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The map label for this gene is ksgA

Identifier: 78222518

GI number: 78222518

Start: 1466845

End: 1467672

Strand: Direct

Name: ksgA

Synonym: Gmet_1304

Alternate gene names: 78222518

Gene position: 1466845-1467672 (Clockwise)

Preceding gene: 78222517

Following gene: 78222520

Centisome position: 36.69

GC content: 61.11

Gene sequence:

>828_bases
GTGAGAGGGGAGGGGATTCGCGCCCGCAAGGCGCTGGGGCAGAATTTTCTCGTGGACCGTCATGTTCTCGCACGGATCGC
GGACATCGTCGATATGCGGCCCGACGACCGCATTCTGGAAGTGGGGCCGGGAAAGGGCGCCCTCACCGAGATGCTGGCTC
ATCGATGCGCGCGCCTTGTGGCGGTGGAGTTGGACACGCGGCTCGTGCCGGTACTCCGCCAGGCCTTTCGGGATAACCCG
CGAGTGGAGATCGTCCACGGCGACATTCTGGAGATCGACCTGCGCGACCTCCTGGCTTATGAAGGGGGAGAACGGTGGAA
GGTGGCAGCCAATCTCCCCTACAACATATCCACGCCGGTTCTCTTTGCGTTTCTCGACAACCGCGATCTCTTCTCCCGTC
TGGTGCTCATGCTTCAGAAGGAAGTGGGCGACCGCCTCGCCGCCTCACCGGGGACTAAGGATTACGGCATCCTGTCGGTC
TTTTTCCAACTTCATTTTGATGTGACGCGGGAAATGATCGTCAGACCAGGTTCGTTCCATCCCGTGCCGAAGGTGGACTC
GGCCGTCCTGAGCTTCGTCCCCCTCGAAAAGCCCCGGGTGGCTGTGGGAGACGAGCAGTATTTCCGCCGCCTCGTGAAAG
GGGCTTTCTCCATGCGGCGAAAAACCCTCTGGAACTGTCTGAAAAATGCCGGCCTGGGGCTTTCCGGGGAGCAGTTGTCT
GAAGCCCTTGCAGTGTGCGGGATAGAGCCGGGGAGGCGTGGTGAGACCCTGAGCCTTGACGAGTTTGCGGCCCTTTCCCG
GGCCATGATGGCATTGGGGGGAAAATAG

Upstream 100 bases:

>100_bases
TTCCCGGTGATTTCTACCTCTCCCGGGGGATCAGCGACGGCCTCTCCCTGGACGCCTTGGTAAACTGGCCCTTGGATACG
ATCCGGACGAGGCTGGAGTC

Downstream 100 bases:

>100_bases
AAAAGCCGCCCGGAGAGGACGGCTTTCACCGTTGATGAGGGGAAAATGGCGGGTTAAGCCTTTTTCTTCCGCGGCGCTCC
CTTTGGCTTTGCCGGCTTCT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRGEGIRARKALGQNFLVDRHVLARIADIVDMRPDDRILEVGPGKGALTEMLAHRCARLVAVELDTRLVPVLRQAFRDNP
RVEIVHGDILEIDLRDLLAYEGGERWKVAANLPYNISTPVLFAFLDNRDLFSRLVLMLQKEVGDRLAASPGTKDYGILSV
FFQLHFDVTREMIVRPGSFHPVPKVDSAVLSFVPLEKPRVAVGDEQYFRRLVKGAFSMRRKTLWNCLKNAGLGLSGEQLS
EALAVCGIEPGRRGETLSLDEFAALSRAMMALGGK

Sequences:

>Translated_275_residues
MRGEGIRARKALGQNFLVDRHVLARIADIVDMRPDDRILEVGPGKGALTEMLAHRCARLVAVELDTRLVPVLRQAFRDNP
RVEIVHGDILEIDLRDLLAYEGGERWKVAANLPYNISTPVLFAFLDNRDLFSRLVLMLQKEVGDRLAASPGTKDYGILSV
FFQLHFDVTREMIVRPGSFHPVPKVDSAVLSFVPLEKPRVAVGDEQYFRRLVKGAFSMRRKTLWNCLKNAGLGLSGEQLS
EALAVCGIEPGRRGETLSLDEFAALSRAMMALGGK
>Mature_275_residues
MRGEGIRARKALGQNFLVDRHVLARIADIVDMRPDDRILEVGPGKGALTEMLAHRCARLVAVELDTRLVPVLRQAFRDNP
RVEIVHGDILEIDLRDLLAYEGGERWKVAANLPYNISTPVLFAFLDNRDLFSRLVLMLQKEVGDRLAASPGTKDYGILSV
FFQLHFDVTREMIVRPGSFHPVPKVDSAVLSFVPLEKPRVAVGDEQYFRRLVKGAFSMRRKTLWNCLKNAGLGLSGEQLS
EALAVCGIEPGRRGETLSLDEFAALSRAMMALGGK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=231, Percent_Identity=34.6320346320346, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI156415992, Length=285, Percent_Identity=32.6315789473684, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1786236, Length=266, Percent_Identity=36.0902255639098, Blast_Score=157, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI25146882, Length=193, Percent_Identity=35.2331606217617, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=286, Percent_Identity=28.6713286713287, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=217, Percent_Identity=32.7188940092166, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21358017, Length=233, Percent_Identity=34.7639484978541, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21357273, Length=252, Percent_Identity=31.3492063492063, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_GEOMG (Q39W34)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384265.1
- ProteinModelPortal:   Q39W34
- SMR:   Q39W34
- STRING:   Q39W34
- GeneID:   3739473
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1304
- NMPDR:   fig|269799.3.peg.827
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   Q39W34
- ProtClustDB:   PRK00274
- BioCyc:   GMET269799:GMET_1304-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30633; Mature: 30633

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS01131 RRNA_A_DIMETH; PS00307 LECTIN_LEGUME_BETA

Important sites: BINDING 15-15 BINDING 17-17 BINDING 42-42 BINDING 63-63 BINDING 88-88 BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGEGIRARKALGQNFLVDRHVLARIADIVDMRPDDRILEVGPGKGALTEMLAHRCARLV
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHH
AVELDTRLVPVLRQAFRDNPRVEIVHGDILEIDLRDLLAYEGGERWKVAANLPYNISTPV
HHHHHHHHHHHHHHHHCCCCCEEEEECCEEEECHHHHHHHCCCCEEEEEECCCCCCCCCE
LFAFLDNRDLFSRLVLMLQKEVGDRLAASPGTKDYGILSVFFQLHFDVTREMIVRPGSFH
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PVPKVDSAVLSFVPLEKPRVAVGDEQYFRRLVKGAFSMRRKTLWNCLKNAGLGLSGEQLS
CCCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
EALAVCGIEPGRRGETLSLDEFAALSRAMMALGGK
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRGEGIRARKALGQNFLVDRHVLARIADIVDMRPDDRILEVGPGKGALTEMLAHRCARLV
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHH
AVELDTRLVPVLRQAFRDNPRVEIVHGDILEIDLRDLLAYEGGERWKVAANLPYNISTPV
HHHHHHHHHHHHHHHHCCCCCEEEEECCEEEECHHHHHHHCCCCEEEEEECCCCCCCCCE
LFAFLDNRDLFSRLVLMLQKEVGDRLAASPGTKDYGILSVFFQLHFDVTREMIVRPGSFH
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PVPKVDSAVLSFVPLEKPRVAVGDEQYFRRLVKGAFSMRRKTLWNCLKNAGLGLSGEQLS
CCCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
EALAVCGIEPGRRGETLSLDEFAALSRAMMALGGK
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA