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The map label for this gene is thiC

Identifier: 78188360

GI number: 78188360

Start: 441231

End: 442826

Strand: Reverse

Name: thiC

Synonym: Cag_0382

Alternate gene names: 78188360

Gene position: 442826-441231 (Counterclockwise)

Preceding gene: 78188364

Following gene: 78188359

Centisome position: 17.22

GC content: 50.94

Gene sequence:

>1596_bases
ATGAGTAAAGCTCCATTATTTGAAAAAATAAGCGTTAAAGGTACCCTTTTCCCAATTGAGGTAAGCATGAAGCGCTTACA
CCTCAGCCACCCCTACACCTGCAACGGGCAGGAATTTTCCTCCCTCCCAATGTACGACACCAGCGGCTTGCATGGCGATT
GCCGCACGCAGGTTGATCCGCATGTGGGGTTGCCGCCCCTTCGCTCATCGTGGAATTTTCCGCGCACAGTGCAGCTTGGC
GTTGCCCAAACGCAAATGCACTACGCTCGCAAAGGAGTTATTACGCCCGAAATGGAGTATGTGGCAATCCGCGAAAATCA
GCAATTGGAAGAGTGGATTCGCTCCTTTAACCGTCATGGAAAAAAAGTTGAACCCATTACACCCGAATTTGTGCGGCAGG
AAATTGCTGCTGGACGTGCCATAATTCCAGCAAATCTTAAACATCCCGAACTTGAACCCATGATTATTGGGCGCCATTTT
CGGGTAAAAATTAACTCCAACATTGGCAACTCAGCCATGGGCTCTTCAATTGAGGAGGAGGTTGAAAAAGCGGTGTGGTC
GTGCCGTTGGGGTGCTGATACGGTAATGGATTTAAGCACGGGGGCAAACATTCACCAAACGCGCGAATGGATTTTACGCA
ACTCTCCCGTTCCCATTGGCACCGTGCCTGTTTATCAAGCGCTTGAAAAAGCGGGCGGCAAAGCTGAAAATTTAACGTGG
GAACTTTACCGCGACACCTTGATTGAACAAGCCGAGCAAGGGGTTGACTACTTCACCATCCACGCAGGCATTTTGCAGGA
ACACCTTCCCTACGCCGAGCGCCGCTTAACGGGCATTGTATCGCGCGGAGGTTCCATTATGGCAACATGGTGCCGCCACC
ACCAGCAGGAAAACTTCCTTTACACCCACTTTAACGACATTTGTGAGATTCTAAAATCATACGACATTGCCGTATCGTTA
GGCGATGCGCTCCGTCCTGGCTCTATTTTGGATGCTAATGACGAAGCACAATTTGGCGAGCTGAAAGTACTGGGCGAACT
CACCAAACAAGCATGGCAGCATGAGGTGCAGGTAATGATTGAAGGACCAGGGCATGTGCCTCTCAATTTGATTGAGGAGA
ACATGCAAAAAGAGCTTGAACTCTGCTACGAAGCACCGTTTTACACGCTCGGACCGCTTATTACCGATATTGCCGCAGGG
TACGACCACATCAATTCCGCAATTGGCGGCGCCCTGCTTGCCAGCCTTGGATGCTCCATGCTCTGCTACGTAACCCCAAA
AGAGCACCTTGGTTTGCCCGACCGCAACGATGTTCGCGAAGGGGTAATTGCCCACAAAGTAGCCGCACATGGTGCCGATA
TTGCACGAGGCAACCCAACAGCATGGCTTCGTGATACATTAATGAGCCAAGCTCGTTACTCCTTTGCGTGGGAAGATCAA
TTTAACCTCTCGCTTGACCCCGAAAAAACACGCGCCGTCCATTCGCAAAGCATAGCCGCCAGCGGTTACAGCGCCCCCAA
CCCCGACTTTTGCACCATGTGCGGTCCCGATTTTTGTTCCATGAAGCGCACCCAAAAGATGGGGAAAGGGAACTAA

Upstream 100 bases:

>100_bases
GTGCAAAGTTGTATAATCCCGCTAATTTAGCTATTGTGATTATGAGTTACTCCTTCCCCCTTCTCTGCTCTTCTTAATTT
TGACTTTCTGCACCAATACC

Downstream 100 bases:

>100_bases
AGAGAACCTCAGCCACCACATGCTATAAAACGAAAAGTCCCCTCTCAAGAGGGGATTTTGGTGTGTGTTGTTATATCATG
AGTTTCGATTACTTGGCTTT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MSKAPLFEKISVKGTLFPIEVSMKRLHLSHPYTCNGQEFSSLPMYDTSGLHGDCRTQVDPHVGLPPLRSSWNFPRTVQLG
VAQTQMHYARKGVITPEMEYVAIRENQQLEEWIRSFNRHGKKVEPITPEFVRQEIAAGRAIIPANLKHPELEPMIIGRHF
RVKINSNIGNSAMGSSIEEEVEKAVWSCRWGADTVMDLSTGANIHQTREWILRNSPVPIGTVPVYQALEKAGGKAENLTW
ELYRDTLIEQAEQGVDYFTIHAGILQEHLPYAERRLTGIVSRGGSIMATWCRHHQQENFLYTHFNDICEILKSYDIAVSL
GDALRPGSILDANDEAQFGELKVLGELTKQAWQHEVQVMIEGPGHVPLNLIEENMQKELELCYEAPFYTLGPLITDIAAG
YDHINSAIGGALLASLGCSMLCYVTPKEHLGLPDRNDVREGVIAHKVAAHGADIARGNPTAWLRDTLMSQARYSFAWEDQ
FNLSLDPEKTRAVHSQSIAASGYSAPNPDFCTMCGPDFCSMKRTQKMGKGN

Sequences:

>Translated_531_residues
MSKAPLFEKISVKGTLFPIEVSMKRLHLSHPYTCNGQEFSSLPMYDTSGLHGDCRTQVDPHVGLPPLRSSWNFPRTVQLG
VAQTQMHYARKGVITPEMEYVAIRENQQLEEWIRSFNRHGKKVEPITPEFVRQEIAAGRAIIPANLKHPELEPMIIGRHF
RVKINSNIGNSAMGSSIEEEVEKAVWSCRWGADTVMDLSTGANIHQTREWILRNSPVPIGTVPVYQALEKAGGKAENLTW
ELYRDTLIEQAEQGVDYFTIHAGILQEHLPYAERRLTGIVSRGGSIMATWCRHHQQENFLYTHFNDICEILKSYDIAVSL
GDALRPGSILDANDEAQFGELKVLGELTKQAWQHEVQVMIEGPGHVPLNLIEENMQKELELCYEAPFYTLGPLITDIAAG
YDHINSAIGGALLASLGCSMLCYVTPKEHLGLPDRNDVREGVIAHKVAAHGADIARGNPTAWLRDTLMSQARYSFAWEDQ
FNLSLDPEKTRAVHSQSIAASGYSAPNPDFCTMCGPDFCSMKRTQKMGKGN
>Mature_530_residues
SKAPLFEKISVKGTLFPIEVSMKRLHLSHPYTCNGQEFSSLPMYDTSGLHGDCRTQVDPHVGLPPLRSSWNFPRTVQLGV
AQTQMHYARKGVITPEMEYVAIRENQQLEEWIRSFNRHGKKVEPITPEFVRQEIAAGRAIIPANLKHPELEPMIIGRHFR
VKINSNIGNSAMGSSIEEEVEKAVWSCRWGADTVMDLSTGANIHQTREWILRNSPVPIGTVPVYQALEKAGGKAENLTWE
LYRDTLIEQAEQGVDYFTIHAGILQEHLPYAERRLTGIVSRGGSIMATWCRHHQQENFLYTHFNDICEILKSYDIAVSLG
DALRPGSILDANDEAQFGELKVLGELTKQAWQHEVQVMIEGPGHVPLNLIEENMQKELELCYEAPFYTLGPLITDIAAGY
DHINSAIGGALLASLGCSMLCYVTPKEHLGLPDRNDVREGVIAHKVAAHGADIARGNPTAWLRDTLMSQARYSFAWEDQF
NLSLDPEKTRAVHSQSIAASGYSAPNPDFCTMCGPDFCSMKRTQKMGKGN

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=573, Percent_Identity=57.2425828970332, Blast_Score=657, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_CHLCH (Q3ATM0)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_378698.1
- STRING:   Q3ATM0
- GeneID:   3747550
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0382
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   CCHL340177:CAG_0382-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 59333; Mature: 59202

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: BINDING 167-167 BINDING 196-196 BINDING 225-225 BINDING 261-261 BINDING 361-361 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAPLFEKISVKGTLFPIEVSMKRLHLSHPYTCNGQEFSSLPMYDTSGLHGDCRTQVDP
CCCCCCCCEEECCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HVGLPPLRSSWNFPRTVQLGVAQTQMHYARKGVITPEMEYVAIRENQQLEEWIRSFNRHG
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCC
KKVEPITPEFVRQEIAAGRAIIPANLKHPELEPMIIGRHFRVKINSNIGNSAMGSSIEEE
CCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEECCEEEEEECCCCCCCHHCCHHHHH
VEKAVWSCRWGADTVMDLSTGANIHQTREWILRNSPVPIGTVPVYQALEKAGGKAENLTW
HHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHH
ELYRDTLIEQAEQGVDYFTIHAGILQEHLPYAERRLTGIVSRGGSIMATWCRHHQQENFL
HHHHHHHHHHHHCCCCEEEEEHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEE
YTHFNDICEILKSYDIAVSLGDALRPGSILDANDEAQFGELKVLGELTKQAWQHEVQVMI
EHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEE
EGPGHVPLNLIEENMQKELELCYEAPFYTLGPLITDIAAGYDHINSAIGGALLASLGCSM
ECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LCYVTPKEHLGLPDRNDVREGVIAHKVAAHGADIARGNPTAWLRDTLMSQARYSFAWEDQ
EEEECCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEECCC
FNLSLDPEKTRAVHSQSIAASGYSAPNPDFCTMCGPDFCSMKRTQKMGKGN
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKAPLFEKISVKGTLFPIEVSMKRLHLSHPYTCNGQEFSSLPMYDTSGLHGDCRTQVDP
CCCCCCCEEECCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HVGLPPLRSSWNFPRTVQLGVAQTQMHYARKGVITPEMEYVAIRENQQLEEWIRSFNRHG
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCC
KKVEPITPEFVRQEIAAGRAIIPANLKHPELEPMIIGRHFRVKINSNIGNSAMGSSIEEE
CCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEECCEEEEEECCCCCCCHHCCHHHHH
VEKAVWSCRWGADTVMDLSTGANIHQTREWILRNSPVPIGTVPVYQALEKAGGKAENLTW
HHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHH
ELYRDTLIEQAEQGVDYFTIHAGILQEHLPYAERRLTGIVSRGGSIMATWCRHHQQENFL
HHHHHHHHHHHHCCCCEEEEEHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEE
YTHFNDICEILKSYDIAVSLGDALRPGSILDANDEAQFGELKVLGELTKQAWQHEVQVMI
EHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEE
EGPGHVPLNLIEENMQKELELCYEAPFYTLGPLITDIAAGYDHINSAIGGALLASLGCSM
ECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LCYVTPKEHLGLPDRNDVREGVIAHKVAAHGADIARGNPTAWLRDTLMSQARYSFAWEDQ
EEEECCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEECCC
FNLSLDPEKTRAVHSQSIAASGYSAPNPDFCTMCGPDFCSMKRTQKMGKGN
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA