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The map label for this gene is ybfI [H]

Identifier: 78065682

GI number: 78065682

Start: 1144803

End: 1145702

Strand: Reverse

Name: ybfI [H]

Synonym: Bcep18194_A4210

Alternate gene names: 78065682

Gene position: 1145702-1144803 (Counterclockwise)

Preceding gene: 78065683

Following gene: 78065681

Centisome position: 31.01

GC content: 69.78

Gene sequence:

>900_bases
ATGTCCGCCACCCGCCTTCCAGACTCCGCACGCTACTGGCGCACGCCGCTGCTGCCGGATGCGGATCTCGTCACGGCCAC
CTATCGCGACCACACGTTCGCGCCCCACTGGCACGATGCGTACACGATCCCCGTGATTCTCGAAGGCGCGGAACGCTTCA
CCTATCGCGGCAGCGGCTATGTCGCCGAAACCGGCTCCGTGCCCGTCATCAATCCCGGTGAGGTGCATACGGGTTCGCGC
GCGGCCGACGAGGGCTGGTGCTATCGCGTCAGCTACATGCCGGTCGAATTCATCCGCGAACTCACCAGCGCGATCGCCGG
CCGCCCGCAGGACGCGCCGTGGTTTGCGCCCGACGTGATTCGCGATGCCGATCTCGCAGCCAGGCTCGCGCTGGCGCACC
GGATGATGGAGGCCGGCAGCGAGCACTCCCTGCCGTCGCAGCCGCACGGGCAGCCGGCTGACTATCCGATGACGGGCGTA
CCGCGCATCTACGATCCGCTCGCCGCCGAAACGGCGATGCTGGATGCGTTGTCGACGCTGATCGTGCGCCATGCCGACGC
ACTGCCGCGACCCGCGCCGCTCGCAGCGGACGAACCGCGCGTCGACGCGATGCGCGAGTGCCTCGCCGCCGATCTCATGT
GCACGGTCACGCTGGACGAAGTCGCACAGGCGGCCGGCCTGTCGCCGTTCCATGCAGCGCGCCTGTTCACCCGCACGACC
GGCATGCCGCCGCACGCGTGGCGCAACCAGTTGCGCTTGCAGCGTGCGCTGGCGCCGCTGCGCGCGGGCGTGCCCGTCGC
CGACGTCGCGGCGGCCAGCGGTTTCGTCGACCAAAGCCACTTCACCCGGCATTTCAAACGCATGTTCGGCGTGCCGCCCG
GGCGCTGGCAGGCGAGCTGA

Upstream 100 bases:

>100_bases
TACCGATGCCGGCTGGCACGACGATTCCGGTCAAGTAAAGCCTGCGCCTGCCGGGCGCGCGAGCGATACACTGTCGGTTC
GACCGCTTCCGCGCCCGATC

Downstream 100 bases:

>100_bases
CGCGCAGCGTCCGGCCCATCGCCGCGATACCGCCGGCACCGCTGCCTCCCGCAAGCGCAAGAACATACAAGCCGGCACAC
CCCCTGCCCGCTATCCTCCA

Product: AraC family transcriptional regulator

Products: DNA lacking O-methylguanine; protein S-methyl-L-cysteine [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSATRLPDSARYWRTPLLPDADLVTATYRDHTFAPHWHDAYTIPVILEGAERFTYRGSGYVAETGSVPVINPGEVHTGSR
AADEGWCYRVSYMPVEFIRELTSAIAGRPQDAPWFAPDVIRDADLAARLALAHRMMEAGSEHSLPSQPHGQPADYPMTGV
PRIYDPLAAETAMLDALSTLIVRHADALPRPAPLAADEPRVDAMRECLAADLMCTVTLDEVAQAAGLSPFHAARLFTRTT
GMPPHAWRNQLRLQRALAPLRAGVPVADVAAASGFVDQSHFTRHFKRMFGVPPGRWQAS

Sequences:

>Translated_299_residues
MSATRLPDSARYWRTPLLPDADLVTATYRDHTFAPHWHDAYTIPVILEGAERFTYRGSGYVAETGSVPVINPGEVHTGSR
AADEGWCYRVSYMPVEFIRELTSAIAGRPQDAPWFAPDVIRDADLAARLALAHRMMEAGSEHSLPSQPHGQPADYPMTGV
PRIYDPLAAETAMLDALSTLIVRHADALPRPAPLAADEPRVDAMRECLAADLMCTVTLDEVAQAAGLSPFHAARLFTRTT
GMPPHAWRNQLRLQRALAPLRAGVPVADVAAASGFVDQSHFTRHFKRMFGVPPGRWQAS
>Mature_298_residues
SATRLPDSARYWRTPLLPDADLVTATYRDHTFAPHWHDAYTIPVILEGAERFTYRGSGYVAETGSVPVINPGEVHTGSRA
ADEGWCYRVSYMPVEFIRELTSAIAGRPQDAPWFAPDVIRDADLAARLALAHRMMEAGSEHSLPSQPHGQPADYPMTGVP
RIYDPLAAETAMLDALSTLIVRHADALPRPAPLAADEPRVDAMRECLAADLMCTVTLDEVAQAAGLSPFHAARLFTRTTG
MPPHAWRNQLRLQRALAPLRAGVPVADVAAASGFVDQSHFTRHFKRMFGVPPGRWQAS

Specific function: Repair Of Alkylated Guanine In DNA By Stoichiometrically Transferring The Alkyl Group At The O-6 Position To A Cysteine Residue In The Enzyme. This Is A Suicide Reaction: The Enzyme Is Irreversibly Inactivated. Can Also Repair O-4-Methylthymine. The Meth

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018060 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63 [C]

Molecular weight: Translated: 32797; Mature: 32665

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATRLPDSARYWRTPLLPDADLVTATYRDHTFAPHWHDAYTIPVILEGAERFTYRGSGY
CCCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCHHEEECCCCE
VAETGSVPVINPGEVHTGSRAADEGWCYRVSYMPVEFIRELTSAIAGRPQDAPWFAPDVI
EECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHH
RDADLAARLALAHRMMEAGSEHSLPSQPHGQPADYPMTGVPRIYDPLAAETAMLDALSTL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IVRHADALPRPAPLAADEPRVDAMRECLAADLMCTVTLDEVAQAAGLSPFHAARLFTRTT
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEHHHHHHHHCCCHHHHHHHHHHCC
GMPPHAWRNQLRLQRALAPLRAGVPVADVAAASGFVDQSHFTRHFKRMFGVPPGRWQAS
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SATRLPDSARYWRTPLLPDADLVTATYRDHTFAPHWHDAYTIPVILEGAERFTYRGSGY
CCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCHHEEECCCCE
VAETGSVPVINPGEVHTGSRAADEGWCYRVSYMPVEFIRELTSAIAGRPQDAPWFAPDVI
EECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHH
RDADLAARLALAHRMMEAGSEHSLPSQPHGQPADYPMTGVPRIYDPLAAETAMLDALSTL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IVRHADALPRPAPLAADEPRVDAMRECLAADLMCTVTLDEVAQAAGLSPFHAARLFTRTT
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEHHHHHHHHCCCHHHHHHHHHHCC
GMPPHAWRNQLRLQRALAPLRAGVPVADVAAASGFVDQSHFTRHFKRMFGVPPGRWQAS
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing O-methylguanine; protein L-cysteine; DNA [C]

Specific reaction: DNA containing O-methylguanine + protein L-cysteine = DNA lacking O-methylguanine + protein S-methyl-L-cysteine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]