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The map label for this gene is cheB2

Identifier: 78043599

GI number: 78043599

Start: 911897

End: 912961

Strand: Direct

Name: cheB2

Synonym: CHY_1031

Alternate gene names: 78043599

Gene position: 911897-912961 (Clockwise)

Preceding gene: 78044189

Following gene: 78043687

Centisome position: 37.97

GC content: 44.41

Gene sequence:

>1065_bases
TTGTCCAAAATTAGAGTTTTGATAGTCGACGATTCAGCGTTAATGCGCAAATATTTGCGGGAAATCCTGGAACAGGACCC
GGAAATAGAGGTAATAGCTGTTGCACGGAACGGACGGGAAGCAGTGGAGAAAGCTTTAGAATTGTCTCCGGATGTCATTA
CTATGGATATCAACATGCCGGAGATGGATGGTTTAACGGCTATCCAATATATTATGCTTCACCGTCCTACTCCGATTATT
GTTATTTCCTCATTAACGCAAAAGGGAGCATTAACAACCTTTGAAGCTTTGGAACTGGGTGCCGTCGATTACGTGGCCAA
GCCCGACGGCACCGTTTCCTTAAAAATTAAGGAAGTTGCCGAAGAAATATTACAAAAAGTAAAGGCAGTAGCAAAATCTC
GCGGCGTTGTTCGCTTAAAAGCGCGCAAAGAGCGGCCTTTTGTTCAAGAGGTTCCAAAAACTAGGCCGGTTAGCCCCGAT
ACTAGTTTTAAAAAATTAATCTTGATTGGGGTATCTACCGGTGGGCCTAAAGCTTTAATGGAAATAATTCCAAGACTTCC
GGGGAATTTAGATGCGTCTGTAGTTATTATTCAGCACATGCCGGAGAAATTTACCGCGTCCTTTGCTGCACGCTTAAATA
ATTATTCGGAACTTTACGTGAAAGAAGCAGAAGACGGAGAAAGCCTAGAGCGGGGTAAGGTTTTGGTAGCTCGCGGAGGA
ATTAATCTCAAGTTAGAACGTAAACTTGGCATCAATGTTGTACGGGTGCGCTATACCCCTCTTCCCCGGGAAACAATTTA
CTGGCCGTCGGTGGATGTTGCCTTTCGTTCTGCTTTAACCGTAATTGAACCGCACAGAATAATTGCAGTTTTACTTACCG
GTATGGGGGATGATGGAGCTCAAGCTATGGTAGAAATTAAACAAAAAGGCGGTTATACCATAGCTGAAAGTGAAGAGACA
GCAGTAGTTTGGGGAATGCCCCGGGAAGCTATTGAACGGGGTGGAGCTTCAGAAATCTTGCCGGTGTATCAAATTGCTGA
CCGTATAGTAGAGTTGGTTCGGTAA

Upstream 100 bases:

>100_bases
ATTGCTAAGATAAACGGTGGAGAACGTTTAATTCTGCTGCTGGATCTCAAGCATGTAATTAGCCGGGAAGAAAAAGAACA
AATTGAGGAGGCATTAGATA

Downstream 100 bases:

>100_bases
AAGAGGTGGCTGCATGAAAGTTTTAATCGTTGATGATTCCTTTACTGTTCGACAGTATTTTAAGGAAATTTTTCAAGGAT
TTAGTTGGGAAGTAGAAGAA

Product: protein-glutamate methylesterase CheB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSKIRVLIVDDSALMRKYLREILEQDPEIEVIAVARNGREAVEKALELSPDVITMDINMPEMDGLTAIQYIMLHRPTPII
VISSLTQKGALTTFEALELGAVDYVAKPDGTVSLKIKEVAEEILQKVKAVAKSRGVVRLKARKERPFVQEVPKTRPVSPD
TSFKKLILIGVSTGGPKALMEIIPRLPGNLDASVVIIQHMPEKFTASFAARLNNYSELYVKEAEDGESLERGKVLVARGG
INLKLERKLGINVVRVRYTPLPRETIYWPSVDVAFRSALTVIEPHRIIAVLLTGMGDDGAQAMVEIKQKGGYTIAESEET
AVVWGMPREAIERGGASEILPVYQIADRIVELVR

Sequences:

