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The map label for this gene is 78222387

Identifier: 78222387

GI number: 78222387

Start: 1294135

End: 1295826

Strand: Direct

Name: 78222387

Synonym: Gmet_1171

Alternate gene names: NA

Gene position: 1294135-1295826 (Clockwise)

Preceding gene: 78222386

Following gene: 78222390

Centisome position: 32.37

GC content: 60.05

Gene sequence:

>1692_bases
ATGCGGATACTACTCGTCTCTAGGCCCCATCCAGAAGGGGCCCGTGACCCGTTCACCTCACTCCTGCCCACGGGGCTCGG
CTACCTCAACGCTGTTCTGAGAGAAAATGGTTTTACGTCCTCCCTTGCCAACCTCTCTACTGCTTCCTGGCGCCAGGTGG
AAGAACTGCTCCGACGGGAGCGTCCTGCCATCCTTGGTATCTCCGTTTTTACCCACAACCGTTTCGAATCCCTTCGACTT
GCCGCCTTGGCGAAGAAAGTCGACCCCACCTGTCTAACAATCTTTGGCGGTCCCCATGCAACCCACGCTTTCGAGTCTAT
CCTGGTTAAGTATCGGGAGGTGGACGGGGTGATCCTCGGTGAGGGGGAGGAGACTTTTCTCGACCTCGCACGGGCTGTCG
AGCGGGGCGATCGCAGTCTGACCGGCATAGTGGGACTGGCTTATCGCAAGGGCGCCAAGGTTCACTCCGTGAAGCGACCC
CCTGTTGCGGATCTCGACGTGCTCCCGCTGCCGGCACTCCACATGAATGATGCCTGGGGATGCGACGTGCGCCGCCAGTT
GGAATTCATCATCACCTCCCGCGGATGTCCCGCTGCCTGCCGCTTCTGTTCGTCGCCGCGTTTTTGGGGGAAGTCACTCC
GTTTCCGCTCGCCACGAGCCATGGTCGATGAGATCCGCGCCATTCGCGACCGCTACGGCCTTATCTATTTTTCTCTCCGT
GACGATACCTTTACTGTTCGCAAGGATCGGGTGATTGACTTTTGCCGGTTGCTTCTGGAGGAAAAGGTCCATATCCTCTG
GAACTGCCAGTCGCGGGTGAACGCCGTGGACGAGGAGATGCTCATCTGGATGCGTAGGGCCGGGTGCGAATGCGTCCAGT
ACGGCGTCGAGTCGGGGTCGGAGAAAATACTCGCGTATCTCGGTAAGCAGATCACCCCCGAGCAGGTCCGAAGGGCCGCT
AAGGCCACCCGGCGTGCCGGGATAAGCCTCTCCATTTACCTGATCTCCGGAGTGCCCGGGGAGACTGACGATGACCTGCA
GGCGACCCTTACGCTCATCAGCAACATCCTCCCCCATGATGGCCATGTGGCCCCCTTGGCATACTACCCCGGGACGGCTC
TCTTCGATGAAGCGGTGAGAGAGGGGCGGGTGGGCGCTGATCTCTTTGAAAGGGAGAGGGGAGCGGCCCTGTATGTGCGA
CGCGATCCGTTCGTTGCCCGCTCGATGGAGACGCTCTTGCGGGCATTGACCCGTGTCGAGAGGAAAGCCCGGTTTGGGCC
GGCCGACTTCACTGCCCAGCGCGAGTTTCTTGGCTACTGCCACGGGACGAATCTTGGGCAGGGCGAGTGGCTTGAAGCCA
AAGGAGATATAGGAAAGGCTGAGGCCCTGTATCGTGAGATCGTCGAGCGGCAGCCGGAAAATCCCTGGGGGTGGCTGGCC
CTTGGAGGTCTCCATGGTTCACGGGGAGATGTGACGCGGGCAGGAAAGGCTTTTGAGCGGGCTATCTCACTTGTGCCCGC
TCATGTTCCTGCTTACCTGGCACTTGGTGACCTGCGCCTTGAGGAGGGTGATCAAAGGGGAGCAAAGCAGCTATACGAGA
CGGCGAAAAGGCTCAATCCGCGGGATACCGATGTCGGGGAGCGTTTGCGTATGGTGGTGGCGGAGCGTGGTACGAAAAAA
GGCGACACCTGA

