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The map label for this gene is argT [H]

Identifier: 78067200

GI number: 78067200

Start: 2836667

End: 2837449

Strand: Direct

Name: argT [H]

Synonym: Bcep18194_A5731

Alternate gene names: 78067200

Gene position: 2836667-2837449 (Clockwise)

Preceding gene: 78067198

Following gene: 78067201

Centisome position: 76.79

GC content: 63.98

Gene sequence:

>783_bases
ATGAAGAAGGCCGCCCTGTGCGCCGCCCTCGCCCTCATGGCGGGTAGCGCCTTCGCGAAGGAGTGGAAGACCGTGCGGAT
CGGCGTCGATGCCAGCTACCCGCCGTTCGAGTCGACCGCGCCGAGCGGCGAGATCGTCGGTTTCGACGTCGATCTCACCA
AGGAAATCTGCAAGCGAATCAACGTGAATTGCGTGTGGGTGGCGCAGGATCTCGACGGGATCATCCCGGCGCTGAAGGCG
AAGAAGTACGACGTCATCGTGTCGTCGCTGACCGTCACGGACAAGCGCCGCGAACAGATCGACTTCTCCGACAAGGTATA
CGACGCGCCGGCGCGGATGATCGCGAAGACCGGCTCGCCGCTGCTGCCGACGATCGCATCGCTGAAGGGCAAGCGCGTGG
GCGTCGAGCAGGGCTCGACGCAGGAAACCTACGCGAAGGCGTACTGGGAGCCGCAGGGCGTGACGATCATTCCTTACCAG
AACCAGGACCAGGTCTATGCCGATCTCGGCTCGGGCCGTCTCGATGCGACGCTGCAGGACGAGCTGCAGGCCGACTACGG
CTTCCTGCGCACGCCGCGCGGCAAGGGCTTCGCGTTCGCCGGGCCGGAAGTGAAGGATCCGAAGACGATCGGCGACGGTA
CCGCAATCGGCCTGCGCAAGGAAGATACGGACCTGAAGCTCAAGATCAACAAGGCGCTGGCCGACATGCACAAGGACGGC
ACGTACGATCGGCTGTCGCACAAGTATTTCGCGTTCAGCGTCTATTCGGCTGCGGCCCGCTGA

Upstream 100 bases:

>100_bases
AGAGCGCCGATCGGCGATTTTTGGCCGTCTAGAATACGCCGCGGCGCCCGGTCCGACCGGCCGCGAGCACTCCCGGAAAG
TCAGCAACAGAAGGATGGAA

Downstream 100 bases:

>100_bases
GCGACGAAAAGAAGAGACAACGGACGCGGGGGCGGCCTCGCATCCGGGCAGTACAGGCAGTCAACCACGCGCCGCCCGCT
CCCTTGCCCGCCGCTCGCGC

Product: lysine/arginine/ornithine ABC transporter periplasmic ligand-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKKAALCAALALMAGSAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLTKEICKRINVNCVWVAQDLDGIIPALKA
KKYDVIVSSLTVTDKRREQIDFSDKVYDAPARMIAKTGSPLLPTIASLKGKRVGVEQGSTQETYAKAYWEPQGVTIIPYQ
NQDQVYADLGSGRLDATLQDELQADYGFLRTPRGKGFAFAGPEVKDPKTIGDGTAIGLRKEDTDLKLKINKALADMHKDG
TYDRLSHKYFAFSVYSAAAR

Sequences:

>Translated_260_residues
MKKAALCAALALMAGSAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLTKEICKRINVNCVWVAQDLDGIIPALKA
KKYDVIVSSLTVTDKRREQIDFSDKVYDAPARMIAKTGSPLLPTIASLKGKRVGVEQGSTQETYAKAYWEPQGVTIIPYQ
NQDQVYADLGSGRLDATLQDELQADYGFLRTPRGKGFAFAGPEVKDPKTIGDGTAIGLRKEDTDLKLKINKALADMHKDG
TYDRLSHKYFAFSVYSAAAR
>Mature_260_residues
MKKAALCAALALMAGSAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLTKEICKRINVNCVWVAQDLDGIIPALKA
KKYDVIVSSLTVTDKRREQIDFSDKVYDAPARMIAKTGSPLLPTIASLKGKRVGVEQGSTQETYAKAYWEPQGVTIIPYQ
NQDQVYADLGSGRLDATLQDELQADYGFLRTPRGKGFAFAGPEVKDPKTIGDGTAIGLRKEDTDLKLKINKALADMHKDG
TYDRLSHKYFAFSVYSAAAR

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=56.1538461538462, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=54.0772532188841, Blast_Score=282, Evalue=1e-77,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=37.0517928286853, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=34.4, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1788228, Length=263, Percent_Identity=31.5589353612167, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=31.8548387096774, Blast_Score=114, Evalue=7e-27,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28438; Mature: 28438

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAALCAALALMAGSAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLTKEICKRI
CCHHHHHHHHHHHHCHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHC
NVNCVWVAQDLDGIIPALKAKKYDVIVSSLTVTDKRREQIDFSDKVYDAPARMIAKTGSP
CCCEEEEECCCHHHHHHHCCCCEEEEEEEEEECCCHHHHCCCCCHHHHHHHHHHHHCCCC
LLPTIASLKGKRVGVEQGSTQETYAKAYWEPQGVTIIPYQNQDQVYADLGSGRLDATLQD
HHHHHHHCCCCEECCCCCCCHHHHHHHEECCCCEEEEEECCCCCEEEECCCCCCCCHHHH
ELQADYGFLRTPRGKGFAFAGPEVKDPKTIGDGTAIGLRKEDTDLKLKINKALADMHKDG
HHHHCCCEEECCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHCCC
TYDRLSHKYFAFSVYSAAAR
CHHHHCCEEEEEEEEHHHCC
>Mature Secondary Structure
MKKAALCAALALMAGSAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLTKEICKRI
CCHHHHHHHHHHHHCHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHC
NVNCVWVAQDLDGIIPALKAKKYDVIVSSLTVTDKRREQIDFSDKVYDAPARMIAKTGSP
CCCEEEEECCCHHHHHHHCCCCEEEEEEEEEECCCHHHHCCCCCHHHHHHHHHHHHCCCC
LLPTIASLKGKRVGVEQGSTQETYAKAYWEPQGVTIIPYQNQDQVYADLGSGRLDATLQD
HHHHHHHCCCCEECCCCCCCHHHHHHHEECCCCEEEEEECCCCCEEEECCCCCCCCHHHH
ELQADYGFLRTPRGKGFAFAGPEVKDPKTIGDGTAIGLRKEDTDLKLKINKALADMHKDG
HHHHCCCEEECCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHCCC
TYDRLSHKYFAFSVYSAAAR
CHHHHCCEEEEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]