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The map label for this gene is hisZ

Identifier: 78044079

GI number: 78044079

Start: 959807

End: 960781

Strand: Direct

Name: hisZ

Synonym: CHY_1083

Alternate gene names: 78044079

Gene position: 959807-960781 (Clockwise)

Preceding gene: 78043299

Following gene: 78044817

Centisome position: 39.97

GC content: 41.13

Gene sequence:

>975_bases
ATGAGGGAAAACTTTAAGGAACTACCAATGGGAGTAAGAGATTATCTACCCGGGGAAGCAGCGTTACGGAGATACCTGGA
AAACTTGTTTGTAGAGCTGGTGGCCAAGGAAGGCTTTCAAGAGGTTATAACTCCTTCCTTTGAGTATTATGAAACTTTGC
GGGAATCATCGGAGACCGATTTAATTAAATTTGTGGACCGTACGGGAGAGCTTTTGGCCCTCAGGGGAGATATGACCACC
CCCATTGCCCGGGTGGTTTCTACCAAAATGAAGCATCGGGAGTTACCCCTTAAAATTTTTTATGCGGCAACGGTTTTTTC
CGGGAATTTTTCCGGAAGAAACAAACTTAGAGAGTTTAAACAACTGGGGATTGAAGTGTTTGGGGATATTACTTCAAAAT
TAGAATCAGACTTAATAAAACTAATGGCTGTGTATTTGCGTAAAGCCAAGGTGAAAAACCTGGAAATTTCTCTGGGACAT
GTGGATATTTTAACGGGGCTTATTGAAGAATACGAACTTTTTAACCATTTTTCGGAAATAAAAAATGCTTTAAATCAAAA
AGATTACGTAGCTTTGGAGGGAATTTTTGAGAGGGCGGGAAAAAGTCAAAGGGTCCGGGAAAGTCTGGTGGATTTTTTAC
AGCAGCGGGGAGGTAAAGCAATCCTAAGAAAGGCAAAGAAAATGTTAAGCCATCCGAAAGCCAAAAAAGCCTTAGAAGAA
CTGGAGGAGGTTACCGAATACCTAATTTCTGAGGGAGTAAGGGAATTAAAGCTGGATTTTTCTTTGGTCAGGGATTTAAA
CTATTACACCGGTTTGGTGTTTGAAGCTTACACCCCTTATTTAGGCTATCCTTTGGGGGGTGGAGGGCGTTACGATACCC
TTTTAAAAAAATTTGGCTGGGATACACCGGCTTTTGGTTTTGCCCTGGGGTTGGAAAGAATTGTTGAAAGTATTGCCAAT
AGTATTGACAAATAA

Upstream 100 bases:

>100_bases
GAGCAAGTACCGCTACCATTAGCCGGGTTAAAAGATATTTAAATTACGGCGCTTCCGGTTACCGGCTGATGCTTAAACGC
TTAAAGGAAGAAGGGAAATT

Downstream 100 bases:

>100_bases
TTTTTTTAACCTTATGATTTTAGTATATTAATAATTTAACACTTTAACAAAATAAAGTGAGGCTATAAAAATGAAAAATG
GTTTAACTTTTGCCCTGCCT

Product: putative ATP phosphoribosyltransferase, regulatory subunit

Products: AMP; diphosphate; L-histidyl-tRNA(His)

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MRENFKELPMGVRDYLPGEAALRRYLENLFVELVAKEGFQEVITPSFEYYETLRESSETDLIKFVDRTGELLALRGDMTT
PIARVVSTKMKHRELPLKIFYAATVFSGNFSGRNKLREFKQLGIEVFGDITSKLESDLIKLMAVYLRKAKVKNLEISLGH
VDILTGLIEEYELFNHFSEIKNALNQKDYVALEGIFERAGKSQRVRESLVDFLQQRGGKAILRKAKKMLSHPKAKKALEE
LEEVTEYLISEGVRELKLDFSLVRDLNYYTGLVFEAYTPYLGYPLGGGGRYDTLLKKFGWDTPAFGFALGLERIVESIAN
SIDK

Sequences:

>Translated_324_residues
MRENFKELPMGVRDYLPGEAALRRYLENLFVELVAKEGFQEVITPSFEYYETLRESSETDLIKFVDRTGELLALRGDMTT
PIARVVSTKMKHRELPLKIFYAATVFSGNFSGRNKLREFKQLGIEVFGDITSKLESDLIKLMAVYLRKAKVKNLEISLGH
VDILTGLIEEYELFNHFSEIKNALNQKDYVALEGIFERAGKSQRVRESLVDFLQQRGGKAILRKAKKMLSHPKAKKALEE
LEEVTEYLISEGVRELKLDFSLVRDLNYYTGLVFEAYTPYLGYPLGGGGRYDTLLKKFGWDTPAFGFALGLERIVESIAN
SIDK
>Mature_324_residues
MRENFKELPMGVRDYLPGEAALRRYLENLFVELVAKEGFQEVITPSFEYYETLRESSETDLIKFVDRTGELLALRGDMTT
PIARVVSTKMKHRELPLKIFYAATVFSGNFSGRNKLREFKQLGIEVFGDITSKLESDLIKLMAVYLRKAKVKNLEISLGH
VDILTGLIEEYELFNHFSEIKNALNQKDYVALEGIFERAGKSQRVRESLVDFLQQRGGKAILRKAKKMLSHPKAKKALEE
LEEVTEYLISEGVRELKLDFSLVRDLNYYTGLVFEAYTPYLGYPLGGGGRYDTLLKKFGWDTPAFGFALGLERIVESIAN
SIDK

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI6996014, Length=337, Percent_Identity=29.3768545994065, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI15029520, Length=341, Percent_Identity=28.1524926686217, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71993686, Length=346, Percent_Identity=28.3236994219653, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71993693, Length=346, Percent_Identity=28.3236994219653, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325290, Length=362, Percent_Identity=25.1381215469613, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24643061, Length=347, Percent_Identity=24.7838616714697, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24643059, Length=366, Percent_Identity=23.224043715847, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_CARHZ (Q3AD55)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359929.1
- ProteinModelPortal:   Q3AD55
- STRING:   Q3AD55
- GeneID:   3727195
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1083
- NMPDR:   fig|246194.3.peg.1130
- TIGR:   CHY_1083
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   YREHVKS
- BioCyc:   CHYD246194:CHY_1083-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21

Molecular weight: Translated: 37040; Mature: 37040

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRENFKELPMGVRDYLPGEAALRRYLENLFVELVAKEGFQEVITPSFEYYETLRESSETD
CCCCHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHH
LIKFVDRTGELLALRGDMTTPIARVVSTKMKHRELPLKIFYAATVFSGNFSGRNKLREFK
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHH
QLGIEVFGDITSKLESDLIKLMAVYLRKAKVKNLEISLGHVDILTGLIEEYELFNHFSEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHH
KNALNQKDYVALEGIFERAGKSQRVRESLVDFLQQRGGKAILRKAKKMLSHPKAKKALEE
HHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHH
LEEVTEYLISEGVRELKLDFSLVRDLNYYTGLVFEAYTPYLGYPLGGGGRYDTLLKKFGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHCC
DTPAFGFALGLERIVESIANSIDK
CCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRENFKELPMGVRDYLPGEAALRRYLENLFVELVAKEGFQEVITPSFEYYETLRESSETD
CCCCHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHH
LIKFVDRTGELLALRGDMTTPIARVVSTKMKHRELPLKIFYAATVFSGNFSGRNKLREFK
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHH
QLGIEVFGDITSKLESDLIKLMAVYLRKAKVKNLEISLGHVDILTGLIEEYELFNHFSEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHH
KNALNQKDYVALEGIFERAGKSQRVRESLVDFLQQRGGKAILRKAKKMLSHPKAKKALEE
HHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHH
LEEVTEYLISEGVRELKLDFSLVRDLNYYTGLVFEAYTPYLGYPLGGGGRYDTLLKKFGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHCC
DTPAFGFALGLERIVESIANSIDK
CCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; L-histidine; tRNA(His)

Specific reaction: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA