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The map label for this gene is rotA [H]

Identifier: 78066855

GI number: 78066855

Start: 2452969

End: 2453550

Strand: Direct

Name: rotA [H]

Synonym: Bcep18194_A5386

Alternate gene names: 78066855

Gene position: 2452969-2453550 (Clockwise)

Preceding gene: 78066854

Following gene: 78066856

Centisome position: 66.4

GC content: 64.09

Gene sequence:

>582_bases
ATGAAACGTCTGTTGCTGGCGCTTGGCGGCGCCGCCCTTCTCGCGACCGCGACCGCACCTGCGTTCGCGCAATCGGCTAC
CGCGCACCCCGTCGTGCAGCTGAAGACCTCGCAGGGCGACATCCGCGTCGAGCTCTACCCGGAGAAGGCGCCGAAGTCCG
TCGCCAACTTCCTCGATTACGTGAAGGCCGGCCAGTACAACGGCACGATCTTCCATCGCGTGATCAAGGGCTTCATGATC
CAGGGCGGCGGCTACAAGACCAACTTCGAGGAGAAGCCGACCCGCGCGCCGATCCCGCTGGAGAGCCGCAATGGTCTGAA
GAACCTGACGGGCACGATCGCGATGGCACGCACGAGCGATCCGAACTCGGCCACCGCGCAGTTCTTCATCAACACGGTCG
ACAACAGCGGCCTCGACTACCCGAACCCGGACGGCAACGGCTATGCGGTGTTCGGCAAGGTCGTGTCGGGTCTCGACGTC
GTGAAGAAGATCGAAGGCGTCGCGACGACCTCGCGCGGCCCGATGCAAGACGTGCCCGCGCAACCGATCGTGATCGAATC
GGCCAGCATCGTCTCGAAGTAA

Upstream 100 bases:

>100_bases
GTTCCAGTTCAGCGGTCCGTCGGGCGCACTGGCGGCGCCGTACGTCGCGCCGTCGCAATAAGCGGCGCGGCCTTCTTTCC
CTCCCAACCTGAGGATCGCA

Downstream 100 bases:

>100_bases
GAACCTGCCGGCACGTCCGGCGCCACACGCGGCCGCGTCGCACGACCGGCCGCGTGCCATTTAAACCGCCTCACCGAAGG
AATTCATCATGGTTGAACTG

Product: peptidylprolyl isomerase

Products: NA

Alternate protein names: PPIase A; Cyclophilin A; Rotamase A [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MKRLLLALGGAALLATATAPAFAQSATAHPVVQLKTSQGDIRVELYPEKAPKSVANFLDYVKAGQYNGTIFHRVIKGFMI
QGGGYKTNFEEKPTRAPIPLESRNGLKNLTGTIAMARTSDPNSATAQFFINTVDNSGLDYPNPDGNGYAVFGKVVSGLDV
VKKIEGVATTSRGPMQDVPAQPIVIESASIVSK

Sequences:

>Translated_193_residues
MKRLLLALGGAALLATATAPAFAQSATAHPVVQLKTSQGDIRVELYPEKAPKSVANFLDYVKAGQYNGTIFHRVIKGFMI
QGGGYKTNFEEKPTRAPIPLESRNGLKNLTGTIAMARTSDPNSATAQFFINTVDNSGLDYPNPDGNGYAVFGKVVSGLDV
VKKIEGVATTSRGPMQDVPAQPIVIESASIVSK
>Mature_193_residues
MKRLLLALGGAALLATATAPAFAQSATAHPVVQLKTSQGDIRVELYPEKAPKSVANFLDYVKAGQYNGTIFHRVIKGFMI
QGGGYKTNFEEKPTRAPIPLESRNGLKNLTGTIAMARTSDPNSATAQFFINTVDNSGLDYPNPDGNGYAVFGKVVSGLDV
VKKIEGVATTSRGPMQDVPAQPIVIESASIVSK

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=170, Percent_Identity=34.1176470588235, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI22547212, Length=162, Percent_Identity=33.9506172839506, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI7657473, Length=162, Percent_Identity=33.9506172839506, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI22547215, Length=162, Percent_Identity=33.9506172839506, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI7706339, Length=162, Percent_Identity=35.8024691358025, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI24308049, Length=168, Percent_Identity=33.9285714285714, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI5454154, Length=185, Percent_Identity=34.0540540540541, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI4758950, Length=201, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=9e-13,
Organism=Homo sapiens, GI64276486, Length=135, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789763, Length=191, Percent_Identity=52.8795811518325, Blast_Score=185, Evalue=2e-48,
Organism=Escherichia coli, GI1786736, Length=162, Percent_Identity=54.320987654321, Blast_Score=184, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI71980594, Length=144, Percent_Identity=36.8055555555556, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71980590, Length=144, Percent_Identity=36.8055555555556, Blast_Score=90, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17539496, Length=154, Percent_Identity=32.4675324675325, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17532641, Length=165, Percent_Identity=32.7272727272727, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559074, Length=160, Percent_Identity=33.75, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17552780, Length=202, Percent_Identity=31.1881188118812, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17566266, Length=158, Percent_Identity=33.5443037974684, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17565860, Length=184, Percent_Identity=32.0652173913043, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17506307, Length=165, Percent_Identity=32.1212121212121, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323562, Length=159, Percent_Identity=36.4779874213836, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320359, Length=144, Percent_Identity=34.7222222222222, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320510, Length=200, Percent_Identity=33.5, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922376, Length=170, Percent_Identity=32.9411764705882, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17986117, Length=170, Percent_Identity=32.3529411764706, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922912, Length=175, Percent_Identity=34.2857142857143, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45549139, Length=140, Percent_Identity=38.5714285714286, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20130249, Length=154, Percent_Identity=36.3636363636364, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136360, Length=205, Percent_Identity=28.780487804878, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652460, Length=147, Percent_Identity=31.9727891156463, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 20453; Mature: 20453

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLLALGGAALLATATAPAFAQSATAHPVVQLKTSQGDIRVELYPEKAPKSVANFLDY
CCCEEEECCCHHHHHHHCCCHHHHCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHH
VKAGQYNGTIFHRVIKGFMIQGGGYKTNFEEKPTRAPIPLESRNGLKNLTGTIAMARTSD
HHCCCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECCC
PNSATAQFFINTVDNSGLDYPNPDGNGYAVFGKVVSGLDVVKKIEGVATTSRGPMQDVPA
CCCCEEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QPIVIESASIVSK
CCEEEECCHHHCC
>Mature Secondary Structure
MKRLLLALGGAALLATATAPAFAQSATAHPVVQLKTSQGDIRVELYPEKAPKSVANFLDY
CCCEEEECCCHHHHHHHCCCHHHHCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHH
VKAGQYNGTIFHRVIKGFMIQGGGYKTNFEEKPTRAPIPLESRNGLKNLTGTIAMARTSD
HHCCCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECCC
PNSATAQFFINTVDNSGLDYPNPDGNGYAVFGKVVSGLDVVKKIEGVATTSRGPMQDVPA
CCCCEEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QPIVIESASIVSK
CCEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9418240 [H]