Click here to switch to the map view.

The map label for this gene is thiC [H]

Identifier: 78065802

GI number: 78065802

Start: 1283098

End: 1285029

Strand: Reverse

Name: thiC [H]

Synonym: Bcep18194_A4331

Alternate gene names: 78065802

Gene position: 1285029-1283098 (Counterclockwise)

Preceding gene: 78065806

Following gene: 78065801

Centisome position: 34.79

GC content: 65.06

Gene sequence:

>1932_bases
ATGAACGCCAATCCGAAGTTTCTGTCCGCCGACGCTCATGTCGATGCCGCGGCCGTCGCCCCGCTGCCGAATTCCCGCAA
GGTTTATGTAACCGGCTCCCAGCCCGACATCCGCGTGCCGATGCGTGAAATCACGCAGGCCGACACGCCGACCGGCTTCG
GCGGCGAAAAGAATCCGCCGATCTACGTGTACGACACGTCGGGCCCCTACACCGATCCGGAAGCGAAGATCGACATCCGC
GCAGGCCTGGCCGCGCTGCGTCAGGGCTGGATCGACGCACGCGGCGACACCGAAGTGCTCGGCGGCCTGTCGAGCGAGTA
CGGCCTCGAGCGCGCGGCCGACCCGGCCACCGCCGACCTGCGGTTCCCGGGCCTGCACCGCAACCCGCGCCGCGCCCAGG
CCGGCAAGAACGTCTCGCAGATGCACTACGCGCGCCAGGGCATCATCACGCCGGAAATGGAATACATCGCGATCCGCGAG
AACCAGCGCCGCGCCGAGTACCTCGAGAGCCTGAAGGCCAGCGGCCCGAACGGCGCGAAGCTCGCCGCGATGATGGGCCG
CCAGCACCCGGGCCAGGCGTTCGGCGCAGCGGCTTTCGGCGCGAACGCGCCGGCCGAGATCACGCCGGAATTCGTGCGCT
CGGAAGTGGCGTGCGGCCGCGCGATCATCCCCGCGAACATCAACCACCCGGAATCCGAGCCGATGATCATCGGCCGCAAC
TTCCTCGTGAAGATCAACGCGAACATCGGCAACTCGGCCGTCACGTCGTCGATCGGCGAGGAAGTCGACAAGATGACGTG
GGCGATCCGCTGGGGCGGCGACACGGTGATGGACCTGTCGACCGGCAAGCACATCCATGAAACGCGCGAGTGGATCATCC
GCAACAGCCCGGTGCCGATCGGCACGGTGCCGATCTACCAGGCGCTGGAAAAGGTCAACGGCAAGGCCGAGGATCTCACC
TGGGAAATCTTCCGCGACACGCTGATCGAACAGGCCGAGCAAGGCGTCGACTATTTCACGATCCACGCGGGCGTGCGCCT
GCAGTACGTGCCGCTCACCGCGAACCGGATGACCGGCATCGTGTCGCGCGGCGGCTCGATCATGGCGAAGTGGTGCCTCG
CGCATCACAAGGAAAGCTTCCTGTACGAACACTTCGAAGAGATCTGCGAGATCATGAAGGCGTACGACGTGAGCTTCTCG
CTCGGCGACGGCCTGCGCCCCGGATCGATCTACGACGCGAACGACGAAGCGCAGCTCGGCGAGCTGAAGACGCTCGGCGA
ACTCACGCAGATCGCGTGGAAGCACGACGTGCAGGTGATGATCGAAGGCCCCGGCCACGTGCCGATGCAGCTGATCAAGG
AGAACATGGATCTCCAGCTCGACTGGTGCAAGGAAGCGCCGTTCTACACGCTCGGGCCGCTGACCACCGACATCGCACCG
GGCTACGACCACATCACGTCCGGCATCGGCGCCGCGATGATCGGCTGGTTCGGCACCGCGATGCTGTGCTACGTGACGCC
GAAGGAACACCTCGGCCTGCCAAACAAGGACGACGTGAAGGAAGGCATCATCACGTACAAGCTCGCCGCGCACGCCGCCG
ACCTCGCGAAGGGTCACCCGGGCGCGCAGGTGCGCGACAACGCGCTGTCGAAGGCGCGCTTCGAGTTCCGCTGGGAAGAC
CAGTTCAACATCGGTCTCGATCCGGACAAGGCGCGCGAATTCCACGACGAAACGCTGCCGAAGGATTCGGCGAAGGTCGC
GCACTTCTGCTCGATGTGCGGCCCGCACTTCTGCTCGATGAAGATCACGCAGGACGTGCGCGAGTTCGCGGCGCAGCAGG
GCGTGTCGGAAACCGAAGCGCTGAAGAAGGGGATGGAAGTGAAAGCGGTCGAGTTCGTCAAGACCGGCGCCGAAATCTAT
CACCGTCAATAA

Upstream 100 bases:

>100_bases
CCTCACCAAGCCTCGTCTCCTGCTTAGCTCGAGCCCCACATACGTCAAGGGCTCCGGGCAAGCGCCGAAGCGCCTGTCCG
GCGCTCACACAGGAGATTGC

Downstream 100 bases:

>100_bases
GCAAACGCGCGGCCCGGCGATCCCCGCCGGATCGCGTCGCGATGCACGAAGCCCGCTTTCGAGCGGGCTTTTTTACGCAT
GTCGCGTGCCTGCCGGTGCA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPPIYVYDTSGPYTDPEAKIDIR
AGLAALRQGWIDARGDTEVLGGLSSEYGLERAADPATADLRFPGLHRNPRRAQAGKNVSQMHYARQGIITPEMEYIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHPGQAFGAAAFGANAPAEITPEFVRSEVACGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNIGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVREFAAQQGVSETEALKKGMEVKAVEFVKTGAEIY
HRQ

Sequences:

>Translated_643_residues
MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPPIYVYDTSGPYTDPEAKIDIR
AGLAALRQGWIDARGDTEVLGGLSSEYGLERAADPATADLRFPGLHRNPRRAQAGKNVSQMHYARQGIITPEMEYIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHPGQAFGAAAFGANAPAEITPEFVRSEVACGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNIGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVREFAAQQGVSETEALKKGMEVKAVEFVKTGAEIY
HRQ
>Mature_643_residues
MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPPIYVYDTSGPYTDPEAKIDIR
AGLAALRQGWIDARGDTEVLGGLSSEYGLERAADPATADLRFPGLHRNPRRAQAGKNVSQMHYARQGIITPEMEYIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHPGQAFGAAAFGANAPAEITPEFVRSEVACGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNIGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVREFAAQQGVSETEALKKGMEVKAVEFVKTGAEIY
HRQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=641, Percent_Identity=68.9547581903276, Blast_Score=910, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 70785; Mature: 70785

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPP
CCCCCCEEECCCCCCCEEECCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCC
IYVYDTSGPYTDPEAKIDIRAGLAALRQGWIDARGDTEVLGGLSSEYGLERAADPATADL
EEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHCCCCHHHCCHHCCCCCCCCC
RFPGLHRNPRRAQAGKNVSQMHYARQGIITPEMEYIAIRENQRRAEYLESLKASGPNGAK
CCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHH
LAAMMGRQHPGQAFGAAAFGANAPAEITPEFVRSEVACGRAIIPANINHPESEPMIIGRN
HHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCE
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPI
EEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCC
GTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGI
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHHHHHHH
VSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFSLGDGLRPGSIYDANDEAQLG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCCHHH
ELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLTTDIAP
HHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCC
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHP
CHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHEEEHHHHHHHHHHCCCC
GAQVRDNALSKARFEFRWEDQFNIGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSM
CCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHEEE
KITQDVREFAAQQGVSETEALKKGMEVKAVEFVKTGAEIYHRQ
HHHHHHHHHHHHCCCCHHHHHHCCCCEEHHHHHHHCHHHHCCC
>Mature Secondary Structure
MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPP
CCCCCCEEECCCCCCCEEECCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCC
IYVYDTSGPYTDPEAKIDIRAGLAALRQGWIDARGDTEVLGGLSSEYGLERAADPATADL
EEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHCCCCHHHCCHHCCCCCCCCC
RFPGLHRNPRRAQAGKNVSQMHYARQGIITPEMEYIAIRENQRRAEYLESLKASGPNGAK
CCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHH
LAAMMGRQHPGQAFGAAAFGANAPAEITPEFVRSEVACGRAIIPANINHPESEPMIIGRN
HHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCE
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPI
EEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCC
GTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGI
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHHHHHHH
VSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFSLGDGLRPGSIYDANDEAQLG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCCHHH
ELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLTTDIAP
HHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCC
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHP
CHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHEEEHHHHHHHHHHCCCC
GAQVRDNALSKARFEFRWEDQFNIGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSM
CCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHEEE
KITQDVREFAAQQGVSETEALKKGMEVKAVEFVKTGAEIYHRQ
HHHHHHHHHHHHCCCCHHHHHHCCCCEEHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA