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The map label for this gene is yhjG [H]

Identifier: 78065614

GI number: 78065614

Start: 1074077

End: 1075717

Strand: Direct

Name: yhjG [H]

Synonym: Bcep18194_A4142

Alternate gene names: 78065614

Gene position: 1074077-1075717 (Clockwise)

Preceding gene: 78065612

Following gene: 78065619

Centisome position: 29.08

GC content: 70.63

Gene sequence:

>1641_bases
ATGGCTGACACACTGTCCGATATCCCGCCCGTGCTGATCGTCGGCGCGGGGCCGACCGGCCTTGCCGCGGCGATGAGCCT
CGCGCGGGCCCGCGTGCCGGTGCGCATCATCGATCGGCTCGCTACGCCTGCGCCGCATTCGCGCGCGATCGGCATCCAGG
CGCGCACGCTCGAACTGCTGGAGCAGCATCGCGCGGTCGAACCGTTTCTCGCGCTCGGTCATCGTGCGCATGCGGCCGCG
CTGCATGCCGACGGCCGCGTGATCGCGCGGCTCGATTTCGATCCGCTGCAAACGCGCTATCCGTATCTGCTGTTTCTCGA
CCAGACCGTCACCGAGCGCCTGCTTGCCGAGCACCTGGCCCGGCTCGGCGTGACGGTCGAGCGCGGCGCGACGCTGACCG
CGTGCGATGCGGGCGGCGCGTCGCTCGACGTGTCGGTCCGCCGCGCCGACGGCCGCGACGAATCGTTCGCGCCGTCGTAC
CTGATCGCTGCCGACGGCGCGCACAGCACGGTCAGGCATCTGCTCGGCCTGGGCTTCACCGGGCATGCGTTCGAACAGAC
CTTCCTGCTGGCCGATTTCGCGGCGATACCCGACTGGCCGGACGAAGAGATCCACCTGTTCACGACATCCGAAGGTATTG
CGGGCCTGTTTCCGATGGGCGGCGGCCGTTACCGGCTCGTGGCCGACCGGCCGCCCGGCAGCGATGCGTCGCCCGATGCG
CCAACCCCGTCGCTCGCGGAATGCGACGCGATCGTGCGTACCCGTGCCGGCGCGTCGATCTCGCCGAGCGATCTCGCGTG
GTCGTCCTATTTCCACCTGCACACCCGGATGATCGACCGGCTGCGTCACGGCCGCGTGTTCTTCGCCGGCGACGCCGCGC
ACGTCCACAGCCCGGCCGGCGCGCAGGGCATGAACACCGGCATCCAGGAGGCGTTCAATCTCGGCTGGAAGCTCGCGCGC
GTGCTTGGCGCGGGCACGCCCGAGCGGCTGCTCGACACGTATCACGCGGAGCGTCATCCGATCGAGCGCGACGGGTTGCG
GCAGACCGGTTTCGTCACGCAGATCGTCGAAGCCGAGCGTGGTGCGATGAAGCTGCTGCGCGATCACGTCGTGCCGGTGC
TGGCGTCGTTCGGGCCGATGCGGGACGCGGTGCGGCGCACGGTCAGCGAGCTCGGCGTGCAGTACCGGAAGAGTCCGCTC
ACGCTGGAGCGTGTGCTCGACGGCGGGCCGCGCGCAGGCGAGCGGGCGCCGGACGCGCTCGTGCGGGTGCTCGACGGGCC
GCTCGGTCAGGCGCCCGGAACGGCGCGGCTATACGACCTGCACGATCCGGCGAGTTTTACGTTGCTGCTGCTGGAGGAGC
CGGTGAATGCCGACGGCGCGGTGCCGCCGATGCCGGCCGATGCGCAGGCGCTCGTGCAGGGGCTCGAACGGATCATGCCG
GATGCGGTGCGCGCGTGGCGCGTCACCGATGCCGAAGGCGGCGATGGCGGGCTTGCGCAGGAATACGGCCGCTCGCGACC
GTCGTTTTACCTGCTACGGCCGGATGGTTACGTCGCCGCGCGCGGGCGCACGGCGACCGATGCGAACGCGCTGCTGCGCC
ACTGCGAAAGCTGGTTCGCGGGCATGTCGCAGTCAGCGTAG

Upstream 100 bases:

>100_bases
TCCCACACCGCGCGGCGGTGCCGCGGATCGGCCGATCGGCATATGGCCCCGTCACGCTGAGCGGCGTAGACTTGCAGGGT
CGCGTGTCATCGAGGTTCTC

Downstream 100 bases:

>100_bases
CGGTGCGGTCAGGCGCTCGCCGCGGCAGGGACGAGCGACGCGCGGTGCACGGCGATCGCGTCGCGCACGATCGGCGCCGC
GCGTTGCGCGGCTTCGCCCG

Product: monooxygenase, FAD-binding

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MADTLSDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLATPAPHSRAIGIQARTLELLEQHRAVEPFLALGHRAHAAA
LHADGRVIARLDFDPLQTRYPYLLFLDQTVTERLLAEHLARLGVTVERGATLTACDAGGASLDVSVRRADGRDESFAPSY
LIAADGAHSTVRHLLGLGFTGHAFEQTFLLADFAAIPDWPDEEIHLFTTSEGIAGLFPMGGGRYRLVADRPPGSDASPDA
PTPSLAECDAIVRTRAGASISPSDLAWSSYFHLHTRMIDRLRHGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLAR
VLGAGTPERLLDTYHAERHPIERDGLRQTGFVTQIVEAERGAMKLLRDHVVPVLASFGPMRDAVRRTVSELGVQYRKSPL
TLERVLDGGPRAGERAPDALVRVLDGPLGQAPGTARLYDLHDPASFTLLLLEEPVNADGAVPPMPADAQALVQGLERIMP
DAVRAWRVTDAEGGDGGLAQEYGRSRPSFYLLRPDGYVAARGRTATDANALLRHCESWFAGMSQSA

Sequences:

>Translated_546_residues
MADTLSDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLATPAPHSRAIGIQARTLELLEQHRAVEPFLALGHRAHAAA
LHADGRVIARLDFDPLQTRYPYLLFLDQTVTERLLAEHLARLGVTVERGATLTACDAGGASLDVSVRRADGRDESFAPSY
LIAADGAHSTVRHLLGLGFTGHAFEQTFLLADFAAIPDWPDEEIHLFTTSEGIAGLFPMGGGRYRLVADRPPGSDASPDA
PTPSLAECDAIVRTRAGASISPSDLAWSSYFHLHTRMIDRLRHGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLAR
VLGAGTPERLLDTYHAERHPIERDGLRQTGFVTQIVEAERGAMKLLRDHVVPVLASFGPMRDAVRRTVSELGVQYRKSPL
TLERVLDGGPRAGERAPDALVRVLDGPLGQAPGTARLYDLHDPASFTLLLLEEPVNADGAVPPMPADAQALVQGLERIMP
DAVRAWRVTDAEGGDGGLAQEYGRSRPSFYLLRPDGYVAARGRTATDANALLRHCESWFAGMSQSA
>Mature_545_residues
ADTLSDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLATPAPHSRAIGIQARTLELLEQHRAVEPFLALGHRAHAAAL
HADGRVIARLDFDPLQTRYPYLLFLDQTVTERLLAEHLARLGVTVERGATLTACDAGGASLDVSVRRADGRDESFAPSYL
IAADGAHSTVRHLLGLGFTGHAFEQTFLLADFAAIPDWPDEEIHLFTTSEGIAGLFPMGGGRYRLVADRPPGSDASPDAP
TPSLAECDAIVRTRAGASISPSDLAWSSYFHLHTRMIDRLRHGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLARV
LGAGTPERLLDTYHAERHPIERDGLRQTGFVTQIVEAERGAMKLLRDHVVPVLASFGPMRDAVRRTVSELGVQYRKSPLT
LERVLDGGPRAGERAPDALVRVLDGPLGQAPGTARLYDLHDPASFTLLLLEEPVNADGAVPPMPADAQALVQGLERIMPD
AVRAWRVTDAEGGDGGLAQEYGRSRPSFYLLRPDGYVAARGRTATDANALLRHCESWFAGMSQSA

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=338, Percent_Identity=26.6272189349112, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 58730; Mature: 58599

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTLSDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLATPAPHSRAIGIQARTLELL
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEECCHHHHHHHH
EQHRAVEPFLALGHRAHAAALHADGRVIARLDFDPLQTRYPYLLFLDQTVTERLLAEHLA
HHHCCHHHHHHHCCHHHHHEEECCCEEEEEECCCCHHCCCCEEEEECHHHHHHHHHHHHH
RLGVTVERGATLTACDAGGASLDVSVRRADGRDESFAPSYLIAADGAHSTVRHLLGLGFT
HHCCEEECCCEEEEECCCCCEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCCC
GHAFEQTFLLADFAAIPDWPDEEIHLFTTSEGIAGLFPMGGGRYRLVADRPPGSDASPDA
CHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCCCCEEEEEECCCCCCCCCCCC
PTPSLAECDAIVRTRAGASISPSDLAWSSYFHLHTRMIDRLRHGRVFFAGDAAHVHSPAG
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCC
AQGMNTGIQEAFNLGWKLARVLGAGTPERLLDTYHAERHPIERDGLRQTGFVTQIVEAER
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
GAMKLLRDHVVPVLASFGPMRDAVRRTVSELGVQYRKSPLTLERVLDGGPRAGERAPDAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHH
VRVLDGPLGQAPGTARLYDLHDPASFTLLLLEEPVNADGAVPPMPADAQALVQGLERIMP
HHHHCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
DAVRAWRVTDAEGGDGGLAQEYGRSRPSFYLLRPDGYVAARGRTATDANALLRHCESWFA
HHHHHEEEECCCCCCCCHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHH
GMSQSA
CCCCCC
>Mature Secondary Structure 
ADTLSDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLATPAPHSRAIGIQARTLELL
CCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEECCHHHHHHHH
EQHRAVEPFLALGHRAHAAALHADGRVIARLDFDPLQTRYPYLLFLDQTVTERLLAEHLA
HHHCCHHHHHHHCCHHHHHEEECCCEEEEEECCCCHHCCCCEEEEECHHHHHHHHHHHHH
RLGVTVERGATLTACDAGGASLDVSVRRADGRDESFAPSYLIAADGAHSTVRHLLGLGFT
HHCCEEECCCEEEEECCCCCEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCCC
GHAFEQTFLLADFAAIPDWPDEEIHLFTTSEGIAGLFPMGGGRYRLVADRPPGSDASPDA
CHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCCCCEEEEEECCCCCCCCCCCC
PTPSLAECDAIVRTRAGASISPSDLAWSSYFHLHTRMIDRLRHGRVFFAGDAAHVHSPAG
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCC
AQGMNTGIQEAFNLGWKLARVLGAGTPERLLDTYHAERHPIERDGLRQTGFVTQIVEAER
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
GAMKLLRDHVVPVLASFGPMRDAVRRTVSELGVQYRKSPLTLERVLDGGPRAGERAPDAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHH
VRVLDGPLGQAPGTARLYDLHDPASFTLLLLEEPVNADGAVPPMPADAQALVQGLERIMP
HHHHCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
DAVRAWRVTDAEGGDGGLAQEYGRSRPSFYLLRPDGYVAARGRTATDANALLRHCESWFA
HHHHHEEEECCCCCCCCHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHH
GMSQSA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]