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The map label for this gene is purH

Identifier: 78065253

GI number: 78065253

Start: 671739

End: 673304

Strand: Reverse

Name: purH

Synonym: Bcep18194_A3777

Alternate gene names: 78065253

Gene position: 673304-671739 (Counterclockwise)

Preceding gene: 78065254

Following gene: 78065252

Centisome position: 18.23

GC content: 65.77

Gene sequence:

>1566_bases
ATGATCAAGCAAGCGCTCATTTCCGTTTCCGACAAGACCGGCATCGTCGACTTCGCGAAGTCGCTGTCCGACCTCGGCGT
CAAGCTGCTGTCGACGGGCGGCACCGCGAAACTGCTCGCCGACGCGGGCCTGCCCGTGACCGAAGTGGCTGATTACACGG
GCTTCCCGGAAATGCTCGATGGGCGCGTGAAGACGCTCCACCCGAAGGTGCACGGCGGCATCCTGGCCCGCCGCGACCTG
CCCGAGCACATGCAGGCGCTGGAACAGCACGGTATCCCGACGATCGACCTGCTGGTCGTGAACCTGTACCCGTTCGTCGC
GACGATCGCGAAGGACGACTGCACGCTCGCCGACGCGATCGAGAACATCGACATCGGCGGCCCGACGATGCTGCGCTCGG
CTGCGAAGAACCACCGCGACGTGACGGTTGTTGTCGATCCGGCCGATTACGCGGTCGTGCTCGATGAAATGAAGGCGAAC
GGCAATGCGGTCGGCTACGCGACCAACTTCCGCCTCGCGACGAAGGTGTTCGCGCACACCGCGCAATACGACGGCGCGAT
CACGAACTACCTGACGAGCCTGACCGACGAGCTGAAGCACGCATCGCGCAGCGCGTACCCGGCGACGCTGAACCTGGCGT
TCGACAAGGTGCAGGACCTGCGCTACGGCGAGAACCCGCACCAGAGCGCCGCGTTCTACCGTGACCTCGCGACGCCGGCC
GGCGCGCTGGCGAACTACCGCCAGCTGCAGGGCAAGGAACTGTCGTACAACAACATCGCCGATTCGGACGCAGCGTGGGA
ATGCGTGAAGACGTTCGACGCACCGGCCTGCGTGATCATCAAGCATGCGAACCCGTGCGGCGTCGCGGTCGGCAACGATT
CGGCCGACGCGTACGCGAAGGCGTTCCAGACGGACCCGACGTCGGCATTCGGCGGCATCATCGCGTTCAACCGCGAAGTG
GACGAAGCGGCGGCCCAGGCCGTTGCGAAGCAGTTCGTCGAAGTGCTGATCGCACCGTCGTTCTCCGACGCCGCGAAGCA
GGTGTTCGCCGCGAAGCAGAACGTGCGCCTGCTCGAAATCGCGCTCGGCGACGGCCATAACGCGTTCGACCTGAAGCGCG
TGGGCGGCGGCCTGCTCGTGCAGTCGCTCGACTCGAAGAACGTGCAGCCGAGCGAACTGCGCGTCGTCACGAAGCGCCAG
CCGACCGCGAAGGAAATGGACGACCTGCTGTTCGCATGGCGTGTCGCGAAGTACGTGAAGTCGAACGCGATCGTGTTCTG
CGGCAACGGCATGACGCTCGGCGTCGGCGCAGGCCAGATGAGCCGTGTCGATTCCGCACGCATCGCGAGCATCAAGGCGC
AGAACGCAGGCCTGACGCTGGCCGGTTCGGCCGTGGCATCGGACGCGTTCTTCCCGTTCCGCGACGGCCTCGACGTCGTC
GTGGCGGCGGGCGCGACCTGCGTGATCCAGCCGGGCGGCTCGATGCGCGATGACGAAGTGATCGCTGCAGCCGACGAGCA
CAACATCGCGATGATCCTGACGGGCGTGCGCCACTTCCGTCACTGA

Upstream 100 bases:

>100_bases
TCGGCATCAATCGCAATACGCTGCGCAAGAAGCTGCAGCAGCACGGCCTGCTGTAGGCAGCCGTCCCGTCCCCGTTTCCC
TGGCTATTGGTGGTTCCATC

Downstream 100 bases:

>100_bases
TCGCACGCGGCCCGCGCGGCCGCTGCACGACAACCCGGCGGCATCGCACCCGCCGGGTTTTTTATTGCGCGGCGCCCTCG
TACGCGACCGTGCGGCCGGC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN
GNAVGYATNFRLATKVFAHTAQYDGAITNYLTSLTDELKHASRSAYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGDGHNAFDLKRVGGGLLVQSLDSKNVQPSELRVVTKRQ
PTAKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSMRDDEVIAAADEHNIAMILTGVRHFRH

Sequences:

>Translated_521_residues
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN
GNAVGYATNFRLATKVFAHTAQYDGAITNYLTSLTDELKHASRSAYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGDGHNAFDLKRVGGGLLVQSLDSKNVQPSELRVVTKRQ
PTAKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSMRDDEVIAAADEHNIAMILTGVRHFRH
>Mature_521_residues
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN
GNAVGYATNFRLATKVFAHTAQYDGAITNYLTSLTDELKHASRSAYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGDGHNAFDLKRVGGGLLVQSLDSKNVQPSELRVVTKRQ
PTAKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSMRDDEVIAAADEHNIAMILTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=478, Percent_Identity=37.4476987447699, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=62.2857142857143, Blast_Score=659, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=612, Percent_Identity=32.6797385620915, Blast_Score=284, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI71985574, Length=318, Percent_Identity=26.1006289308176, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=33.0769230769231, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323056, Length=488, Percent_Identity=37.2950819672131, Blast_Score=269, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6323768, Length=480, Percent_Identity=36.875, Blast_Score=268, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24649832, Length=485, Percent_Identity=38.1443298969072, Blast_Score=272, Evalue=4e-73,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55505; Mature: 55505

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLD
CCCHHHEECCCCCCHHHHHHHHHHCCEEEECCCCCEEEHCCCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKANGNAVGYATNFRLATKVFAHT
HCCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCCEEEEEECCHHHHHHHHHH
AQYDGAITNYLTSLTDELKHASRSAYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA
HHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCH
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCHHHHHH
AFQTDPTSAFGGIIAFNREVDEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEI
HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEE
ALGDGHNAFDLKRVGGGLLVQSLDSKNVQPSELRVVTKRQPTAKEMDDLLFAWRVAKYVK
EECCCCCCEEHHHCCCCCEEEECCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
CCEEEEECCCEEEEECCCCHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCEEE
VAAGATCVIQPGGSMRDDEVIAAADEHNIAMILTGVRHFRH
EECCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLD
CCCHHHEECCCCCCHHHHHHHHHHCCEEEECCCCCEEEHCCCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKANGNAVGYATNFRLATKVFAHT
HCCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCCEEEEEECCHHHHHHHHHH
AQYDGAITNYLTSLTDELKHASRSAYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA
HHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCH
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCHHHHHH
AFQTDPTSAFGGIIAFNREVDEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEI
HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEE
ALGDGHNAFDLKRVGGGLLVQSLDSKNVQPSELRVVTKRQPTAKEMDDLLFAWRVAKYVK
EECCCCCCEEHHHCCCCCEEEECCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
CCEEEEECCCEEEEECCCCHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCEEE
VAAGATCVIQPGGSMRDDEVIAAADEHNIAMILTGVRHFRH
EECCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA