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The map label for this gene is btuB [H]

Identifier: 78222459

GI number: 78222459

Start: 1403629

End: 1405539

Strand: Direct

Name: btuB [H]

Synonym: Gmet_1245

Alternate gene names: 78222459

Gene position: 1403629-1405539 (Clockwise)

Preceding gene: 78222456

Following gene: 78222460

Centisome position: 35.11

GC content: 56.99

Gene sequence:

>1911_bases
ATGTCGTTTCAGGATAAAGCGATCAAGAAACGGATTTCTGTGGCCACGATAGCCTGTGCAGGGGCTGTGCTGTGGCTCCA
TGGCTCGGTTGTCCCGGCCTGGGCCGAAAACGACGAGATGAAGGTCCTTGAGATGTTTTACGAGGACAAAGAACTTGTGG
TCACTCCCACGCGGGATCCGAAGCCCATCTCCCAGGTGGCGGAAAACGTCACCGTCATCACCGCCAAGGAGATTCAGGCC
CTCAATGCCCACACCCTGGGAGAGGTTCTCAATACCATACCGGGGGTCCATGTGGAGCCCCGCGTGACGCCGGGAGGGTT
ATCCCCCACACTTTCCATCCAGGGGTCCGAGAGCAACCATGTCCGGGTGATCATGGATGGAGTGACCATCAACAACGTTT
CCGACTTCCTGGCGGATGTGGGGGCCATCCCTGTACAGCGGATCGCGGCGGTGGAGATAATCAAAGGGCCGGCGTCCTCG
GCATGGGGTTCTTCACTGGGGGGGATCATCAACATCATCACCAAGTCTCCGGACGAGGGACGGAAACTGGGCGGAACGGT
CTCGGCCTCCATGGGTGAGCGTACCACCGGCGACTTCCGCCTGGAACTCTCTGGCACCCGTTCTTCTCTTGGATATTACC
TGGCTGCCGACGGCATAACCTCCGACGGTCTCCTTCCCCATACGGGCGTGGACAGCGGCAGCGTATACGGCAAGATGACA
TGGGACCCCCAGGAGGATACCCGCGTGTTTGCCACGTATAGTTATTCCCGCGGGAAAAGGGGGTTGGGGGAGTTTCCCCA
GTATGGATTGCGTCAGGAGGGGGACTATGAGCATTTGATAGCAACCCTCGGCATGAGCCATGCCTTGAGTGAAAGCCTTG
ATCTGGATATCGCGGGCCGCTTCTCGCACCGCGATTACAGTGACGTTTCCGGTGCCTTGGGCGCGGGAGAGGGATTGAAC
AACAACAGCTATGAGCAGCCCAACCTCGGCGCAAGCGCCAAGCTCGCCTGGCGCACGGGCATTCACAGCGTGATAATTGG
AACGGATTACGACAACGGTACCATAGACGCAGATTTAAGGGAAAATGGCGGCACCGTTCGCACGAAGACCCATGGCCGCC
TGGAGCGGCGGGCGATTTTCGCCAACGACACAATCGTCTGGAACAACTGGTCGATCACCCCGGGTCTGCGTTACGACTGG
ACCAGCACCAGCGATGATTTCTTCAGCCCGAGCCTAGGGGTGACATATAACCTGAGTGACCACACGGTCCTGCGGGCTTA
TGTGGCCCGGGGCTTTAACGTCCCTTCTTTGACCGCGACTACCGTCGGCGCGCAGGGATTGCAGCCTAACCCTCATCTGG
AAATGGAGAAGGTCTGGTCCTATCAGCTTGGCTTCGAGACGACCTTTCTCAGGTATCTCTGGCTGAAGAATACGGTGTTC
CGTCATGATGTCTCTGACTTCCTTGCTTATGATGGTGCGACCAACAGTTATGTCAATCAGGGGAAGCAGCGTCGGCAGGG
GGTAGAGGTGGAGGTGAAATCCATCCCCTTTTTCAACACCACCATCGGCGTCGGCTATACATTCATCGATGCCCGTGATC
GGCTTGCCGATGAACGCGTGCCGAACATCCCTCGGCAAACGTGGGACGTATCCATAAATTATGACGACCGGAAACTCCTG
CGGGGAGGGCTTGTTGGGCGCTATACCCAATGGAACGCCAGAGAGGATTTTAACAGCCGCGAAAACGCCATGATCTGGGA
CCTGACCGGAGGGGTCCGGTTTCTGACCGGGCAGGTATCCGGCGAGATATTCTTCGTTGCCCATAACCTTTTCAATGGTT
CACAATATCTCCATTACTTCTTTAGGAATCCTGGTCGCTGGTTCGAGGGAGGGCTTCGCTTTAACTTTTGA

Upstream 100 bases:

>100_bases
TCTTTTTGCATGATCCGGTTTCTTGAAAATGCATTAATGGTTGCAGCATCGTGACAGCGAATAACACTATCGGGAACTTC
TGTAGGGTTGGGGGAGATGG

Downstream 100 bases:

