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The map label for this gene is rimO

Identifier: 78043523

GI number: 78043523

Start: 1037859

End: 1039175

Strand: Direct

Name: rimO

Synonym: CHY_1164

Alternate gene names: 78043523

Gene position: 1037859-1039175 (Clockwise)

Preceding gene: 78044924

Following gene: 78044108

Centisome position: 43.22

GC content: 35.99

Gene sequence:

>1317_bases
TTGAAATATTTTATCCTTTCCTTGGGCTGTACCAAAAATCAAGCGGATAGTGAAGTAATAATGGGAATCCTGGAAAGTAA
AGGGTATGTGCGCTCTTTAAATCCGGAAGAAAGCGATTTATTAATTGTAAATACCTGTGGTTTTATTGCTGCGGCCATTG
AAGAAAGTATTGAGGAAATTTTAAATCTTGTTCACCTGAAAAAACCCGGACAAAAAATCCTGGTTGCCGGTTGTTTGGTC
CAAAGGGAGGGTAAAGAGTTAGCAAAACATTTACCGGAGGTTGACCTATTTTTTACTCCCCGGGAAATAAATAACCTTGA
TAAACTTTTAGCTGATTTAGGAGAAAATAATAAGTTAGTTTTAAGCGAGCCGGGATTTTTAAACTTGGAAAAAAAACCGC
GAGCTAAAAGCAATGAGGTCTATCGTTATATCAAGATTGCCGATGGCTGTGATAACCGTTGTACCTATTGTACTATACCG
GCAATTCGGGGTAAATACACCAGCAGGCCGCTTGATGATATTTTAGAAGAAATTAAAGATACCCTAAAACAGGGAATTAA
AGAAATCATTTTGGTAGCTCAGGATACCACCGCGTACGGTATTGATCTTTATGGGGAGTTTAAATTGGTTGAACTTTTAA
GAAAAATTGGCAGCATTAAAGGAAATTTTTGGGTAAGGTTGATGTATCTTTATCCCGATAAAATTACTCCCGAATTAATT
AACGAGATTAAAGAAAATCCCAAGGTAATTAAGTACGTCGATGTACCGTTACAGCATATTCATCCCGAAATTTTAAAGAA
AATGGGAAGGAAGGGAAGTAGTGAAGAAATTATATCAACCTTAGAAAGGCTCCGAAAAGAAATACCGGATATAACTATAA
GGACTACCTTTATAGTTGGATTTCCGGGAGAGACGGAAGAACAGTTTAACTATCTTTTAGATTTTGTTAAAAAGTTTAAA
TTTAATCGCCTCGGGGCTTTTCCCTATTATCGCGAAAAAGGTACTCCCGCAGCAAAAATGAAGGGCCAAATACCGAAAAA
AGTTAAGGAGCAACGTTATGAGAAATTAATGGAGGTGCAGCAAGAGATTTCCCTTAACTTAAACAAAGCTTTGGTAGGCA
AAAAGATTCCCGTCATTGTTGAAAAAAAGATAAGAGGAGAAAACCTTTACCTTGGGCGAACCTATATGGATGCCCCCGAA
ATTGACGGAATAATTGAAATTAAAGCGGAAAAAAGATTGAAAAAGGGTCAAATTATTAATGTTTTAATAACCGATTATGA
TATATACGATTTAAAAGGAGAGTTTATAAATGATTGA

Upstream 100 bases:

>100_bases
GGAGCGGTAGTGGTAACTGTAAATGGAGTGACCTACGGAACCCCAGGAAAGATGGGCCAGGTGGTTAAAATAACATATTA
AGGGTAAGAGGTGAAAGTAG

Downstream 100 bases:

>100_bases
TAATACTTTATTTTCTTTGGCCCAAGAAGTGCAGCGGTTGTTTATTGCTAAGAATTTAACTTTGGCAACGGCTGAATCGT
GCACCGGCGGACTTTTAGGA

Product: putative tRNA modifying protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MKYFILSLGCTKNQADSEVIMGILESKGYVRSLNPEESDLLIVNTCGFIAAAIEESIEEILNLVHLKKPGQKILVAGCLV
QREGKELAKHLPEVDLFFTPREINNLDKLLADLGENNKLVLSEPGFLNLEKKPRAKSNEVYRYIKIADGCDNRCTYCTIP
AIRGKYTSRPLDDILEEIKDTLKQGIKEIILVAQDTTAYGIDLYGEFKLVELLRKIGSIKGNFWVRLMYLYPDKITPELI
NEIKENPKVIKYVDVPLQHIHPEILKKMGRKGSSEEIISTLERLRKEIPDITIRTTFIVGFPGETEEQFNYLLDFVKKFK
FNRLGAFPYYREKGTPAAKMKGQIPKKVKEQRYEKLMEVQQEISLNLNKALVGKKIPVIVEKKIRGENLYLGRTYMDAPE
IDGIIEIKAEKRLKKGQIINVLITDYDIYDLKGEFIND

Sequences:

>Translated_438_residues
MKYFILSLGCTKNQADSEVIMGILESKGYVRSLNPEESDLLIVNTCGFIAAAIEESIEEILNLVHLKKPGQKILVAGCLV
QREGKELAKHLPEVDLFFTPREINNLDKLLADLGENNKLVLSEPGFLNLEKKPRAKSNEVYRYIKIADGCDNRCTYCTIP
AIRGKYTSRPLDDILEEIKDTLKQGIKEIILVAQDTTAYGIDLYGEFKLVELLRKIGSIKGNFWVRLMYLYPDKITPELI
NEIKENPKVIKYVDVPLQHIHPEILKKMGRKGSSEEIISTLERLRKEIPDITIRTTFIVGFPGETEEQFNYLLDFVKKFK
FNRLGAFPYYREKGTPAAKMKGQIPKKVKEQRYEKLMEVQQEISLNLNKALVGKKIPVIVEKKIRGENLYLGRTYMDAPE
IDGIIEIKAEKRLKKGQIINVLITDYDIYDLKGEFIND
>Mature_438_residues
MKYFILSLGCTKNQADSEVIMGILESKGYVRSLNPEESDLLIVNTCGFIAAAIEESIEEILNLVHLKKPGQKILVAGCLV
QREGKELAKHLPEVDLFFTPREINNLDKLLADLGENNKLVLSEPGFLNLEKKPRAKSNEVYRYIKIADGCDNRCTYCTIP
AIRGKYTSRPLDDILEEIKDTLKQGIKEIILVAQDTTAYGIDLYGEFKLVELLRKIGSIKGNFWVRLMYLYPDKITPELI
NEIKENPKVIKYVDVPLQHIHPEILKKMGRKGSSEEIISTLERLRKEIPDITIRTTFIVGFPGETEEQFNYLLDFVKKFK
FNRLGAFPYYREKGTPAAKMKGQIPKKVKEQRYEKLMEVQQEISLNLNKALVGKKIPVIVEKKIRGENLYLGRTYMDAPE
IDGIIEIKAEKRLKKGQIINVLITDYDIYDLKGEFIND

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=419, Percent_Identity=27.2076372315036, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=26.3485477178423, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI93277076, Length=408, Percent_Identity=28.6764705882353, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1787057, Length=449, Percent_Identity=38.0846325167038, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=29.438202247191, Blast_Score=204, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=27.2340425531915, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI71996771, Length=213, Percent_Identity=33.8028169014084, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI21356207, Length=421, Percent_Identity=28.0285035629454, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI19922432, Length=449, Percent_Identity=27.1714922048998, Blast_Score=148, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_CARHZ (Q3ACX5)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_360009.1
- ProteinModelPortal:   Q3ACX5
- SMR:   Q3ACX5
- STRING:   Q3ACX5
- GeneID:   3726572
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1164
- NMPDR:   fig|246194.3.peg.1215
- TIGR:   CHY_1164
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- BioCyc:   CHYD246194:CHY_1164-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50192; Mature: 50192

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYFILSLGCTKNQADSEVIMGILESKGYVRSLNPEESDLLIVNTCGFIAAAIEESIEEI
CEEEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCCCCCEEEEECHHHHHHHHHHHHHHH
LNLVHLKKPGQKILVAGCLVQREGKELAKHLPEVDLFFTPREINNLDKLLADLGENNKLV
HHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEE
LSEPGFLNLEKKPRAKSNEVYRYIKIADGCDNRCTYCTIPAIRGKYTSRPLDDILEEIKD
EECCCEECCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
TLKQGIKEIILVAQDTTAYGIDLYGEFKLVELLRKIGSIKGNFWVRLMYLYPDKITPELI
HHHHHHHHHEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHH
NEIKENPKVIKYVDVPLQHIHPEILKKMGRKGSSEEIISTLERLRKEIPDITIRTTFIVG
HHHHCCCCEEEEECCCHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEE
FPGETEEQFNYLLDFVKKFKFNRLGAFPYYREKGTPAAKMKGQIPKKVKEQRYEKLMEVQ
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHH
QEISLNLNKALVGKKIPVIVEKKIRGENLYLGRTYMDAPEIDGIIEIKAEKRLKKGQIIN
HHHCCCCCHHHHCCCCCEEEEECCCCCEEEEECEECCCCCCCCEEEEEHHHHCCCCCEEE
VLITDYDIYDLKGEFIND
EEEECCEEEECCCCCCCC
>Mature Secondary Structure
MKYFILSLGCTKNQADSEVIMGILESKGYVRSLNPEESDLLIVNTCGFIAAAIEESIEEI
CEEEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCCCCCEEEEECHHHHHHHHHHHHHHH
LNLVHLKKPGQKILVAGCLVQREGKELAKHLPEVDLFFTPREINNLDKLLADLGENNKLV
HHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEE
LSEPGFLNLEKKPRAKSNEVYRYIKIADGCDNRCTYCTIPAIRGKYTSRPLDDILEEIKD
EECCCEECCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
TLKQGIKEIILVAQDTTAYGIDLYGEFKLVELLRKIGSIKGNFWVRLMYLYPDKITPELI
HHHHHHHHHEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHH
NEIKENPKVIKYVDVPLQHIHPEILKKMGRKGSSEEIISTLERLRKEIPDITIRTTFIVG
HHHHCCCCEEEEECCCHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEE
FPGETEEQFNYLLDFVKKFKFNRLGAFPYYREKGTPAAKMKGQIPKKVKEQRYEKLMEVQ
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHH
QEISLNLNKALVGKKIPVIVEKKIRGENLYLGRTYMDAPEIDGIIEIKAEKRLKKGQIIN
HHHCCCCCHHHHCCCCCEEEEECCCCCEEEEECEECCCCCCCCEEEEEHHHHCCCCCEEE
VLITDYDIYDLKGEFIND
EEEECCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA