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The map label for this gene is dnaB [H]

Identifier: 78066639

GI number: 78066639

Start: 2217788

End: 2219170

Strand: Reverse

Name: dnaB [H]

Synonym: Bcep18194_A5170

Alternate gene names: 78066639

Gene position: 2219170-2217788 (Counterclockwise)

Preceding gene: 78066640

Following gene: 78066638

Centisome position: 60.07

GC content: 64.35

Gene sequence:

>1383_bases
ATGAACGCGCCGCAAGATCCTCAAATCGAATCGCTGAAAGTCCCGCCGCATTCGGTCGAAGCCGAGCAGTCGGTGCTCGG
CGGCCTGTTGCTCGACAACGCGGCCTGGGACCGGATTGCCGACTTCCTGTCGCAGGGCGACTTCTACCGCTATGACCACC
GGATCATCTACGAGCACATCGGCCGCCTGATCGCGTCGACGCGCCCGGCCGACGTCGTGACCGTGTACGAAGCGCTGACC
ACGTCCGGCAAGGCCGACGACGTCGGCGGGCTCGCGTACCTGAATGCCCTCGCACAGAACACGCCGAGCGCGGCGAACAT
CCGCCGCTATGCGGAAATCGTGCGCGACCGCGCGGTGCTGCGCCGGCTCGTGTCGGTCGCCGACGAAATCTCGGCCGATG
CGTTCAACCCGCAAGGCAAGGAAGTCCGTCAGCTGCTCGACGAGGCCGAGTCGAAGGTGTTCTCGATCGCCGAGGATGGC
GCGCGCGGCAACCAGGGCTTCCTCGAGATCGGTCCGCTGCTCACGCAGGTCGTCGAGCGCATCGATACGCTGTACCACAC
GGCAAATCCGAGTGACGTCACGGGCACCCCGACGGGCTTCGTCGACCTCGACCGGATGACGTCCGGGATGCACGGTGGCG
AACTGATCATCGTCGCAGGGCGTCCGTCGATGGGTAAGACCGCGTTCTCGATGAACATCGGCGAATACGTGGCGGTCGAG
TACGGGCTGCCGGTCGCGGTGTTCTCGATGGAAATGCCGGGCACCCAGCTCGTGATGCGTATGCTCGGCTCGATCGGCCG
GCTCGACCAGCACCGGATGCGGACGGGGCGCCTGACGGACGAGGATTGGCCGAAGCTGACGCACGCGGTGCAGAAGATGA
GCGAGGCACAGCTCTTCATCGACGAAACCGGTGGCCTGAACCCGATGGAATTGCGCTCGCGCGCGCGGCGTCTTGCGCGG
CAATGCGGCAAGCTCGGCCTGATCATCGTCGACTACCTGCAGCTGATGTCGGGTTCGTCGCAGGGCGAGAACCGCGCGAC
CGAAATCTCGGAAATCTCGCGATCGCTGAAGAGTCTCGCGAAGGAACTGGACGTGCCGGTGATCGCGCTGTCGCAGCTGA
ACCGCGGCCTCGAGCAGCGTCCGAACAAGCGTCCGGTGATGTCGGATTTGCGTGAATCAGGCGCAATCGAACAGGACGCG
GACGTGATCCTGTTCATCTACCGCGACGAAGTCTACAACCCGGACAGCCCCGACAAGGGCACGGCCGAGATCATCATCGG
CAAGCAGCGTAACGGCCCGATCGGCCCCGTTCGCCTCACGTTCCTGGGGCAATACACGAAGTTCGACAACTTTGCAGGGG
CGCAGACGTTCTACGGCGAGTAA

Upstream 100 bases:

>100_bases
AAGGGCAGGCGCCCGGGCAACCGGGGCCTGCCTTTTTTATTGCCCGCTCGCTGGTGGCCGGTATGGCGCCGTCCTGTGTA
ATTCGCGATAATCCCAACCC

Downstream 100 bases:

>100_bases
CGCCGGTTTGTAAAGCCCTAAATCGCCAAACGTTGTATCCAATCCCGGCGCGCGCATGTGCGCGCGTCGCGGCACGACCG
AATAACGGTACAATGTCGCC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNAPQDPQIESLKVPPHSVEAEQSVLGGLLLDNAAWDRIADFLSQGDFYRYDHRIIYEHIGRLIASTRPADVVTVYEALT
TSGKADDVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVLRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDG
ARGNQGFLEIGPLLTQVVERIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAVE
YGLPVAVFSMEMPGTQLVMRMLGSIGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQLFIDETGGLNPMELRSRARRLAR
QCGKLGLIIVDYLQLMSGSSQGENRATEISEISRSLKSLAKELDVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDA
DVILFIYRDEVYNPDSPDKGTAEIIIGKQRNGPIGPVRLTFLGQYTKFDNFAGAQTFYGE

Sequences:

>Translated_460_residues
MNAPQDPQIESLKVPPHSVEAEQSVLGGLLLDNAAWDRIADFLSQGDFYRYDHRIIYEHIGRLIASTRPADVVTVYEALT
TSGKADDVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVLRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDG
ARGNQGFLEIGPLLTQVVERIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAVE
YGLPVAVFSMEMPGTQLVMRMLGSIGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQLFIDETGGLNPMELRSRARRLAR
QCGKLGLIIVDYLQLMSGSSQGENRATEISEISRSLKSLAKELDVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDA
DVILFIYRDEVYNPDSPDKGTAEIIIGKQRNGPIGPVRLTFLGQYTKFDNFAGAQTFYGE
>Mature_460_residues
MNAPQDPQIESLKVPPHSVEAEQSVLGGLLLDNAAWDRIADFLSQGDFYRYDHRIIYEHIGRLIASTRPADVVTVYEALT
TSGKADDVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVLRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDG
ARGNQGFLEIGPLLTQVVERIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAVE
YGLPVAVFSMEMPGTQLVMRMLGSIGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQLFIDETGGLNPMELRSRARRLAR
QCGKLGLIIVDYLQLMSGSSQGENRATEISEISRSLKSLAKELDVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDA
DVILFIYRDEVYNPDSPDKGTAEIIIGKQRNGPIGPVRLTFLGQYTKFDNFAGAQTFYGE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=453, Percent_Identity=56.5121412803532, Blast_Score=510, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50687; Mature: 50687

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPQDPQIESLKVPPHSVEAEQSVLGGLLLDNAAWDRIADFLSQGDFYRYDHRIIYEHI
CCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHH
GRLIASTRPADVVTVYEALTTSGKADDVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVL
HHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDGARGNQGFLEIGPLLTQVVER
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAVE
HHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCEEEEE
YGLPVAVFSMEMPGTQLVMRMLGSIGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQLFI
CCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEE
DETGGLNPMELRSRARRLARQCGKLGLIIVDYLQLMSGSSQGENRATEISEISRSLKSLA
ECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KELDVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDADVILFIYRDEVYNPDSPDKG
HHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCC
TAEIIIGKQRNGPIGPVRLTFLGQYTKFDNFAGAQTFYGE
CEEEEEECCCCCCCCCEEEEEEECHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNAPQDPQIESLKVPPHSVEAEQSVLGGLLLDNAAWDRIADFLSQGDFYRYDHRIIYEHI
CCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHH
GRLIASTRPADVVTVYEALTTSGKADDVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVL
HHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDGARGNQGFLEIGPLLTQVVER
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAVE
HHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCEEEEE
YGLPVAVFSMEMPGTQLVMRMLGSIGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQLFI
CCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEE
DETGGLNPMELRSRARRLARQCGKLGLIIVDYLQLMSGSSQGENRATEISEISRSLKSLA
ECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KELDVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDADVILFIYRDEVYNPDSPDKG
HHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCC
TAEIIIGKQRNGPIGPVRLTFLGQYTKFDNFAGAQTFYGE
CEEEEEECCCCCCCCCEEEEEEECHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]