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The map label for this gene is truB [H]

Identifier: 78048391

GI number: 78048391

Start: 3224909

End: 3225835

Strand: Reverse

Name: truB [H]

Synonym: XCV2835

Alternate gene names: 78048391

Gene position: 3225835-3224909 (Counterclockwise)

Preceding gene: 78048392

Following gene: 78048390

Centisome position: 62.29

GC content: 66.02

Gene sequence:

>927_bases
TTGAAACCCCGCATCGTCTATCGCCCGTTGCACGGCATCCTGCTGCTGGACAAGCCGGCCGGGCTGAGCTCCAACAATGC
ACTGCAGGCCGCACGCCGGTTATTGCGTGCAGAGAAGGGCGGCCATACCGGCAGCCTGGATCCGCTGGCTACCGGCCTGT
TGCCGCTCTGCTTTGGCGAAGCCACCAAGATCGCCGGACTGCTGCTTGGTTCAGCCAAGGCATACGACGCCGAGATCGTG
TTGGGCGTCACCACCGACACCGACGATGCCGACGGCCAGCCGCTGCGCGAGCGTGCGGTGCCGGATCTGAGCGAGGCCGA
TCTACAGGCGGCGCTGGCGCCTTTCATCGGCCGTATCCAGCAACAAGCGCCCATTTATTCCGCGCTCAAGCAGGGCGGCG
AGCCGCTGTACGCCAAGGCGCGACGCGGCGAACGTATCGAAGCGCCGGTGCGCGAGGTGGATGTGCAGGCGATCGATGTG
CTGGGCTACAGCGCCCCAGGTCTGCGGCTGCGCGTGACCTGCGGCTCGGGCACGTATATCCGCAGCCTGGCACGTGACCT
GGGCGAGGTGCTGGGATGTGGAGCGCACATCGCCTCGTTGCGTCGGCTGTGGGTGGAGCCGTTTCGCGCTCCACAGATGA
TCACGCTCGAGGCATTGTCGGCGGCGCTGGAGGCGGGCACCGAAGCACGGACGCTATTGCTGCCGATCGAGGCCGGCCTG
GCCGGTTTTGCGCGAATCGTGCTCGATCAGACCCATGCGGCGCGTTTTCGCATGGGCCAGCGCCTGCGTGATGCCGCATT
TCCGCCGGGGCAAGTCGCTGTATTCGGACCTGACGGAACCCCCTCGGGGCTAGGCCTGGTCGACGCCGACGGGCGCTTGT
CGCCGCAGCGATTGTTCAATGGACTGAACGAAATTCCGGCCTGTTAA

Upstream 100 bases:

>100_bases
GAAGATGCCGAACAGCGCTGAGCGCTGTCACGGCCTGCCGCATCTGCCGCGGCCCTGATTCCCGCAGGCGCGCACTGCGC
CTGATTCGAGCACCGATCTT

Downstream 100 bases:

>100_bases
GATTTTGTCAACTTGTTCCCTGGGCCGGGCGACGTTACAATTTCGCGGCCCCGATCGGGGCACCTCAGCGCCAAGCGCGC
TCATCCTAAGCAAACGGCGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKPRIVYRPLHGILLLDKPAGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGEATKIAGLLLGSAKAYDAEIV
LGVTTDTDDADGQPLRERAVPDLSEADLQAALAPFIGRIQQQAPIYSALKQGGEPLYAKARRGERIEAPVREVDVQAIDV
LGYSAPGLRLRVTCGSGTYIRSLARDLGEVLGCGAHIASLRRLWVEPFRAPQMITLEALSAALEAGTEARTLLLPIEAGL
AGFARIVLDQTHAARFRMGQRLRDAAFPPGQVAVFGPDGTPSGLGLVDADGRLSPQRLFNGLNEIPAC

Sequences:

>Translated_308_residues
MKPRIVYRPLHGILLLDKPAGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGEATKIAGLLLGSAKAYDAEIV
LGVTTDTDDADGQPLRERAVPDLSEADLQAALAPFIGRIQQQAPIYSALKQGGEPLYAKARRGERIEAPVREVDVQAIDV
LGYSAPGLRLRVTCGSGTYIRSLARDLGEVLGCGAHIASLRRLWVEPFRAPQMITLEALSAALEAGTEARTLLLPIEAGL
AGFARIVLDQTHAARFRMGQRLRDAAFPPGQVAVFGPDGTPSGLGLVDADGRLSPQRLFNGLNEIPAC
>Mature_308_residues
MKPRIVYRPLHGILLLDKPAGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGEATKIAGLLLGSAKAYDAEIV
LGVTTDTDDADGQPLRERAVPDLSEADLQAALAPFIGRIQQQAPIYSALKQGGEPLYAKARRGERIEAPVREVDVQAIDV
LGYSAPGLRLRVTCGSGTYIRSLARDLGEVLGCGAHIASLRRLWVEPFRAPQMITLEALSAALEAGTEARTLLLPIEAGL
AGFARIVLDQTHAARFRMGQRLRDAAFPPGQVAVFGPDGTPSGLGLVDADGRLSPQRLFNGLNEIPAC

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=216, Percent_Identity=35.6481481481481, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI2367200, Length=307, Percent_Identity=42.0195439739414, Blast_Score=218, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6324037, Length=173, Percent_Identity=32.3699421965318, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=258, Percent_Identity=29.0697674418605, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI281364189, Length=259, Percent_Identity=25.4826254826255, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364187, Length=259, Percent_Identity=25.4826254826255, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364185, Length=259, Percent_Identity=25.4826254826255, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364183, Length=259, Percent_Identity=25.4826254826255, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI62471759, Length=259, Percent_Identity=25.4826254826255, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17975520, Length=259, Percent_Identity=25.4826254826255, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32676; Mature: 32676

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRIVYRPLHGILLLDKPAGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGE
CCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
ATKIAGLLLGSAKAYDAEIVLGVTTDTDDADGQPLRERAVPDLSEADLQAALAPFIGRIQ
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHH
QQAPIYSALKQGGEPLYAKARRGERIEAPVREVDVQAIDVLGYSAPGLRLRVTCGSGTYI
HCCHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHHHHHEEECCCCCCCEEEEEECCCHHH
RSLARDLGEVLGCGAHIASLRRLWVEPFRAPQMITLEALSAALEAGTEARTLLLPIEAGL
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCEEEEEEHHHHH
AGFARIVLDQTHAARFRMGQRLRDAAFPPGQVAVFGPDGTPSGLGLVDADGRLSPQRLFN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCCCHHHHHC
GLNEIPAC
CHHHCCCC
>Mature Secondary Structure
MKPRIVYRPLHGILLLDKPAGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGE
CCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
ATKIAGLLLGSAKAYDAEIVLGVTTDTDDADGQPLRERAVPDLSEADLQAALAPFIGRIQ
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHH
QQAPIYSALKQGGEPLYAKARRGERIEAPVREVDVQAIDVLGYSAPGLRLRVTCGSGTYI
HCCHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHHHHHEEECCCCCCCEEEEEECCCHHH
RSLARDLGEVLGCGAHIASLRRLWVEPFRAPQMITLEALSAALEAGTEARTLLLPIEAGL
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCEEEEEEHHHHH
AGFARIVLDQTHAARFRMGQRLRDAAFPPGQVAVFGPDGTPSGLGLVDADGRLSPQRLFN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCCCHHHHHC
GLNEIPAC
CHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]