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The map label for this gene is thyA

Identifier: 78065671

GI number: 78065671

Start: 1133023

End: 1133994

Strand: Direct

Name: thyA

Synonym: Bcep18194_A4199

Alternate gene names: 78065671

Gene position: 1133023-1133994 (Clockwise)

Preceding gene: 78065670

Following gene: 78065674

Centisome position: 30.67

GC content: 65.12

Gene sequence:

>972_bases
ATGAAACAGTATCTCGACCTCGTTCGTACCATTCTCGATACCGGCACCTGGCAGGAGAACCGCACGGGAATCCGCACCAT
CAGCATGCCGGGCGCGATGCTGCGCTTCGACCTGCAGCAAGGCTTCCCGGCCGTGACGACCAAGAAGCTCGCGTTCAAGT
CCGCGATCGGTGAACTGGTCGGCTTCCTGCGCGCGTCGCGCAGCGCGGCCGATTTCCGCGCTCTCGGCTGCAAGGTGTGG
GACGCGAACGCGAACGAGAACGCGCAATGGCTCGCGAACCCGTATCGCCAGGGCGTCGACGATCTCGGTGACGTGTACGG
CGTGCAATGGCGCCAGTGGCCGGGCTACAAGGTGCTCGACGCGGGTGCCGATGCGCAGATCGACGATGCGACGCGCCGCG
GCTTCCGGATCGTCACGCGTTTCGACGAAGACGGCGCGCCGAAGGTGCTGCTTTACAAGGCGATCGACCAGTTGCGCCAG
TGCCTGGATACGATCATGGAGAACCCGGCCGACCGTCGCATCCTGTTTCACGCGTGGAATCCGGCCGTGCTCGACCAGAT
CGCGCTGCCCGCATGCCATCTGCTGTACCAGTTCCTGCCGAATGCGACGAAGCGCGAAATCTCGCTGTGCCTGTACATCC
GCAGCAACGACGTCGGCCTCGGCACGCCGTTCAACCTGACGGAAGGGGCCGCGCTGCTGGAACTCGTCGGCCGGCTGACG
GGCTATACGCCGCGCTGGTTCACGTACTTCATCGGCGACGCCCATATCTACGAGAACCAGCTCGACATGCTGCAGCGCCA
GCTCACGCGCGAGCCTTACGACAGCCCGCGGCTCGAGATTGCCGAGCGCGTGCCGGAATACGCGAAGACGGGCGTCTACG
CGCCCGAGTGGCTGGAGCAGATCGAGCCGTCCGATTTCTCGCTCGTCGGCTACCGCCACCATGAGCCGCTGACCGCGCCG
ATGGCGGTCTGA

Upstream 100 bases:

>100_bases
GCGACACGCTGGCAGGGTTTTCGGCGCCGTTACCGGAGCTTCCGCATCACGCTTGCTAAAATTGGCGGGTACGGCCGCCC
TCGCGGCCGAAGGAACCCGC

Downstream 100 bases:

>100_bases
TCGCGCGACTCGGGCCGCGCGGATGCATCGATCCGCGCCCCGCAGGAACAAGGCCCGACCGGCTGATACCGGTCGGGCCT
TTTTGTTTCACGTTTGCGGC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRASRSAADFRALGCKVW
DANANENAQWLANPYRQGVDDLGDVYGVQWRQWPGYKVLDAGADAQIDDATRRGFRIVTRFDEDGAPKVLLYKAIDQLRQ
CLDTIMENPADRRILFHAWNPAVLDQIALPACHLLYQFLPNATKREISLCLYIRSNDVGLGTPFNLTEGAALLELVGRLT
GYTPRWFTYFIGDAHIYENQLDMLQRQLTREPYDSPRLEIAERVPEYAKTGVYAPEWLEQIEPSDFSLVGYRHHEPLTAP
MAV

Sequences:

>Translated_323_residues
MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRASRSAADFRALGCKVW
DANANENAQWLANPYRQGVDDLGDVYGVQWRQWPGYKVLDAGADAQIDDATRRGFRIVTRFDEDGAPKVLLYKAIDQLRQ
CLDTIMENPADRRILFHAWNPAVLDQIALPACHLLYQFLPNATKREISLCLYIRSNDVGLGTPFNLTEGAALLELVGRLT
GYTPRWFTYFIGDAHIYENQLDMLQRQLTREPYDSPRLEIAERVPEYAKTGVYAPEWLEQIEPSDFSLVGYRHHEPLTAP
MAV
>Mature_323_residues
MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRASRSAADFRALGCKVW
DANANENAQWLANPYRQGVDDLGDVYGVQWRQWPGYKVLDAGADAQIDDATRRGFRIVTRFDEDGAPKVLLYKAIDQLRQ
CLDTIMENPADRRILFHAWNPAVLDQIALPACHLLYQFLPNATKREISLCLYIRSNDVGLGTPFNLTEGAALLELVGRLT
GYTPRWFTYFIGDAHIYENQLDMLQRQLTREPYDSPRLEIAERVPEYAKTGVYAPEWLEQIEPSDFSLVGYRHHEPLTAP
MAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=321, Percent_Identity=35.202492211838, Blast_Score=187, Evalue=8e-48,
Organism=Escherichia coli, GI1789191, Length=323, Percent_Identity=34.0557275541796, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71993377, Length=322, Percent_Identity=31.3664596273292, Blast_Score=157, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6324648, Length=274, Percent_Identity=32.4817518248175, Blast_Score=142, Evalue=7e-35,
Organism=Drosophila melanogaster, GI17137556, Length=321, Percent_Identity=35.202492211838, Blast_Score=194, Evalue=7e-50,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36718; Mature: 36718

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELV
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHEEEEHHCCCCCHHHHHHHHHHHHHHHH
GFLRASRSAADFRALGCKVWDANANENAQWLANPYRQGVDDLGDVYGVQWRQWPGYKVLD
HHHHHCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHCCCEECCCCCEEEE
AGADAQIDDATRRGFRIVTRFDEDGAPKVLLYKAIDQLRQCLDTIMENPADRRILFHAWN
CCCCCCCCHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
PAVLDQIALPACHLLYQFLPNATKREISLCLYIRSNDVGLGTPFNLTEGAALLELVGRLT
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
GYTPRWFTYFIGDAHIYENQLDMLQRQLTREPYDSPRLEIAERVPEYAKTGVYAPEWLEQ
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHCCCCCHHHHHH
IEPSDFSLVGYRHHEPLTAPMAV
CCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELV
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHEEEEHHCCCCCHHHHHHHHHHHHHHHH
GFLRASRSAADFRALGCKVWDANANENAQWLANPYRQGVDDLGDVYGVQWRQWPGYKVLD
HHHHHCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHCCCEECCCCCEEEE
AGADAQIDDATRRGFRIVTRFDEDGAPKVLLYKAIDQLRQCLDTIMENPADRRILFHAWN
CCCCCCCCHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
PAVLDQIALPACHLLYQFLPNATKREISLCLYIRSNDVGLGTPFNLTEGAALLELVGRLT
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
GYTPRWFTYFIGDAHIYENQLDMLQRQLTREPYDSPRLEIAERVPEYAKTGVYAPEWLEQ
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHCCCCCHHHHHH
IEPSDFSLVGYRHHEPLTAPMAV
CCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]