Upstream 100 bases:

>100_bases
GTGGCCGACCACAGTTGGGTTGATCCCGCGGATATTCTCCGCTTCGATCTCCTTCCCGCCGACTACCCTCTGGCGAAGAA
GATAGTTCACGAGTTCAGCG

Downstream 100 bases:

>100_bases
AGGATGTCGCCTTTTTTCGTTGCGGTTTTACCGGAAATTACTTGCCGGCAATCCGGACGCCAGGAGCGAAAATAATCTTT
TCGAAGGTCCGGTTGAGGGT

Product: radical SAM family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MRILLVSRPHPEGARDPFTSLLPTGLGYLNAVLRENGFTSSLANLSTASWRQVEELLRRERPAILGISVFTHNRFESLRL
AALAKKVDPTCLTIFGGPHATHAFESILVKYREVDGVILGEGEETFLDLARAVERGDRSLTGIVGLAYRKGAKVHSVKRP
PVADLDVLPLPALHMNDAWGCDVRRQLEFIITSRGCPAACRFCSSPRFWGKSLRFRSPRAMVDEIRAIRDRYGLIYFSLR
DDTFTVRKDRVIDFCRLLLEEKVHILWNCQSRVNAVDEEMLIWMRRAGCECVQYGVESGSEKILAYLGKQITPEQVRRAA
KATRRAGISLSIYLISGVPGETDDDLQATLTLISNILPHDGHVAPLAYYPGTALFDEAVREGRVGADLFERERGAALYVR
RDPFVARSMETLLRALTRVERKARFGPADFTAQREFLGYCHGTNLGQGEWLEAKGDIGKAEALYREIVERQPENPWGWLA
LGGLHGSRGDVTRAGKAFERAISLVPAHVPAYLALGDLRLEEGDQRGAKQLYETAKRLNPRDTDVGERLRMVVAERGTKK
GDT

Sequences:

>Translated_563_residues
MRILLVSRPHPEGARDPFTSLLPTGLGYLNAVLRENGFTSSLANLSTASWRQVEELLRRERPAILGISVFTHNRFESLRL
AALAKKVDPTCLTIFGGPHATHAFESILVKYREVDGVILGEGEETFLDLARAVERGDRSLTGIVGLAYRKGAKVHSVKRP
PVADLDVLPLPALHMNDAWGCDVRRQLEFIITSRGCPAACRFCSSPRFWGKSLRFRSPRAMVDEIRAIRDRYGLIYFSLR
DDTFTVRKDRVIDFCRLLLEEKVHILWNCQSRVNAVDEEMLIWMRRAGCECVQYGVESGSEKILAYLGKQITPEQVRRAA
KATRRAGISLSIYLISGVPGETDDDLQATLTLISNILPHDGHVAPLAYYPGTALFDEAVREGRVGADLFERERGAALYVR
RDPFVARSMETLLRALTRVERKARFGPADFTAQREFLGYCHGTNLGQGEWLEAKGDIGKAEALYREIVERQPENPWGWLA
LGGLHGSRGDVTRAGKAFERAISLVPAHVPAYLALGDLRLEEGDQRGAKQLYETAKRLNPRDTDVGERLRMVVAERGTKK
GDT
>Mature_563_residues
MRILLVSRPHPEGARDPFTSLLPTGLGYLNAVLRENGFTSSLANLSTASWRQVEELLRRERPAILGISVFTHNRFESLRL
AALAKKVDPTCLTIFGGPHATHAFESILVKYREVDGVILGEGEETFLDLARAVERGDRSLTGIVGLAYRKGAKVHSVKRP
PVADLDVLPLPALHMNDAWGCDVRRQLEFIITSRGCPAACRFCSSPRFWGKSLRFRSPRAMVDEIRAIRDRYGLIYFSLR
DDTFTVRKDRVIDFCRLLLEEKVHILWNCQSRVNAVDEEMLIWMRRAGCECVQYGVESGSEKILAYLGKQITPEQVRRAA
KATRRAGISLSIYLISGVPGETDDDLQATLTLISNILPHDGHVAPLAYYPGTALFDEAVREGRVGADLFERERGAALYVR
RDPFVARSMETLLRALTRVERKARFGPADFTAQREFLGYCHGTNLGQGEWLEAKGDIGKAEALYREIVERQPENPWGWLA
LGGLHGSRGDVTRAGKAFERAISLVPAHVPAYLALGDLRLEEGDQRGAKQLYETAKRLNPRDTDVGERLRMVVAERGTKK
GDT

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 62965; Mature: 62965

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS50005 TPR L=RR ; PS50293 TPR_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLVSRPHPEGARDPFTSLLPTGLGYLNAVLRENGFTSSLANLSTASWRQVEELLRRE
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHC
RPAILGISVFTHNRFESLRLAALAKKVDPTCLTIFGGPHATHAFESILVKYREVDGVILG
CCCEEEEEEHCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCEEEEC
EGEETFLDLARAVERGDRSLTGIVGLAYRKGAKVHSVKRPPVADLDVLPLPALHMNDAWG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
CDVRRQLEFIITSRGCPAACRFCSSPRFWGKSLRFRSPRAMVDEIRAIRDRYGLIYFSLR
CCHHHHHHHHEECCCCHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEC
DDTFTVRKDRVIDFCRLLLEEKVHILWNCQSRVNAVDEEMLIWMRRAGCECVQYGVESGS
CCEEEEHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCH
EKILAYLGKQITPEQVRRAAKATRRAGISLSIYLISGVPGETDDDLQATLTLISNILPHD
HHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
GHVAPLAYYPGTALFDEAVREGRVGADLFERERGAALYVRRDPFVARSMETLLRALTRVE
CCEEEEEECCCHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHH
RKARFGPADFTAQREFLGYCHGTNLGQGEWLEAKGDIGKAEALYREIVERQPENPWGWLA
HHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEE
LGGLHGSRGDVTRAGKAFERAISLVPAHVPAYLALGDLRLEEGDQRGAKQLYETAKRLNP
EECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCC
RDTDVGERLRMVVAERGTKKGDT
CCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRILLVSRPHPEGARDPFTSLLPTGLGYLNAVLRENGFTSSLANLSTASWRQVEELLRRE
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHC
RPAILGISVFTHNRFESLRLAALAKKVDPTCLTIFGGPHATHAFESILVKYREVDGVILG
CCCEEEEEEHCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCEEEEC
EGEETFLDLARAVERGDRSLTGIVGLAYRKGAKVHSVKRPPVADLDVLPLPALHMNDAWG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
CDVRRQLEFIITSRGCPAACRFCSSPRFWGKSLRFRSPRAMVDEIRAIRDRYGLIYFSLR
CCHHHHHHHHEECCCCHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEC
DDTFTVRKDRVIDFCRLLLEEKVHILWNCQSRVNAVDEEMLIWMRRAGCECVQYGVESGS
CCEEEEHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCH
EKILAYLGKQITPEQVRRAAKATRRAGISLSIYLISGVPGETDDDLQATLTLISNILPHD
HHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
GHVAPLAYYPGTALFDEAVREGRVGADLFERERGAALYVRRDPFVARSMETLLRALTRVE
CCEEEEEECCCHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHH
RKARFGPADFTAQREFLGYCHGTNLGQGEWLEAKGDIGKAEALYREIVERQPENPWGWLA
HHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEE
LGGLHGSRGDVTRAGKAFERAISLVPAHVPAYLALGDLRLEEGDQRGAKQLYETAKRLNP
EECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCC
RDTDVGERLRMVVAERGTKKGDT
CCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]