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The map label for this gene is def [H]

Identifier: 78188466

GI number: 78188466

Start: 569511

End: 570110

Strand: Reverse

Name: def [H]

Synonym: Cag_0488

Alternate gene names: 78188466

Gene position: 570110-569511 (Counterclockwise)

Preceding gene: 78188470

Following gene: 78188465

Centisome position: 22.17

GC content: 46.5

Gene sequence:

>600_bases
ATGCTTTTCGCCCGACGTTCAGCCCAACACCATATGATTTTACCTATTACGATTTATAGCCATGAAGTGCTTCGCCAAAA
AGCAAAACCACTGAAAGGCGTTGATAAAGAAATTGAAACGCTCATTGCAGCAATGTTTGAATCCATGCACAACGCTTCGG
GTATTGGCTTAGCGGCACCACAAGTTGGACGCTCACTACGTTTACTCGTGTTGGATGTTTCATGCTTAAAAAATTATAAG
GATGAAAAACCCATGGTAGTGATTAATCCACACATTCTATCGGTACGAGGAGCTTGTGCTATGGAAGAGGGGTGCCTGAG
TGTACCCGGTGTGCAAGGCGATGTGGTGCGCCCCTCAGCAATTACGATGAAGTACCGCAATGAGCGCTTTGAAGAGCTGA
CCGCTGAATTTTCGGGCATGGTTGCCCGAGTACTTCAGCACGAAATTGATCACCTTGATGGCAAGCTTTTTGTGGATAGA
ATGGATAAGCGTGATAAGCGCAAAATTCAGCATGAACTTAATGAACTGACCGCAGGGCACATTTCTGCCGACTATCCCGT
AGAGCTGCACCCAGCAACTACCGCAAGCCATGCAGAATAA

Upstream 100 bases:

>100_bases
GCACGTAGCGGGGTTTTTTGTTTCAGGAATATTTCTTCACAAATTATTAACTGCTCCCTTTCGTTAAAGAATGCTTCATG
CTGGTTATTAACAATTTAGA

Downstream 100 bases:

>100_bases
AACCATTGCTCAATTGATTACAAGAGGCAATGAATGATTGATGAATCGGCAGAGCAGCAGGATGAGTACTCTGCACATGA
AGAGAGGAACTATGCGAATT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MLFARRSAQHHMILPITIYSHEVLRQKAKPLKGVDKEIETLIAAMFESMHNASGIGLAAPQVGRSLRLLVLDVSCLKNYK
DEKPMVVINPHILSVRGACAMEEGCLSVPGVQGDVVRPSAITMKYRNERFEELTAEFSGMVARVLQHEIDHLDGKLFVDR
MDKRDKRKIQHELNELTAGHISADYPVELHPATTASHAE

Sequences:

>Translated_199_residues
MLFARRSAQHHMILPITIYSHEVLRQKAKPLKGVDKEIETLIAAMFESMHNASGIGLAAPQVGRSLRLLVLDVSCLKNYK
DEKPMVVINPHILSVRGACAMEEGCLSVPGVQGDVVRPSAITMKYRNERFEELTAEFSGMVARVLQHEIDHLDGKLFVDR
MDKRDKRKIQHELNELTAGHISADYPVELHPATTASHAE
>Mature_199_residues
MLFARRSAQHHMILPITIYSHEVLRQKAKPLKGVDKEIETLIAAMFESMHNASGIGLAAPQVGRSLRLLVLDVSCLKNYK
DEKPMVVINPHILSVRGACAMEEGCLSVPGVQGDVVRPSAITMKYRNERFEELTAEFSGMVARVLQHEIDHLDGKLFVDR
MDKRDKRKIQHELNELTAGHISADYPVELHPATTASHAE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=188, Percent_Identity=30.8510638297872, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=36.5853658536585, Blast_Score=105, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645728, Length=156, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24645726, Length=160, Percent_Identity=30, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22271; Mature: 22271

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFARRSAQHHMILPITIYSHEVLRQKAKPLKGVDKEIETLIAAMFESMHNASGIGLAAP
CCCCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECH
QVGRSLRLLVLDVSCLKNYKDEKPMVVINPHILSVRGACAMEEGCLSVPGVQGDVVRPSA
HHCCCEEHEEEEHHHHHCCCCCCCEEEECCCEEEECCCHHHCCCCCCCCCCCCCCCCCCC
ITMKYRNERFEELTAEFSGMVARVLQHEIDHLDGKLFVDRMDKRDKRKIQHELNELTAGH
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHCCC
ISADYPVELHPATTASHAE
CCCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MLFARRSAQHHMILPITIYSHEVLRQKAKPLKGVDKEIETLIAAMFESMHNASGIGLAAP
CCCCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECH
QVGRSLRLLVLDVSCLKNYKDEKPMVVINPHILSVRGACAMEEGCLSVPGVQGDVVRPSA
HHCCCEEHEEEEHHHHHCCCCCCCEEEECCCEEEECCCHHHCCCCCCCCCCCCCCCCCCC
ITMKYRNERFEELTAEFSGMVARVLQHEIDHLDGKLFVDRMDKRDKRKIQHELNELTAGH
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHCCC
ISADYPVELHPATTASHAE
CCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA