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The map label for this gene is pit [H]

Identifier: 78066637

GI number: 78066637

Start: 2216000

End: 2217010

Strand: Reverse

Name: pit [H]

Synonym: Bcep18194_A5168

Alternate gene names: 78066637

Gene position: 2217010-2216000 (Counterclockwise)

Preceding gene: 78066638

Following gene: 78066625

Centisome position: 60.01

GC content: 65.18

Gene sequence:

>1011_bases
ATGCATTCGATACAACTCGCCCTATGGATGGTCGCGACGCTGGTGCTCGTCGCGCTCGTATTCGACTTCATGAACGGCTT
TCACGACGCGGCGAACTCGATCGCCACCGTCGTGTCCACCGGGGTGCTGAAACCCCAGCAGGCCGTCGTGTTCGCGGCGG
CGTTCAACGTCATCGCGTATTTCATCTTTCACCTGAAAGTCGCGCAGACCGTCGGTAAAGGCACGATCGATCCCGAGATC
GTCGACCACTACGTCGTGTTCGGTGCGCTCGTCGGTGCGATCGGCTGGAACGTGATCACGTGGTACTACGGGATCCCGTC
GAGCTCGTCGCATGCGCTGATCGGCGGCCTCGTCGGCGCGGCGCTCGCCAAGTCGGGCTGGAGCTCGCTGAACATCGACG
GGCTGCTGAAGACCATCGCGTTCATCTTCATCTCGCCGCTGCTCGGCTTCATCCTCGGCTCGCTGTTCATGCTCGGCGTG
TCGTGGCTGTATTTCCGTACCGCGCCCAGCAAGGTCGACCGGCGCTTCCGCCGGCTGCAGCTGCTGTCGGCCGGGCTGTA
CAGCCTCGGCCACGGCGGCAACGACGCGCAGAAGACGATCGGCATCATCTGGATGCTGCTGATCGCGTCGGGCTATGCGT
CGGCGACCGCCGATGCACCGCCGGCGTGGGTGATCGGCGCGTGCTACCTGTCGATGGGCCTCGGTACGCTGTTCGGCGGC
TGGCGCATCGTGCGCACGATGGGCCAGAAGATCACGAAGCTCAAGCCGGTCGGTGGCTTCTGTGCGGAAACCGGCGGTGC
GATGACGCTGTTCCTCGCGTCGTTCCTCGGCATTCCTGTGTCGACCACGCACACGATCACCGGCGCGATCGTCGGCGTCG
GCGCGACGCAGAAGCTGTCGGCCGTGCGCTGGGGTGTGGCCGGCAACATCGTGTGGGCGTGGGTGCTGACGCTGCCCGCG
GCTGCGTTGTTCGCGGCCGGTGGATGGTGGCTCGGTCACCAGATCTTCTGA

Upstream 100 bases:

>100_bases
AAGGCGATCTACGAGCTGCTCGAAGAGATCACCGACAAGTGCGAGGACGTCGCGAACATCATCGAAGGCATCGTGCTGGA
AAACGCCTGAGCGGACCACG

Downstream 100 bases:

>100_bases
TCGCCGATTCGATACTGTCGAACGGACATGAAATGCGCCGCAAGCCGAAAGGCTGCGGCGCATTTTCTTTTGGGGTGCGG
GAAGGTGCGGCGAAGAGCGG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MHSIQLALWMVATLVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFIFHLKVAQTVGKGTIDPEI
VDHYVVFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGAALAKSGWSSLNIDGLLKTIAFIFISPLLGFILGSLFMLGV
SWLYFRTAPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASATADAPPAWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAETGGAMTLFLASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTLPA
AALFAAGGWWLGHQIF

Sequences:

>Translated_336_residues
MHSIQLALWMVATLVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFIFHLKVAQTVGKGTIDPEI
VDHYVVFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGAALAKSGWSSLNIDGLLKTIAFIFISPLLGFILGSLFMLGV
SWLYFRTAPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASATADAPPAWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAETGGAMTLFLASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTLPA
AALFAAGGWWLGHQIF
>Mature_336_residues
MHSIQLALWMVATLVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFIFHLKVAQTVGKGTIDPEI
VDHYVVFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGAALAKSGWSSLNIDGLLKTIAFIFISPLLGFILGSLFMLGV
SWLYFRTAPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASATADAPPAWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAETGGAMTLFLASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTLPA
AALFAAGGWWLGHQIF

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=158, Percent_Identity=37.3417721518987, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI31543630, Length=168, Percent_Identity=34.5238095238095, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=31.7972350230415, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1789360, Length=255, Percent_Identity=31.7647058823529, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17539280, Length=176, Percent_Identity=34.0909090909091, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI25146401, Length=163, Percent_Identity=31.2883435582822, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71981576, Length=163, Percent_Identity=29.4478527607362, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI32566716, Length=169, Percent_Identity=32.5443786982249, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17557328, Length=153, Percent_Identity=33.9869281045752, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=169, Percent_Identity=28.4023668639053, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319773, Length=172, Percent_Identity=31.3953488372093, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356511, Length=184, Percent_Identity=33.1521739130435, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35608; Mature: 35608

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSIQLALWMVATLVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FIFHLKVAQTVGKGTIDPEIVDHYVVFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
ALAKSGWSSLNIDGLLKTIAFIFISPLLGFILGSLFMLGVSWLYFRTAPSKVDRRFRRLQ
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASATADAPPAWVIGACYLSMGLGTLFGG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
WRIVRTMGQKITKLKPVGGFCAETGGAMTLFLASFLGIPVSTTHTITGAIVGVGATQKLS
HHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHEECCCHHHHH
AVRWGVAGNIVWAWVLTLPAAALFAAGGWWLGHQIF
HHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
>Mature Secondary Structure
MHSIQLALWMVATLVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FIFHLKVAQTVGKGTIDPEIVDHYVVFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
ALAKSGWSSLNIDGLLKTIAFIFISPLLGFILGSLFMLGVSWLYFRTAPSKVDRRFRRLQ
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASATADAPPAWVIGACYLSMGLGTLFGG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
WRIVRTMGQKITKLKPVGGFCAETGGAMTLFLASFLGIPVSTTHTITGAIVGVGATQKLS
HHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHEECCCHHHHH
AVRWGVAGNIVWAWVLTLPAAALFAAGGWWLGHQIF
HHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]