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The map label for this gene is yagI [H]

Identifier: 78043361

GI number: 78043361

Start: 1133418

End: 1134203

Strand: Direct

Name: yagI [H]

Synonym: CHY_1273

Alternate gene names: 78043361

Gene position: 1133418-1134203 (Clockwise)

Preceding gene: 78042657

Following gene: 78043263

Centisome position: 47.2

GC content: 38.8

Gene sequence:

>786_bases
ATGAAAAAAAACAATAACGATACTCTTATTCAATCGGTTGATCGGGCTTTAAGAATATTAGATACTTTTAGCCTTAAAGA
AAAGGAATTAGGTGTTACGGAAATTGCCAATCGTTTAGGCTTGCATAAAAGTACTGTCTTTGGGTTACTCAGGACCCTTG
AACATTGGGGATATGTTGAACAAAACCAAGTTACAGGAAAATACCGTCTAGGTCTTAAGTTATTAGAATTGGGTAATCGA
GTAAAAGAAGGCTTAGATTTACGGGCGGTAGCTTTGCCATTTTTACAGGATCTTGTTGAAAGATACGGTGAAACCGTTCA
TTTGGCTGTTCACGACCGGGGAGAAATTGTCTATATTGAAAAAGTGGAAGGACCAAATGCTATTCGAATGTACTCGCAAA
TTGGGCGACGGGCTCCAATGCATTGTACAGGTGTAGGTAAAGCTATCCTTGCTTTTAGACCCGAGAAGGAAATAGAGGAA
ATAATTCGCACCAAGGGGCTTAAATATTTTACTCCTAACACGATTACAGATCCCAAGAAACTACATGAAGAACTAAGTTT
GATTAGGGAAAATGGTTATAGTCTTGACAGAGAGGAAATTGAGATAGGGTTAAGATGCGTTGCAGCTCCTATCAGGGATA
GTCAAAATACAGTTGTTGCAGCTATTAGTGTTGCTGGTCCTTCAATACGCATGACCGAGGAGAAAATACAGGAATTAATT
GTTAGTGTTAAAGAAGCTGCCTTAGAAATATCAAAGCGTTTGGGCTATCAAACATCAAGTAGGTAG

Upstream 100 bases:

>100_bases
AAATTTTGAAAGGAAATTTAAGATTTATGTTGAAAATAATAACTTAAGTAAGGGAGGATACAAATTGATTAGGAGTTTTT
ATTGATGGAAGGTTGAAATA

Downstream 100 bases:

>100_bases
TTATATTTCGTAACAAATTAGTTTTATTGTGCCAATAAATCAAAACACAGTTTGATAATACAAAACAAAGCAAAGAAATG
TAAAGGAAGGGGGAAAGGCA

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKKNNNDTLIQSVDRALRILDTFSLKEKELGVTEIANRLGLHKSTVFGLLRTLEHWGYVEQNQVTGKYRLGLKLLELGNR
VKEGLDLRAVALPFLQDLVERYGETVHLAVHDRGEIVYIEKVEGPNAIRMYSQIGRRAPMHCTGVGKAILAFRPEKEIEE
IIRTKGLKYFTPNTITDPKKLHEELSLIRENGYSLDREEIEIGLRCVAAPIRDSQNTVVAAISVAGPSIRMTEEKIQELI
VSVKEAALEISKRLGYQTSSR

Sequences:

>Translated_261_residues
MKKNNNDTLIQSVDRALRILDTFSLKEKELGVTEIANRLGLHKSTVFGLLRTLEHWGYVEQNQVTGKYRLGLKLLELGNR
VKEGLDLRAVALPFLQDLVERYGETVHLAVHDRGEIVYIEKVEGPNAIRMYSQIGRRAPMHCTGVGKAILAFRPEKEIEE
IIRTKGLKYFTPNTITDPKKLHEELSLIRENGYSLDREEIEIGLRCVAAPIRDSQNTVVAAISVAGPSIRMTEEKIQELI
VSVKEAALEISKRLGYQTSSR
>Mature_261_residues
MKKNNNDTLIQSVDRALRILDTFSLKEKELGVTEIANRLGLHKSTVFGLLRTLEHWGYVEQNQVTGKYRLGLKLLELGNR
VKEGLDLRAVALPFLQDLVERYGETVHLAVHDRGEIVYIEKVEGPNAIRMYSQIGRRAPMHCTGVGKAILAFRPEKEIEE
IIRTKGLKYFTPNTITDPKKLHEELSLIRENGYSLDREEIEIGLRCVAAPIRDSQNTVVAAISVAGPSIRMTEEKIQELI
VSVKEAALEISKRLGYQTSSR

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=42.3387096774194, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1788131, Length=248, Percent_Identity=39.5161290322581, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI87082359, Length=248, Percent_Identity=35.8870967741936, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1786715, Length=248, Percent_Identity=36.2903225806452, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI1788581, Length=249, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789999, Length=249, Percent_Identity=30.1204819277108, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790752, Length=226, Percent_Identity=30.5309734513274, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI145693096, Length=211, Percent_Identity=27.4881516587678, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29490; Mature: 29490

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNNNDTLIQSVDRALRILDTFSLKEKELGVTEIANRLGLHKSTVFGLLRTLEHWGYVE
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
QNQVTGKYRLGLKLLELGNRVKEGLDLRAVALPFLQDLVERYGETVHLAVHDRGEIVYIE
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEE
KVEGPNAIRMYSQIGRRAPMHCTGVGKAILAFRPEKEIEEIIRTKGLKYFTPNTITDPKK
ECCCCCHHHHHHHHCCCCCCEEHHCCCHHHEECCHHHHHHHHHHCCCEEECCCCCCCHHH
LHEELSLIRENGYSLDREEIEIGLRCVAAPIRDSQNTVVAAISVAGPSIRMTEEKIQELI
HHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCEEEEEEEECCCCEEECHHHHHHHH
VSVKEAALEISKRLGYQTSSR
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKNNNDTLIQSVDRALRILDTFSLKEKELGVTEIANRLGLHKSTVFGLLRTLEHWGYVE
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
QNQVTGKYRLGLKLLELGNRVKEGLDLRAVALPFLQDLVERYGETVHLAVHDRGEIVYIE
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEE
KVEGPNAIRMYSQIGRRAPMHCTGVGKAILAFRPEKEIEEIIRTKGLKYFTPNTITDPKK
ECCCCCHHHHHHHHCCCCCCEEHHCCCHHHEECCHHHHHHHHHHCCCEEECCCCCCCHHH
LHEELSLIRENGYSLDREEIEIGLRCVAAPIRDSQNTVVAAISVAGPSIRMTEEKIQELI
HHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCEEEEEEEECCCCEEECHHHHHHHH
VSVKEAALEISKRLGYQTSSR
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]