>Translated_354_residues
MSKIRVLIVDDSALMRKYLREILEQDPEIEVIAVARNGREAVEKALELSPDVITMDINMPEMDGLTAIQYIMLHRPTPII
VISSLTQKGALTTFEALELGAVDYVAKPDGTVSLKIKEVAEEILQKVKAVAKSRGVVRLKARKERPFVQEVPKTRPVSPD
TSFKKLILIGVSTGGPKALMEIIPRLPGNLDASVVIIQHMPEKFTASFAARLNNYSELYVKEAEDGESLERGKVLVARGG
INLKLERKLGINVVRVRYTPLPRETIYWPSVDVAFRSALTVIEPHRIIAVLLTGMGDDGAQAMVEIKQKGGYTIAESEET
AVVWGMPREAIERGGASEILPVYQIADRIVELVR
>Mature_353_residues
SKIRVLIVDDSALMRKYLREILEQDPEIEVIAVARNGREAVEKALELSPDVITMDINMPEMDGLTAIQYIMLHRPTPIIV
ISSLTQKGALTTFEALELGAVDYVAKPDGTVSLKIKEVAEEILQKVKAVAKSRGVVRLKARKERPFVQEVPKTRPVSPDT
SFKKLILIGVSTGGPKALMEIIPRLPGNLDASVVIIQHMPEKFTASFAARLNNYSELYVKEAEDGESLERGKVLVARGGI
NLKLERKLGINVVRVRYTPLPRETIYWPSVDVAFRSALTVIEPHRIIAVLLTGMGDDGAQAMVEIKQKGGYTIAESEETA
VVWGMPREAIERGGASEILPVYQIADRIVELVR

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=350, Percent_Identity=41.1428571428571, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1788550, Length=106, Percent_Identity=40.5660377358491, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788521, Length=104, Percent_Identity=36.5384615384615, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082052, Length=108, Percent_Identity=37.962962962963, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB2_CARHZ (Q3ADA6)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359878.1
- ProteinModelPortal:   Q3ADA6
- SMR:   Q3ADA6
- STRING:   Q3ADA6
- GeneID:   3728752
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1031
- NMPDR:   fig|246194.3.peg.785
- TIGR:   CHY_1031
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   PADFPWP
- ProtClustDB:   CLSK569149
- BioCyc:   CHYD246194:CHY_1031-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 39156; Mature: 39025

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY; PS00120 LIPASE_SER

Important sites: ACT_SITE 172-172 ACT_SITE 199-199 ACT_SITE 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIRVLIVDDSALMRKYLREILEQDPEIEVIAVARNGREAVEKALELSPDVITMDINMP
CCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCEEEEECCCC
EMDGLTAIQYIMLHRPTPIIVISSLTQKGALTTFEALELGAVDYVAKPDGTVSLKIKEVA
CCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCEEEEECCCCEEEEEHHHHH
EEILQKVKAVAKSRGVVRLKARKERPFVQEVPKTRPVSPDTSFKKLILIGVSTGGPKALM
HHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCEEEEEEEECCCCHHHHH
EIIPRLPGNLDASVVIIQHMPEKFTASFAARLNNYSELYVKEAEDGESLERGKVLVARGG
HHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCEEEEECC
INLKLERKLGINVVRVRYTPLPRETIYWPSVDVAFRSALTVIEPHRIIAVLLTGMGDDGA
CCEEEEECCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHCCCHHEEEEEEECCCCCHH
QAMVEIKQKGGYTIAESEETAVVWGMPREAIERGGASEILPVYQIADRIVELVR
HHHHHHHHCCCEEEECCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SKIRVLIVDDSALMRKYLREILEQDPEIEVIAVARNGREAVEKALELSPDVITMDINMP
CCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCEEEEECCCC
EMDGLTAIQYIMLHRPTPIIVISSLTQKGALTTFEALELGAVDYVAKPDGTVSLKIKEVA
CCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCEEEEECCCCEEEEEHHHHH
EEILQKVKAVAKSRGVVRLKARKERPFVQEVPKTRPVSPDTSFKKLILIGVSTGGPKALM
HHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCEEEEEEEECCCCHHHHH
EIIPRLPGNLDASVVIIQHMPEKFTASFAARLNNYSELYVKEAEDGESLERGKVLVARGG
HHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCEEEEECC
INLKLERKLGINVVRVRYTPLPRETIYWPSVDVAFRSALTVIEPHRIIAVLLTGMGDDGA
CCEEEEECCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHCCCHHEEEEEEECCCCCHH
QAMVEIKQKGGYTIAESEETAVVWGMPREAIERGGASEILPVYQIADRIVELVR
HHHHHHHHCCCEEEECCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA