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The map label for this gene is acsE [H]

Identifier: 78044202

GI number: 78044202

Start: 1093760

End: 1094551

Strand: Direct

Name: acsE [H]

Synonym: CHY_1227

Alternate gene names: 78044202

Gene position: 1093760-1094551 (Clockwise)

Preceding gene: 78042962

Following gene: 78042839

Centisome position: 45.54

GC content: 44.32

Gene sequence:

>792_bases
ATGTTTATAATGATCGGTGAGCGTATTAACGGAATGTTTAAGGACATTCGGGAGGCTATTTTAAATAAGGACCCACGTCC
GATTCAAGAATGGGCGAGAAGGCAGGCTGAGAAAGGTGCCCATTACTTGGATGTTAATACCGGTCCAACTGCCGATGATC
CGGTGCGCGTAATGGAATGGCTGGTAAAAACAATTCAAGAAGTTGTTGATTTACCTTGTTGCCTTGATTCTACTAACCCT
GATGCCATCGAAGCCGGTTTAAAGGTTCACCGGGGTCATGCTATGATAAACTCTACTTCGGCAGATCAGTGGAAAATGGA
TATATTCTTCCCCATGGCCAAAAAATACGAAGCAGCGATTATCGGTCTTACCATGAATGAAAAGGGCGTGCCCAAAGATG
CCAATGACCGTTCCCAATTGGCAATGGAACTTGTGGCAAATGCTGATGCTCATGGTATTCCTATGACTGAGCTTTATATT
GACCCTCTTATTTTGCCGGTAAACGTTGCCCAGGAACATGCGGTTGAGGTATTGGAAACTATCCGCCAAATTAAACTAAT
GGCTAATCCGGCTCCAAGAACAGTTCTGGGCTTAAGCAACGTTTCCCAAAAATGCCCGGATCGGCCGCTTATCAATAGAA
CTTATCTGGTTATGGCTATGACTGCCGGCTTAGATGCGGCAATAATGGATGTTGATGATGATGCCCTGGTAGATGCGGCG
GCCACTGCTCACATCTTGCTTAATAAAGAAATTTACTGCGATTCTTATCTTAAAACCTTTAGACAGAAGTAA

Upstream 100 bases:

>100_bases
GGGAGGGCACCTCTTTGTTATGCGCCATCCCAAGTCTTTGGCTGAAGTTAAAGAACATCTAAAAAGAATTTTAAAATAAT
TTACAGGAGGGAAATTTAAG

Downstream 100 bases:

>100_bases
GAGGACGGGGCTCTGCCCCGTCTTAATACCATTTATTTAATTCAATTTAAGGCGAGGGATTGTAATGGCCAATAACACCT
TAACTATGGAAATTGCAGAA

Product: methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MFIMIGERINGMFKDIREAILNKDPRPIQEWARRQAEKGAHYLDVNTGPTADDPVRVMEWLVKTIQEVVDLPCCLDSTNP
DAIEAGLKVHRGHAMINSTSADQWKMDIFFPMAKKYEAAIIGLTMNEKGVPKDANDRSQLAMELVANADAHGIPMTELYI
DPLILPVNVAQEHAVEVLETIRQIKLMANPAPRTVLGLSNVSQKCPDRPLINRTYLVMAMTAGLDAAIMDVDDDALVDAA
ATAHILLNKEIYCDSYLKTFRQK

Sequences:

>Translated_263_residues
MFIMIGERINGMFKDIREAILNKDPRPIQEWARRQAEKGAHYLDVNTGPTADDPVRVMEWLVKTIQEVVDLPCCLDSTNP
DAIEAGLKVHRGHAMINSTSADQWKMDIFFPMAKKYEAAIIGLTMNEKGVPKDANDRSQLAMELVANADAHGIPMTELYI
DPLILPVNVAQEHAVEVLETIRQIKLMANPAPRTVLGLSNVSQKCPDRPLINRTYLVMAMTAGLDAAIMDVDDDALVDAA
ATAHILLNKEIYCDSYLKTFRQK
>Mature_263_residues
MFIMIGERINGMFKDIREAILNKDPRPIQEWARRQAEKGAHYLDVNTGPTADDPVRVMEWLVKTIQEVVDLPCCLDSTNP
DAIEAGLKVHRGHAMINSTSADQWKMDIFFPMAKKYEAAIIGLTMNEKGVPKDANDRSQLAMELVANADAHGIPMTELYI
DPLILPVNVAQEHAVEVLETIRQIKLMANPAPRTVLGLSNVSQKCPDRPLINRTYLVMAMTAGLDAAIMDVDDDALVDAA
ATAHILLNKEIYCDSYLKTFRQK

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=254, Percent_Identity=27.1653543307087, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1790450, Length=258, Percent_Identity=27.1317829457364, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 29326; Mature: 29326

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIMIGERINGMFKDIREAILNKDPRPIQEWARRQAEKGAHYLDVNTGPTADDPVRVMEW
CEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
LVKTIQEVVDLPCCLDSTNPDAIEAGLKVHRGHAMINSTSADQWKMDIFFPMAKKYEAAI
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCHHCCCEEE
IGLTMNEKGVPKDANDRSQLAMELVANADAHGIPMTELYIDPLILPVNVAQEHAVEVLET
EEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEECCHHHHHHHHHHHH
IRQIKLMANPAPRTVLGLSNVSQKCPDRPLINRTYLVMAMTAGLDAAIMDVDDDALVDAA
HHHHHHHCCCCCCEEEEHHHHHHHCCCCCCCCHHEEEEEECCCCCEEEEECCCHHHHHHH
ATAHILLNKEIYCDSYLKTFRQK
HHHHHEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFIMIGERINGMFKDIREAILNKDPRPIQEWARRQAEKGAHYLDVNTGPTADDPVRVMEW
CEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
LVKTIQEVVDLPCCLDSTNPDAIEAGLKVHRGHAMINSTSADQWKMDIFFPMAKKYEAAI
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCHHCCCEEE
IGLTMNEKGVPKDANDRSQLAMELVANADAHGIPMTELYIDPLILPVNVAQEHAVEVLET
EEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEECCHHHHHHHHHHHH
IRQIKLMANPAPRTVLGLSNVSQKCPDRPLINRTYLVMAMTAGLDAAIMDVDDDALVDAA
HHHHHHHCCCCCCEEEEHHHHHHHCCCCCCCCHHEEEEEECCCCCEEEEECCCHHHHHHH
ATAHILLNKEIYCDSYLKTFRQK
HHHHHEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]