>100_bases
GTTGACGAAATTCATAAATTGAATATTATTAATTTTTTTCTTCTTGATTACTTGTGTGCAGAAAGTAGAATGACGTTCTA
TCGGTAAATATCTCACAGGA

Product: TonB-dependent receptor, plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MSFQDKAIKKRISVATIACAGAVLWLHGSVVPAWAENDEMKVLEMFYEDKELVVTPTRDPKPISQVAENVTVITAKEIQA
LNAHTLGEVLNTIPGVHVEPRVTPGGLSPTLSIQGSESNHVRVIMDGVTINNVSDFLADVGAIPVQRIAAVEIIKGPASS
AWGSSLGGIINIITKSPDEGRKLGGTVSASMGERTTGDFRLELSGTRSSLGYYLAADGITSDGLLPHTGVDSGSVYGKMT
WDPQEDTRVFATYSYSRGKRGLGEFPQYGLRQEGDYEHLIATLGMSHALSESLDLDIAGRFSHRDYSDVSGALGAGEGLN
NNSYEQPNLGASAKLAWRTGIHSVIIGTDYDNGTIDADLRENGGTVRTKTHGRLERRAIFANDTIVWNNWSITPGLRYDW
TSTSDDFFSPSLGVTYNLSDHTVLRAYVARGFNVPSLTATTVGAQGLQPNPHLEMEKVWSYQLGFETTFLRYLWLKNTVF
RHDVSDFLAYDGATNSYVNQGKQRRQGVEVEVKSIPFFNTTIGVGYTFIDARDRLADERVPNIPRQTWDVSINYDDRKLL
RGGLVGRYTQWNAREDFNSRENAMIWDLTGGVRFLTGQVSGEIFFVAHNLFNGSQYLHYFFRNPGRWFEGGLRFNF

Sequences:

>Translated_636_residues
MSFQDKAIKKRISVATIACAGAVLWLHGSVVPAWAENDEMKVLEMFYEDKELVVTPTRDPKPISQVAENVTVITAKEIQA
LNAHTLGEVLNTIPGVHVEPRVTPGGLSPTLSIQGSESNHVRVIMDGVTINNVSDFLADVGAIPVQRIAAVEIIKGPASS
AWGSSLGGIINIITKSPDEGRKLGGTVSASMGERTTGDFRLELSGTRSSLGYYLAADGITSDGLLPHTGVDSGSVYGKMT
WDPQEDTRVFATYSYSRGKRGLGEFPQYGLRQEGDYEHLIATLGMSHALSESLDLDIAGRFSHRDYSDVSGALGAGEGLN
NNSYEQPNLGASAKLAWRTGIHSVIIGTDYDNGTIDADLRENGGTVRTKTHGRLERRAIFANDTIVWNNWSITPGLRYDW
TSTSDDFFSPSLGVTYNLSDHTVLRAYVARGFNVPSLTATTVGAQGLQPNPHLEMEKVWSYQLGFETTFLRYLWLKNTVF
RHDVSDFLAYDGATNSYVNQGKQRRQGVEVEVKSIPFFNTTIGVGYTFIDARDRLADERVPNIPRQTWDVSINYDDRKLL
RGGLVGRYTQWNAREDFNSRENAMIWDLTGGVRFLTGQVSGEIFFVAHNLFNGSQYLHYFFRNPGRWFEGGLRFNF
>Mature_635_residues
SFQDKAIKKRISVATIACAGAVLWLHGSVVPAWAENDEMKVLEMFYEDKELVVTPTRDPKPISQVAENVTVITAKEIQAL
NAHTLGEVLNTIPGVHVEPRVTPGGLSPTLSIQGSESNHVRVIMDGVTINNVSDFLADVGAIPVQRIAAVEIIKGPASSA
WGSSLGGIINIITKSPDEGRKLGGTVSASMGERTTGDFRLELSGTRSSLGYYLAADGITSDGLLPHTGVDSGSVYGKMTW
DPQEDTRVFATYSYSRGKRGLGEFPQYGLRQEGDYEHLIATLGMSHALSESLDLDIAGRFSHRDYSDVSGALGAGEGLNN
NSYEQPNLGASAKLAWRTGIHSVIIGTDYDNGTIDADLRENGGTVRTKTHGRLERRAIFANDTIVWNNWSITPGLRYDWT
STSDDFFSPSLGVTYNLSDHTVLRAYVARGFNVPSLTATTVGAQGLQPNPHLEMEKVWSYQLGFETTFLRYLWLKNTVFR
HDVSDFLAYDGATNSYVNQGKQRRQGVEVEVKSIPFFNTTIGVGYTFIDARDRLADERVPNIPRQTWDVSINYDDRKLLR
GGLVGRYTQWNAREDFNSRENAMIWDLTGGVRFLTGQVSGEIFFVAHNLFNGSQYLHYFFRNPGRWFEGGLRFNF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=571, Percent_Identity=26.6199649737303, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790405, Length=135, Percent_Identity=36.2962962962963, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 70259; Mature: 70128

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQDKAIKKRISVATIACAGAVLWLHGSVVPAWAENDEMKVLEMFYEDKELVVTPTRDP
CCCCHHHHHHHHHHHHHHHHHEEEEEECCEEECCCCCCCHHHHHHHHCCCEEEEECCCCC
KPISQVAENVTVITAKEIQALNAHTLGEVLNTIPGVHVEPRVTPGGLSPTLSIQGSESNH
CHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCCC
VRVIMDGVTINNVSDFLADVGAIPVQRIAAVEIIKGPASSAWGSSLGGIINIITKSPDEG
EEEEECCEEECCHHHHHHHHCCCCHHHHHEEEEECCCCCCCCCCCCCCEEEEEECCCCCC
RKLGGTVSASMGERTTGDFRLELSGTRSSLGYYLAADGITSDGLLPHTGVDSGSVYGKMT
HHCCCEEECCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEE
WDPQEDTRVFATYSYSRGKRGLGEFPQYGLRQEGDYEHLIATLGMSHALSESLDLDIAGR
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
FSHRDYSDVSGALGAGEGLNNNSYEQPNLGASAKLAWRTGIHSVIIGTDYDNGTIDADLR
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCEEEEEEC
ENGGTVRTKTHGRLERRAIFANDTIVWNNWSITPGLRYDWTSTSDDFFSPSLGVTYNLSD
CCCCEEEEECCCCCEEEEEEECCEEEECCEEECCCCEECCCCCCCCCCCCCCCEEEECCC
HTVLRAYVARGFNVPSLTATTVGAQGLQPNPHLEMEKVWSYQLGFETTFLRYLWLKNTVF
CHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHEEEECCCHHHHHHHHHHHHHHH
RHDVSDFLAYDGATNSYVNQGKQRRQGVEVEVKSIPFFNTTIGVGYTFIDARDRLADERV
HHCHHHHHHCCCCCCHHHHCCHHHHCCCEEEEECCCEEECCCCCCEEEECCHHHHHHHCC
PNIPRQTWDVSINYDDRKLLRGGLVGRYTQWNAREDFNSRENAMIWDLTGGVRFLTGQVS
CCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECC
GEIFFVAHNLFNGSQYLHYFFRNPGRWFEGGLRFNF
CEEEEEEECCCCCCCEEEEEECCCCCEECCCEEECC
>Mature Secondary Structure 
SFQDKAIKKRISVATIACAGAVLWLHGSVVPAWAENDEMKVLEMFYEDKELVVTPTRDP
CCCHHHHHHHHHHHHHHHHHEEEEEECCEEECCCCCCCHHHHHHHHCCCEEEEECCCCC
KPISQVAENVTVITAKEIQALNAHTLGEVLNTIPGVHVEPRVTPGGLSPTLSIQGSESNH
CHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCCC
VRVIMDGVTINNVSDFLADVGAIPVQRIAAVEIIKGPASSAWGSSLGGIINIITKSPDEG
EEEEECCEEECCHHHHHHHHCCCCHHHHHEEEEECCCCCCCCCCCCCCEEEEEECCCCCC
RKLGGTVSASMGERTTGDFRLELSGTRSSLGYYLAADGITSDGLLPHTGVDSGSVYGKMT
HHCCCEEECCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEE
WDPQEDTRVFATYSYSRGKRGLGEFPQYGLRQEGDYEHLIATLGMSHALSESLDLDIAGR
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
FSHRDYSDVSGALGAGEGLNNNSYEQPNLGASAKLAWRTGIHSVIIGTDYDNGTIDADLR
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCEEEEEEC
ENGGTVRTKTHGRLERRAIFANDTIVWNNWSITPGLRYDWTSTSDDFFSPSLGVTYNLSD
CCCCEEEEECCCCCEEEEEEECCEEEECCEEECCCCEECCCCCCCCCCCCCCCEEEECCC
HTVLRAYVARGFNVPSLTATTVGAQGLQPNPHLEMEKVWSYQLGFETTFLRYLWLKNTVF
CHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHEEEECCCHHHHHHHHHHHHHHH
RHDVSDFLAYDGATNSYVNQGKQRRQGVEVEVKSIPFFNTTIGVGYTFIDARDRLADERV
HHCHHHHHHCCCCCCHHHHCCHHHHCCCEEEEECCCEEECCCCCCEEEECCHHHHHHHCC
PNIPRQTWDVSINYDDRKLLRGGLVGRYTQWNAREDFNSRENAMIWDLTGGVRFLTGQVS
CCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECC
GEIFFVAHNLFNGSQYLHYFFRNPGRWFEGGLRFNF
CEEEEEEECCCCCCCEEEEEECCCCCEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11598060 [H]