVRDFVNAAAEELGMKIAWQGAGVNETGTDHSGKVMVKVDPRYF
CCCCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCC
RPTEVETLLGDAAKAREKLGWAPKISFRELVSEMVREDLRAAERDELVKKHGYKAFDHHE
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKALITGITGQDGSYLAELLLEKGYEVHGVKRRASLFNTDRIDHLYQDPHETGRRFVLH
CCCCEEEECCCCCHHHHHHHHHHCCCCEECHHHHHHHHCHHHHHHHHCCHHHHCCEEEEE
YGDMTDSTNLIRIIQSVQPDEIYNLAAQSHVAVSFETPEYTANADAIGPLRILEAIRILG
ECCCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHC
LEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGIYGCN
CCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEEECCCCEEECC
GILFNHESPVRGETFVTRKITRALARIKLGLQECLYLGNLDAKRDWGHARDYVEMQWLLM
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEE
QQDEPEDWVIATGIQYSVRDFVNAAAEELGMKIAWQGAGVNETGTDHSGKVMVKVDPRYF
CCCCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCC
RPTEVETLLGDAAKAREKLGWAPKISFRELVSEMVREDLRAAERDELVKKHGYKAFDHHE
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]