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The map label for this gene is puuB [H]

Identifier: 78065227

GI number: 78065227

Start: 642338

End: 643759

Strand: Direct

Name: puuB [H]

Synonym: Bcep18194_A3751

Alternate gene names: 78065227

Gene position: 642338-643759 (Clockwise)

Preceding gene: 78065225

Following gene: 78065229

Centisome position: 17.39

GC content: 68.07

Gene sequence:

>1422_bases
ATGACTTTCAATAATCAACCCCTGATCCAGGGCGAATCGCGTTTCGAACAAACGCATCTGAACATCGAAAAAGACAACTG
GAACTGGTGCGACCCGGCCCGCTACGACGGCGAGCGCCCGGCCAACTGGTACGAGGCGTCGCTGTCGCGCCACGCGAACA
GTGCGCCGCTGGCACACGATGCCGTCTGCGACGTGCTCGTGATCGGCGCGGGCCTGCTCGGCGCGTCGGCCGCGCTGCAT
CTCGCGGAAGCGGGCGTCGATACCATCCTCGTCGACAAGCACCACGTCGGCTCGGCCGCGTCCGGCCGCAACGGCGGGCA
ACTGACGCCCGGCCTCGCGCGCTGGGAAGCCGCCGACATGATCGATCACCTGTCGCACGACGACGCGAAGCGGCTGTGGC
GCTTTGCGTCGTCCGAATCGATGGACCTGATCGATGCGATCGGCGCACGTTACGCGCTCGAGCTCGACCGCAAGCGCGGC
CACATTACGGCGGCGGTGCACCCGGGCCACATGAGCGCGCTGCTGGACGGCGCGGATGCGCGCCGCCATCTCGGCGACGC
GGGCGTGACGCTCGTCGGCCGCCATCAGCTGCATGACGAATACGTGCGCTCGGGGCTGTACCACGGCGCGGCGATCGATG
CGATCGGCGGGCAGATTCATCCGCTCGCGTTGGTGCGCGGCCTCGTGCACGGCTTCCGCCTGAACGGCGGCGCGCTGTTC
GAGGGCACCGAGGTGCTCGAGCTCGACGAGACGCCGGAGGGCGTCGTCGCAACGACGCCGGGCGGTACGATCACCGCGCG
CAAGGGCGTCGTGCTCGCGCTGCACAACACGACGTTCCGGCTGCTCGACGACGGTGCCGCAACGACCGTGCCGTTCTTTA
CCTACGTGAGCGTGACGGCGCCGCTCGACGTCGATGTCGCGACCCTGATGCCGGCCGGCATGCCCGTGTACGACACGCAG
TTCCAGATCGACTATTACCGGCCGGTGCGCGGCAACCGCCTACTGTTCGGCGGGCAGGGCACCGGCACGTGCTGGGCGCA
ACCGGACGTGAACGCGTACCTGCTGACGCGGCTGAACACGGTGTTTCCGCAGCACGACGGCCGGTTCGCGCTCGACTACT
GCTGGAGCGGCGTCAGCGACTTCACGCTGAACGGCGCGACCGACAGCCGCAAGACCGACGGCCGCGTGCCGATGTACATG
GTGCAGGGCTGGAGCGGCCACGGCGTCGCGCAGACGGTGCGGATCGGCAAGGCGATCTGCGACGATTTCGTCGGACGCAA
CGACGATTTCTCGATGCTGACCGGCATCGACCATCGCGCGATTCCGCTCGGCCGCCAGTTGTCGCCGATCGCGATTCCCG
CCGCGAAGGCCGCGATGAGCGTGATGAGCGCGCTCAACCCGGGGCGGATGATCTCGTTCTGA

Upstream 100 bases:

>100_bases
CGCGCCCCTTTTCACGTTGACACCCATGTGGAACCGGCTGCCCCACCCGCGAAGCCAGAAGGCTCTTCGACAGGCGGCCC
AAGACCATGCAAGTGCAATC

Downstream 100 bases:

>100_bases
CGCTGTTGCGGCAGGCAACAAAAAAGCCCGATGCGATTGCTCGCATCGGGCTTTTTTACGTGCTCGGACGGCGGCGCTTA
CGCGATCCGCTTCGCGAGTT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTFNNQPLIQGESRFEQTHLNIEKDNWNWCDPARYDGERPANWYEASLSRHANSAPLAHDAVCDVLVIGAGLLGASAALH
LAEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADMIDHLSHDDAKRLWRFASSESMDLIDAIGARYALELDRKRG
HITAAVHPGHMSALLDGADARRHLGDAGVTLVGRHQLHDEYVRSGLYHGAAIDAIGGQIHPLALVRGLVHGFRLNGGALF
EGTEVLELDETPEGVVATTPGGTITARKGVVLALHNTTFRLLDDGAATTVPFFTYVSVTAPLDVDVATLMPAGMPVYDTQ
FQIDYYRPVRGNRLLFGGQGTGTCWAQPDVNAYLLTRLNTVFPQHDGRFALDYCWSGVSDFTLNGATDSRKTDGRVPMYM
VQGWSGHGVAQTVRIGKAICDDFVGRNDDFSMLTGIDHRAIPLGRQLSPIAIPAAKAAMSVMSALNPGRMISF

Sequences:

>Translated_473_residues
MTFNNQPLIQGESRFEQTHLNIEKDNWNWCDPARYDGERPANWYEASLSRHANSAPLAHDAVCDVLVIGAGLLGASAALH
LAEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADMIDHLSHDDAKRLWRFASSESMDLIDAIGARYALELDRKRG
HITAAVHPGHMSALLDGADARRHLGDAGVTLVGRHQLHDEYVRSGLYHGAAIDAIGGQIHPLALVRGLVHGFRLNGGALF
EGTEVLELDETPEGVVATTPGGTITARKGVVLALHNTTFRLLDDGAATTVPFFTYVSVTAPLDVDVATLMPAGMPVYDTQ
FQIDYYRPVRGNRLLFGGQGTGTCWAQPDVNAYLLTRLNTVFPQHDGRFALDYCWSGVSDFTLNGATDSRKTDGRVPMYM
VQGWSGHGVAQTVRIGKAICDDFVGRNDDFSMLTGIDHRAIPLGRQLSPIAIPAAKAAMSVMSALNPGRMISF
>Mature_472_residues
TFNNQPLIQGESRFEQTHLNIEKDNWNWCDPARYDGERPANWYEASLSRHANSAPLAHDAVCDVLVIGAGLLGASAALHL
AEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADMIDHLSHDDAKRLWRFASSESMDLIDAIGARYALELDRKRGH
ITAAVHPGHMSALLDGADARRHLGDAGVTLVGRHQLHDEYVRSGLYHGAAIDAIGGQIHPLALVRGLVHGFRLNGGALFE
GTEVLELDETPEGVVATTPGGTITARKGVVLALHNTTFRLLDDGAATTVPFFTYVSVTAPLDVDVATLMPAGMPVYDTQF
QIDYYRPVRGNRLLFGGQGTGTCWAQPDVNAYLLTRLNTVFPQHDGRFALDYCWSGVSDFTLNGATDSRKTDGRVPMYMV
QGWSGHGVAQTVRIGKAICDDFVGRNDDFSMLTGIDHRAIPLGRQLSPIAIPAAKAAMSVMSALNPGRMISF

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=411, Percent_Identity=32.1167883211679, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51062; Mature: 50931

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNNQPLIQGESRFEQTHLNIEKDNWNWCDPARYDGERPANWYEASLSRHANSAPLAHD
CCCCCCCCCCCCHHHHHHCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHH
AVCDVLVIGAGLLGASAALHLAEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADM
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
IDHLSHDDAKRLWRFASSESMDLIDAIGARYALELDRKRGHITAAVHPGHMSALLDGADA
HHHHCCHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCCEEEEECCCHHHHHHCCCHH
RRHLGDAGVTLVGRHQLHDEYVRSGLYHGAAIDAIGGQIHPLALVRGLVHGFRLNGGALF
HHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHEEECCCEEE
EGTEVLELDETPEGVVATTPGGTITARKGVVLALHNTTFRLLDDGAATTVPFFTYVSVTA
CCCEEEEECCCCCCEEEECCCCEEEECCCEEEEEECCEEEEEECCCCCEEEEEEEEEEEC
PLDVDVATLMPAGMPVYDTQFQIDYYRPVRGNRLLFGGQGTGTCWAQPDVNAYLLTRLNT
CCCCCHHHHCCCCCCEECCEEEEEEECCCCCCEEEECCCCCCEEEECCCCCEEHEEHHHH
VFPQHDGRFALDYCWSGVSDFTLNGATDSRKTDGRVPMYMVQGWSGHGVAQTVRIGKAIC
HCCCCCCCEEEEHHHCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
DDFVGRNDDFSMLTGIDHRAIPLGRQLSPIAIPAAKAAMSVMSALNPGRMISF
HHHCCCCCCCHHEECCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TFNNQPLIQGESRFEQTHLNIEKDNWNWCDPARYDGERPANWYEASLSRHANSAPLAHD
CCCCCCCCCCCHHHHHHCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHH
AVCDVLVIGAGLLGASAALHLAEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADM
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
IDHLSHDDAKRLWRFASSESMDLIDAIGARYALELDRKRGHITAAVHPGHMSALLDGADA
HHHHCCHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCCEEEEECCCHHHHHHCCCHH
RRHLGDAGVTLVGRHQLHDEYVRSGLYHGAAIDAIGGQIHPLALVRGLVHGFRLNGGALF
HHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHEEECCCEEE
EGTEVLELDETPEGVVATTPGGTITARKGVVLALHNTTFRLLDDGAATTVPFFTYVSVTA
CCCEEEEECCCCCCEEEECCCCEEEECCCEEEEEECCEEEEEECCCCCEEEEEEEEEEEC
PLDVDVATLMPAGMPVYDTQFQIDYYRPVRGNRLLFGGQGTGTCWAQPDVNAYLLTRLNT
CCCCCHHHHCCCCCCEECCEEEEEEECCCCCCEEEECCCCCCEEEECCCCCEEHEEHHHH
VFPQHDGRFALDYCWSGVSDFTLNGATDSRKTDGRVPMYMVQGWSGHGVAQTVRIGKAIC
HCCCCCCCEEEEHHHCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
DDFVGRNDDFSMLTGIDHRAIPLGRQLSPIAIPAAKAAMSVMSALNPGRMISF
HHHCCCCCCCHHEECCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]